Photobiomodulation reduces cell death and cytokine production in C2C12 cells exposed to Bothrops venoms
Snakebites caused by the genus Bothrops are often associated with severe and complex local manifestations such as edema, pain, hemorrhage, and myonecrosis. Conventional treatment minimizes the systemic effects of venom; however, their local action is not neutralized. The purpose of this study was to...
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| Veröffentlicht in: | Lasers in medical science 2020-07, Vol.35 (5), p.1047-1054 |
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| creator | Gouveia, Viviane Almeida Pisete, Flavia Regina Ferreira Soares Wagner, Cristiane Luize Rocha Dalboni, Maria Aparecida de Oliveira, Ana Paula Ligeiro Cogo, José Carlos Zamuner, Stella Regina |
| description | Snakebites caused by the genus
Bothrops
are often associated with severe and complex local manifestations such as edema, pain, hemorrhage, and myonecrosis. Conventional treatment minimizes the systemic effects of venom; however, their local action is not neutralized. The purpose of this study was to evaluate the effect of photobiomodulation (PBM) on C2C12 muscle cells exposed to
B. jararaca
,
B. jararacussu
, and
B. moojeni
venoms on events involved in cell death and the release of inflammatory mediators. Cells were exposed to venoms and immediately irradiated with low-level laser (LLL) application in continuous wave at the wavelength of 660 nm, energy density of 4.4 J/cm
2
, power of 10 mW, area of 0.045 cm
2
, and time of 20 s. Cell integrity was analyzed by phase contrast microscope and cell death was performed by flow cytometry. In addition, interleukin IL1-β, IL-6, and IL-10 levels were measured in the supernatant. Our results showed that the application of PBM increases cell viability and decreases cell death by apoptosis and necrosis. Moreover, the release of pro-inflammatory interleukins was also reduced. The data reported here indicate that PBM resulted in cytoprotection on myoblast C2C12 cells after venom exposure. This protection involves the modulation of cell death mechanism and decreased pro-inflammatory cytokine release. |
| doi_str_mv | 10.1007/s10103-019-02884-4 |
| format | Article |
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Bothrops
are often associated with severe and complex local manifestations such as edema, pain, hemorrhage, and myonecrosis. Conventional treatment minimizes the systemic effects of venom; however, their local action is not neutralized. The purpose of this study was to evaluate the effect of photobiomodulation (PBM) on C2C12 muscle cells exposed to
B. jararaca
,
B. jararacussu
, and
B. moojeni
venoms on events involved in cell death and the release of inflammatory mediators. Cells were exposed to venoms and immediately irradiated with low-level laser (LLL) application in continuous wave at the wavelength of 660 nm, energy density of 4.4 J/cm
2
, power of 10 mW, area of 0.045 cm
2
, and time of 20 s. Cell integrity was analyzed by phase contrast microscope and cell death was performed by flow cytometry. In addition, interleukin IL1-β, IL-6, and IL-10 levels were measured in the supernatant. Our results showed that the application of PBM increases cell viability and decreases cell death by apoptosis and necrosis. Moreover, the release of pro-inflammatory interleukins was also reduced. The data reported here indicate that PBM resulted in cytoprotection on myoblast C2C12 cells after venom exposure. This protection involves the modulation of cell death mechanism and decreased pro-inflammatory cytokine release.</description><identifier>ISSN: 0268-8921</identifier><identifier>EISSN: 1435-604X</identifier><identifier>DOI: 10.1007/s10103-019-02884-4</identifier><language>eng</language><publisher>London: Springer London</publisher><subject>Apoptosis ; Bothrops ; Cell death ; Cell viability ; Continuous radiation ; Cytokines ; Dentistry ; Edema ; Exposure ; Flow cytometry ; Flux density ; Hemorrhage ; Inflammation ; Interleukin 1 ; Interleukin 10 ; Interleukin 6 ; Lasers ; Light therapy ; Medicine ; Medicine & Public Health ; Mortality ; Muscles ; Myonecrosis ; Necrosis ; Optical Devices ; Optics ; Original Article ; Pain ; Phase contrast ; Photonics ; Quantum Optics ; Venom</subject><ispartof>Lasers in medical science, 2020-07, Vol.35 (5), p.1047-1054</ispartof><rights>Springer-Verlag London Ltd., part of Springer Nature 2019</rights><rights>Springer-Verlag London Ltd., part of Springer Nature 2019.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-86a7114183b020a3f405e788aa83bb7fa56321b37867bcd3ed83e54872a5643c3</citedby><cites>FETCH-LOGICAL-c352t-86a7114183b020a3f405e788aa83bb7fa56321b37867bcd3ed83e54872a5643c3</cites><orcidid>0000-0002-4694-7617</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10103-019-02884-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10103-019-02884-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids></links><search><creatorcontrib>Gouveia, Viviane Almeida</creatorcontrib><creatorcontrib>Pisete, Flavia Regina Ferreira Soares</creatorcontrib><creatorcontrib>Wagner, Cristiane Luize Rocha</creatorcontrib><creatorcontrib>Dalboni, Maria Aparecida</creatorcontrib><creatorcontrib>de Oliveira, Ana Paula Ligeiro</creatorcontrib><creatorcontrib>Cogo, José Carlos</creatorcontrib><creatorcontrib>Zamuner, Stella Regina</creatorcontrib><title>Photobiomodulation reduces cell death and cytokine production in C2C12 cells exposed to Bothrops venoms</title><title>Lasers in medical science</title><addtitle>Lasers Med Sci</addtitle><description>Snakebites caused by the genus
Bothrops
are often associated with severe and complex local manifestations such as edema, pain, hemorrhage, and myonecrosis. Conventional treatment minimizes the systemic effects of venom; however, their local action is not neutralized. The purpose of this study was to evaluate the effect of photobiomodulation (PBM) on C2C12 muscle cells exposed to
B. jararaca
,
B. jararacussu
, and
B. moojeni
venoms on events involved in cell death and the release of inflammatory mediators. Cells were exposed to venoms and immediately irradiated with low-level laser (LLL) application in continuous wave at the wavelength of 660 nm, energy density of 4.4 J/cm
2
, power of 10 mW, area of 0.045 cm
2
, and time of 20 s. Cell integrity was analyzed by phase contrast microscope and cell death was performed by flow cytometry. In addition, interleukin IL1-β, IL-6, and IL-10 levels were measured in the supernatant. Our results showed that the application of PBM increases cell viability and decreases cell death by apoptosis and necrosis. Moreover, the release of pro-inflammatory interleukins was also reduced. The data reported here indicate that PBM resulted in cytoprotection on myoblast C2C12 cells after venom exposure. This protection involves the modulation of cell death mechanism and decreased pro-inflammatory cytokine release.</description><subject>Apoptosis</subject><subject>Bothrops</subject><subject>Cell death</subject><subject>Cell viability</subject><subject>Continuous radiation</subject><subject>Cytokines</subject><subject>Dentistry</subject><subject>Edema</subject><subject>Exposure</subject><subject>Flow cytometry</subject><subject>Flux density</subject><subject>Hemorrhage</subject><subject>Inflammation</subject><subject>Interleukin 1</subject><subject>Interleukin 10</subject><subject>Interleukin 6</subject><subject>Lasers</subject><subject>Light therapy</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mortality</subject><subject>Muscles</subject><subject>Myonecrosis</subject><subject>Necrosis</subject><subject>Optical Devices</subject><subject>Optics</subject><subject>Original Article</subject><subject>Pain</subject><subject>Phase contrast</subject><subject>Photonics</subject><subject>Quantum Optics</subject><subject>Venom</subject><issn>0268-8921</issn><issn>1435-604X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kE1LAzEQhoMoWKt_wFPAi5fVmUx2kx61-AWCHhS8hexu2m5tNzXZFfvvTVtB8OBpYOaZl5eHsVOECwRQlxEBgTLAUQZCa5nJPTZASXlWgHzbZwMQhc70SOAhO4pxDoCqQBqw6fPMd75s_NLX_cJ2jW95cHVfucgrt1jw2tluxm1b82rd-femdXwVEltt0ablYzFGsWUjd18rH13NO8-vfTcLfhX5p2v9Mh6zg4ldRHfyM4fs9fbmZXyfPT7dPYyvHrOKctFlurAKUaKmEgRYmkjIndLa2rQp1cTmBQksSelClVVNrtbkcqmVSBdJFQ3Z-S43lfzoXezMsombcrZ1vo9GEKp8RJDgITv7g859H9rUzgiJoshJACVK7Kgq-BiDm5hVaJY2rA2C2bg3O_cmuTdb92YTTbunmOB26sJv9D9f3ytkhlw</recordid><startdate>20200701</startdate><enddate>20200701</enddate><creator>Gouveia, Viviane 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reduces cell death and cytokine production in C2C12 cells exposed to Bothrops venoms</title><author>Gouveia, Viviane Almeida ; Pisete, Flavia Regina Ferreira Soares ; Wagner, Cristiane Luize Rocha ; Dalboni, Maria Aparecida ; de Oliveira, Ana Paula Ligeiro ; Cogo, José Carlos ; Zamuner, Stella Regina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-86a7114183b020a3f405e788aa83bb7fa56321b37867bcd3ed83e54872a5643c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Apoptosis</topic><topic>Bothrops</topic><topic>Cell death</topic><topic>Cell viability</topic><topic>Continuous radiation</topic><topic>Cytokines</topic><topic>Dentistry</topic><topic>Edema</topic><topic>Exposure</topic><topic>Flow cytometry</topic><topic>Flux density</topic><topic>Hemorrhage</topic><topic>Inflammation</topic><topic>Interleukin 1</topic><topic>Interleukin 10</topic><topic>Interleukin 6</topic><topic>Lasers</topic><topic>Light therapy</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mortality</topic><topic>Muscles</topic><topic>Myonecrosis</topic><topic>Necrosis</topic><topic>Optical Devices</topic><topic>Optics</topic><topic>Original Article</topic><topic>Pain</topic><topic>Phase contrast</topic><topic>Photonics</topic><topic>Quantum Optics</topic><topic>Venom</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gouveia, Viviane Almeida</creatorcontrib><creatorcontrib>Pisete, Flavia Regina Ferreira Soares</creatorcontrib><creatorcontrib>Wagner, Cristiane Luize Rocha</creatorcontrib><creatorcontrib>Dalboni, Maria Aparecida</creatorcontrib><creatorcontrib>de Oliveira, Ana Paula Ligeiro</creatorcontrib><creatorcontrib>Cogo, José Carlos</creatorcontrib><creatorcontrib>Zamuner, Stella Regina</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central 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Luize Rocha</au><au>Dalboni, Maria Aparecida</au><au>de Oliveira, Ana Paula Ligeiro</au><au>Cogo, José Carlos</au><au>Zamuner, Stella Regina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Photobiomodulation reduces cell death and cytokine production in C2C12 cells exposed to Bothrops venoms</atitle><jtitle>Lasers in medical science</jtitle><stitle>Lasers Med Sci</stitle><date>2020-07-01</date><risdate>2020</risdate><volume>35</volume><issue>5</issue><spage>1047</spage><epage>1054</epage><pages>1047-1054</pages><issn>0268-8921</issn><eissn>1435-604X</eissn><abstract>Snakebites caused by the genus
Bothrops
are often associated with severe and complex local manifestations such as edema, pain, hemorrhage, and myonecrosis. Conventional treatment minimizes the systemic effects of venom; however, their local action is not neutralized. The purpose of this study was to evaluate the effect of photobiomodulation (PBM) on C2C12 muscle cells exposed to
B. jararaca
,
B. jararacussu
, and
B. moojeni
venoms on events involved in cell death and the release of inflammatory mediators. Cells were exposed to venoms and immediately irradiated with low-level laser (LLL) application in continuous wave at the wavelength of 660 nm, energy density of 4.4 J/cm
2
, power of 10 mW, area of 0.045 cm
2
, and time of 20 s. Cell integrity was analyzed by phase contrast microscope and cell death was performed by flow cytometry. In addition, interleukin IL1-β, IL-6, and IL-10 levels were measured in the supernatant. Our results showed that the application of PBM increases cell viability and decreases cell death by apoptosis and necrosis. Moreover, the release of pro-inflammatory interleukins was also reduced. The data reported here indicate that PBM resulted in cytoprotection on myoblast C2C12 cells after venom exposure. This protection involves the modulation of cell death mechanism and decreased pro-inflammatory cytokine release.</abstract><cop>London</cop><pub>Springer London</pub><doi>10.1007/s10103-019-02884-4</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-4694-7617</orcidid></addata></record> |
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| ispartof | Lasers in medical science, 2020-07, Vol.35 (5), p.1047-1054 |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Apoptosis Bothrops Cell death Cell viability Continuous radiation Cytokines Dentistry Edema Exposure Flow cytometry Flux density Hemorrhage Inflammation Interleukin 1 Interleukin 10 Interleukin 6 Lasers Light therapy Medicine Medicine & Public Health Mortality Muscles Myonecrosis Necrosis Optical Devices Optics Original Article Pain Phase contrast Photonics Quantum Optics Venom |
| title | Photobiomodulation reduces cell death and cytokine production in C2C12 cells exposed to Bothrops venoms |
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