Plasma signature of apoptotic microvesicles is associated with endothelial dysfunction and plaque rupture in acute coronary syndromes
Circulating microvesicles (MV) are surrogate biomarkers of atherosclerosis. However, their role in acute coronary syndromes (ACS) has not been fully elucidated yet. We sought to examine the association of circulating apoptotic MVs with ACS pathophysiology. One hundred and fifty-three patients (n = 1...
Gespeichert in:
| Veröffentlicht in: | Journal of molecular and cellular cardiology 2020-01, Vol.138, p.110-114 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 114 |
|---|---|
| container_issue | |
| container_start_page | 110 |
| container_title | Journal of molecular and cellular cardiology |
| container_volume | 138 |
| creator | Zacharia, Effimia Antonopoulos, Alexios S. Oikonomou, Evangelos Papageorgiou, Nikolaos Pallantza, Zoi Miliou, Antigoni Mystakidi, Vasiliki Chara Simantiris, Spyridon Kriebardis, Anastasios Orologas, Nikolaos Valasiadi, Eftychia Papaioannou, Spyridon Galiatsatos, Nikolaos Antoniades, Charalambos Tousoulis, Dimitris |
| description | Circulating microvesicles (MV) are surrogate biomarkers of atherosclerosis. However, their role in acute coronary syndromes (ACS) has not been fully elucidated yet. We sought to examine the association of circulating apoptotic MVs with ACS pathophysiology.
One hundred and fifty-three patients (n = 153) were included in the study; 49 patients with ST-elevation myocardial infarction (STEMI), 35 with non-STEMI (NSTEMI), 38 with unstable angina, 15 with stable coronary artery disease and 16 control individuals. Flow cytometry analysis was used to quantify circulating apoptotic/non-apoptotic (phospatidyloserine+/phospatidyloserine−) endothelial cell (EMV), red blood cell (RMV) and platelet (PMV) derived MV. Flow-mediated dilatation (FMD) of the brachial artery was assessed by ultrasound to estimate endothelial function. The inflammatory profile was assessed by serum C-reactive protein (hsCRP) levels. Apoptotic only (but not non-apoptotic) MV were increased in patients with ACS (EMV, P = 2.32 × 10−9; RMV, P = .0019; PMV, P = .01). Hierarchical clustering of the total population of ACS patients (n = 122) by circulating levels of phospatidyloserine+ EMV, RMV and PMV identified two discreet clusters of patients without any differences in traditional risk factors, but significant differences in brachial FMD (5.2% (2.5) vs. 4.1% (2.3), P |
| doi_str_mv | 10.1016/j.yjmcc.2019.11.153 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2320378673</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0022282819303803</els_id><sourcerecordid>2320378673</sourcerecordid><originalsourceid>FETCH-LOGICAL-c359t-e05b8bb66179e0a45aea65ce5d160cb26363feca5839a37541c38d92c21f3abc3</originalsourceid><addsrcrecordid>eNp9kEtv1DAQxy0EokvhEyAhH7lksT3rPA4cUMVLqgQHOFvOeEK9SuLgcYr2A_C9SbuFI6eRRv_HzE-Il1rttdL1m-P-dJwQ90bpbq_1Xlt4JHZadbZqbXt4LHZKGVOZ1rQX4hnzUSnVHQCeigvQTQsKYCd-fx09T15y_DH7smaSaZB-SUtJJaKcIuZ0SxxxJJaRpWdOGH2hIH_FciNpDqnc0Bj9KMOJh3XGEtMs_RzkMvqfK8m8LvfBcdviWkhiymn2-ST5NIecJuLn4sngR6YXD_NSfP_w_tvVp-r6y8fPV--uKwTblYqU7du-r2vddKT8wXrytUWyQdcKe1NDDQOhty10Hhp70Aht6AwaPYDvES7F63PuktN2Ghc3RUYaRz9TWtkZMAqatm5gk8JZugFgzjS4Jcdpu9pp5e74u6O75-_u-Dut3cZ_c716KFj7icI_z1_gm-DtWUDbm7eRsmOMNCOFmAmLCyn-t-APpxWb1w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2320378673</pqid></control><display><type>article</type><title>Plasma signature of apoptotic microvesicles is associated with endothelial dysfunction and plaque rupture in acute coronary syndromes</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Zacharia, Effimia ; Antonopoulos, Alexios S. ; Oikonomou, Evangelos ; Papageorgiou, Nikolaos ; Pallantza, Zoi ; Miliou, Antigoni ; Mystakidi, Vasiliki Chara ; Simantiris, Spyridon ; Kriebardis, Anastasios ; Orologas, Nikolaos ; Valasiadi, Eftychia ; Papaioannou, Spyridon ; Galiatsatos, Nikolaos ; Antoniades, Charalambos ; Tousoulis, Dimitris</creator><creatorcontrib>Zacharia, Effimia ; Antonopoulos, Alexios S. ; Oikonomou, Evangelos ; Papageorgiou, Nikolaos ; Pallantza, Zoi ; Miliou, Antigoni ; Mystakidi, Vasiliki Chara ; Simantiris, Spyridon ; Kriebardis, Anastasios ; Orologas, Nikolaos ; Valasiadi, Eftychia ; Papaioannou, Spyridon ; Galiatsatos, Nikolaos ; Antoniades, Charalambos ; Tousoulis, Dimitris</creatorcontrib><description>Circulating microvesicles (MV) are surrogate biomarkers of atherosclerosis. However, their role in acute coronary syndromes (ACS) has not been fully elucidated yet. We sought to examine the association of circulating apoptotic MVs with ACS pathophysiology.
One hundred and fifty-three patients (n = 153) were included in the study; 49 patients with ST-elevation myocardial infarction (STEMI), 35 with non-STEMI (NSTEMI), 38 with unstable angina, 15 with stable coronary artery disease and 16 control individuals. Flow cytometry analysis was used to quantify circulating apoptotic/non-apoptotic (phospatidyloserine+/phospatidyloserine−) endothelial cell (EMV), red blood cell (RMV) and platelet (PMV) derived MV. Flow-mediated dilatation (FMD) of the brachial artery was assessed by ultrasound to estimate endothelial function. The inflammatory profile was assessed by serum C-reactive protein (hsCRP) levels. Apoptotic only (but not non-apoptotic) MV were increased in patients with ACS (EMV, P = 2.32 × 10−9; RMV, P = .0019; PMV, P = .01). Hierarchical clustering of the total population of ACS patients (n = 122) by circulating levels of phospatidyloserine+ EMV, RMV and PMV identified two discreet clusters of patients without any differences in traditional risk factors, but significant differences in brachial FMD (5.2% (2.5) vs. 4.1% (2.3), P < .05) that remained significant after adjustment for co-variates. The prevalence of STEMI, a surrogate for plaque rupture and vessel thrombotic occlusion, was significantly higher in the patient cluster with impaired endothelial function (60% vs 32%, P = .016, adjusted odds ratio for STEMI, 3.041, 95%CI, 1.160 to 7.968, p = .024).
Our findings indicate that the circulating levels of apoptotic MV are increased in ACS patients and their plasma profiles associate with endothelial dysfunction and thrombotic complications in ACS patients.
•Plasma levels of apoptotic cell-derived microvesicles are significantly increased in patients with acute coronary syndromes•A distinct signature of plasma apoptotic microvesicles is related to worse endothelial function in patients with ACS.•This distinct signature of microvesicles is also related to higher OR for STEMI in the absence of differences in risk profile.•These findings provide evidence on the role of plasma microvesicles in the pathophysiology of ACS and plaque rupture.</description><identifier>ISSN: 0022-2828</identifier><identifier>EISSN: 1095-8584</identifier><identifier>DOI: 10.1016/j.yjmcc.2019.11.153</identifier><identifier>PMID: 31783033</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Acute Coronary Syndrome - blood ; Acute Coronary Syndrome - physiopathology ; Acute coronary syndromes ; Apoptosis ; Apoptotic ; Cell-Derived Microparticles - metabolism ; Endothelial dysfunction ; Endothelium, Vascular - pathology ; Endothelium, Vascular - physiopathology ; Female ; Humans ; Male ; Microvesicles ; Middle Aged ; Plaque rupture ; Plaque, Atherosclerotic - blood ; Plaque, Atherosclerotic - physiopathology ; Rupture</subject><ispartof>Journal of molecular and cellular cardiology, 2020-01, Vol.138, p.110-114</ispartof><rights>2019 Elsevier Ltd</rights><rights>Copyright © 2019 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-e05b8bb66179e0a45aea65ce5d160cb26363feca5839a37541c38d92c21f3abc3</citedby><cites>FETCH-LOGICAL-c359t-e05b8bb66179e0a45aea65ce5d160cb26363feca5839a37541c38d92c21f3abc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.yjmcc.2019.11.153$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31783033$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zacharia, Effimia</creatorcontrib><creatorcontrib>Antonopoulos, Alexios S.</creatorcontrib><creatorcontrib>Oikonomou, Evangelos</creatorcontrib><creatorcontrib>Papageorgiou, Nikolaos</creatorcontrib><creatorcontrib>Pallantza, Zoi</creatorcontrib><creatorcontrib>Miliou, Antigoni</creatorcontrib><creatorcontrib>Mystakidi, Vasiliki Chara</creatorcontrib><creatorcontrib>Simantiris, Spyridon</creatorcontrib><creatorcontrib>Kriebardis, Anastasios</creatorcontrib><creatorcontrib>Orologas, Nikolaos</creatorcontrib><creatorcontrib>Valasiadi, Eftychia</creatorcontrib><creatorcontrib>Papaioannou, Spyridon</creatorcontrib><creatorcontrib>Galiatsatos, Nikolaos</creatorcontrib><creatorcontrib>Antoniades, Charalambos</creatorcontrib><creatorcontrib>Tousoulis, Dimitris</creatorcontrib><title>Plasma signature of apoptotic microvesicles is associated with endothelial dysfunction and plaque rupture in acute coronary syndromes</title><title>Journal of molecular and cellular cardiology</title><addtitle>J Mol Cell Cardiol</addtitle><description>Circulating microvesicles (MV) are surrogate biomarkers of atherosclerosis. However, their role in acute coronary syndromes (ACS) has not been fully elucidated yet. We sought to examine the association of circulating apoptotic MVs with ACS pathophysiology.
One hundred and fifty-three patients (n = 153) were included in the study; 49 patients with ST-elevation myocardial infarction (STEMI), 35 with non-STEMI (NSTEMI), 38 with unstable angina, 15 with stable coronary artery disease and 16 control individuals. Flow cytometry analysis was used to quantify circulating apoptotic/non-apoptotic (phospatidyloserine+/phospatidyloserine−) endothelial cell (EMV), red blood cell (RMV) and platelet (PMV) derived MV. Flow-mediated dilatation (FMD) of the brachial artery was assessed by ultrasound to estimate endothelial function. The inflammatory profile was assessed by serum C-reactive protein (hsCRP) levels. Apoptotic only (but not non-apoptotic) MV were increased in patients with ACS (EMV, P = 2.32 × 10−9; RMV, P = .0019; PMV, P = .01). Hierarchical clustering of the total population of ACS patients (n = 122) by circulating levels of phospatidyloserine+ EMV, RMV and PMV identified two discreet clusters of patients without any differences in traditional risk factors, but significant differences in brachial FMD (5.2% (2.5) vs. 4.1% (2.3), P < .05) that remained significant after adjustment for co-variates. The prevalence of STEMI, a surrogate for plaque rupture and vessel thrombotic occlusion, was significantly higher in the patient cluster with impaired endothelial function (60% vs 32%, P = .016, adjusted odds ratio for STEMI, 3.041, 95%CI, 1.160 to 7.968, p = .024).
Our findings indicate that the circulating levels of apoptotic MV are increased in ACS patients and their plasma profiles associate with endothelial dysfunction and thrombotic complications in ACS patients.
•Plasma levels of apoptotic cell-derived microvesicles are significantly increased in patients with acute coronary syndromes•A distinct signature of plasma apoptotic microvesicles is related to worse endothelial function in patients with ACS.•This distinct signature of microvesicles is also related to higher OR for STEMI in the absence of differences in risk profile.•These findings provide evidence on the role of plasma microvesicles in the pathophysiology of ACS and plaque rupture.</description><subject>Acute Coronary Syndrome - blood</subject><subject>Acute Coronary Syndrome - physiopathology</subject><subject>Acute coronary syndromes</subject><subject>Apoptosis</subject><subject>Apoptotic</subject><subject>Cell-Derived Microparticles - metabolism</subject><subject>Endothelial dysfunction</subject><subject>Endothelium, Vascular - pathology</subject><subject>Endothelium, Vascular - physiopathology</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Microvesicles</subject><subject>Middle Aged</subject><subject>Plaque rupture</subject><subject>Plaque, Atherosclerotic - blood</subject><subject>Plaque, Atherosclerotic - physiopathology</subject><subject>Rupture</subject><issn>0022-2828</issn><issn>1095-8584</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtv1DAQxy0EokvhEyAhH7lksT3rPA4cUMVLqgQHOFvOeEK9SuLgcYr2A_C9SbuFI6eRRv_HzE-Il1rttdL1m-P-dJwQ90bpbq_1Xlt4JHZadbZqbXt4LHZKGVOZ1rQX4hnzUSnVHQCeigvQTQsKYCd-fx09T15y_DH7smaSaZB-SUtJJaKcIuZ0SxxxJJaRpWdOGH2hIH_FciNpDqnc0Bj9KMOJh3XGEtMs_RzkMvqfK8m8LvfBcdviWkhiymn2-ST5NIecJuLn4sngR6YXD_NSfP_w_tvVp-r6y8fPV--uKwTblYqU7du-r2vddKT8wXrytUWyQdcKe1NDDQOhty10Hhp70Aht6AwaPYDvES7F63PuktN2Ghc3RUYaRz9TWtkZMAqatm5gk8JZugFgzjS4Jcdpu9pp5e74u6O75-_u-Dut3cZ_c716KFj7icI_z1_gm-DtWUDbm7eRsmOMNCOFmAmLCyn-t-APpxWb1w</recordid><startdate>202001</startdate><enddate>202001</enddate><creator>Zacharia, Effimia</creator><creator>Antonopoulos, Alexios S.</creator><creator>Oikonomou, Evangelos</creator><creator>Papageorgiou, Nikolaos</creator><creator>Pallantza, Zoi</creator><creator>Miliou, Antigoni</creator><creator>Mystakidi, Vasiliki Chara</creator><creator>Simantiris, Spyridon</creator><creator>Kriebardis, Anastasios</creator><creator>Orologas, Nikolaos</creator><creator>Valasiadi, Eftychia</creator><creator>Papaioannou, Spyridon</creator><creator>Galiatsatos, Nikolaos</creator><creator>Antoniades, Charalambos</creator><creator>Tousoulis, Dimitris</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>202001</creationdate><title>Plasma signature of apoptotic microvesicles is associated with endothelial dysfunction and plaque rupture in acute coronary syndromes</title><author>Zacharia, Effimia ; Antonopoulos, Alexios S. ; Oikonomou, Evangelos ; Papageorgiou, Nikolaos ; Pallantza, Zoi ; Miliou, Antigoni ; Mystakidi, Vasiliki Chara ; Simantiris, Spyridon ; Kriebardis, Anastasios ; Orologas, Nikolaos ; Valasiadi, Eftychia ; Papaioannou, Spyridon ; Galiatsatos, Nikolaos ; Antoniades, Charalambos ; Tousoulis, Dimitris</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-e05b8bb66179e0a45aea65ce5d160cb26363feca5839a37541c38d92c21f3abc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acute Coronary Syndrome - blood</topic><topic>Acute Coronary Syndrome - physiopathology</topic><topic>Acute coronary syndromes</topic><topic>Apoptosis</topic><topic>Apoptotic</topic><topic>Cell-Derived Microparticles - metabolism</topic><topic>Endothelial dysfunction</topic><topic>Endothelium, Vascular - pathology</topic><topic>Endothelium, Vascular - physiopathology</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Microvesicles</topic><topic>Middle Aged</topic><topic>Plaque rupture</topic><topic>Plaque, Atherosclerotic - blood</topic><topic>Plaque, Atherosclerotic - physiopathology</topic><topic>Rupture</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zacharia, Effimia</creatorcontrib><creatorcontrib>Antonopoulos, Alexios S.</creatorcontrib><creatorcontrib>Oikonomou, Evangelos</creatorcontrib><creatorcontrib>Papageorgiou, Nikolaos</creatorcontrib><creatorcontrib>Pallantza, Zoi</creatorcontrib><creatorcontrib>Miliou, Antigoni</creatorcontrib><creatorcontrib>Mystakidi, Vasiliki Chara</creatorcontrib><creatorcontrib>Simantiris, Spyridon</creatorcontrib><creatorcontrib>Kriebardis, Anastasios</creatorcontrib><creatorcontrib>Orologas, Nikolaos</creatorcontrib><creatorcontrib>Valasiadi, Eftychia</creatorcontrib><creatorcontrib>Papaioannou, Spyridon</creatorcontrib><creatorcontrib>Galiatsatos, Nikolaos</creatorcontrib><creatorcontrib>Antoniades, Charalambos</creatorcontrib><creatorcontrib>Tousoulis, Dimitris</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular and cellular cardiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zacharia, Effimia</au><au>Antonopoulos, Alexios S.</au><au>Oikonomou, Evangelos</au><au>Papageorgiou, Nikolaos</au><au>Pallantza, Zoi</au><au>Miliou, Antigoni</au><au>Mystakidi, Vasiliki Chara</au><au>Simantiris, Spyridon</au><au>Kriebardis, Anastasios</au><au>Orologas, Nikolaos</au><au>Valasiadi, Eftychia</au><au>Papaioannou, Spyridon</au><au>Galiatsatos, Nikolaos</au><au>Antoniades, Charalambos</au><au>Tousoulis, Dimitris</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plasma signature of apoptotic microvesicles is associated with endothelial dysfunction and plaque rupture in acute coronary syndromes</atitle><jtitle>Journal of molecular and cellular cardiology</jtitle><addtitle>J Mol Cell Cardiol</addtitle><date>2020-01</date><risdate>2020</risdate><volume>138</volume><spage>110</spage><epage>114</epage><pages>110-114</pages><issn>0022-2828</issn><eissn>1095-8584</eissn><abstract>Circulating microvesicles (MV) are surrogate biomarkers of atherosclerosis. However, their role in acute coronary syndromes (ACS) has not been fully elucidated yet. We sought to examine the association of circulating apoptotic MVs with ACS pathophysiology.
One hundred and fifty-three patients (n = 153) were included in the study; 49 patients with ST-elevation myocardial infarction (STEMI), 35 with non-STEMI (NSTEMI), 38 with unstable angina, 15 with stable coronary artery disease and 16 control individuals. Flow cytometry analysis was used to quantify circulating apoptotic/non-apoptotic (phospatidyloserine+/phospatidyloserine−) endothelial cell (EMV), red blood cell (RMV) and platelet (PMV) derived MV. Flow-mediated dilatation (FMD) of the brachial artery was assessed by ultrasound to estimate endothelial function. The inflammatory profile was assessed by serum C-reactive protein (hsCRP) levels. Apoptotic only (but not non-apoptotic) MV were increased in patients with ACS (EMV, P = 2.32 × 10−9; RMV, P = .0019; PMV, P = .01). Hierarchical clustering of the total population of ACS patients (n = 122) by circulating levels of phospatidyloserine+ EMV, RMV and PMV identified two discreet clusters of patients without any differences in traditional risk factors, but significant differences in brachial FMD (5.2% (2.5) vs. 4.1% (2.3), P < .05) that remained significant after adjustment for co-variates. The prevalence of STEMI, a surrogate for plaque rupture and vessel thrombotic occlusion, was significantly higher in the patient cluster with impaired endothelial function (60% vs 32%, P = .016, adjusted odds ratio for STEMI, 3.041, 95%CI, 1.160 to 7.968, p = .024).
Our findings indicate that the circulating levels of apoptotic MV are increased in ACS patients and their plasma profiles associate with endothelial dysfunction and thrombotic complications in ACS patients.
•Plasma levels of apoptotic cell-derived microvesicles are significantly increased in patients with acute coronary syndromes•A distinct signature of plasma apoptotic microvesicles is related to worse endothelial function in patients with ACS.•This distinct signature of microvesicles is also related to higher OR for STEMI in the absence of differences in risk profile.•These findings provide evidence on the role of plasma microvesicles in the pathophysiology of ACS and plaque rupture.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>31783033</pmid><doi>10.1016/j.yjmcc.2019.11.153</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-2828 |
| ispartof | Journal of molecular and cellular cardiology, 2020-01, Vol.138, p.110-114 |
| issn | 0022-2828 1095-8584 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2320378673 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Acute Coronary Syndrome - blood Acute Coronary Syndrome - physiopathology Acute coronary syndromes Apoptosis Apoptotic Cell-Derived Microparticles - metabolism Endothelial dysfunction Endothelium, Vascular - pathology Endothelium, Vascular - physiopathology Female Humans Male Microvesicles Middle Aged Plaque rupture Plaque, Atherosclerotic - blood Plaque, Atherosclerotic - physiopathology Rupture |
| title | Plasma signature of apoptotic microvesicles is associated with endothelial dysfunction and plaque rupture in acute coronary syndromes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T15%3A12%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Plasma%20signature%20of%20apoptotic%20microvesicles%20is%20associated%20with%20endothelial%20dysfunction%20and%20plaque%20rupture%20in%20acute%20coronary%20syndromes&rft.jtitle=Journal%20of%20molecular%20and%20cellular%20cardiology&rft.au=Zacharia,%20Effimia&rft.date=2020-01&rft.volume=138&rft.spage=110&rft.epage=114&rft.pages=110-114&rft.issn=0022-2828&rft.eissn=1095-8584&rft_id=info:doi/10.1016/j.yjmcc.2019.11.153&rft_dat=%3Cproquest_cross%3E2320378673%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2320378673&rft_id=info:pmid/31783033&rft_els_id=S0022282819303803&rfr_iscdi=true |