Transcriptomic analysis reveals key genes related to antioxidant mechanisms of Hylocereus undatus quality improvement by trypsin during storage
It has been revealed in our previous studies that trypsin scavenges superoxide anions. In the current study, the mechanisms of storage quality improvement by trypsin were evaluated in H. undatus. Strikingly, the improvement is due not to its antibacterial or antifungal activity but to its superoxide...
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| Veröffentlicht in: | Food & function 2019-12, Vol.10 (12), p.8116-8128 |
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| creator | Li, Xin Liu, Xueru Yin, Yong Yu, Huichun Zhang, Min Jing, Haonan Ma, Yingchao Xiong, Xianlang Pang, Xinyue |
| description | It has been revealed in our previous studies that trypsin scavenges superoxide anions. In the current study, the mechanisms of storage quality improvement by trypsin were evaluated in H. undatus. Strikingly, the improvement is due not to its antibacterial or antifungal activity but to its superoxide scavenging activity. Moreover, trypsin significantly decreased the levels of ROS, cell permeability and membrane lipid peroxidation. The activities of major antioxidant enzymes were significantly improved by trypsin treatment. Transcriptome profiles of H. undatus treated with trypsin revealed the pathways and regulatory mechanisms of antioxidant genes up or down-regulated following trypsin treatment by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) enrichment analyses. The results of protein-protein interaction networks indicated that CAT is the key among the enzymes of the complicated antioxidant system. In addition, the current results showed that the synergistic effect of trypsin with antioxidant enzymes can regulate the levels of endogenous active oxygen species, reduce malondialdehyde content, improve cell membrane integrity, alleviate cell damage and delay fruit ageing. |
| doi_str_mv | 10.1039/c9fo00809h |
| format | Article |
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In the current study, the mechanisms of storage quality improvement by trypsin were evaluated in H. undatus. Strikingly, the improvement is due not to its antibacterial or antifungal activity but to its superoxide scavenging activity. Moreover, trypsin significantly decreased the levels of ROS, cell permeability and membrane lipid peroxidation. The activities of major antioxidant enzymes were significantly improved by trypsin treatment. Transcriptome profiles of H. undatus treated with trypsin revealed the pathways and regulatory mechanisms of antioxidant genes up or down-regulated following trypsin treatment by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) enrichment analyses. The results of protein-protein interaction networks indicated that CAT is the key among the enzymes of the complicated antioxidant system. In addition, the current results showed that the synergistic effect of trypsin with antioxidant enzymes can regulate the levels of endogenous active oxygen species, reduce malondialdehyde content, improve cell membrane integrity, alleviate cell damage and delay fruit ageing.</description><identifier>ISSN: 2042-6496</identifier><identifier>EISSN: 2042-650X</identifier><identifier>DOI: 10.1039/c9fo00809h</identifier><identifier>PMID: 31746888</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Aging ; Anions ; Antifungal activity ; Antioxidants ; Antioxidants - analysis ; Antioxidants - metabolism ; Cactaceae - chemistry ; Cactaceae - genetics ; Cactaceae - metabolism ; Cell membranes ; Cell permeability ; Encyclopedias ; Enzymes ; Food Preservation - methods ; Food Storage ; Fruit - chemistry ; Fruit - genetics ; Fruit - metabolism ; Fungicides ; Gene expression ; Gene Expression Profiling ; Gene Ontology ; Genes ; Genomes ; Lipid peroxidation ; Lipids ; Malondialdehyde ; Malondialdehyde - analysis ; Malondialdehyde - metabolism ; Membrane permeability ; Peroxidation ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Proteins ; Quality control ; Reactive Oxygen Species - analysis ; Reactive Oxygen Species - metabolism ; Regulatory mechanisms (biology) ; Scavenging ; Storage ; Superoxide ; Superoxide anions ; Synergistic effect ; Transcriptome ; Trypsin ; Trypsin - chemistry</subject><ispartof>Food & function, 2019-12, Vol.10 (12), p.8116-8128</ispartof><rights>Copyright Royal Society of Chemistry 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-66a5251bbc53e22be3b0550bbf88f9e7d2e3f7c0da7e8b5b2a223016f567edf83</citedby><cites>FETCH-LOGICAL-c356t-66a5251bbc53e22be3b0550bbf88f9e7d2e3f7c0da7e8b5b2a223016f567edf83</cites><orcidid>0000-0002-5976-2988</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31746888$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Xin</creatorcontrib><creatorcontrib>Liu, Xueru</creatorcontrib><creatorcontrib>Yin, Yong</creatorcontrib><creatorcontrib>Yu, Huichun</creatorcontrib><creatorcontrib>Zhang, Min</creatorcontrib><creatorcontrib>Jing, Haonan</creatorcontrib><creatorcontrib>Ma, Yingchao</creatorcontrib><creatorcontrib>Xiong, Xianlang</creatorcontrib><creatorcontrib>Pang, Xinyue</creatorcontrib><title>Transcriptomic analysis reveals key genes related to antioxidant mechanisms of Hylocereus undatus quality improvement by trypsin during storage</title><title>Food & function</title><addtitle>Food Funct</addtitle><description>It has been revealed in our previous studies that trypsin scavenges superoxide anions. In the current study, the mechanisms of storage quality improvement by trypsin were evaluated in H. undatus. Strikingly, the improvement is due not to its antibacterial or antifungal activity but to its superoxide scavenging activity. Moreover, trypsin significantly decreased the levels of ROS, cell permeability and membrane lipid peroxidation. The activities of major antioxidant enzymes were significantly improved by trypsin treatment. Transcriptome profiles of H. undatus treated with trypsin revealed the pathways and regulatory mechanisms of antioxidant genes up or down-regulated following trypsin treatment by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) enrichment analyses. The results of protein-protein interaction networks indicated that CAT is the key among the enzymes of the complicated antioxidant system. In addition, the current results showed that the synergistic effect of trypsin with antioxidant enzymes can regulate the levels of endogenous active oxygen species, reduce malondialdehyde content, improve cell membrane integrity, alleviate cell damage and delay fruit ageing.</description><subject>Aging</subject><subject>Anions</subject><subject>Antifungal activity</subject><subject>Antioxidants</subject><subject>Antioxidants - analysis</subject><subject>Antioxidants - metabolism</subject><subject>Cactaceae - chemistry</subject><subject>Cactaceae - genetics</subject><subject>Cactaceae - metabolism</subject><subject>Cell membranes</subject><subject>Cell permeability</subject><subject>Encyclopedias</subject><subject>Enzymes</subject><subject>Food Preservation - methods</subject><subject>Food Storage</subject><subject>Fruit - chemistry</subject><subject>Fruit - genetics</subject><subject>Fruit - metabolism</subject><subject>Fungicides</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Ontology</subject><subject>Genes</subject><subject>Genomes</subject><subject>Lipid peroxidation</subject><subject>Lipids</subject><subject>Malondialdehyde</subject><subject>Malondialdehyde - analysis</subject><subject>Malondialdehyde - metabolism</subject><subject>Membrane permeability</subject><subject>Peroxidation</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Proteins</subject><subject>Quality control</subject><subject>Reactive Oxygen Species - analysis</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Regulatory mechanisms (biology)</subject><subject>Scavenging</subject><subject>Storage</subject><subject>Superoxide</subject><subject>Superoxide anions</subject><subject>Synergistic effect</subject><subject>Transcriptome</subject><subject>Trypsin</subject><subject>Trypsin - chemistry</subject><issn>2042-6496</issn><issn>2042-650X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU1r3DAQhkVoyS5pLvkBRdBLKWwrS5YsHcuSdAOBvaSQm5Hk0Uapbe1Kcoh_Rf5ytc3HoXN5h-GZD-ZF6KIi3yvC1A-rXCBEEnV_gpaU1HQlOLn78JbXSizQeUoPpARTSip5ihasamohpVyi59uox2Sj3-cweIv1qPs5-YQjPILuE_4DM97BCMdKrzN0OIdCZR-efFcUD2Dv9ejTkHBweDP3wUKEKeFp7HQueph07_OM_bCP4REGKE1mxjnO--RH3E3Rjzuccoh6B5_QR1fWwvmrnqHfV5e3683qZvvrev3zZmUZF3klhOaUV8ZYzoBSA8wQzokxTkqnoOkoMNdY0ukGpOGGakoZqYTjooHOSXaGvr7MLTcdJki5HXyy0Pd6hDCllrJK1IwISQr65T_0IUyx_OlI0VoS0XBaqG8vlI0hpQiu3Uc_6Di3FWmPTrVrdbX959SmwJ9fR05mgO4dffOF_QVwDpJU</recordid><startdate>20191211</startdate><enddate>20191211</enddate><creator>Li, Xin</creator><creator>Liu, Xueru</creator><creator>Yin, Yong</creator><creator>Yu, Huichun</creator><creator>Zhang, Min</creator><creator>Jing, Haonan</creator><creator>Ma, Yingchao</creator><creator>Xiong, Xianlang</creator><creator>Pang, Xinyue</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7T7</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5976-2988</orcidid></search><sort><creationdate>20191211</creationdate><title>Transcriptomic analysis reveals key genes related to antioxidant mechanisms of Hylocereus undatus quality improvement by trypsin during storage</title><author>Li, Xin ; Liu, Xueru ; Yin, Yong ; Yu, Huichun ; Zhang, Min ; Jing, Haonan ; Ma, Yingchao ; Xiong, Xianlang ; Pang, Xinyue</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-66a5251bbc53e22be3b0550bbf88f9e7d2e3f7c0da7e8b5b2a223016f567edf83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Aging</topic><topic>Anions</topic><topic>Antifungal activity</topic><topic>Antioxidants</topic><topic>Antioxidants - analysis</topic><topic>Antioxidants - metabolism</topic><topic>Cactaceae - chemistry</topic><topic>Cactaceae - genetics</topic><topic>Cactaceae - metabolism</topic><topic>Cell membranes</topic><topic>Cell permeability</topic><topic>Encyclopedias</topic><topic>Enzymes</topic><topic>Food Preservation - methods</topic><topic>Food Storage</topic><topic>Fruit - chemistry</topic><topic>Fruit - genetics</topic><topic>Fruit - metabolism</topic><topic>Fungicides</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Gene Ontology</topic><topic>Genes</topic><topic>Genomes</topic><topic>Lipid peroxidation</topic><topic>Lipids</topic><topic>Malondialdehyde</topic><topic>Malondialdehyde - analysis</topic><topic>Malondialdehyde - metabolism</topic><topic>Membrane permeability</topic><topic>Peroxidation</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Proteins</topic><topic>Quality control</topic><topic>Reactive Oxygen Species - analysis</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Regulatory mechanisms (biology)</topic><topic>Scavenging</topic><topic>Storage</topic><topic>Superoxide</topic><topic>Superoxide anions</topic><topic>Synergistic effect</topic><topic>Transcriptome</topic><topic>Trypsin</topic><topic>Trypsin - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Xin</creatorcontrib><creatorcontrib>Liu, Xueru</creatorcontrib><creatorcontrib>Yin, Yong</creatorcontrib><creatorcontrib>Yu, Huichun</creatorcontrib><creatorcontrib>Zhang, Min</creatorcontrib><creatorcontrib>Jing, Haonan</creatorcontrib><creatorcontrib>Ma, Yingchao</creatorcontrib><creatorcontrib>Xiong, Xianlang</creatorcontrib><creatorcontrib>Pang, Xinyue</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Food & function</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xin</au><au>Liu, Xueru</au><au>Yin, Yong</au><au>Yu, Huichun</au><au>Zhang, Min</au><au>Jing, Haonan</au><au>Ma, Yingchao</au><au>Xiong, Xianlang</au><au>Pang, Xinyue</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptomic analysis reveals key genes related to antioxidant mechanisms of Hylocereus undatus quality improvement by trypsin during storage</atitle><jtitle>Food & function</jtitle><addtitle>Food Funct</addtitle><date>2019-12-11</date><risdate>2019</risdate><volume>10</volume><issue>12</issue><spage>8116</spage><epage>8128</epage><pages>8116-8128</pages><issn>2042-6496</issn><eissn>2042-650X</eissn><abstract>It has been revealed in our previous studies that trypsin scavenges superoxide anions. In the current study, the mechanisms of storage quality improvement by trypsin were evaluated in H. undatus. Strikingly, the improvement is due not to its antibacterial or antifungal activity but to its superoxide scavenging activity. Moreover, trypsin significantly decreased the levels of ROS, cell permeability and membrane lipid peroxidation. The activities of major antioxidant enzymes were significantly improved by trypsin treatment. Transcriptome profiles of H. undatus treated with trypsin revealed the pathways and regulatory mechanisms of antioxidant genes up or down-regulated following trypsin treatment by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) enrichment analyses. The results of protein-protein interaction networks indicated that CAT is the key among the enzymes of the complicated antioxidant system. In addition, the current results showed that the synergistic effect of trypsin with antioxidant enzymes can regulate the levels of endogenous active oxygen species, reduce malondialdehyde content, improve cell membrane integrity, alleviate cell damage and delay fruit ageing.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>31746888</pmid><doi>10.1039/c9fo00809h</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-5976-2988</orcidid></addata></record> |
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| subjects | Aging Anions Antifungal activity Antioxidants Antioxidants - analysis Antioxidants - metabolism Cactaceae - chemistry Cactaceae - genetics Cactaceae - metabolism Cell membranes Cell permeability Encyclopedias Enzymes Food Preservation - methods Food Storage Fruit - chemistry Fruit - genetics Fruit - metabolism Fungicides Gene expression Gene Expression Profiling Gene Ontology Genes Genomes Lipid peroxidation Lipids Malondialdehyde Malondialdehyde - analysis Malondialdehyde - metabolism Membrane permeability Peroxidation Plant Proteins - genetics Plant Proteins - metabolism Proteins Quality control Reactive Oxygen Species - analysis Reactive Oxygen Species - metabolism Regulatory mechanisms (biology) Scavenging Storage Superoxide Superoxide anions Synergistic effect Transcriptome Trypsin Trypsin - chemistry |
| title | Transcriptomic analysis reveals key genes related to antioxidant mechanisms of Hylocereus undatus quality improvement by trypsin during storage |
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