Study on the Expression of KDM6B of Osteoblast in Diabetic Environment

Study on the Expression of KDM6B of Osteoblast in Diabetic Environment

OBJECTIVETo investigate the ability of osteogenic differentiation and the expression of histone demethylases KDM6B in bone marrow mesenchymal stem cells (BMSCs) in diabetic environment. METHODSDiabetic model rats was successfully established, and BMSCs from diabetic model rats and normal rats were i...

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Veröffentlicht in:Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban 2019-09, Vol.50 (5), p.660-665
Hauptverfasser: Tang, Yu, Hu, Yun, Luo, Dan, Ding, Xiao-Qian, Li, Cai-Yu, Zheng, Lei-Lei
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container_end_page 665
container_issue 5
container_start_page 660
container_title Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban
container_volume 50
creator Tang, Yu
Hu, Yun
Luo, Dan
Ding, Xiao-Qian
Li, Cai-Yu
Zheng, Lei-Lei
description OBJECTIVETo investigate the ability of osteogenic differentiation and the expression of histone demethylases KDM6B in bone marrow mesenchymal stem cells (BMSCs) in diabetic environment. METHODSDiabetic model rats was successfully established, and BMSCs from diabetic model rats and normal rats were isolated and cultured for further study. When cultured cells, we added high concentration of glucose and advanced glycosylation products (AGE) in the medium to imitating the diabetic environment. BMSCs were divided into 6 groups: diabetes group (derived from diabets SD rats), normal group (derived from normal SD rats), high glucose group (30 mmol/L D-glucose), normal glucose group (5.5 mmol/L D-glucose), AGE group (AGE 300 μg/mL) and BSA group (BSA 300 μg/mL). BMSCs in diabetes group were derived from diabetes SD rats, while others were derived from normal SD rats. After 7 d of osteogenic induction, the cells were examined the ability of osteogenic differentiation by alkaline phosphatase (ALP) staining, the transcri
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METHODSDiabetic model rats was successfully established, and BMSCs from diabetic model rats and normal rats were isolated and cultured for further study. When cultured cells, we added high concentration of glucose and advanced glycosylation products (AGE) in the medium to imitating the diabetic environment. BMSCs were divided into 6 groups: diabetes group (derived from diabets SD rats), normal group (derived from normal SD rats), high glucose group (30 mmol/L D-glucose), normal glucose group (5.5 mmol/L D-glucose), AGE group (AGE 300 μg/mL) and BSA group (BSA 300 μg/mL). BMSCs in diabetes group were derived from diabetes SD rats, while others were derived from normal SD rats. After 7 d of osteogenic induction, the cells were examined the ability of osteogenic differentiation by alkaline phosphatase (ALP) staining, the transcri</description><identifier>ISSN: 1672-173X</identifier><language>chi</language><ispartof>Sichuan da xue xue bao. Journal of Sichuan University. 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