Stem cell‐derived conditioned media from human exfoliated deciduous teeth promote bone regeneration
Objectives Cleft lip and palate (CL/P) are common congenital orofacial anomalies. Autogenous iliac bone grafting closes alveolar cleft defects but requires surgical intervention. Mesenchymal stem cell culture supernatant can regenerate tissues via paracrine activity. However, little is known about t...
Gespeichert in:
| Veröffentlicht in: | Oral diseases 2020-03, Vol.26 (2), p.381-390 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 390 |
|---|---|
| container_issue | 2 |
| container_start_page | 381 |
| container_title | Oral diseases |
| container_volume | 26 |
| creator | Hiraki, Tomoka Kunimatsu, Ryo Nakajima, Kengo Abe, Takaharu Yamada, Sakura Rikitake, Kodai Tanimoto, Kotaro |
| description | Objectives
Cleft lip and palate (CL/P) are common congenital orofacial anomalies. Autogenous iliac bone grafting closes alveolar cleft defects but requires surgical intervention. Mesenchymal stem cell culture supernatant can regenerate tissues via paracrine activity. However, little is known about the bone‐regenerative effects of stem cells from human exfoliated deciduous teeth (SHED) and conditioned media (CM). Our aim was to address this.
Materials and methods
Stem cells were isolated from primary tooth pulp and cultured. Defects were made in calvariae of immunodeficient mice and implanted with stem cell‐ or CM‐containing atelocollagen. Regenerated bone was analysed by microcomputed tomography, haematoxylin–eosin and Masson's trichrome staining. Vascular endothelial growth factor, CD31 and CD34 expression were confirmed by immunohistochemistry, and the presence of several proteins and growth factors was verified in SHED‐CM.
Results
Bone regeneration was enhanced in defects treated with stem cells and CM compared to that in controls 8 weeks after transplantation. Mature bone formation and angiogenesis were confirmed with CM but not with stem cells or in controls. Secretome analysis using multiple cytokine assays revealed that SHED‐CM contained tissue‐regenerating factors with roles in angiogenesis and osteogenesis.
Conclusion
CM non‐invasively regenerate bone and might be effective to reconstruct alveolar clefts in CL/P patients. |
| doi_str_mv | 10.1111/odi.13244 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2322745582</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2322745582</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4194-7133aba679d5152cbcdb3db7a9cadab829b7f2359be7fcc29641cf4887e7e9693</originalsourceid><addsrcrecordid>eNp1kMtKxDAUhoMoOl4WvoAE3Oii2iRN0yzF64AwCxXclVxONUPbjGnrZecj-Iw-iRlndCGYTQ7ky8d_foR2SXpE4jn21h0RRrNsBY1InpIkLShfjTPjWcIpu99Am103TVMiJKPraIORIiJUjhDc9NBgA3X9-f5hIbhnsNj41rre-TbODVincBV8gx-HRrUYXitfO9XHNwvG2cEPHe4B-kc8i5TvAev4Ewd4gBaCmnu20Vql6g52lvcWurs4vz29Sq4nl-PTk-vEZERmiSCMKa1yIS0nnBptrGZWCyWNskoXVGpRUcalBlEZQ2WeEVNlRSFAgMwl20IHC29M8jRA15eN6-bLqRZizJIySkXGeUEjuv8HnfohtDFdpHjOJRfFXHi4oEzwXRegKmfBNSq8lSQt592Xsfvyu_vI7i2Ng46t_ZI_ZUfgeAG8uBre_jeVk7PxQvkFY-qQCA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2356595789</pqid></control><display><type>article</type><title>Stem cell‐derived conditioned media from human exfoliated deciduous teeth promote bone regeneration</title><source>MEDLINE</source><source>Wiley Journals</source><creator>Hiraki, Tomoka ; Kunimatsu, Ryo ; Nakajima, Kengo ; Abe, Takaharu ; Yamada, Sakura ; Rikitake, Kodai ; Tanimoto, Kotaro</creator><creatorcontrib>Hiraki, Tomoka ; Kunimatsu, Ryo ; Nakajima, Kengo ; Abe, Takaharu ; Yamada, Sakura ; Rikitake, Kodai ; Tanimoto, Kotaro</creatorcontrib><description>Objectives
Cleft lip and palate (CL/P) are common congenital orofacial anomalies. Autogenous iliac bone grafting closes alveolar cleft defects but requires surgical intervention. Mesenchymal stem cell culture supernatant can regenerate tissues via paracrine activity. However, little is known about the bone‐regenerative effects of stem cells from human exfoliated deciduous teeth (SHED) and conditioned media (CM). Our aim was to address this.
Materials and methods
Stem cells were isolated from primary tooth pulp and cultured. Defects were made in calvariae of immunodeficient mice and implanted with stem cell‐ or CM‐containing atelocollagen. Regenerated bone was analysed by microcomputed tomography, haematoxylin–eosin and Masson's trichrome staining. Vascular endothelial growth factor, CD31 and CD34 expression were confirmed by immunohistochemistry, and the presence of several proteins and growth factors was verified in SHED‐CM.
Results
Bone regeneration was enhanced in defects treated with stem cells and CM compared to that in controls 8 weeks after transplantation. Mature bone formation and angiogenesis were confirmed with CM but not with stem cells or in controls. Secretome analysis using multiple cytokine assays revealed that SHED‐CM contained tissue‐regenerating factors with roles in angiogenesis and osteogenesis.
Conclusion
CM non‐invasively regenerate bone and might be effective to reconstruct alveolar clefts in CL/P patients.</description><identifier>ISSN: 1354-523X</identifier><identifier>EISSN: 1601-0825</identifier><identifier>DOI: 10.1111/odi.13244</identifier><identifier>PMID: 31808229</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>Alveolar bone ; Angiogenesis ; Animals ; Atelocollagen ; Bone grafts ; Bone growth ; bone regeneration ; Bone Regeneration - drug effects ; CD34 antigen ; Cell culture ; Child ; Cleft Lip - surgery ; Cleft lip/palate ; Cleft Palate - surgery ; Computed tomography ; conditioned media ; Culture Media, Conditioned - pharmacology ; Defects ; Dental pulp ; Dental Pulp - physiology ; Dentistry ; exfoliated deciduous teeth ; Growth factors ; Humans ; Immunodeficiency ; Immunohistochemistry ; Male ; Mesenchyme ; Mice ; Mice, Inbred BALB C ; Osteogenesis ; Osteogenesis - drug effects ; Paracrine signalling ; Regeneration ; Secretome ; Stem cell transplantation ; Stem cells ; Stem Cells - physiology ; Tooth Exfoliation ; Tooth, Deciduous - cytology ; Vascular endothelial growth factor ; Vascular Endothelial Growth Factor A - metabolism ; X-Ray Microtomography</subject><ispartof>Oral diseases, 2020-03, Vol.26 (2), p.381-390</ispartof><rights>2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved</rights><rights>2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved.</rights><rights>Copyright © 2020 John Wiley & Sons A/S</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4194-7133aba679d5152cbcdb3db7a9cadab829b7f2359be7fcc29641cf4887e7e9693</citedby><cites>FETCH-LOGICAL-c4194-7133aba679d5152cbcdb3db7a9cadab829b7f2359be7fcc29641cf4887e7e9693</cites><orcidid>0000-0002-5016-4957</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fodi.13244$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fodi.13244$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31808229$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hiraki, Tomoka</creatorcontrib><creatorcontrib>Kunimatsu, Ryo</creatorcontrib><creatorcontrib>Nakajima, Kengo</creatorcontrib><creatorcontrib>Abe, Takaharu</creatorcontrib><creatorcontrib>Yamada, Sakura</creatorcontrib><creatorcontrib>Rikitake, Kodai</creatorcontrib><creatorcontrib>Tanimoto, Kotaro</creatorcontrib><title>Stem cell‐derived conditioned media from human exfoliated deciduous teeth promote bone regeneration</title><title>Oral diseases</title><addtitle>Oral Dis</addtitle><description>Objectives
Cleft lip and palate (CL/P) are common congenital orofacial anomalies. Autogenous iliac bone grafting closes alveolar cleft defects but requires surgical intervention. Mesenchymal stem cell culture supernatant can regenerate tissues via paracrine activity. However, little is known about the bone‐regenerative effects of stem cells from human exfoliated deciduous teeth (SHED) and conditioned media (CM). Our aim was to address this.
Materials and methods
Stem cells were isolated from primary tooth pulp and cultured. Defects were made in calvariae of immunodeficient mice and implanted with stem cell‐ or CM‐containing atelocollagen. Regenerated bone was analysed by microcomputed tomography, haematoxylin–eosin and Masson's trichrome staining. Vascular endothelial growth factor, CD31 and CD34 expression were confirmed by immunohistochemistry, and the presence of several proteins and growth factors was verified in SHED‐CM.
Results
Bone regeneration was enhanced in defects treated with stem cells and CM compared to that in controls 8 weeks after transplantation. Mature bone formation and angiogenesis were confirmed with CM but not with stem cells or in controls. Secretome analysis using multiple cytokine assays revealed that SHED‐CM contained tissue‐regenerating factors with roles in angiogenesis and osteogenesis.
Conclusion
CM non‐invasively regenerate bone and might be effective to reconstruct alveolar clefts in CL/P patients.</description><subject>Alveolar bone</subject><subject>Angiogenesis</subject><subject>Animals</subject><subject>Atelocollagen</subject><subject>Bone grafts</subject><subject>Bone growth</subject><subject>bone regeneration</subject><subject>Bone Regeneration - drug effects</subject><subject>CD34 antigen</subject><subject>Cell culture</subject><subject>Child</subject><subject>Cleft Lip - surgery</subject><subject>Cleft lip/palate</subject><subject>Cleft Palate - surgery</subject><subject>Computed tomography</subject><subject>conditioned media</subject><subject>Culture Media, Conditioned - pharmacology</subject><subject>Defects</subject><subject>Dental pulp</subject><subject>Dental Pulp - physiology</subject><subject>Dentistry</subject><subject>exfoliated deciduous teeth</subject><subject>Growth factors</subject><subject>Humans</subject><subject>Immunodeficiency</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Mesenchyme</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Osteogenesis</subject><subject>Osteogenesis - drug effects</subject><subject>Paracrine signalling</subject><subject>Regeneration</subject><subject>Secretome</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Stem Cells - physiology</subject><subject>Tooth Exfoliation</subject><subject>Tooth, Deciduous - cytology</subject><subject>Vascular endothelial growth factor</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><subject>X-Ray Microtomography</subject><issn>1354-523X</issn><issn>1601-0825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kMtKxDAUhoMoOl4WvoAE3Oii2iRN0yzF64AwCxXclVxONUPbjGnrZecj-Iw-iRlndCGYTQ7ky8d_foR2SXpE4jn21h0RRrNsBY1InpIkLShfjTPjWcIpu99Am103TVMiJKPraIORIiJUjhDc9NBgA3X9-f5hIbhnsNj41rre-TbODVincBV8gx-HRrUYXitfO9XHNwvG2cEPHe4B-kc8i5TvAev4Ewd4gBaCmnu20Vql6g52lvcWurs4vz29Sq4nl-PTk-vEZERmiSCMKa1yIS0nnBptrGZWCyWNskoXVGpRUcalBlEZQ2WeEVNlRSFAgMwl20IHC29M8jRA15eN6-bLqRZizJIySkXGeUEjuv8HnfohtDFdpHjOJRfFXHi4oEzwXRegKmfBNSq8lSQt592Xsfvyu_vI7i2Ng46t_ZI_ZUfgeAG8uBre_jeVk7PxQvkFY-qQCA</recordid><startdate>202003</startdate><enddate>202003</enddate><creator>Hiraki, Tomoka</creator><creator>Kunimatsu, Ryo</creator><creator>Nakajima, Kengo</creator><creator>Abe, Takaharu</creator><creator>Yamada, Sakura</creator><creator>Rikitake, Kodai</creator><creator>Tanimoto, Kotaro</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5016-4957</orcidid></search><sort><creationdate>202003</creationdate><title>Stem cell‐derived conditioned media from human exfoliated deciduous teeth promote bone regeneration</title><author>Hiraki, Tomoka ; Kunimatsu, Ryo ; Nakajima, Kengo ; Abe, Takaharu ; Yamada, Sakura ; Rikitake, Kodai ; Tanimoto, Kotaro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4194-7133aba679d5152cbcdb3db7a9cadab829b7f2359be7fcc29641cf4887e7e9693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Alveolar bone</topic><topic>Angiogenesis</topic><topic>Animals</topic><topic>Atelocollagen</topic><topic>Bone grafts</topic><topic>Bone growth</topic><topic>bone regeneration</topic><topic>Bone Regeneration - drug effects</topic><topic>CD34 antigen</topic><topic>Cell culture</topic><topic>Child</topic><topic>Cleft Lip - surgery</topic><topic>Cleft lip/palate</topic><topic>Cleft Palate - surgery</topic><topic>Computed tomography</topic><topic>conditioned media</topic><topic>Culture Media, Conditioned - pharmacology</topic><topic>Defects</topic><topic>Dental pulp</topic><topic>Dental Pulp - physiology</topic><topic>Dentistry</topic><topic>exfoliated deciduous teeth</topic><topic>Growth factors</topic><topic>Humans</topic><topic>Immunodeficiency</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Mesenchyme</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Osteogenesis</topic><topic>Osteogenesis - drug effects</topic><topic>Paracrine signalling</topic><topic>Regeneration</topic><topic>Secretome</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Stem Cells - physiology</topic><topic>Tooth Exfoliation</topic><topic>Tooth, Deciduous - cytology</topic><topic>Vascular endothelial growth factor</topic><topic>Vascular Endothelial Growth Factor A - metabolism</topic><topic>X-Ray Microtomography</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hiraki, Tomoka</creatorcontrib><creatorcontrib>Kunimatsu, Ryo</creatorcontrib><creatorcontrib>Nakajima, Kengo</creatorcontrib><creatorcontrib>Abe, Takaharu</creatorcontrib><creatorcontrib>Yamada, Sakura</creatorcontrib><creatorcontrib>Rikitake, Kodai</creatorcontrib><creatorcontrib>Tanimoto, Kotaro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Oral diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hiraki, Tomoka</au><au>Kunimatsu, Ryo</au><au>Nakajima, Kengo</au><au>Abe, Takaharu</au><au>Yamada, Sakura</au><au>Rikitake, Kodai</au><au>Tanimoto, Kotaro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stem cell‐derived conditioned media from human exfoliated deciduous teeth promote bone regeneration</atitle><jtitle>Oral diseases</jtitle><addtitle>Oral Dis</addtitle><date>2020-03</date><risdate>2020</risdate><volume>26</volume><issue>2</issue><spage>381</spage><epage>390</epage><pages>381-390</pages><issn>1354-523X</issn><eissn>1601-0825</eissn><abstract>Objectives
Cleft lip and palate (CL/P) are common congenital orofacial anomalies. Autogenous iliac bone grafting closes alveolar cleft defects but requires surgical intervention. Mesenchymal stem cell culture supernatant can regenerate tissues via paracrine activity. However, little is known about the bone‐regenerative effects of stem cells from human exfoliated deciduous teeth (SHED) and conditioned media (CM). Our aim was to address this.
Materials and methods
Stem cells were isolated from primary tooth pulp and cultured. Defects were made in calvariae of immunodeficient mice and implanted with stem cell‐ or CM‐containing atelocollagen. Regenerated bone was analysed by microcomputed tomography, haematoxylin–eosin and Masson's trichrome staining. Vascular endothelial growth factor, CD31 and CD34 expression were confirmed by immunohistochemistry, and the presence of several proteins and growth factors was verified in SHED‐CM.
Results
Bone regeneration was enhanced in defects treated with stem cells and CM compared to that in controls 8 weeks after transplantation. Mature bone formation and angiogenesis were confirmed with CM but not with stem cells or in controls. Secretome analysis using multiple cytokine assays revealed that SHED‐CM contained tissue‐regenerating factors with roles in angiogenesis and osteogenesis.
Conclusion
CM non‐invasively regenerate bone and might be effective to reconstruct alveolar clefts in CL/P patients.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31808229</pmid><doi>10.1111/odi.13244</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-5016-4957</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1354-523X |
| ispartof | Oral diseases, 2020-03, Vol.26 (2), p.381-390 |
| issn | 1354-523X 1601-0825 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2322745582 |
| source | MEDLINE; Wiley Journals |
| subjects | Alveolar bone Angiogenesis Animals Atelocollagen Bone grafts Bone growth bone regeneration Bone Regeneration - drug effects CD34 antigen Cell culture Child Cleft Lip - surgery Cleft lip/palate Cleft Palate - surgery Computed tomography conditioned media Culture Media, Conditioned - pharmacology Defects Dental pulp Dental Pulp - physiology Dentistry exfoliated deciduous teeth Growth factors Humans Immunodeficiency Immunohistochemistry Male Mesenchyme Mice Mice, Inbred BALB C Osteogenesis Osteogenesis - drug effects Paracrine signalling Regeneration Secretome Stem cell transplantation Stem cells Stem Cells - physiology Tooth Exfoliation Tooth, Deciduous - cytology Vascular endothelial growth factor Vascular Endothelial Growth Factor A - metabolism X-Ray Microtomography |
| title | Stem cell‐derived conditioned media from human exfoliated deciduous teeth promote bone regeneration |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T06%3A49%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Stem%20cell%E2%80%90derived%20conditioned%20media%20from%20human%20exfoliated%20deciduous%20teeth%20promote%20bone%20regeneration&rft.jtitle=Oral%20diseases&rft.au=Hiraki,%20Tomoka&rft.date=2020-03&rft.volume=26&rft.issue=2&rft.spage=381&rft.epage=390&rft.pages=381-390&rft.issn=1354-523X&rft.eissn=1601-0825&rft_id=info:doi/10.1111/odi.13244&rft_dat=%3Cproquest_cross%3E2322745582%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2356595789&rft_id=info:pmid/31808229&rfr_iscdi=true |