High-volume culture and quantitative real-time PCR for the detection of Aspergillus in sputum

Sputum culture is an insensitive method for the diagnosis of pulmonary aspergillosis. Growth of the organism allows identification of the causative species and susceptibility testing, both of which can inform treatment choices. The current practice is to culture an aliquot of diluted sputum. We asse...

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Veröffentlicht in:	Clinical microbiology and infection 2020-07, Vol.26 (7), p.935-940
Hauptverfasser:	Vergidis, P., Moore, C.B., Novak-Frazer, L., Rautemaa-Richardson, R., Walker, A., Denning, D.W., Richardson, M.D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Allergic bronchopulmonary aspergillosis Antifungal Agents - pharmacology Antifungal Agents - therapeutic use Antifungal resistance Aspergillosis - drug therapy Aspergillosis - microbiology Aspergillosis, Allergic Bronchopulmonary - drug therapy Aspergillosis, Allergic Bronchopulmonary - microbiology Aspergillus - classification Aspergillus - genetics Aspergillus - growth & development Aspergillus - isolation & purification Aspergillus bronchitis Aspergillus fumigatus Bronchitis - drug therapy Bronchitis - microbiology Chronic pulmonary aspergillosis Fungal culture Humans Mycological Typing Techniques PCR Pulmonary Aspergillosis - drug therapy Pulmonary Aspergillosis - microbiology Real-Time Polymerase Chain Reaction Sensitivity and Specificity Sputum - microbiology Treatment Outcome
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