Silencing arginine kinase/integrin β1 subunit by transgenic plant expressing dsRNA inhibits the development and survival of Plutella xylostella
Background Plutella xylostella is a devastating agricultural insect pest of cruciferous plants, including crops. Plant‐mediated RNA interference (RNAi) is currently being developed for plant protection. In this study, we investigated the response of P. xylostella exposed to transgenic Arabidopsis th...
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| Veröffentlicht in: | Pest management science 2020-05, Vol.76 (5), p.1761-1771 |
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| container_title | Pest management science |
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| creator | Fu, Shu Liu, Zhaoxia Chen, Jinzhi Sun, Gengxiao Jiang, Yingxia Li, Miaowen Xiong, Lei Chen, Shaoping Zhou, Yuqing Asad, Muhammad Yang, Guang |
| description | Background
Plutella xylostella is a devastating agricultural insect pest of cruciferous plants, including crops. Plant‐mediated RNA interference (RNAi) is currently being developed for plant protection. In this study, we investigated the response of P. xylostella exposed to transgenic Arabidopsis thaliana plants that expressed double‐stranded RNA (dsRNA) targeting P. xylostella genes of arginine kinase (PxAK) and integrin β1 subunit (Pxβ).
RESULTS
Transgenic plants producing dsRNAs of the 384‐bp fragment of PxAK (dsAK plants), the 497‐bp fragment of Pxβ (dsβ plants), and the 881 bp of the combination of both genes (dsAK‐β plants) were generated and verified. Insect bioassay with these transgenic plants showed that the development of P. xylostella was affected, causing longer developmental time, and lower pupal weight and pupation rate. P. xylostella mortality rates were 25.0% when exposed to dsAK plants, 22.5% with dsβ plants, and 30.0% with dsAK‐β plants, which were all higher than 7.5% for the wild‐type plant. PxAK and Pxβ in P. xylostella were suppressed by 26.6–79.7% at the transcription level by the transgenic plants.
CONCLUSION
These results suggest that plant‐mediated RNAi targeting single gene or both PxAK and Pxβ may have the potential to control P. xylostella. © 2019 Society of Chemical Industry
The present work suggests that plant‐mediated RNAi targeting either or both arginine kinase and integrin β1 subunit genes can have the potential to control pest Plutella xylostella. |
| doi_str_mv | 10.1002/ps.5701 |
| format | Article |
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Plutella xylostella is a devastating agricultural insect pest of cruciferous plants, including crops. Plant‐mediated RNA interference (RNAi) is currently being developed for plant protection. In this study, we investigated the response of P. xylostella exposed to transgenic Arabidopsis thaliana plants that expressed double‐stranded RNA (dsRNA) targeting P. xylostella genes of arginine kinase (PxAK) and integrin β1 subunit (Pxβ).
RESULTS
Transgenic plants producing dsRNAs of the 384‐bp fragment of PxAK (dsAK plants), the 497‐bp fragment of Pxβ (dsβ plants), and the 881 bp of the combination of both genes (dsAK‐β plants) were generated and verified. Insect bioassay with these transgenic plants showed that the development of P. xylostella was affected, causing longer developmental time, and lower pupal weight and pupation rate. P. xylostella mortality rates were 25.0% when exposed to dsAK plants, 22.5% with dsβ plants, and 30.0% with dsAK‐β plants, which were all higher than 7.5% for the wild‐type plant. PxAK and Pxβ in P. xylostella were suppressed by 26.6–79.7% at the transcription level by the transgenic plants.
CONCLUSION
These results suggest that plant‐mediated RNAi targeting single gene or both PxAK and Pxβ may have the potential to control P. xylostella. © 2019 Society of Chemical Industry
The present work suggests that plant‐mediated RNAi targeting either or both arginine kinase and integrin β1 subunit genes can have the potential to control pest Plutella xylostella.</description><identifier>ISSN: 1526-498X</identifier><identifier>EISSN: 1526-4998</identifier><identifier>DOI: 10.1002/ps.5701</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Arabidopsis thaliana ; Arginine ; Arginine kinase ; Bioassays ; Double-stranded RNA ; Gene expression ; Genes ; Insects ; Kinases ; pest control ; Plant protection ; plant‐mediated RNAi ; Plutella xylostella ; Pupation ; Ribonucleic acid ; RNA ; RNA-mediated interference ; Transcription ; Transgenic plants ; β1 integrin</subject><ispartof>Pest management science, 2020-05, Vol.76 (5), p.1761-1771</ispartof><rights>2019 Society of Chemical Industry</rights><rights>Copyright © 2020 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0002-3250-5228</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fps.5701$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fps.5701$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids></links><search><creatorcontrib>Fu, Shu</creatorcontrib><creatorcontrib>Liu, Zhaoxia</creatorcontrib><creatorcontrib>Chen, Jinzhi</creatorcontrib><creatorcontrib>Sun, Gengxiao</creatorcontrib><creatorcontrib>Jiang, Yingxia</creatorcontrib><creatorcontrib>Li, Miaowen</creatorcontrib><creatorcontrib>Xiong, Lei</creatorcontrib><creatorcontrib>Chen, Shaoping</creatorcontrib><creatorcontrib>Zhou, Yuqing</creatorcontrib><creatorcontrib>Asad, Muhammad</creatorcontrib><creatorcontrib>Yang, Guang</creatorcontrib><title>Silencing arginine kinase/integrin β1 subunit by transgenic plant expressing dsRNA inhibits the development and survival of Plutella xylostella</title><title>Pest management science</title><description>Background
Plutella xylostella is a devastating agricultural insect pest of cruciferous plants, including crops. Plant‐mediated RNA interference (RNAi) is currently being developed for plant protection. In this study, we investigated the response of P. xylostella exposed to transgenic Arabidopsis thaliana plants that expressed double‐stranded RNA (dsRNA) targeting P. xylostella genes of arginine kinase (PxAK) and integrin β1 subunit (Pxβ).
RESULTS
Transgenic plants producing dsRNAs of the 384‐bp fragment of PxAK (dsAK plants), the 497‐bp fragment of Pxβ (dsβ plants), and the 881 bp of the combination of both genes (dsAK‐β plants) were generated and verified. Insect bioassay with these transgenic plants showed that the development of P. xylostella was affected, causing longer developmental time, and lower pupal weight and pupation rate. P. xylostella mortality rates were 25.0% when exposed to dsAK plants, 22.5% with dsβ plants, and 30.0% with dsAK‐β plants, which were all higher than 7.5% for the wild‐type plant. PxAK and Pxβ in P. xylostella were suppressed by 26.6–79.7% at the transcription level by the transgenic plants.
CONCLUSION
These results suggest that plant‐mediated RNAi targeting single gene or both PxAK and Pxβ may have the potential to control P. xylostella. © 2019 Society of Chemical Industry
The present work suggests that plant‐mediated RNAi targeting either or both arginine kinase and integrin β1 subunit genes can have the potential to control pest Plutella xylostella.</description><subject>Arabidopsis thaliana</subject><subject>Arginine</subject><subject>Arginine kinase</subject><subject>Bioassays</subject><subject>Double-stranded RNA</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Insects</subject><subject>Kinases</subject><subject>pest control</subject><subject>Plant protection</subject><subject>plant‐mediated RNAi</subject><subject>Plutella xylostella</subject><subject>Pupation</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA-mediated interference</subject><subject>Transcription</subject><subject>Transgenic plants</subject><subject>β1 integrin</subject><issn>1526-498X</issn><issn>1526-4998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNpdkd9KwzAYxYsoOKf4CgFvBOmWpGnTXA7xHwwdTsG7krZft8wsrUk717fwWXwQn8nWiRdenXPx43yH73jeKcEjgjEdV24Uckz2vAEJaeQzIeL9Px-_HHpHzq0wxkIIOvA-5kqDyZRZIGkXyigD6FUZ6WCsTA0Lqwz6-iTINWljVI3SFtVWGrcAozJUaWlqBNvKgnN9Ru4e7ydImaVKVe1QvQSUwwZ0Wa2hI6XJuyS7URupUVmgmW5q0FqibatL92OPvYNCagcnvzr0nq-vni5v_enDzd3lZOpXJOLEz_OCFwIoS7kUnIlUZIwBDjmnYRrTnImIcF7QiDNMI8xoDhHNSSzjqAgKwYKhd77LrWz51oCrk7VyWd_AQNm4hAYUBzELo7BDz_6hq7KxpmvXUTEXhHW3OupiR713H22Tyqq1tG1CcNLPklQu6WdJZvNegm844YPb</recordid><startdate>202005</startdate><enddate>202005</enddate><creator>Fu, Shu</creator><creator>Liu, Zhaoxia</creator><creator>Chen, Jinzhi</creator><creator>Sun, Gengxiao</creator><creator>Jiang, Yingxia</creator><creator>Li, Miaowen</creator><creator>Xiong, Lei</creator><creator>Chen, Shaoping</creator><creator>Zhou, Yuqing</creator><creator>Asad, Muhammad</creator><creator>Yang, Guang</creator><general>John Wiley & Sons, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>7QR</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3250-5228</orcidid></search><sort><creationdate>202005</creationdate><title>Silencing arginine kinase/integrin β1 subunit by transgenic plant expressing dsRNA inhibits the development and survival of Plutella xylostella</title><author>Fu, Shu ; Liu, Zhaoxia ; Chen, Jinzhi ; Sun, Gengxiao ; Jiang, Yingxia ; Li, Miaowen ; Xiong, Lei ; Chen, Shaoping ; Zhou, Yuqing ; Asad, Muhammad ; Yang, Guang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p1671-ddf7f9e24b7a9749b9c44e057725b82d496177f2674026042de62d18a86f3f943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Arabidopsis thaliana</topic><topic>Arginine</topic><topic>Arginine kinase</topic><topic>Bioassays</topic><topic>Double-stranded RNA</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Insects</topic><topic>Kinases</topic><topic>pest control</topic><topic>Plant protection</topic><topic>plant‐mediated RNAi</topic><topic>Plutella xylostella</topic><topic>Pupation</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA-mediated interference</topic><topic>Transcription</topic><topic>Transgenic plants</topic><topic>β1 integrin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fu, Shu</creatorcontrib><creatorcontrib>Liu, Zhaoxia</creatorcontrib><creatorcontrib>Chen, Jinzhi</creatorcontrib><creatorcontrib>Sun, Gengxiao</creatorcontrib><creatorcontrib>Jiang, Yingxia</creatorcontrib><creatorcontrib>Li, Miaowen</creatorcontrib><creatorcontrib>Xiong, Lei</creatorcontrib><creatorcontrib>Chen, Shaoping</creatorcontrib><creatorcontrib>Zhou, Yuqing</creatorcontrib><creatorcontrib>Asad, Muhammad</creatorcontrib><creatorcontrib>Yang, Guang</creatorcontrib><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Pest management science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fu, Shu</au><au>Liu, Zhaoxia</au><au>Chen, Jinzhi</au><au>Sun, Gengxiao</au><au>Jiang, Yingxia</au><au>Li, Miaowen</au><au>Xiong, Lei</au><au>Chen, Shaoping</au><au>Zhou, Yuqing</au><au>Asad, Muhammad</au><au>Yang, Guang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Silencing arginine kinase/integrin β1 subunit by transgenic plant expressing dsRNA inhibits the development and survival of Plutella xylostella</atitle><jtitle>Pest management science</jtitle><date>2020-05</date><risdate>2020</risdate><volume>76</volume><issue>5</issue><spage>1761</spage><epage>1771</epage><pages>1761-1771</pages><issn>1526-498X</issn><eissn>1526-4998</eissn><abstract>Background
Plutella xylostella is a devastating agricultural insect pest of cruciferous plants, including crops. Plant‐mediated RNA interference (RNAi) is currently being developed for plant protection. In this study, we investigated the response of P. xylostella exposed to transgenic Arabidopsis thaliana plants that expressed double‐stranded RNA (dsRNA) targeting P. xylostella genes of arginine kinase (PxAK) and integrin β1 subunit (Pxβ).
RESULTS
Transgenic plants producing dsRNAs of the 384‐bp fragment of PxAK (dsAK plants), the 497‐bp fragment of Pxβ (dsβ plants), and the 881 bp of the combination of both genes (dsAK‐β plants) were generated and verified. Insect bioassay with these transgenic plants showed that the development of P. xylostella was affected, causing longer developmental time, and lower pupal weight and pupation rate. P. xylostella mortality rates were 25.0% when exposed to dsAK plants, 22.5% with dsβ plants, and 30.0% with dsAK‐β plants, which were all higher than 7.5% for the wild‐type plant. PxAK and Pxβ in P. xylostella were suppressed by 26.6–79.7% at the transcription level by the transgenic plants.
CONCLUSION
These results suggest that plant‐mediated RNAi targeting single gene or both PxAK and Pxβ may have the potential to control P. xylostella. © 2019 Society of Chemical Industry
The present work suggests that plant‐mediated RNAi targeting either or both arginine kinase and integrin β1 subunit genes can have the potential to control pest Plutella xylostella.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><doi>10.1002/ps.5701</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-3250-5228</orcidid></addata></record> |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Arabidopsis thaliana Arginine Arginine kinase Bioassays Double-stranded RNA Gene expression Genes Insects Kinases pest control Plant protection plant‐mediated RNAi Plutella xylostella Pupation Ribonucleic acid RNA RNA-mediated interference Transcription Transgenic plants β1 integrin |
| title | Silencing arginine kinase/integrin β1 subunit by transgenic plant expressing dsRNA inhibits the development and survival of Plutella xylostella |
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