Protective effects of a hydrogen-rich solution during cold ischemia in rat lung transplantation
Molecular hydrogen can reduce the oxidative stress of ischemia-reperfusion injury in various organs for transplantation and potentially improve survival rates in recipients. This study aimed to evaluate the protective effects of a hydrogen-rich preservation solution against ischemia-reperfusion inju...
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| Veröffentlicht in: | The Journal of thoracic and cardiovascular surgery 2020-05, Vol.159 (5), p.2110-2118 |
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| container_title | The Journal of thoracic and cardiovascular surgery |
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| creator | Saito, Masao Chen-Yoshikawa, Toyofumi F. Takahashi, Mamoru Kayawake, Hidenao Yokoyama, Yuhei Kurokawa, Ryosuke Hirano, Shin-ichi Date, Hiroshi |
| description | Molecular hydrogen can reduce the oxidative stress of ischemia-reperfusion injury in various organs for transplantation and potentially improve survival rates in recipients. This study aimed to evaluate the protective effects of a hydrogen-rich preservation solution against ischemia-reperfusion injury after cold ischemia in rat lung transplantation.
Lewis rats were divided into a nontransplant group (n = 3), minimum-ischemia group (n = 3), cold ischemia group (n = 6), and cold ischemia with hydrogen-rich (more than 1.0 ppm) preservation solution group (n = 6). The rats in the nontransplant group underwent simple thoracotomy, and the rats in the remaining 3 groups underwent orthotopic left lung transplantation. The ischemic time was |
| doi_str_mv | 10.1016/j.jtcvs.2019.09.175 |
| format | Article |
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Lewis rats were divided into a nontransplant group (n = 3), minimum-ischemia group (n = 3), cold ischemia group (n = 6), and cold ischemia with hydrogen-rich (more than 1.0 ppm) preservation solution group (n = 6). The rats in the nontransplant group underwent simple thoracotomy, and the rats in the remaining 3 groups underwent orthotopic left lung transplantation. The ischemic time was <30 minutes in the minimum-ischemia group and 6 hours in the cold ischemia groups. After 2-hour reperfusion, we evaluated arterial blood gas levels, pulmonary function, lung wet-to-dry weight ratio, and histologic features of the lung tissue. The expression of proinflammatory cytokines was measured using quantitative polymerase chain reaction assays, and 8-hydroxydeoxyguanosine levels were evaluated using enzyme-linked immunosorbent assays.
When compared with the nontransplant and minimum-ischemia groups, the cold ischemia group had lower dynamic compliance, lower oxygenation levels, and higher wet-to-dry weight ratios. However, these variables were significantly improved in the cold ischemia with hydrogen-rich preservation solution group. This group also had fewer signs of perivascular edema, lower interleukin-1β messenger RNA expression, and lower 8-hydroxydeoxyguanosine levels than the cold ischemia group.
The use of a hydrogen-rich preservation solution attenuates ischemia-reperfusion injury in rat lungs during cold ischemia through antioxidant and anti-inflammatory effects.
A hydrogen-rich preservation solution during cold ischemia (ISCH) attenuated lung ischemia-reperfusion injury in rat lung transplantation through inhibition of perivascular edema and alveolar hemorrhage. The hydrogen group had significantly better oxygenation levels and lower 8-hydroxydeoxyguanosine (8-OHdG) levels. ISCH/H2, Cold ischemia with hydrogen-rich preservation solution group; pO2, partial pressure of oxygen. [Display omitted]</description><identifier>ISSN: 0022-5223</identifier><identifier>EISSN: 1097-685X</identifier><identifier>DOI: 10.1016/j.jtcvs.2019.09.175</identifier><identifier>PMID: 31780065</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>hydrogen-rich solution ; ischemia-reperfusion injury ; lung transplantation</subject><ispartof>The Journal of thoracic and cardiovascular surgery, 2020-05, Vol.159 (5), p.2110-2118</ispartof><rights>2019 The American Association for Thoracic Surgery</rights><rights>Copyright © 2019 The American Association for Thoracic Surgery. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-7e1839c8f8214b35dea5eff0f61ea6544c909574d1611a017902cc40e6c493103</citedby><cites>FETCH-LOGICAL-c470t-7e1839c8f8214b35dea5eff0f61ea6544c909574d1611a017902cc40e6c493103</cites><orcidid>0000-0001-8473-3989</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0022522319323554$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31780065$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saito, Masao</creatorcontrib><creatorcontrib>Chen-Yoshikawa, Toyofumi F.</creatorcontrib><creatorcontrib>Takahashi, Mamoru</creatorcontrib><creatorcontrib>Kayawake, Hidenao</creatorcontrib><creatorcontrib>Yokoyama, Yuhei</creatorcontrib><creatorcontrib>Kurokawa, Ryosuke</creatorcontrib><creatorcontrib>Hirano, Shin-ichi</creatorcontrib><creatorcontrib>Date, Hiroshi</creatorcontrib><title>Protective effects of a hydrogen-rich solution during cold ischemia in rat lung transplantation</title><title>The Journal of thoracic and cardiovascular surgery</title><addtitle>J Thorac Cardiovasc Surg</addtitle><description>Molecular hydrogen can reduce the oxidative stress of ischemia-reperfusion injury in various organs for transplantation and potentially improve survival rates in recipients. This study aimed to evaluate the protective effects of a hydrogen-rich preservation solution against ischemia-reperfusion injury after cold ischemia in rat lung transplantation.
Lewis rats were divided into a nontransplant group (n = 3), minimum-ischemia group (n = 3), cold ischemia group (n = 6), and cold ischemia with hydrogen-rich (more than 1.0 ppm) preservation solution group (n = 6). The rats in the nontransplant group underwent simple thoracotomy, and the rats in the remaining 3 groups underwent orthotopic left lung transplantation. The ischemic time was <30 minutes in the minimum-ischemia group and 6 hours in the cold ischemia groups. After 2-hour reperfusion, we evaluated arterial blood gas levels, pulmonary function, lung wet-to-dry weight ratio, and histologic features of the lung tissue. The expression of proinflammatory cytokines was measured using quantitative polymerase chain reaction assays, and 8-hydroxydeoxyguanosine levels were evaluated using enzyme-linked immunosorbent assays.
When compared with the nontransplant and minimum-ischemia groups, the cold ischemia group had lower dynamic compliance, lower oxygenation levels, and higher wet-to-dry weight ratios. However, these variables were significantly improved in the cold ischemia with hydrogen-rich preservation solution group. This group also had fewer signs of perivascular edema, lower interleukin-1β messenger RNA expression, and lower 8-hydroxydeoxyguanosine levels than the cold ischemia group.
The use of a hydrogen-rich preservation solution attenuates ischemia-reperfusion injury in rat lungs during cold ischemia through antioxidant and anti-inflammatory effects.
A hydrogen-rich preservation solution during cold ischemia (ISCH) attenuated lung ischemia-reperfusion injury in rat lung transplantation through inhibition of perivascular edema and alveolar hemorrhage. The hydrogen group had significantly better oxygenation levels and lower 8-hydroxydeoxyguanosine (8-OHdG) levels. ISCH/H2, Cold ischemia with hydrogen-rich preservation solution group; pO2, partial pressure of oxygen. [Display omitted]</description><subject>hydrogen-rich solution</subject><subject>ischemia-reperfusion injury</subject><subject>lung transplantation</subject><issn>0022-5223</issn><issn>1097-685X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kEFv2yAYhtHUaUmz_YJJFcde7H6Abcxhh6rq2kqRtsMm7YYI_pwQOSYFHCn_fmTJeuwJJJ6X7_0eQr4yKBmw5m5bbpM9xJIDUyWoksn6A5kzULJo2vrPFZkDcF7UnIsZuY5xCwAys5_ITDDZAjT1nOifwSe0yR2QYt_nW6S-p4Zujl3waxyL4OyGRj9MyfmRdlNw45paP3TURbvBnTPUjTSYRIcpv6RgxrgfzJjMKfCZfOzNEPHL5VyQ398ffz08F8sfTy8P98vCVhJSIZG1Qtm2bzmrVqLu0NS5DvQNQ9PUVWUVqFpWHWsYM8CkAm5tBdjYSgkGYkFuz__ug3-dMCa9y_VwyEXQT1FzwUG0XEqVUXFGbfAxBuz1PridCUfNQJ_M6q3-Z1afzGpQOpvNqZvLgGm1w-4t819lBr6dAcxrHhwGHa3D0WLnQtaqO-_eHfAXPaGLzg</recordid><startdate>202005</startdate><enddate>202005</enddate><creator>Saito, Masao</creator><creator>Chen-Yoshikawa, Toyofumi F.</creator><creator>Takahashi, Mamoru</creator><creator>Kayawake, Hidenao</creator><creator>Yokoyama, Yuhei</creator><creator>Kurokawa, Ryosuke</creator><creator>Hirano, Shin-ichi</creator><creator>Date, Hiroshi</creator><general>Elsevier Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8473-3989</orcidid></search><sort><creationdate>202005</creationdate><title>Protective effects of a hydrogen-rich solution during cold ischemia in rat lung transplantation</title><author>Saito, Masao ; Chen-Yoshikawa, Toyofumi F. ; Takahashi, Mamoru ; Kayawake, Hidenao ; Yokoyama, Yuhei ; Kurokawa, Ryosuke ; Hirano, Shin-ichi ; Date, Hiroshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-7e1839c8f8214b35dea5eff0f61ea6544c909574d1611a017902cc40e6c493103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>hydrogen-rich solution</topic><topic>ischemia-reperfusion injury</topic><topic>lung transplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saito, Masao</creatorcontrib><creatorcontrib>Chen-Yoshikawa, Toyofumi F.</creatorcontrib><creatorcontrib>Takahashi, Mamoru</creatorcontrib><creatorcontrib>Kayawake, Hidenao</creatorcontrib><creatorcontrib>Yokoyama, Yuhei</creatorcontrib><creatorcontrib>Kurokawa, Ryosuke</creatorcontrib><creatorcontrib>Hirano, Shin-ichi</creatorcontrib><creatorcontrib>Date, Hiroshi</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of thoracic and cardiovascular surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saito, Masao</au><au>Chen-Yoshikawa, Toyofumi F.</au><au>Takahashi, Mamoru</au><au>Kayawake, Hidenao</au><au>Yokoyama, Yuhei</au><au>Kurokawa, Ryosuke</au><au>Hirano, Shin-ichi</au><au>Date, Hiroshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective effects of a hydrogen-rich solution during cold ischemia in rat lung transplantation</atitle><jtitle>The Journal of thoracic and cardiovascular surgery</jtitle><addtitle>J Thorac Cardiovasc Surg</addtitle><date>2020-05</date><risdate>2020</risdate><volume>159</volume><issue>5</issue><spage>2110</spage><epage>2118</epage><pages>2110-2118</pages><issn>0022-5223</issn><eissn>1097-685X</eissn><abstract>Molecular hydrogen can reduce the oxidative stress of ischemia-reperfusion injury in various organs for transplantation and potentially improve survival rates in recipients. This study aimed to evaluate the protective effects of a hydrogen-rich preservation solution against ischemia-reperfusion injury after cold ischemia in rat lung transplantation.
Lewis rats were divided into a nontransplant group (n = 3), minimum-ischemia group (n = 3), cold ischemia group (n = 6), and cold ischemia with hydrogen-rich (more than 1.0 ppm) preservation solution group (n = 6). The rats in the nontransplant group underwent simple thoracotomy, and the rats in the remaining 3 groups underwent orthotopic left lung transplantation. The ischemic time was <30 minutes in the minimum-ischemia group and 6 hours in the cold ischemia groups. After 2-hour reperfusion, we evaluated arterial blood gas levels, pulmonary function, lung wet-to-dry weight ratio, and histologic features of the lung tissue. The expression of proinflammatory cytokines was measured using quantitative polymerase chain reaction assays, and 8-hydroxydeoxyguanosine levels were evaluated using enzyme-linked immunosorbent assays.
When compared with the nontransplant and minimum-ischemia groups, the cold ischemia group had lower dynamic compliance, lower oxygenation levels, and higher wet-to-dry weight ratios. However, these variables were significantly improved in the cold ischemia with hydrogen-rich preservation solution group. This group also had fewer signs of perivascular edema, lower interleukin-1β messenger RNA expression, and lower 8-hydroxydeoxyguanosine levels than the cold ischemia group.
The use of a hydrogen-rich preservation solution attenuates ischemia-reperfusion injury in rat lungs during cold ischemia through antioxidant and anti-inflammatory effects.
A hydrogen-rich preservation solution during cold ischemia (ISCH) attenuated lung ischemia-reperfusion injury in rat lung transplantation through inhibition of perivascular edema and alveolar hemorrhage. The hydrogen group had significantly better oxygenation levels and lower 8-hydroxydeoxyguanosine (8-OHdG) levels. ISCH/H2, Cold ischemia with hydrogen-rich preservation solution group; pO2, partial pressure of oxygen. [Display omitted]</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>31780065</pmid><doi>10.1016/j.jtcvs.2019.09.175</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-8473-3989</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | hydrogen-rich solution ischemia-reperfusion injury lung transplantation |
| title | Protective effects of a hydrogen-rich solution during cold ischemia in rat lung transplantation |
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