Neuroprotective effect of Convolvulus pluricaulis Choisy in oxidative stress model of cerebral ischemia reperfusion injury and assessment of MAP2 in rats

Convolvulus pluricaulis Choisy commonly known as Shankhapushpi, is traditionally prescribed for nerve debility, loss of memory, epilepsy and as nervine tonic. Plant also proved to have diverse pharmacological activity but the neuroprotection in ischemic stroke were not found. To investigate the effe...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of ethnopharmacology 2020-03, Vol.249 (NA), p.112393-112393, Article 112393
Hauptverfasser: Shalavadi, Mallappa H., Chandrashekhar, V.M., Muchchandi, I.S.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 112393
container_issue NA
container_start_page 112393
container_title Journal of ethnopharmacology
container_volume 249
creator Shalavadi, Mallappa H.
Chandrashekhar, V.M.
Muchchandi, I.S.
description Convolvulus pluricaulis Choisy commonly known as Shankhapushpi, is traditionally prescribed for nerve debility, loss of memory, epilepsy and as nervine tonic. Plant also proved to have diverse pharmacological activity but the neuroprotection in ischemic stroke were not found. To investigate the effect of Convolvulus pluricaulis against bilateral common carotid artery (BCCA) occlusion induced cerebral ischemic reperfusion injury. The neuroprotective activity of Convolvulus pluricaulis against bilateral common carotid artery (BCCA) occlusion induced cerebral ischemic reperfusion (I/S) injury. Sprague-Dawley rats of either sex (200–250 g) were divided into nine groups of 8 rats each. Sham and control group, saline treated 10 ml/kg orally. Third group treated with Quercetin 25 mg/kg orally and fourth to ninth groups treated with chloroform and ethanol extract of Convolvulus pluricaulis 100, 200, and 400 mg/kg (p.o.) respectively. Control, Quercetin and extract treated groups underwent 30 min BCCA occlusion and 24 h reperfusion on 10th day but sham underwent same surgery without BCCA occlusion and 24 h reperfusion on 10th day. The antioxidant enzymatic and non-enzymatic levels were estimated by UV spectroscopic method and cerebral infarction area, Blood brain barrier disruption, microtubule-associated protein 2 immunohistochemical and histopathological studies were carried out. The results of the study indicate that the chloroform and ethanol extract of Convolvulus pluricaulis showed neuroprotective activity by a significant decrease in lipid peroxidation (p 
doi_str_mv 10.1016/j.jep.2019.112393
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2316428654</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378874118335256</els_id><sourcerecordid>2316428654</sourcerecordid><originalsourceid>FETCH-LOGICAL-c386t-c6f379a5f25baffb25768d0101aefec25e825aea24f691abe570257341befe433</originalsourceid><addsrcrecordid>eNqFkctu1DAUhi0EotPCA7BBXrLJ4FviRKyqUblILe0C1paTHKuOkjj4xKPOo_C29XQKS1jZkr_vt31-Qt5xtuWMVx-H7QDLVjDebDkXspEvyIbXWhS61PIl2TCp66LWip-Rc8SBMaa5Yq_JmeRaSV2pDfn9HVIMSwwrdKvfAwXn8o4GR3dh3odxn8aEdBlT9J1No0e6uw8eD9TPNDz43j5ZuEZApFPoYTy6HURoox2px-4eJm9phAWiS-jDnNUhxQO1c08tYhYnmJ-uvLm8E8fgaFd8Q145OyK8fV4vyM_PVz92X4vr2y_fdpfXRSfrai26yknd2NKJsrXOtaLUVd2zPB8L-SeihFqUFqxQrmq4baHULDNS8TafKykvyIdTbh7CrwS4mik_GsbRzhASGqGYbiqlpfg_KnmlRF2VKqP8hHYxIEZwZol-svFgODPH8sxgcnnmWJ45lZed98_xqZ2g_2v8aSsDn04A5HnsPUSDnYe5g97HXJrpg_9H_CMo_q2i</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2316428654</pqid></control><display><type>article</type><title>Neuroprotective effect of Convolvulus pluricaulis Choisy in oxidative stress model of cerebral ischemia reperfusion injury and assessment of MAP2 in rats</title><source>Elsevier ScienceDirect Journals Complete</source><creator>Shalavadi, Mallappa H. ; Chandrashekhar, V.M. ; Muchchandi, I.S.</creator><creatorcontrib>Shalavadi, Mallappa H. ; Chandrashekhar, V.M. ; Muchchandi, I.S.</creatorcontrib><description><![CDATA[Convolvulus pluricaulis Choisy commonly known as Shankhapushpi, is traditionally prescribed for nerve debility, loss of memory, epilepsy and as nervine tonic. Plant also proved to have diverse pharmacological activity but the neuroprotection in ischemic stroke were not found. To investigate the effect of Convolvulus pluricaulis against bilateral common carotid artery (BCCA) occlusion induced cerebral ischemic reperfusion injury. The neuroprotective activity of Convolvulus pluricaulis against bilateral common carotid artery (BCCA) occlusion induced cerebral ischemic reperfusion (I/S) injury. Sprague-Dawley rats of either sex (200–250 g) were divided into nine groups of 8 rats each. Sham and control group, saline treated 10 ml/kg orally. Third group treated with Quercetin 25 mg/kg orally and fourth to ninth groups treated with chloroform and ethanol extract of Convolvulus pluricaulis 100, 200, and 400 mg/kg (p.o.) respectively. Control, Quercetin and extract treated groups underwent 30 min BCCA occlusion and 24 h reperfusion on 10th day but sham underwent same surgery without BCCA occlusion and 24 h reperfusion on 10th day. The antioxidant enzymatic and non-enzymatic levels were estimated by UV spectroscopic method and cerebral infarction area, Blood brain barrier disruption, microtubule-associated protein 2 immunohistochemical and histopathological studies were carried out. The results of the study indicate that the chloroform and ethanol extract of Convolvulus pluricaulis showed neuroprotective activity by a significant decrease in lipid peroxidation (p < 0.001) and an increase in superoxide dismutase (p < 0.01, p < 0.001), catalase (p < 0.01, p < 0.001), glutathione (p < 0.001), and total thiol (p < 0.001) levels in extract-treated groups as compared to control group. Measurement of cerebral infarction area, blood brain barrier disruption, microtubule-associated protein 2 immunohistochemical and histopathological studies further supported the protective effect of the extract. Present study revile that Convolvulus pluricaulis has potent neuroprotection against bilateral common carotid artery (BCCA) occlusion induced cerebral ischemic reperfusion injury. [Display omitted]]]></description><identifier>ISSN: 0378-8741</identifier><identifier>EISSN: 1872-7573</identifier><identifier>DOI: 10.1016/j.jep.2019.112393</identifier><identifier>PMID: 31743764</identifier><language>eng</language><publisher>Ireland: Elsevier B.V</publisher><subject>Antioxidant ; Extract ; Flavonoids ; Free radical scavenger ; Ischemia ; MAP2 ; Microtubule ; Stroke</subject><ispartof>Journal of ethnopharmacology, 2020-03, Vol.249 (NA), p.112393-112393, Article 112393</ispartof><rights>2019 Elsevier B.V.</rights><rights>Copyright © 2019 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-c6f379a5f25baffb25768d0101aefec25e825aea24f691abe570257341befe433</citedby><cites>FETCH-LOGICAL-c386t-c6f379a5f25baffb25768d0101aefec25e825aea24f691abe570257341befe433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378874118335256$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31743764$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shalavadi, Mallappa H.</creatorcontrib><creatorcontrib>Chandrashekhar, V.M.</creatorcontrib><creatorcontrib>Muchchandi, I.S.</creatorcontrib><title>Neuroprotective effect of Convolvulus pluricaulis Choisy in oxidative stress model of cerebral ischemia reperfusion injury and assessment of MAP2 in rats</title><title>Journal of ethnopharmacology</title><addtitle>J Ethnopharmacol</addtitle><description><![CDATA[Convolvulus pluricaulis Choisy commonly known as Shankhapushpi, is traditionally prescribed for nerve debility, loss of memory, epilepsy and as nervine tonic. Plant also proved to have diverse pharmacological activity but the neuroprotection in ischemic stroke were not found. To investigate the effect of Convolvulus pluricaulis against bilateral common carotid artery (BCCA) occlusion induced cerebral ischemic reperfusion injury. The neuroprotective activity of Convolvulus pluricaulis against bilateral common carotid artery (BCCA) occlusion induced cerebral ischemic reperfusion (I/S) injury. Sprague-Dawley rats of either sex (200–250 g) were divided into nine groups of 8 rats each. Sham and control group, saline treated 10 ml/kg orally. Third group treated with Quercetin 25 mg/kg orally and fourth to ninth groups treated with chloroform and ethanol extract of Convolvulus pluricaulis 100, 200, and 400 mg/kg (p.o.) respectively. Control, Quercetin and extract treated groups underwent 30 min BCCA occlusion and 24 h reperfusion on 10th day but sham underwent same surgery without BCCA occlusion and 24 h reperfusion on 10th day. The antioxidant enzymatic and non-enzymatic levels were estimated by UV spectroscopic method and cerebral infarction area, Blood brain barrier disruption, microtubule-associated protein 2 immunohistochemical and histopathological studies were carried out. The results of the study indicate that the chloroform and ethanol extract of Convolvulus pluricaulis showed neuroprotective activity by a significant decrease in lipid peroxidation (p < 0.001) and an increase in superoxide dismutase (p < 0.01, p < 0.001), catalase (p < 0.01, p < 0.001), glutathione (p < 0.001), and total thiol (p < 0.001) levels in extract-treated groups as compared to control group. Measurement of cerebral infarction area, blood brain barrier disruption, microtubule-associated protein 2 immunohistochemical and histopathological studies further supported the protective effect of the extract. Present study revile that Convolvulus pluricaulis has potent neuroprotection against bilateral common carotid artery (BCCA) occlusion induced cerebral ischemic reperfusion injury. [Display omitted]]]></description><subject>Antioxidant</subject><subject>Extract</subject><subject>Flavonoids</subject><subject>Free radical scavenger</subject><subject>Ischemia</subject><subject>MAP2</subject><subject>Microtubule</subject><subject>Stroke</subject><issn>0378-8741</issn><issn>1872-7573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNqFkctu1DAUhi0EotPCA7BBXrLJ4FviRKyqUblILe0C1paTHKuOkjj4xKPOo_C29XQKS1jZkr_vt31-Qt5xtuWMVx-H7QDLVjDebDkXspEvyIbXWhS61PIl2TCp66LWip-Rc8SBMaa5Yq_JmeRaSV2pDfn9HVIMSwwrdKvfAwXn8o4GR3dh3odxn8aEdBlT9J1No0e6uw8eD9TPNDz43j5ZuEZApFPoYTy6HURoox2px-4eJm9phAWiS-jDnNUhxQO1c08tYhYnmJ-uvLm8E8fgaFd8Q145OyK8fV4vyM_PVz92X4vr2y_fdpfXRSfrai26yknd2NKJsrXOtaLUVd2zPB8L-SeihFqUFqxQrmq4baHULDNS8TafKykvyIdTbh7CrwS4mik_GsbRzhASGqGYbiqlpfg_KnmlRF2VKqP8hHYxIEZwZol-svFgODPH8sxgcnnmWJ45lZed98_xqZ2g_2v8aSsDn04A5HnsPUSDnYe5g97HXJrpg_9H_CMo_q2i</recordid><startdate>20200301</startdate><enddate>20200301</enddate><creator>Shalavadi, Mallappa H.</creator><creator>Chandrashekhar, V.M.</creator><creator>Muchchandi, I.S.</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20200301</creationdate><title>Neuroprotective effect of Convolvulus pluricaulis Choisy in oxidative stress model of cerebral ischemia reperfusion injury and assessment of MAP2 in rats</title><author>Shalavadi, Mallappa H. ; Chandrashekhar, V.M. ; Muchchandi, I.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-c6f379a5f25baffb25768d0101aefec25e825aea24f691abe570257341befe433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Antioxidant</topic><topic>Extract</topic><topic>Flavonoids</topic><topic>Free radical scavenger</topic><topic>Ischemia</topic><topic>MAP2</topic><topic>Microtubule</topic><topic>Stroke</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shalavadi, Mallappa H.</creatorcontrib><creatorcontrib>Chandrashekhar, V.M.</creatorcontrib><creatorcontrib>Muchchandi, I.S.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of ethnopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shalavadi, Mallappa H.</au><au>Chandrashekhar, V.M.</au><au>Muchchandi, I.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neuroprotective effect of Convolvulus pluricaulis Choisy in oxidative stress model of cerebral ischemia reperfusion injury and assessment of MAP2 in rats</atitle><jtitle>Journal of ethnopharmacology</jtitle><addtitle>J Ethnopharmacol</addtitle><date>2020-03-01</date><risdate>2020</risdate><volume>249</volume><issue>NA</issue><spage>112393</spage><epage>112393</epage><pages>112393-112393</pages><artnum>112393</artnum><issn>0378-8741</issn><eissn>1872-7573</eissn><abstract><![CDATA[Convolvulus pluricaulis Choisy commonly known as Shankhapushpi, is traditionally prescribed for nerve debility, loss of memory, epilepsy and as nervine tonic. Plant also proved to have diverse pharmacological activity but the neuroprotection in ischemic stroke were not found. To investigate the effect of Convolvulus pluricaulis against bilateral common carotid artery (BCCA) occlusion induced cerebral ischemic reperfusion injury. The neuroprotective activity of Convolvulus pluricaulis against bilateral common carotid artery (BCCA) occlusion induced cerebral ischemic reperfusion (I/S) injury. Sprague-Dawley rats of either sex (200–250 g) were divided into nine groups of 8 rats each. Sham and control group, saline treated 10 ml/kg orally. Third group treated with Quercetin 25 mg/kg orally and fourth to ninth groups treated with chloroform and ethanol extract of Convolvulus pluricaulis 100, 200, and 400 mg/kg (p.o.) respectively. Control, Quercetin and extract treated groups underwent 30 min BCCA occlusion and 24 h reperfusion on 10th day but sham underwent same surgery without BCCA occlusion and 24 h reperfusion on 10th day. The antioxidant enzymatic and non-enzymatic levels were estimated by UV spectroscopic method and cerebral infarction area, Blood brain barrier disruption, microtubule-associated protein 2 immunohistochemical and histopathological studies were carried out. The results of the study indicate that the chloroform and ethanol extract of Convolvulus pluricaulis showed neuroprotective activity by a significant decrease in lipid peroxidation (p < 0.001) and an increase in superoxide dismutase (p < 0.01, p < 0.001), catalase (p < 0.01, p < 0.001), glutathione (p < 0.001), and total thiol (p < 0.001) levels in extract-treated groups as compared to control group. Measurement of cerebral infarction area, blood brain barrier disruption, microtubule-associated protein 2 immunohistochemical and histopathological studies further supported the protective effect of the extract. Present study revile that Convolvulus pluricaulis has potent neuroprotection against bilateral common carotid artery (BCCA) occlusion induced cerebral ischemic reperfusion injury. [Display omitted]]]></abstract><cop>Ireland</cop><pub>Elsevier B.V</pub><pmid>31743764</pmid><doi>10.1016/j.jep.2019.112393</doi><tpages>1</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0378-8741
ispartof Journal of ethnopharmacology, 2020-03, Vol.249 (NA), p.112393-112393, Article 112393
issn 0378-8741
1872-7573
language eng
recordid cdi_proquest_miscellaneous_2316428654
source Elsevier ScienceDirect Journals Complete
subjects Antioxidant
Extract
Flavonoids
Free radical scavenger
Ischemia
MAP2
Microtubule
Stroke
title Neuroprotective effect of Convolvulus pluricaulis Choisy in oxidative stress model of cerebral ischemia reperfusion injury and assessment of MAP2 in rats
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-14T11%3A49%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Neuroprotective%20effect%20of%20Convolvulus%20pluricaulis%20Choisy%20in%20oxidative%20stress%20model%20of%20cerebral%20ischemia%20reperfusion%20injury%20and%20assessment%20of%20MAP2%20in%20rats&rft.jtitle=Journal%20of%20ethnopharmacology&rft.au=Shalavadi,%20Mallappa%20H.&rft.date=2020-03-01&rft.volume=249&rft.issue=NA&rft.spage=112393&rft.epage=112393&rft.pages=112393-112393&rft.artnum=112393&rft.issn=0378-8741&rft.eissn=1872-7573&rft_id=info:doi/10.1016/j.jep.2019.112393&rft_dat=%3Cproquest_cross%3E2316428654%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2316428654&rft_id=info:pmid/31743764&rft_els_id=S0378874118335256&rfr_iscdi=true