Pathogen displacement during intermittent catheter insertion: a novel in vitro urethra model
Aim To develop a novel in vitro urethra model and use it to determine if insertion of an intermittent urinary catheter (IC) displaces pathogenic bacteria from the urethral meatus along the urethra. Methods Displacement of microbial growth after catheter insertion was assessed using a novel in vitro...
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| Veröffentlicht in: | Journal of applied microbiology 2020-04, Vol.128 (4), p.1191-1200 |
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| creator | Cortese, Y.J. Wagner, V.E. Tierney, M. Scully, D. Devine, D.M. Fogarty, A. |
| description | Aim
To develop a novel in vitro urethra model and use it to determine if insertion of an intermittent urinary catheter (IC) displaces pathogenic bacteria from the urethral meatus along the urethra.
Methods
Displacement of microbial growth after catheter insertion was assessed using a novel in vitro urethra model. The in vitro urethra model utilized chromogenic agar and was inoculated with bacteria at one side of the artificial urethra channel, to act as a contaminated urethral meatus, before an IC was inserted into the channel. Three ICs types were used to validate the in vitro urethra model and methodology.
Results
When compared to the bacterial growth control, a significant difference in bacterial growth was found after insertion of the uncoated (P ≤ 0·001) and hydrophilic coated (P ≤ 0·009) catheters; no significant difference when a prototype catheter was inserted into the in vitro urethra model with either bacterial species tested (P ≥ 0·423).
Conclusion
The results presented support the hypothesis that a single catheter insertion can initiate a catheter‐associated urinary tract infection.
Significance and Impact of the Study
The in vitro urethra model and associated methodology were found to be reliable and reproducible (P ≥ 0·265) providing new research tool for the development and validation of emerging technologies in urological healthcare. |
| doi_str_mv | 10.1111/jam.14533 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2320375315</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2369751914</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3533-6f135263a76dde5a5f52ca4159243890652bc19b515982e88467f2ed3ae964b43</originalsourceid><addsrcrecordid>eNp1kE9LwzAYh4Mobk4PfgEpeNFDtyZp0tbbGP5loge9CSVr324ZbTKTdLJvb7ZOD4LvJW9-PDy8_BA6x9EQ-xktRTPEMaP0APUx5SwkPCGHuz0OWZSQHjqxdhlFmEaMH6MexUlKUs776ONVuIWegwpKaVe1KKAB5YKyNVLNA6kcmEY6t80KT4L_-9SCcVKrm0AESq-h9lGwls7ooDXgFkYEjS6hPkVHlagtnO3fAXq_u32bPITTl_vHyXgaFtQfHfIKU0Y4FQkvS2CCVYwUIsYsIzFNs4gzMitwNmM-SQmkacyTikBJBWQ8nsV0gK4678rozxasyxtpC6hroUC3NieURDRhFDOPXv5Bl7o1yl_nKZ4lDGd4K7zuqMJoaw1U-crIRphNjqN8W3nuK893lXv2Ym9sZw2Uv-RPxx4YdcCXrGHzvyl_Gj93ym9b9Ync</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2369751914</pqid></control><display><type>article</type><title>Pathogen displacement during intermittent catheter insertion: a novel in vitro urethra model</title><source>MEDLINE</source><source>Wiley Journals</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Cortese, Y.J. ; Wagner, V.E. ; Tierney, M. ; Scully, D. ; Devine, D.M. ; Fogarty, A.</creator><creatorcontrib>Cortese, Y.J. ; Wagner, V.E. ; Tierney, M. ; Scully, D. ; Devine, D.M. ; Fogarty, A.</creatorcontrib><description>Aim
To develop a novel in vitro urethra model and use it to determine if insertion of an intermittent urinary catheter (IC) displaces pathogenic bacteria from the urethral meatus along the urethra.
Methods
Displacement of microbial growth after catheter insertion was assessed using a novel in vitro urethra model. The in vitro urethra model utilized chromogenic agar and was inoculated with bacteria at one side of the artificial urethra channel, to act as a contaminated urethral meatus, before an IC was inserted into the channel. Three ICs types were used to validate the in vitro urethra model and methodology.
Results
When compared to the bacterial growth control, a significant difference in bacterial growth was found after insertion of the uncoated (P ≤ 0·001) and hydrophilic coated (P ≤ 0·009) catheters; no significant difference when a prototype catheter was inserted into the in vitro urethra model with either bacterial species tested (P ≥ 0·423).
Conclusion
The results presented support the hypothesis that a single catheter insertion can initiate a catheter‐associated urinary tract infection.
Significance and Impact of the Study
The in vitro urethra model and associated methodology were found to be reliable and reproducible (P ≥ 0·265) providing new research tool for the development and validation of emerging technologies in urological healthcare.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.14533</identifier><identifier>PMID: 31782866</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Bacteria ; Bacteria - growth & development ; Bacteria - isolation & purification ; Catheter-Related Infections - microbiology ; Catheters ; Displacement ; E. coli (all potentially pathogenic types) ; Equipment Contamination ; Humans ; In vitro methods and tests ; infection ; Insertion ; Intermittent Urethral Catheterization - adverse effects ; Intermittent Urethral Catheterization - instrumentation ; Male ; mechanism of action ; Medical instruments ; microbial contamination ; Microorganisms ; Models, Biological ; New technology ; staphylococci ; Urethra ; Urethra - microbiology ; Urinary Catheters - microbiology ; Urinary tract ; Urinary tract infections ; Urinary Tract Infections - microbiology</subject><ispartof>Journal of applied microbiology, 2020-04, Vol.128 (4), p.1191-1200</ispartof><rights>2019 The Society for Applied Microbiology</rights><rights>2019 The Society for Applied Microbiology.</rights><rights>Copyright © 2020 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3533-6f135263a76dde5a5f52ca4159243890652bc19b515982e88467f2ed3ae964b43</citedby><cites>FETCH-LOGICAL-c3533-6f135263a76dde5a5f52ca4159243890652bc19b515982e88467f2ed3ae964b43</cites><orcidid>0000-0002-1364-5583 ; 0000-0001-9812-480X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.14533$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.14533$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31782866$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cortese, Y.J.</creatorcontrib><creatorcontrib>Wagner, V.E.</creatorcontrib><creatorcontrib>Tierney, M.</creatorcontrib><creatorcontrib>Scully, D.</creatorcontrib><creatorcontrib>Devine, D.M.</creatorcontrib><creatorcontrib>Fogarty, A.</creatorcontrib><title>Pathogen displacement during intermittent catheter insertion: a novel in vitro urethra model</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aim
To develop a novel in vitro urethra model and use it to determine if insertion of an intermittent urinary catheter (IC) displaces pathogenic bacteria from the urethral meatus along the urethra.
Methods
Displacement of microbial growth after catheter insertion was assessed using a novel in vitro urethra model. The in vitro urethra model utilized chromogenic agar and was inoculated with bacteria at one side of the artificial urethra channel, to act as a contaminated urethral meatus, before an IC was inserted into the channel. Three ICs types were used to validate the in vitro urethra model and methodology.
Results
When compared to the bacterial growth control, a significant difference in bacterial growth was found after insertion of the uncoated (P ≤ 0·001) and hydrophilic coated (P ≤ 0·009) catheters; no significant difference when a prototype catheter was inserted into the in vitro urethra model with either bacterial species tested (P ≥ 0·423).
Conclusion
The results presented support the hypothesis that a single catheter insertion can initiate a catheter‐associated urinary tract infection.
Significance and Impact of the Study
The in vitro urethra model and associated methodology were found to be reliable and reproducible (P ≥ 0·265) providing new research tool for the development and validation of emerging technologies in urological healthcare.</description><subject>Bacteria</subject><subject>Bacteria - growth & development</subject><subject>Bacteria - isolation & purification</subject><subject>Catheter-Related Infections - microbiology</subject><subject>Catheters</subject><subject>Displacement</subject><subject>E. coli (all potentially pathogenic types)</subject><subject>Equipment Contamination</subject><subject>Humans</subject><subject>In vitro methods and tests</subject><subject>infection</subject><subject>Insertion</subject><subject>Intermittent Urethral Catheterization - adverse effects</subject><subject>Intermittent Urethral Catheterization - instrumentation</subject><subject>Male</subject><subject>mechanism of action</subject><subject>Medical instruments</subject><subject>microbial contamination</subject><subject>Microorganisms</subject><subject>Models, Biological</subject><subject>New technology</subject><subject>staphylococci</subject><subject>Urethra</subject><subject>Urethra - microbiology</subject><subject>Urinary Catheters - microbiology</subject><subject>Urinary tract</subject><subject>Urinary tract infections</subject><subject>Urinary Tract Infections - microbiology</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE9LwzAYh4Mobk4PfgEpeNFDtyZp0tbbGP5loge9CSVr324ZbTKTdLJvb7ZOD4LvJW9-PDy8_BA6x9EQ-xktRTPEMaP0APUx5SwkPCGHuz0OWZSQHjqxdhlFmEaMH6MexUlKUs776ONVuIWegwpKaVe1KKAB5YKyNVLNA6kcmEY6t80KT4L_-9SCcVKrm0AESq-h9lGwls7ooDXgFkYEjS6hPkVHlagtnO3fAXq_u32bPITTl_vHyXgaFtQfHfIKU0Y4FQkvS2CCVYwUIsYsIzFNs4gzMitwNmM-SQmkacyTikBJBWQ8nsV0gK4678rozxasyxtpC6hroUC3NieURDRhFDOPXv5Bl7o1yl_nKZ4lDGd4K7zuqMJoaw1U-crIRphNjqN8W3nuK893lXv2Ym9sZw2Uv-RPxx4YdcCXrGHzvyl_Gj93ym9b9Ync</recordid><startdate>202004</startdate><enddate>202004</enddate><creator>Cortese, Y.J.</creator><creator>Wagner, V.E.</creator><creator>Tierney, M.</creator><creator>Scully, D.</creator><creator>Devine, D.M.</creator><creator>Fogarty, A.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1364-5583</orcidid><orcidid>https://orcid.org/0000-0001-9812-480X</orcidid></search><sort><creationdate>202004</creationdate><title>Pathogen displacement during intermittent catheter insertion: a novel in vitro urethra model</title><author>Cortese, Y.J. ; Wagner, V.E. ; Tierney, M. ; Scully, D. ; Devine, D.M. ; Fogarty, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3533-6f135263a76dde5a5f52ca4159243890652bc19b515982e88467f2ed3ae964b43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Bacteria</topic><topic>Bacteria - growth & development</topic><topic>Bacteria - isolation & purification</topic><topic>Catheter-Related Infections - microbiology</topic><topic>Catheters</topic><topic>Displacement</topic><topic>E. coli (all potentially pathogenic types)</topic><topic>Equipment Contamination</topic><topic>Humans</topic><topic>In vitro methods and tests</topic><topic>infection</topic><topic>Insertion</topic><topic>Intermittent Urethral Catheterization - adverse effects</topic><topic>Intermittent Urethral Catheterization - instrumentation</topic><topic>Male</topic><topic>mechanism of action</topic><topic>Medical instruments</topic><topic>microbial contamination</topic><topic>Microorganisms</topic><topic>Models, Biological</topic><topic>New technology</topic><topic>staphylococci</topic><topic>Urethra</topic><topic>Urethra - microbiology</topic><topic>Urinary Catheters - microbiology</topic><topic>Urinary tract</topic><topic>Urinary tract infections</topic><topic>Urinary Tract Infections - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cortese, Y.J.</creatorcontrib><creatorcontrib>Wagner, V.E.</creatorcontrib><creatorcontrib>Tierney, M.</creatorcontrib><creatorcontrib>Scully, D.</creatorcontrib><creatorcontrib>Devine, D.M.</creatorcontrib><creatorcontrib>Fogarty, A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cortese, Y.J.</au><au>Wagner, V.E.</au><au>Tierney, M.</au><au>Scully, D.</au><au>Devine, D.M.</au><au>Fogarty, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pathogen displacement during intermittent catheter insertion: a novel in vitro urethra model</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2020-04</date><risdate>2020</risdate><volume>128</volume><issue>4</issue><spage>1191</spage><epage>1200</epage><pages>1191-1200</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aim
To develop a novel in vitro urethra model and use it to determine if insertion of an intermittent urinary catheter (IC) displaces pathogenic bacteria from the urethral meatus along the urethra.
Methods
Displacement of microbial growth after catheter insertion was assessed using a novel in vitro urethra model. The in vitro urethra model utilized chromogenic agar and was inoculated with bacteria at one side of the artificial urethra channel, to act as a contaminated urethral meatus, before an IC was inserted into the channel. Three ICs types were used to validate the in vitro urethra model and methodology.
Results
When compared to the bacterial growth control, a significant difference in bacterial growth was found after insertion of the uncoated (P ≤ 0·001) and hydrophilic coated (P ≤ 0·009) catheters; no significant difference when a prototype catheter was inserted into the in vitro urethra model with either bacterial species tested (P ≥ 0·423).
Conclusion
The results presented support the hypothesis that a single catheter insertion can initiate a catheter‐associated urinary tract infection.
Significance and Impact of the Study
The in vitro urethra model and associated methodology were found to be reliable and reproducible (P ≥ 0·265) providing new research tool for the development and validation of emerging technologies in urological healthcare.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>31782866</pmid><doi>10.1111/jam.14533</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-1364-5583</orcidid><orcidid>https://orcid.org/0000-0001-9812-480X</orcidid></addata></record> |
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| source | MEDLINE; Wiley Journals; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Bacteria Bacteria - growth & development Bacteria - isolation & purification Catheter-Related Infections - microbiology Catheters Displacement E. coli (all potentially pathogenic types) Equipment Contamination Humans In vitro methods and tests infection Insertion Intermittent Urethral Catheterization - adverse effects Intermittent Urethral Catheterization - instrumentation Male mechanism of action Medical instruments microbial contamination Microorganisms Models, Biological New technology staphylococci Urethra Urethra - microbiology Urinary Catheters - microbiology Urinary tract Urinary tract infections Urinary Tract Infections - microbiology |
| title | Pathogen displacement during intermittent catheter insertion: a novel in vitro urethra model |
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