Association of single nucleotide polymorphism in NLRC3, NLRC5, HIP1, and LRP8 genes with fecal egg counts in goats naturally infected with Haemonchus contortus
Haemonchus contortus is a common, intractably pathogenic and economically important gastrointestinal nematode for goat producers worldwide, especially in tropical and subtropical regions. The objective of this study is to identify single nucleotide polymorphisms (SNPs) of 12 candidate goat genes mai...
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| creator | Omar, Abdullah Ibne Alam, Mahmuda Bilkis Bintee Notter, David Russell Zhao, Shuhong Faruque, Md.Omar Thi, Thuy Nhien Tran Yin, Lilin Li, Jingjin Azmal, Syed Ali Du, Xiaoyong |
| description | Haemonchus contortus
is a common, intractably pathogenic and economically important gastrointestinal nematode for goat producers worldwide, especially in tropical and subtropical regions. The objective of this study is to identify single nucleotide polymorphisms (SNPs) of 12 candidate goat genes mainly related to the innate immune response associated with fecal egg counts (FECs) of
Haemonchus contortus
in goat as an indicator of the level of parasite infection. Phenotypic data including FEC and blood traits were recorded in 189 native goats from China and 191 ones from Bangladesh, respectively. Bangladeshi goats had significantly (
P
< 0.01) lower FEC compared to that of Chinese goats, suggesting higher susceptible and infection rates in Chinese goat populations. FEC was significantly positive correlated with body weight (
r
= 0.64,
P
< 0.01) and hemoglobin (
r
= 0.49,
P
< 0.01) value, but negative with pack cell volume (
r
= − 0.63,
P
< 0.05) in goats. Genotyping of SNPs was performed using a matrix-assisted laser desorption ionization time of flight mass spectrometry assay and a generalized linear model was used to evaluate the association between each SNP and goat FEC trait. Eleven novel SNPs in the
NLRC3
,
NLRC5
,
HIP1
, and
LRP8
, out of 46 variants from these 12 genes, were significantly associated with FEC of goats with a nominal significance level of
P
< 0.05. Of these 11 SNPs, linkage disequilibrium were revealed among SNPs in
LRP8
(
r
2
= 0.87 to 1), between SNPs in
NLRC3
,
NLRC5
, and
HIP1
(
r
2
= 0.96 to 0.99), respectively. Further, haplotypes within
NLRC3
,
NLRC5
, and
HIP1
were significantly associated (
P
< 0.001) with FEC. In artificial challenge trail, quantitative real-time PCR exposed that the relative expression of mRNA was higher in the resistant group for
NLRC3
(
P
< 0.01),
LRP8
and
HIP1
(
P
< 0.001) but lower in the resistant group for
NLRC5
(
P
< 0.0001), compared to the susceptible group. The possible SNP markers and genes identified in this study could be potentially used in marker-assisted selection for breeding local goats breeds resistant to gastrointestinal nematode parasite particularly for
Haemonchus contortus
, and then for improving health and productivity of goat. |
| doi_str_mv | 10.1007/s11250-019-02154-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2325301640</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2416297954</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-38dc7ecb8185f9cd35f111e7dcf18be3b17513369b80123e02c97ab2157c3aaa3</originalsourceid><addsrcrecordid>eNp9kcFu1DAURSMEokPhB1ggS2xYTMDPjsfOshoBU2kEVQVry3FeMqkSe7AdVdOf4VdxmwISC1bPss-5tnyL4jXQ90Cp_BABmKAlhbqkDERV3j0pViAkL2XF1dNiRWlVl5Ws5FnxIsYbSrOmNs-LMw6KKSFhVfy8iNHbwaTBO-I7EgfXj0jcbEf0aWiRHP14mnw4HoY4kcGRL_vrLV8_DLEmu8srWBPjWrK_vlKkR4eR3A7pQDq0ZiTY98T62aV47_be5IUzaQ5mHE95K1MJ28XYGZy8s4c5ZsUlH9IcXxbPOjNGfPU4z4vvnz5-2-7K_dfPl9uLfWm5FKnkqrUSbaNAia62LRcdAKBsbQeqQd6AFMD5pm4UBcaRMltL0-Rfk5YbY_h58W7JPQb_Y8aY9DREi-NoHPo5asaZ4BQ2Fc3o23_QGz8Hl1-nWQUbVstaVJliC2WDjzFgp49hmEw4aaD6vj691KdzffqhPn2XpTeP0XMzYftH-d1XBvgCxHzkegx_7_5P7C-Tt6Y3</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2416297954</pqid></control><display><type>article</type><title>Association of single nucleotide polymorphism in NLRC3, NLRC5, HIP1, and LRP8 genes with fecal egg counts in goats naturally infected with Haemonchus contortus</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Omar, Abdullah Ibne ; Alam, Mahmuda Bilkis Bintee ; Notter, David Russell ; Zhao, Shuhong ; Faruque, Md.Omar ; Thi, Thuy Nhien Tran ; Yin, Lilin ; Li, Jingjin ; Azmal, Syed Ali ; Du, Xiaoyong</creator><creatorcontrib>Omar, Abdullah Ibne ; Alam, Mahmuda Bilkis Bintee ; Notter, David Russell ; Zhao, Shuhong ; Faruque, Md.Omar ; Thi, Thuy Nhien Tran ; Yin, Lilin ; Li, Jingjin ; Azmal, Syed Ali ; Du, Xiaoyong</creatorcontrib><description><![CDATA[Haemonchus contortus
is a common, intractably pathogenic and economically important gastrointestinal nematode for goat producers worldwide, especially in tropical and subtropical regions. The objective of this study is to identify single nucleotide polymorphisms (SNPs) of 12 candidate goat genes mainly related to the innate immune response associated with fecal egg counts (FECs) of
Haemonchus contortus
in goat as an indicator of the level of parasite infection. Phenotypic data including FEC and blood traits were recorded in 189 native goats from China and 191 ones from Bangladesh, respectively. Bangladeshi goats had significantly (
P
< 0.01) lower FEC compared to that of Chinese goats, suggesting higher susceptible and infection rates in Chinese goat populations. FEC was significantly positive correlated with body weight (
r
= 0.64,
P
< 0.01) and hemoglobin (
r
= 0.49,
P
< 0.01) value, but negative with pack cell volume (
r
= − 0.63,
P
< 0.05) in goats. Genotyping of SNPs was performed using a matrix-assisted laser desorption ionization time of flight mass spectrometry assay and a generalized linear model was used to evaluate the association between each SNP and goat FEC trait. Eleven novel SNPs in the
NLRC3
,
NLRC5
,
HIP1
, and
LRP8
, out of 46 variants from these 12 genes, were significantly associated with FEC of goats with a nominal significance level of
P
< 0.05. Of these 11 SNPs, linkage disequilibrium were revealed among SNPs in
LRP8
(
r
2
= 0.87 to 1), between SNPs in
NLRC3
,
NLRC5
, and
HIP1
(
r
2
= 0.96 to 0.99), respectively. Further, haplotypes within
NLRC3
,
NLRC5
, and
HIP1
were significantly associated (
P
< 0.001) with FEC. In artificial challenge trail, quantitative real-time PCR exposed that the relative expression of mRNA was higher in the resistant group for
NLRC3
(
P
< 0.01),
LRP8
and
HIP1
(
P
< 0.001) but lower in the resistant group for
NLRC5
(
P
< 0.0001), compared to the susceptible group. The possible SNP markers and genes identified in this study could be potentially used in marker-assisted selection for breeding local goats breeds resistant to gastrointestinal nematode parasite particularly for
Haemonchus contortus
, and then for improving health and productivity of goat.]]></description><identifier>ISSN: 0049-4747</identifier><identifier>EISSN: 1573-7438</identifier><identifier>DOI: 10.1007/s11250-019-02154-z</identifier><identifier>PMID: 31828571</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal husbandry ; Animal populations ; Animals ; Biomarkers ; Biomedical and Life Sciences ; Body weight ; Body Weight - genetics ; Breeding ; Cell size ; Economic importance ; Feces - parasitology ; Female ; Gene expression ; Gene polymorphism ; Generalized linear models ; Genes ; Genetic Predisposition to Disease ; Genotyping ; Goat Diseases - genetics ; Goat Diseases - parasitology ; Goats ; Goats - genetics ; Haemonchiasis - veterinary ; Haemonchus ; Haemonchus contortus ; Haplotypes ; Hemoglobin ; Immune response ; Immunity, Innate ; Innate immunity ; Intestinal Diseases, Parasitic - genetics ; Intestinal Diseases, Parasitic - parasitology ; Intestinal Diseases, Parasitic - veterinary ; Intestinal parasites ; Ionization ; Ions ; Life Sciences ; Linkage disequilibrium ; Marker-assisted selection ; Mass spectrometry ; Mass spectroscopy ; mRNA ; Nematodes ; Nucleotides ; Parasite Egg Count - veterinary ; Parasites ; Pest resistance ; Polymorphism ; Polymorphism, Single Nucleotide ; Regular Articles ; Single-nucleotide polymorphism ; Statistical models ; Veterinary Medicine/Veterinary Science ; Zoology</subject><ispartof>Tropical animal health and production, 2020-07, Vol.52 (4), p.1583-1598</ispartof><rights>Springer Nature B.V. 2019</rights><rights>Springer Nature B.V. 2019.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-38dc7ecb8185f9cd35f111e7dcf18be3b17513369b80123e02c97ab2157c3aaa3</citedby><cites>FETCH-LOGICAL-c375t-38dc7ecb8185f9cd35f111e7dcf18be3b17513369b80123e02c97ab2157c3aaa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11250-019-02154-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11250-019-02154-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31828571$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Omar, Abdullah Ibne</creatorcontrib><creatorcontrib>Alam, Mahmuda Bilkis Bintee</creatorcontrib><creatorcontrib>Notter, David Russell</creatorcontrib><creatorcontrib>Zhao, Shuhong</creatorcontrib><creatorcontrib>Faruque, Md.Omar</creatorcontrib><creatorcontrib>Thi, Thuy Nhien Tran</creatorcontrib><creatorcontrib>Yin, Lilin</creatorcontrib><creatorcontrib>Li, Jingjin</creatorcontrib><creatorcontrib>Azmal, Syed Ali</creatorcontrib><creatorcontrib>Du, Xiaoyong</creatorcontrib><title>Association of single nucleotide polymorphism in NLRC3, NLRC5, HIP1, and LRP8 genes with fecal egg counts in goats naturally infected with Haemonchus contortus</title><title>Tropical animal health and production</title><addtitle>Trop Anim Health Prod</addtitle><addtitle>Trop Anim Health Prod</addtitle><description><![CDATA[Haemonchus contortus
is a common, intractably pathogenic and economically important gastrointestinal nematode for goat producers worldwide, especially in tropical and subtropical regions. The objective of this study is to identify single nucleotide polymorphisms (SNPs) of 12 candidate goat genes mainly related to the innate immune response associated with fecal egg counts (FECs) of
Haemonchus contortus
in goat as an indicator of the level of parasite infection. Phenotypic data including FEC and blood traits were recorded in 189 native goats from China and 191 ones from Bangladesh, respectively. Bangladeshi goats had significantly (
P
< 0.01) lower FEC compared to that of Chinese goats, suggesting higher susceptible and infection rates in Chinese goat populations. FEC was significantly positive correlated with body weight (
r
= 0.64,
P
< 0.01) and hemoglobin (
r
= 0.49,
P
< 0.01) value, but negative with pack cell volume (
r
= − 0.63,
P
< 0.05) in goats. Genotyping of SNPs was performed using a matrix-assisted laser desorption ionization time of flight mass spectrometry assay and a generalized linear model was used to evaluate the association between each SNP and goat FEC trait. Eleven novel SNPs in the
NLRC3
,
NLRC5
,
HIP1
, and
LRP8
, out of 46 variants from these 12 genes, were significantly associated with FEC of goats with a nominal significance level of
P
< 0.05. Of these 11 SNPs, linkage disequilibrium were revealed among SNPs in
LRP8
(
r
2
= 0.87 to 1), between SNPs in
NLRC3
,
NLRC5
, and
HIP1
(
r
2
= 0.96 to 0.99), respectively. Further, haplotypes within
NLRC3
,
NLRC5
, and
HIP1
were significantly associated (
P
< 0.001) with FEC. In artificial challenge trail, quantitative real-time PCR exposed that the relative expression of mRNA was higher in the resistant group for
NLRC3
(
P
< 0.01),
LRP8
and
HIP1
(
P
< 0.001) but lower in the resistant group for
NLRC5
(
P
< 0.0001), compared to the susceptible group. The possible SNP markers and genes identified in this study could be potentially used in marker-assisted selection for breeding local goats breeds resistant to gastrointestinal nematode parasite particularly for
Haemonchus contortus
, and then for improving health and productivity of goat.]]></description><subject>Animal husbandry</subject><subject>Animal populations</subject><subject>Animals</subject><subject>Biomarkers</subject><subject>Biomedical and Life Sciences</subject><subject>Body weight</subject><subject>Body Weight - genetics</subject><subject>Breeding</subject><subject>Cell size</subject><subject>Economic importance</subject><subject>Feces - parasitology</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene polymorphism</subject><subject>Generalized linear models</subject><subject>Genes</subject><subject>Genetic Predisposition to Disease</subject><subject>Genotyping</subject><subject>Goat Diseases - genetics</subject><subject>Goat Diseases - parasitology</subject><subject>Goats</subject><subject>Goats - genetics</subject><subject>Haemonchiasis - veterinary</subject><subject>Haemonchus</subject><subject>Haemonchus contortus</subject><subject>Haplotypes</subject><subject>Hemoglobin</subject><subject>Immune response</subject><subject>Immunity, Innate</subject><subject>Innate immunity</subject><subject>Intestinal Diseases, Parasitic - genetics</subject><subject>Intestinal Diseases, Parasitic - parasitology</subject><subject>Intestinal Diseases, Parasitic - veterinary</subject><subject>Intestinal parasites</subject><subject>Ionization</subject><subject>Ions</subject><subject>Life Sciences</subject><subject>Linkage disequilibrium</subject><subject>Marker-assisted selection</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>mRNA</subject><subject>Nematodes</subject><subject>Nucleotides</subject><subject>Parasite Egg Count - veterinary</subject><subject>Parasites</subject><subject>Pest resistance</subject><subject>Polymorphism</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Regular Articles</subject><subject>Single-nucleotide polymorphism</subject><subject>Statistical models</subject><subject>Veterinary Medicine/Veterinary Science</subject><subject>Zoology</subject><issn>0049-4747</issn><issn>1573-7438</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kcFu1DAURSMEokPhB1ggS2xYTMDPjsfOshoBU2kEVQVry3FeMqkSe7AdVdOf4VdxmwISC1bPss-5tnyL4jXQ90Cp_BABmKAlhbqkDERV3j0pViAkL2XF1dNiRWlVl5Ws5FnxIsYbSrOmNs-LMw6KKSFhVfy8iNHbwaTBO-I7EgfXj0jcbEf0aWiRHP14mnw4HoY4kcGRL_vrLV8_DLEmu8srWBPjWrK_vlKkR4eR3A7pQDq0ZiTY98T62aV47_be5IUzaQ5mHE95K1MJ28XYGZy8s4c5ZsUlH9IcXxbPOjNGfPU4z4vvnz5-2-7K_dfPl9uLfWm5FKnkqrUSbaNAia62LRcdAKBsbQeqQd6AFMD5pm4UBcaRMltL0-Rfk5YbY_h58W7JPQb_Y8aY9DREi-NoHPo5asaZ4BQ2Fc3o23_QGz8Hl1-nWQUbVstaVJliC2WDjzFgp49hmEw4aaD6vj691KdzffqhPn2XpTeP0XMzYftH-d1XBvgCxHzkegx_7_5P7C-Tt6Y3</recordid><startdate>20200701</startdate><enddate>20200701</enddate><creator>Omar, Abdullah Ibne</creator><creator>Alam, Mahmuda Bilkis Bintee</creator><creator>Notter, David Russell</creator><creator>Zhao, Shuhong</creator><creator>Faruque, Md.Omar</creator><creator>Thi, Thuy Nhien Tran</creator><creator>Yin, Lilin</creator><creator>Li, Jingjin</creator><creator>Azmal, Syed Ali</creator><creator>Du, Xiaoyong</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U7</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20200701</creationdate><title>Association of single nucleotide polymorphism in NLRC3, NLRC5, HIP1, and LRP8 genes with fecal egg counts in goats naturally infected with Haemonchus contortus</title><author>Omar, Abdullah Ibne ; Alam, Mahmuda Bilkis Bintee ; Notter, David Russell ; Zhao, Shuhong ; Faruque, Md.Omar ; Thi, Thuy Nhien Tran ; Yin, Lilin ; Li, Jingjin ; Azmal, Syed Ali ; Du, Xiaoyong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-38dc7ecb8185f9cd35f111e7dcf18be3b17513369b80123e02c97ab2157c3aaa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animal husbandry</topic><topic>Animal populations</topic><topic>Animals</topic><topic>Biomarkers</topic><topic>Biomedical and Life Sciences</topic><topic>Body weight</topic><topic>Body Weight - genetics</topic><topic>Breeding</topic><topic>Cell size</topic><topic>Economic importance</topic><topic>Feces - parasitology</topic><topic>Female</topic><topic>Gene expression</topic><topic>Gene polymorphism</topic><topic>Generalized linear models</topic><topic>Genes</topic><topic>Genetic Predisposition to Disease</topic><topic>Genotyping</topic><topic>Goat Diseases - genetics</topic><topic>Goat Diseases - parasitology</topic><topic>Goats</topic><topic>Goats - genetics</topic><topic>Haemonchiasis - veterinary</topic><topic>Haemonchus</topic><topic>Haemonchus contortus</topic><topic>Haplotypes</topic><topic>Hemoglobin</topic><topic>Immune response</topic><topic>Immunity, Innate</topic><topic>Innate immunity</topic><topic>Intestinal Diseases, Parasitic - genetics</topic><topic>Intestinal Diseases, Parasitic - parasitology</topic><topic>Intestinal Diseases, Parasitic - veterinary</topic><topic>Intestinal parasites</topic><topic>Ionization</topic><topic>Ions</topic><topic>Life Sciences</topic><topic>Linkage disequilibrium</topic><topic>Marker-assisted selection</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>mRNA</topic><topic>Nematodes</topic><topic>Nucleotides</topic><topic>Parasite Egg Count - veterinary</topic><topic>Parasites</topic><topic>Pest resistance</topic><topic>Polymorphism</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Regular Articles</topic><topic>Single-nucleotide polymorphism</topic><topic>Statistical models</topic><topic>Veterinary Medicine/Veterinary Science</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Omar, Abdullah Ibne</creatorcontrib><creatorcontrib>Alam, Mahmuda Bilkis Bintee</creatorcontrib><creatorcontrib>Notter, David Russell</creatorcontrib><creatorcontrib>Zhao, Shuhong</creatorcontrib><creatorcontrib>Faruque, Md.Omar</creatorcontrib><creatorcontrib>Thi, Thuy Nhien Tran</creatorcontrib><creatorcontrib>Yin, Lilin</creatorcontrib><creatorcontrib>Li, Jingjin</creatorcontrib><creatorcontrib>Azmal, Syed Ali</creatorcontrib><creatorcontrib>Du, Xiaoyong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Tropical animal health and production</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Omar, Abdullah Ibne</au><au>Alam, Mahmuda Bilkis Bintee</au><au>Notter, David Russell</au><au>Zhao, Shuhong</au><au>Faruque, Md.Omar</au><au>Thi, Thuy Nhien Tran</au><au>Yin, Lilin</au><au>Li, Jingjin</au><au>Azmal, Syed Ali</au><au>Du, Xiaoyong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Association of single nucleotide polymorphism in NLRC3, NLRC5, HIP1, and LRP8 genes with fecal egg counts in goats naturally infected with Haemonchus contortus</atitle><jtitle>Tropical animal health and production</jtitle><stitle>Trop Anim Health Prod</stitle><addtitle>Trop Anim Health Prod</addtitle><date>2020-07-01</date><risdate>2020</risdate><volume>52</volume><issue>4</issue><spage>1583</spage><epage>1598</epage><pages>1583-1598</pages><issn>0049-4747</issn><eissn>1573-7438</eissn><abstract><![CDATA[Haemonchus contortus
is a common, intractably pathogenic and economically important gastrointestinal nematode for goat producers worldwide, especially in tropical and subtropical regions. The objective of this study is to identify single nucleotide polymorphisms (SNPs) of 12 candidate goat genes mainly related to the innate immune response associated with fecal egg counts (FECs) of
Haemonchus contortus
in goat as an indicator of the level of parasite infection. Phenotypic data including FEC and blood traits were recorded in 189 native goats from China and 191 ones from Bangladesh, respectively. Bangladeshi goats had significantly (
P
< 0.01) lower FEC compared to that of Chinese goats, suggesting higher susceptible and infection rates in Chinese goat populations. FEC was significantly positive correlated with body weight (
r
= 0.64,
P
< 0.01) and hemoglobin (
r
= 0.49,
P
< 0.01) value, but negative with pack cell volume (
r
= − 0.63,
P
< 0.05) in goats. Genotyping of SNPs was performed using a matrix-assisted laser desorption ionization time of flight mass spectrometry assay and a generalized linear model was used to evaluate the association between each SNP and goat FEC trait. Eleven novel SNPs in the
NLRC3
,
NLRC5
,
HIP1
, and
LRP8
, out of 46 variants from these 12 genes, were significantly associated with FEC of goats with a nominal significance level of
P
< 0.05. Of these 11 SNPs, linkage disequilibrium were revealed among SNPs in
LRP8
(
r
2
= 0.87 to 1), between SNPs in
NLRC3
,
NLRC5
, and
HIP1
(
r
2
= 0.96 to 0.99), respectively. Further, haplotypes within
NLRC3
,
NLRC5
, and
HIP1
were significantly associated (
P
< 0.001) with FEC. In artificial challenge trail, quantitative real-time PCR exposed that the relative expression of mRNA was higher in the resistant group for
NLRC3
(
P
< 0.01),
LRP8
and
HIP1
(
P
< 0.001) but lower in the resistant group for
NLRC5
(
P
< 0.0001), compared to the susceptible group. The possible SNP markers and genes identified in this study could be potentially used in marker-assisted selection for breeding local goats breeds resistant to gastrointestinal nematode parasite particularly for
Haemonchus contortus
, and then for improving health and productivity of goat.]]></abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>31828571</pmid><doi>10.1007/s11250-019-02154-z</doi><tpages>16</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0049-4747 |
| ispartof | Tropical animal health and production, 2020-07, Vol.52 (4), p.1583-1598 |
| issn | 0049-4747 1573-7438 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2325301640 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animal husbandry Animal populations Animals Biomarkers Biomedical and Life Sciences Body weight Body Weight - genetics Breeding Cell size Economic importance Feces - parasitology Female Gene expression Gene polymorphism Generalized linear models Genes Genetic Predisposition to Disease Genotyping Goat Diseases - genetics Goat Diseases - parasitology Goats Goats - genetics Haemonchiasis - veterinary Haemonchus Haemonchus contortus Haplotypes Hemoglobin Immune response Immunity, Innate Innate immunity Intestinal Diseases, Parasitic - genetics Intestinal Diseases, Parasitic - parasitology Intestinal Diseases, Parasitic - veterinary Intestinal parasites Ionization Ions Life Sciences Linkage disequilibrium Marker-assisted selection Mass spectrometry Mass spectroscopy mRNA Nematodes Nucleotides Parasite Egg Count - veterinary Parasites Pest resistance Polymorphism Polymorphism, Single Nucleotide Regular Articles Single-nucleotide polymorphism Statistical models Veterinary Medicine/Veterinary Science Zoology |
| title | Association of single nucleotide polymorphism in NLRC3, NLRC5, HIP1, and LRP8 genes with fecal egg counts in goats naturally infected with Haemonchus contortus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T02%3A33%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Association%20of%20single%20nucleotide%20polymorphism%20in%20NLRC3,%20NLRC5,%20HIP1,%20and%20LRP8%20genes%20with%20fecal%20egg%20counts%20in%20goats%20naturally%20infected%20with%20Haemonchus%20contortus&rft.jtitle=Tropical%20animal%20health%20and%20production&rft.au=Omar,%20Abdullah%20Ibne&rft.date=2020-07-01&rft.volume=52&rft.issue=4&rft.spage=1583&rft.epage=1598&rft.pages=1583-1598&rft.issn=0049-4747&rft.eissn=1573-7438&rft_id=info:doi/10.1007/s11250-019-02154-z&rft_dat=%3Cproquest_cross%3E2416297954%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2416297954&rft_id=info:pmid/31828571&rfr_iscdi=true |