The Addition of Polar Organic Solvent Vapors During the Analysis of Proteins by DESI-MS
Exposure of electrospray droplets to organic vapors was shown to dramatically reduce alkali-metal adduction on protein ions and shift protein charge states. Since DESI-MS is affected by similar adduct species as ESI-MS and shares similar ionization mechanisms, polar organic vapor additives should li...
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| Veröffentlicht in: | Journal of the American Society for Mass Spectrometry 2019-12, Vol.30 (12), p.2571-2575 |
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| container_title | Journal of the American Society for Mass Spectrometry |
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| creator | Javanshad, Roshan Maser, Tara L. Honarvar, Elahe Venter, Andre R. |
| description | Exposure of electrospray droplets to organic vapors was shown to dramatically reduce alkali-metal adduction on protein ions and shift protein charge states. Since DESI-MS is affected by similar adduct species as ESI-MS and shares similar ionization mechanisms, polar organic vapor additives should likewise also improve the DESI-MS analysis of proteins. Here the DESI spray was exposed to a variety of polar organic vapor additives. Head space vapors of polar organic solvents were entrained in nitrogen gas and delivered to the atmosphere inside a semi-enclosed plastic enclosure surrounding the spray plume. The vapors of acetone, acetonitrile, ethyl acetate, methanol, and water were investigated. Vapor dependent effects were observed with respect to changes in protein charge state distributions and signal intensities. With ethyl acetate vapor addition, the signal intensities of all proteins investigated were significantly increased, including proteins larger than 25 kDa such as carbonic anhydrase II and bovine serum albumin. |
| doi_str_mv | 10.1007/s13361-019-02345-w |
| format | Article |
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Since DESI-MS is affected by similar adduct species as ESI-MS and shares similar ionization mechanisms, polar organic vapor additives should likewise also improve the DESI-MS analysis of proteins. Here the DESI spray was exposed to a variety of polar organic vapor additives. Head space vapors of polar organic solvents were entrained in nitrogen gas and delivered to the atmosphere inside a semi-enclosed plastic enclosure surrounding the spray plume. The vapors of acetone, acetonitrile, ethyl acetate, methanol, and water were investigated. Vapor dependent effects were observed with respect to changes in protein charge state distributions and signal intensities. With ethyl acetate vapor addition, the signal intensities of all proteins investigated were significantly increased, including proteins larger than 25 kDa such as carbonic anhydrase II and bovine serum albumin.</description><identifier>ISSN: 1044-0305</identifier><identifier>EISSN: 1879-1123</identifier><identifier>DOI: 10.1007/s13361-019-02345-w</identifier><identifier>PMID: 31758521</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Acetates - chemistry ; Acetone ; Acetone - chemistry ; Acetonitrile ; Acetonitriles - chemistry ; Additives ; Alkali metals ; Analytical Chemistry ; Animals ; Bioinformatics ; Biotechnology ; Carbonic anhydrase ; Carbonic Anhydrase II - analysis ; Cattle ; Charge distribution ; Chemistry ; Chemistry and Materials Science ; Cytochromes c - analysis ; Equipment Design ; Ethyl acetate ; Horses ; Ions ; Mass spectrometry ; Methanol - chemistry ; Organic Chemistry ; Proteins ; Proteins - analysis ; Proteomics ; Serum albumin ; Serum Albumin, Bovine - analysis ; Short Communication ; Solvents ; Solvents - chemistry ; Spectrometry, Mass, Electrospray Ionization - instrumentation ; Spectrometry, Mass, Electrospray Ionization - methods ; Vapors ; Volatilization ; Water - chemistry</subject><ispartof>Journal of the American Society for Mass Spectrometry, 2019-12, Vol.30 (12), p.2571-2575</ispartof><rights>American Society for Mass Spectrometry 2019</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-f8d5d54fde6bbbc72a158daf14bd34d0b966575eb18ea09b9549ecf99aee98a73</citedby><cites>FETCH-LOGICAL-c375t-f8d5d54fde6bbbc72a158daf14bd34d0b966575eb18ea09b9549ecf99aee98a73</cites><orcidid>0000-0001-6912-7446</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13361-019-02345-w$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13361-019-02345-w$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27907,27908,41471,42540,51302</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31758521$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Javanshad, Roshan</creatorcontrib><creatorcontrib>Maser, Tara L.</creatorcontrib><creatorcontrib>Honarvar, Elahe</creatorcontrib><creatorcontrib>Venter, Andre R.</creatorcontrib><title>The Addition of Polar Organic Solvent Vapors During the Analysis of Proteins by DESI-MS</title><title>Journal of the American Society for Mass Spectrometry</title><addtitle>J. Am. Soc. Mass Spectrom</addtitle><addtitle>J Am Soc Mass Spectrom</addtitle><description>Exposure of electrospray droplets to organic vapors was shown to dramatically reduce alkali-metal adduction on protein ions and shift protein charge states. Since DESI-MS is affected by similar adduct species as ESI-MS and shares similar ionization mechanisms, polar organic vapor additives should likewise also improve the DESI-MS analysis of proteins. Here the DESI spray was exposed to a variety of polar organic vapor additives. Head space vapors of polar organic solvents were entrained in nitrogen gas and delivered to the atmosphere inside a semi-enclosed plastic enclosure surrounding the spray plume. The vapors of acetone, acetonitrile, ethyl acetate, methanol, and water were investigated. Vapor dependent effects were observed with respect to changes in protein charge state distributions and signal intensities. With ethyl acetate vapor addition, the signal intensities of all proteins investigated were significantly increased, including proteins larger than 25 kDa such as carbonic anhydrase II and bovine serum albumin.</description><subject>Acetates - chemistry</subject><subject>Acetone</subject><subject>Acetone - chemistry</subject><subject>Acetonitrile</subject><subject>Acetonitriles - chemistry</subject><subject>Additives</subject><subject>Alkali metals</subject><subject>Analytical Chemistry</subject><subject>Animals</subject><subject>Bioinformatics</subject><subject>Biotechnology</subject><subject>Carbonic anhydrase</subject><subject>Carbonic Anhydrase II - analysis</subject><subject>Cattle</subject><subject>Charge distribution</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Cytochromes c - analysis</subject><subject>Equipment Design</subject><subject>Ethyl acetate</subject><subject>Horses</subject><subject>Ions</subject><subject>Mass spectrometry</subject><subject>Methanol - chemistry</subject><subject>Organic Chemistry</subject><subject>Proteins</subject><subject>Proteins - analysis</subject><subject>Proteomics</subject><subject>Serum albumin</subject><subject>Serum Albumin, Bovine - analysis</subject><subject>Short Communication</subject><subject>Solvents</subject><subject>Solvents - chemistry</subject><subject>Spectrometry, Mass, Electrospray Ionization - instrumentation</subject><subject>Spectrometry, Mass, Electrospray Ionization - methods</subject><subject>Vapors</subject><subject>Volatilization</subject><subject>Water - chemistry</subject><issn>1044-0305</issn><issn>1879-1123</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9PwjAchhujEUS_gAfTxIuXaruudD0S_EeCwQTUY9OuHY6MFdtNwrd3A9TEg6f-Ds_7Nu8DwDnB1wRjfhMIpX2CMBEIRzRmaH0AuiThAhES0cPmxnGMMMWsA05CWGBMOBb8GHQo4SxhEemCt9m7hQNj8ip3JXQZfHaF8nDi56rMUzh1xactK_iqVs4HeFv7vJzDqs2UqtiEPGwz3lU2LwPUG3h7Nx2hp-kpOMpUEezZ_u2Bl_u72fARjScPo-FgjFLKWYWyxDDD4szYvtY65ZEiLDEqI7E2NDZYi36fcWY1SazCQgsWC5tmQihrRaI47YGrXe_Ku4_ahkou85DaolCldXWQUTtVNMNb9PIPunC1b2a0VMQZpUnSUtGOSr0LwdtMrny-VH4jCZatdrnTLhvtcqtdrpvQxb661ktrfiLfnhuA7oCwag1a__v3P7Vf3DWM_Q</recordid><startdate>20191201</startdate><enddate>20191201</enddate><creator>Javanshad, Roshan</creator><creator>Maser, Tara L.</creator><creator>Honarvar, Elahe</creator><creator>Venter, Andre R.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6912-7446</orcidid></search><sort><creationdate>20191201</creationdate><title>The Addition of Polar Organic Solvent Vapors During the Analysis of Proteins by DESI-MS</title><author>Javanshad, Roshan ; Maser, Tara L. ; Honarvar, Elahe ; Venter, Andre R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-f8d5d54fde6bbbc72a158daf14bd34d0b966575eb18ea09b9549ecf99aee98a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Acetates - chemistry</topic><topic>Acetone</topic><topic>Acetone - chemistry</topic><topic>Acetonitrile</topic><topic>Acetonitriles - chemistry</topic><topic>Additives</topic><topic>Alkali metals</topic><topic>Analytical Chemistry</topic><topic>Animals</topic><topic>Bioinformatics</topic><topic>Biotechnology</topic><topic>Carbonic anhydrase</topic><topic>Carbonic Anhydrase II - analysis</topic><topic>Cattle</topic><topic>Charge distribution</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Cytochromes c - analysis</topic><topic>Equipment Design</topic><topic>Ethyl acetate</topic><topic>Horses</topic><topic>Ions</topic><topic>Mass spectrometry</topic><topic>Methanol - chemistry</topic><topic>Organic Chemistry</topic><topic>Proteins</topic><topic>Proteins - analysis</topic><topic>Proteomics</topic><topic>Serum albumin</topic><topic>Serum Albumin, Bovine - analysis</topic><topic>Short Communication</topic><topic>Solvents</topic><topic>Solvents - chemistry</topic><topic>Spectrometry, Mass, Electrospray Ionization - instrumentation</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><topic>Vapors</topic><topic>Volatilization</topic><topic>Water - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Javanshad, Roshan</creatorcontrib><creatorcontrib>Maser, Tara L.</creatorcontrib><creatorcontrib>Honarvar, Elahe</creatorcontrib><creatorcontrib>Venter, Andre R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the American Society for Mass Spectrometry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Javanshad, Roshan</au><au>Maser, Tara L.</au><au>Honarvar, Elahe</au><au>Venter, Andre R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Addition of Polar Organic Solvent Vapors During the Analysis of Proteins by DESI-MS</atitle><jtitle>Journal of the American Society for Mass Spectrometry</jtitle><stitle>J. Am. Soc. Mass Spectrom</stitle><addtitle>J Am Soc Mass Spectrom</addtitle><date>2019-12-01</date><risdate>2019</risdate><volume>30</volume><issue>12</issue><spage>2571</spage><epage>2575</epage><pages>2571-2575</pages><issn>1044-0305</issn><eissn>1879-1123</eissn><abstract>Exposure of electrospray droplets to organic vapors was shown to dramatically reduce alkali-metal adduction on protein ions and shift protein charge states. Since DESI-MS is affected by similar adduct species as ESI-MS and shares similar ionization mechanisms, polar organic vapor additives should likewise also improve the DESI-MS analysis of proteins. Here the DESI spray was exposed to a variety of polar organic vapor additives. Head space vapors of polar organic solvents were entrained in nitrogen gas and delivered to the atmosphere inside a semi-enclosed plastic enclosure surrounding the spray plume. The vapors of acetone, acetonitrile, ethyl acetate, methanol, and water were investigated. Vapor dependent effects were observed with respect to changes in protein charge state distributions and signal intensities. With ethyl acetate vapor addition, the signal intensities of all proteins investigated were significantly increased, including proteins larger than 25 kDa such as carbonic anhydrase II and bovine serum albumin.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>31758521</pmid><doi>10.1007/s13361-019-02345-w</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0001-6912-7446</orcidid></addata></record> |
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| subjects | Acetates - chemistry Acetone Acetone - chemistry Acetonitrile Acetonitriles - chemistry Additives Alkali metals Analytical Chemistry Animals Bioinformatics Biotechnology Carbonic anhydrase Carbonic Anhydrase II - analysis Cattle Charge distribution Chemistry Chemistry and Materials Science Cytochromes c - analysis Equipment Design Ethyl acetate Horses Ions Mass spectrometry Methanol - chemistry Organic Chemistry Proteins Proteins - analysis Proteomics Serum albumin Serum Albumin, Bovine - analysis Short Communication Solvents Solvents - chemistry Spectrometry, Mass, Electrospray Ionization - instrumentation Spectrometry, Mass, Electrospray Ionization - methods Vapors Volatilization Water - chemistry |
| title | The Addition of Polar Organic Solvent Vapors During the Analysis of Proteins by DESI-MS |
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