Simultaneous functional imaging of neuronal and photoreceptor layers in living human retina
Functional retinal imaging, especially of neuronal activity non-invasively in humans, is of tremendous interest. Although the activation of photoreceptor cells (PRCs) could be detected in humans, imaging the function of other retinal neurons had been so far hardly possible. Here, using phase-sensiti...
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| Veröffentlicht in: | Optics letters 2019-12, Vol.44 (23), p.5671-5674 |
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| container_title | Optics letters |
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| creator | Pfäffle, Clara Spahr, Hendrik Kutzner, Lisa Burhan, Sazan Hilge, Felix Miura, Yoko Hüttmann, Gereon Hillmann, Dierck |
| description | Functional retinal imaging, especially of neuronal activity non-invasively in humans, is of tremendous interest. Although the activation of photoreceptor cells (PRCs) could be detected in humans, imaging the function of other retinal neurons had been so far hardly possible. Here, using phase-sensitive full-field swept-source optical coherence tomography (FF-SS-OCT), we show simultaneous imaging of the activation in the photoreceptor and ganglion cell layer/inner plexiform layer (GCL/IPL). The signals from the GCL/IPL are 10-fold smaller than those from the PRC and were detectable only using algorithms for suppression of motion artifacts and pulsatile blood flow in the retinal vessels. FF-SS-OCT with improved phase evaluation algorithms, therefore, allowed us to map functional connections between PRC and GCL/IPL, confirming previous ex vivo results. The demonstrated functional imaging of retinal neuronal layers can be a valuable tool in diagnostics and basic research. |
| doi_str_mv | 10.1364/OL.44.005671 |
| format | Article |
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Although the activation of photoreceptor cells (PRCs) could be detected in humans, imaging the function of other retinal neurons had been so far hardly possible. Here, using phase-sensitive full-field swept-source optical coherence tomography (FF-SS-OCT), we show simultaneous imaging of the activation in the photoreceptor and ganglion cell layer/inner plexiform layer (GCL/IPL). The signals from the GCL/IPL are 10-fold smaller than those from the PRC and were detectable only using algorithms for suppression of motion artifacts and pulsatile blood flow in the retinal vessels. FF-SS-OCT with improved phase evaluation algorithms, therefore, allowed us to map functional connections between PRC and GCL/IPL, confirming previous ex vivo results. The demonstrated functional imaging of retinal neuronal layers can be a valuable tool in diagnostics and basic research.</description><identifier>ISSN: 0146-9592</identifier><identifier>EISSN: 1539-4794</identifier><identifier>DOI: 10.1364/OL.44.005671</identifier><identifier>PMID: 31774751</identifier><language>eng</language><publisher>United States: Optical Society of America</publisher><subject>Activation ; Algorithms ; Blood flow ; Blood vessels ; Cell Survival ; Humans ; Neurons - cytology ; Optical Coherence Tomography ; Photoreceptor Cells, Vertebrate - cytology ; Photoreceptors ; Retina ; Retinal images ; Time Factors ; Tomography, Optical Coherence</subject><ispartof>Optics letters, 2019-12, Vol.44 (23), p.5671-5674</ispartof><rights>Copyright Optical Society of America Dec 1, 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-1894ef6afa2f0db8d9f6c3f51ccd137c63d2dd04bddce74f530116340ef1efe13</citedby><cites>FETCH-LOGICAL-c319t-1894ef6afa2f0db8d9f6c3f51ccd137c63d2dd04bddce74f530116340ef1efe13</cites><orcidid>0000-0002-5051-3037 ; 0000-0001-7659-7550</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3245,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31774751$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pfäffle, Clara</creatorcontrib><creatorcontrib>Spahr, Hendrik</creatorcontrib><creatorcontrib>Kutzner, Lisa</creatorcontrib><creatorcontrib>Burhan, Sazan</creatorcontrib><creatorcontrib>Hilge, Felix</creatorcontrib><creatorcontrib>Miura, Yoko</creatorcontrib><creatorcontrib>Hüttmann, Gereon</creatorcontrib><creatorcontrib>Hillmann, Dierck</creatorcontrib><title>Simultaneous functional imaging of neuronal and photoreceptor layers in living human retina</title><title>Optics letters</title><addtitle>Opt Lett</addtitle><description>Functional retinal imaging, especially of neuronal activity non-invasively in humans, is of tremendous interest. Although the activation of photoreceptor cells (PRCs) could be detected in humans, imaging the function of other retinal neurons had been so far hardly possible. Here, using phase-sensitive full-field swept-source optical coherence tomography (FF-SS-OCT), we show simultaneous imaging of the activation in the photoreceptor and ganglion cell layer/inner plexiform layer (GCL/IPL). The signals from the GCL/IPL are 10-fold smaller than those from the PRC and were detectable only using algorithms for suppression of motion artifacts and pulsatile blood flow in the retinal vessels. FF-SS-OCT with improved phase evaluation algorithms, therefore, allowed us to map functional connections between PRC and GCL/IPL, confirming previous ex vivo results. The demonstrated functional imaging of retinal neuronal layers can be a valuable tool in diagnostics and basic research.</description><subject>Activation</subject><subject>Algorithms</subject><subject>Blood flow</subject><subject>Blood vessels</subject><subject>Cell Survival</subject><subject>Humans</subject><subject>Neurons - cytology</subject><subject>Optical Coherence Tomography</subject><subject>Photoreceptor Cells, Vertebrate - cytology</subject><subject>Photoreceptors</subject><subject>Retina</subject><subject>Retinal images</subject><subject>Time Factors</subject><subject>Tomography, Optical Coherence</subject><issn>0146-9592</issn><issn>1539-4794</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkD1PwzAQhi0EoqWwMSNLLAyk-GI7bkZU8SVF6gBMDJHrjzZV4hQ7Ruq_JyWFgemVTs-9p3sQugQyBZqxu0UxZWxKCM8EHKExcJonTOTsGI0JsCzJeZ6O0FkIG0JIJig9RSMKQjDBYYw-Xqsm1p10po0B2-hUV7VO1rhq5KpyK9xa7Ez0PzPpNN6u2671RpltH7iWO-MDrhyuq689vo6NdNibrnLyHJ1YWQdzccgJen98eJs_J8Xi6WV-XySKQt4lMMuZsZm0MrVEL2c6t5miloNSGqhQGdWp1oQttVZGMMspAcgoI8aCsQboBN0MvVvffkYTurKpgjJ1PXxVpv0Z3u_wvEev_6GbNvr-tz2Vpgx4KnhP3Q6U8m0I3thy63sfflcCKffSy0VRMlYO0nv86lAal43Rf_CvZfoN8dR90A</recordid><startdate>20191201</startdate><enddate>20191201</enddate><creator>Pfäffle, Clara</creator><creator>Spahr, Hendrik</creator><creator>Kutzner, Lisa</creator><creator>Burhan, Sazan</creator><creator>Hilge, Felix</creator><creator>Miura, Yoko</creator><creator>Hüttmann, Gereon</creator><creator>Hillmann, Dierck</creator><general>Optical Society of America</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7U5</scope><scope>8FD</scope><scope>H8D</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5051-3037</orcidid><orcidid>https://orcid.org/0000-0001-7659-7550</orcidid></search><sort><creationdate>20191201</creationdate><title>Simultaneous functional imaging of neuronal and photoreceptor layers in living human retina</title><author>Pfäffle, Clara ; Spahr, Hendrik ; Kutzner, Lisa ; Burhan, Sazan ; Hilge, Felix ; Miura, Yoko ; Hüttmann, Gereon ; Hillmann, Dierck</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-1894ef6afa2f0db8d9f6c3f51ccd137c63d2dd04bddce74f530116340ef1efe13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Activation</topic><topic>Algorithms</topic><topic>Blood flow</topic><topic>Blood vessels</topic><topic>Cell Survival</topic><topic>Humans</topic><topic>Neurons - cytology</topic><topic>Optical Coherence Tomography</topic><topic>Photoreceptor Cells, Vertebrate - cytology</topic><topic>Photoreceptors</topic><topic>Retina</topic><topic>Retinal images</topic><topic>Time Factors</topic><topic>Tomography, Optical Coherence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pfäffle, Clara</creatorcontrib><creatorcontrib>Spahr, Hendrik</creatorcontrib><creatorcontrib>Kutzner, Lisa</creatorcontrib><creatorcontrib>Burhan, Sazan</creatorcontrib><creatorcontrib>Hilge, Felix</creatorcontrib><creatorcontrib>Miura, Yoko</creatorcontrib><creatorcontrib>Hüttmann, Gereon</creatorcontrib><creatorcontrib>Hillmann, Dierck</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Aerospace Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Optics letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pfäffle, Clara</au><au>Spahr, Hendrik</au><au>Kutzner, Lisa</au><au>Burhan, Sazan</au><au>Hilge, Felix</au><au>Miura, Yoko</au><au>Hüttmann, Gereon</au><au>Hillmann, Dierck</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simultaneous functional imaging of neuronal and photoreceptor layers in living human retina</atitle><jtitle>Optics letters</jtitle><addtitle>Opt Lett</addtitle><date>2019-12-01</date><risdate>2019</risdate><volume>44</volume><issue>23</issue><spage>5671</spage><epage>5674</epage><pages>5671-5674</pages><issn>0146-9592</issn><eissn>1539-4794</eissn><abstract>Functional retinal imaging, especially of neuronal activity non-invasively in humans, is of tremendous interest. Although the activation of photoreceptor cells (PRCs) could be detected in humans, imaging the function of other retinal neurons had been so far hardly possible. Here, using phase-sensitive full-field swept-source optical coherence tomography (FF-SS-OCT), we show simultaneous imaging of the activation in the photoreceptor and ganglion cell layer/inner plexiform layer (GCL/IPL). The signals from the GCL/IPL are 10-fold smaller than those from the PRC and were detectable only using algorithms for suppression of motion artifacts and pulsatile blood flow in the retinal vessels. FF-SS-OCT with improved phase evaluation algorithms, therefore, allowed us to map functional connections between PRC and GCL/IPL, confirming previous ex vivo results. 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| ispartof | Optics letters, 2019-12, Vol.44 (23), p.5671-5674 |
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| language | eng |
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| source | MEDLINE; Optica Publishing Group Journals |
| subjects | Activation Algorithms Blood flow Blood vessels Cell Survival Humans Neurons - cytology Optical Coherence Tomography Photoreceptor Cells, Vertebrate - cytology Photoreceptors Retina Retinal images Time Factors Tomography, Optical Coherence |
| title | Simultaneous functional imaging of neuronal and photoreceptor layers in living human retina |
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