PepA binds to and negatively regulates esrB to control virulence in the fish pathogen Edwardsiella piscicida
As an important marine fish pathogen, Edwardsiella piscicida infects a broad range of fish species and causes substantial economic losses. The EsrA-EsrB two-component system is essential for the expression of type III and type VI secretion systems (T3/T6SSs), the key virulence determinants in the ba...
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| Veröffentlicht in: | Microbiological research 2020-02, Vol.232, p.126349-126349, Article 126349 |
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| container_title | Microbiological research |
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| creator | Yin, Kaiyu Peng, Yue Ahmed, Moamer A.H. Ma, Jiabao Xu, Rongjing Zhang, Yuanxing Ma, Yue Wang, Qiyao |
| description | As an important marine fish pathogen, Edwardsiella piscicida infects a broad range of fish species and causes substantial economic losses. The EsrA-EsrB two-component system is essential for the expression of type III and type VI secretion systems (T3/T6SSs), the key virulence determinants in the bacterium. In this study, a pull-down assay with the esrB promoter as bait was performed to identify the upstream regulators of esrB. As a result, PepA, a leucyl aminopeptidase, was identified as a repressor of EsrB and T3/T6SS expression. PepA bound to the esrB promoter region and negatively regulated the production of T3/T6SS proteins in early stages. Moreover, PepA was found to affect the in vivo colonization of E. piscicida in turbot livers through the regulation of EsrB expression. Collectively, our results enhance the understanding of the virulence regulatory network and in vivo colonization mechanism of E. piscicida.
One sentence summary: PepA regulates EsrB expression in Edwardsiella piscicida. |
| doi_str_mv | 10.1016/j.micres.2019.126349 |
| format | Article |
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One sentence summary: PepA regulates EsrB expression in Edwardsiella piscicida.</description><identifier>ISSN: 0944-5013</identifier><identifier>EISSN: 1618-0623</identifier><identifier>DOI: 10.1016/j.micres.2019.126349</identifier><identifier>PMID: 31816594</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Animals ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Edwardsiella - genetics ; Edwardsiella - metabolism ; Edwardsiella piscicida ; Electrophoretic Mobility Shift Assay - veterinary ; Enterobacteriaceae Infections - microbiology ; Enterobacteriaceae Infections - veterinary ; EsrB ; Fish Diseases - microbiology ; Gene Expression Regulation, Bacterial ; In vivo colonization ; Leucyl aminopeptidase ; Leucyl Aminopeptidase - genetics ; Leucyl Aminopeptidase - metabolism ; Promoter Regions, Genetic ; Pull-down ; Reactive Oxygen Species ; Type III Secretion Systems - metabolism ; Type VI Secretion Systems - metabolism ; Virulence - genetics ; Virulence Factors - genetics ; Virulence Factors - metabolism</subject><ispartof>Microbiological research, 2020-02, Vol.232, p.126349-126349, Article 126349</ispartof><rights>2019 Elsevier GmbH</rights><rights>Copyright © 2019 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-a94ad17b8f3739f76a7b36916b4538b07000aa2add60fd086668d17bde5af3053</citedby><cites>FETCH-LOGICAL-c362t-a94ad17b8f3739f76a7b36916b4538b07000aa2add60fd086668d17bde5af3053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0944501319307657$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31816594$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yin, Kaiyu</creatorcontrib><creatorcontrib>Peng, Yue</creatorcontrib><creatorcontrib>Ahmed, Moamer A.H.</creatorcontrib><creatorcontrib>Ma, Jiabao</creatorcontrib><creatorcontrib>Xu, Rongjing</creatorcontrib><creatorcontrib>Zhang, Yuanxing</creatorcontrib><creatorcontrib>Ma, Yue</creatorcontrib><creatorcontrib>Wang, Qiyao</creatorcontrib><title>PepA binds to and negatively regulates esrB to control virulence in the fish pathogen Edwardsiella piscicida</title><title>Microbiological research</title><addtitle>Microbiol Res</addtitle><description>As an important marine fish pathogen, Edwardsiella piscicida infects a broad range of fish species and causes substantial economic losses. The EsrA-EsrB two-component system is essential for the expression of type III and type VI secretion systems (T3/T6SSs), the key virulence determinants in the bacterium. In this study, a pull-down assay with the esrB promoter as bait was performed to identify the upstream regulators of esrB. As a result, PepA, a leucyl aminopeptidase, was identified as a repressor of EsrB and T3/T6SS expression. PepA bound to the esrB promoter region and negatively regulated the production of T3/T6SS proteins in early stages. Moreover, PepA was found to affect the in vivo colonization of E. piscicida in turbot livers through the regulation of EsrB expression. Collectively, our results enhance the understanding of the virulence regulatory network and in vivo colonization mechanism of E. piscicida.
One sentence summary: PepA regulates EsrB expression in Edwardsiella piscicida.</description><subject>Animals</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Edwardsiella - genetics</subject><subject>Edwardsiella - metabolism</subject><subject>Edwardsiella piscicida</subject><subject>Electrophoretic Mobility Shift Assay - veterinary</subject><subject>Enterobacteriaceae Infections - microbiology</subject><subject>Enterobacteriaceae Infections - veterinary</subject><subject>EsrB</subject><subject>Fish Diseases - microbiology</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>In vivo colonization</subject><subject>Leucyl aminopeptidase</subject><subject>Leucyl Aminopeptidase - genetics</subject><subject>Leucyl Aminopeptidase - metabolism</subject><subject>Promoter Regions, Genetic</subject><subject>Pull-down</subject><subject>Reactive Oxygen Species</subject><subject>Type III Secretion Systems - metabolism</subject><subject>Type VI Secretion Systems - metabolism</subject><subject>Virulence - genetics</subject><subject>Virulence Factors - genetics</subject><subject>Virulence Factors - metabolism</subject><issn>0944-5013</issn><issn>1618-0623</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOwzAQRS0EglL4A4S8ZJPiV5x4gwSoPKRKsIC15cST1lWaBNsp4u9JFGDJajZn5t45CF1QsqCEyuvtYudKD2HBCFULyiQX6gDNqKR5QiTjh2hGlBBJSig_QachbAmhQuXsGJ1wmlOZKjFD9St0t7hwjQ04ttg0FjewNtHtof7CHtZ9bSIEDMHfjUDZNtG3Nd4739fQlIBdg-MGcOXCBncmbto1NHhpP423wUFdG9y5ULrSWXOGjipTBzj_mXP0_rB8u39KVi-Pz_e3q6TkksXEKGEszYq84hlXVSZNVnCpqCxEyvOCZIQQY5ixVpLKklxKmY-8hdRUnKR8jq6mu51vP3oIUe-GCmOXBto-aMYZF7kSKRtQMaGlb0PwUOnOu53xX5oSPXrWWz151qNnPXke1i5_EvpiB_Zv6VfsANxMAAx_7h14PTgYfVnnoYzatu7_hG_E_pFD</recordid><startdate>202002</startdate><enddate>202002</enddate><creator>Yin, Kaiyu</creator><creator>Peng, Yue</creator><creator>Ahmed, Moamer A.H.</creator><creator>Ma, Jiabao</creator><creator>Xu, Rongjing</creator><creator>Zhang, Yuanxing</creator><creator>Ma, Yue</creator><creator>Wang, Qiyao</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202002</creationdate><title>PepA binds to and negatively regulates esrB to control virulence in the fish pathogen Edwardsiella piscicida</title><author>Yin, Kaiyu ; Peng, Yue ; Ahmed, Moamer A.H. ; Ma, Jiabao ; Xu, Rongjing ; Zhang, Yuanxing ; Ma, Yue ; Wang, Qiyao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-a94ad17b8f3739f76a7b36916b4538b07000aa2add60fd086668d17bde5af3053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Edwardsiella - genetics</topic><topic>Edwardsiella - metabolism</topic><topic>Edwardsiella piscicida</topic><topic>Electrophoretic Mobility Shift Assay - veterinary</topic><topic>Enterobacteriaceae Infections - microbiology</topic><topic>Enterobacteriaceae Infections - veterinary</topic><topic>EsrB</topic><topic>Fish Diseases - microbiology</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>In vivo colonization</topic><topic>Leucyl aminopeptidase</topic><topic>Leucyl Aminopeptidase - genetics</topic><topic>Leucyl Aminopeptidase - metabolism</topic><topic>Promoter Regions, Genetic</topic><topic>Pull-down</topic><topic>Reactive Oxygen Species</topic><topic>Type III Secretion Systems - metabolism</topic><topic>Type VI Secretion Systems - metabolism</topic><topic>Virulence - genetics</topic><topic>Virulence Factors - genetics</topic><topic>Virulence Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yin, Kaiyu</creatorcontrib><creatorcontrib>Peng, Yue</creatorcontrib><creatorcontrib>Ahmed, Moamer A.H.</creatorcontrib><creatorcontrib>Ma, Jiabao</creatorcontrib><creatorcontrib>Xu, Rongjing</creatorcontrib><creatorcontrib>Zhang, Yuanxing</creatorcontrib><creatorcontrib>Ma, Yue</creatorcontrib><creatorcontrib>Wang, Qiyao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microbiological research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yin, Kaiyu</au><au>Peng, Yue</au><au>Ahmed, Moamer A.H.</au><au>Ma, Jiabao</au><au>Xu, Rongjing</au><au>Zhang, Yuanxing</au><au>Ma, Yue</au><au>Wang, Qiyao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PepA binds to and negatively regulates esrB to control virulence in the fish pathogen Edwardsiella piscicida</atitle><jtitle>Microbiological research</jtitle><addtitle>Microbiol Res</addtitle><date>2020-02</date><risdate>2020</risdate><volume>232</volume><spage>126349</spage><epage>126349</epage><pages>126349-126349</pages><artnum>126349</artnum><issn>0944-5013</issn><eissn>1618-0623</eissn><abstract>As an important marine fish pathogen, Edwardsiella piscicida infects a broad range of fish species and causes substantial economic losses. The EsrA-EsrB two-component system is essential for the expression of type III and type VI secretion systems (T3/T6SSs), the key virulence determinants in the bacterium. In this study, a pull-down assay with the esrB promoter as bait was performed to identify the upstream regulators of esrB. As a result, PepA, a leucyl aminopeptidase, was identified as a repressor of EsrB and T3/T6SS expression. PepA bound to the esrB promoter region and negatively regulated the production of T3/T6SS proteins in early stages. Moreover, PepA was found to affect the in vivo colonization of E. piscicida in turbot livers through the regulation of EsrB expression. Collectively, our results enhance the understanding of the virulence regulatory network and in vivo colonization mechanism of E. piscicida.
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| subjects | Animals Bacterial Proteins - genetics Bacterial Proteins - metabolism Edwardsiella - genetics Edwardsiella - metabolism Edwardsiella piscicida Electrophoretic Mobility Shift Assay - veterinary Enterobacteriaceae Infections - microbiology Enterobacteriaceae Infections - veterinary EsrB Fish Diseases - microbiology Gene Expression Regulation, Bacterial In vivo colonization Leucyl aminopeptidase Leucyl Aminopeptidase - genetics Leucyl Aminopeptidase - metabolism Promoter Regions, Genetic Pull-down Reactive Oxygen Species Type III Secretion Systems - metabolism Type VI Secretion Systems - metabolism Virulence - genetics Virulence Factors - genetics Virulence Factors - metabolism |
| title | PepA binds to and negatively regulates esrB to control virulence in the fish pathogen Edwardsiella piscicida |
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