TMTpro: Design, Synthesis, and Initial Evaluation of a Proline-Based Isobaric 16-Plex Tandem Mass Tag Reagent Set
The design and synthesis of a proline-based reporter isobaric Tandem Mass Tag structure (TMTpro) is presented. An analysis is made of the performance of the new TMTpro tags in comparison with the current commercially available dimethylpiperidine-reporter-based TMT10/11 reagents. The new reporter str...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2019-12, Vol.91 (24), p.15941-15950 |
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| creator | Thompson, Andrew Wölmer, Nikolai Koncarevic, Sasa Selzer, Stefan Böhm, Gitte Legner, Harald Schmid, Peter Kienle, Stefan Penning, Petra Höhle, Claudia Berfelde, Antje Martinez-Pinna, Roxana Farztdinov, Vadim Jung, Stephan Kuhn, Karsten Pike, Ian |
| description | The design and synthesis of a proline-based reporter isobaric Tandem Mass Tag structure (TMTpro) is presented. An analysis is made of the performance of the new TMTpro tags in comparison with the current commercially available dimethylpiperidine-reporter-based TMT10/11 reagents. The new reporter structure provides a set of 16 tags for use with resolution of 6.3 mDa mass differences in high resolution mass spectrometers and a set of 9 reagents with 1 Da spacing between reporter ions for single dalton analysis using 9 heavy nuclei per tag. We show similar performance in terms of peptide identification rates and quantification between the TMTpro 16-plex and TMT10/11-plex reagents. We also demonstrate the suitability of the TMTpro reagents for phosphopeptide analysis. The ability to pool 16 samples reduces the overall amount of sample required for each channel, and we anticipate that TMTpro reagents will be a useful enhancement for any protocol that benefits from sample pooling and should reduce missing data. |
| doi_str_mv | 10.1021/acs.analchem.9b04474 |
| format | Article |
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An analysis is made of the performance of the new TMTpro tags in comparison with the current commercially available dimethylpiperidine-reporter-based TMT10/11 reagents. The new reporter structure provides a set of 16 tags for use with resolution of 6.3 mDa mass differences in high resolution mass spectrometers and a set of 9 reagents with 1 Da spacing between reporter ions for single dalton analysis using 9 heavy nuclei per tag. We show similar performance in terms of peptide identification rates and quantification between the TMTpro 16-plex and TMT10/11-plex reagents. We also demonstrate the suitability of the TMTpro reagents for phosphopeptide analysis. 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The ability to pool 16 samples reduces the overall amount of sample required for each channel, and we anticipate that TMTpro reagents will be a useful enhancement for any protocol that benefits from sample pooling and should reduce missing data.</description><subject>Analytical chemistry</subject><subject>Chemistry</subject><subject>Heavy nuclei</subject><subject>Mass spectrometers</subject><subject>Missing data</subject><subject>Proline</subject><subject>Reagents</subject><subject>Spectrometers</subject><subject>Synthesis</subject><subject>Tags</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kcFO3DAQhq2qCLbAG1SVpV56INsZO4mT3iilLRKoCLbnaGJPlqDEgTipytvXq1049MDJc_j-f-T5hHiPsERQ-JlsWJKnzt5xvyxrSFOTvhELzBQkeVGot2IBADpRBuBAvAvhHgARMN8XBxqNLjI0C_G4ulo9jMMX-Y1Du_Yn8vbJT3dxDieSvJMXvp1a6uT5H-pmmtrBy6GRJK_HoWs9J18pcKTCUNPYWol5ct3xX7mKWe7lFYUQ57W8YVqzn-QtT0dir6Eu8PHuPRS_v5-vzn4ml79-XJydXiaUpmpK2KjUsYaCtC4zZxExq8uaTA0uKzBzuSutKwvloLHkDJdosGjq3IFtgJw-FJ-2vfF7jzOHqerbYLnryPMwh0ppzEqjtdIR_fgfej_MY7zthtIQr53neaTSLWXHIYSRm-phbHsanyqEaqOkikqqZyXVTkmMfdiVz3XP7iX07CACsAU28ZfFr3b-A7tYmX8</recordid><startdate>20191217</startdate><enddate>20191217</enddate><creator>Thompson, Andrew</creator><creator>Wölmer, Nikolai</creator><creator>Koncarevic, Sasa</creator><creator>Selzer, Stefan</creator><creator>Böhm, Gitte</creator><creator>Legner, Harald</creator><creator>Schmid, Peter</creator><creator>Kienle, Stefan</creator><creator>Penning, Petra</creator><creator>Höhle, Claudia</creator><creator>Berfelde, Antje</creator><creator>Martinez-Pinna, Roxana</creator><creator>Farztdinov, Vadim</creator><creator>Jung, Stephan</creator><creator>Kuhn, Karsten</creator><creator>Pike, Ian</creator><general>American Chemical Society</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7786-5203</orcidid></search><sort><creationdate>20191217</creationdate><title>TMTpro: Design, Synthesis, and Initial Evaluation of a Proline-Based Isobaric 16-Plex Tandem Mass Tag Reagent Set</title><author>Thompson, Andrew ; Wölmer, Nikolai ; Koncarevic, Sasa ; Selzer, Stefan ; Böhm, Gitte ; Legner, Harald ; Schmid, Peter ; Kienle, Stefan ; Penning, Petra ; Höhle, Claudia ; Berfelde, Antje ; Martinez-Pinna, Roxana ; Farztdinov, Vadim ; Jung, Stephan ; Kuhn, Karsten ; Pike, Ian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a442t-e724de308a3395dc1115b9ba7b0d5815d6d9cd982d0fcad7e91718fb6d0cf0ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Analytical chemistry</topic><topic>Chemistry</topic><topic>Heavy nuclei</topic><topic>Mass spectrometers</topic><topic>Missing data</topic><topic>Proline</topic><topic>Reagents</topic><topic>Spectrometers</topic><topic>Synthesis</topic><topic>Tags</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thompson, Andrew</creatorcontrib><creatorcontrib>Wölmer, Nikolai</creatorcontrib><creatorcontrib>Koncarevic, Sasa</creatorcontrib><creatorcontrib>Selzer, Stefan</creatorcontrib><creatorcontrib>Böhm, Gitte</creatorcontrib><creatorcontrib>Legner, Harald</creatorcontrib><creatorcontrib>Schmid, Peter</creatorcontrib><creatorcontrib>Kienle, Stefan</creatorcontrib><creatorcontrib>Penning, Petra</creatorcontrib><creatorcontrib>Höhle, Claudia</creatorcontrib><creatorcontrib>Berfelde, Antje</creatorcontrib><creatorcontrib>Martinez-Pinna, Roxana</creatorcontrib><creatorcontrib>Farztdinov, Vadim</creatorcontrib><creatorcontrib>Jung, Stephan</creatorcontrib><creatorcontrib>Kuhn, Karsten</creatorcontrib><creatorcontrib>Pike, Ian</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thompson, Andrew</au><au>Wölmer, Nikolai</au><au>Koncarevic, Sasa</au><au>Selzer, Stefan</au><au>Böhm, Gitte</au><au>Legner, Harald</au><au>Schmid, Peter</au><au>Kienle, Stefan</au><au>Penning, Petra</au><au>Höhle, Claudia</au><au>Berfelde, Antje</au><au>Martinez-Pinna, Roxana</au><au>Farztdinov, Vadim</au><au>Jung, Stephan</au><au>Kuhn, Karsten</au><au>Pike, Ian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TMTpro: Design, Synthesis, and Initial Evaluation of a Proline-Based Isobaric 16-Plex Tandem Mass Tag Reagent Set</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. 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| subjects | Analytical chemistry Chemistry Heavy nuclei Mass spectrometers Missing data Proline Reagents Spectrometers Synthesis Tags |
| title | TMTpro: Design, Synthesis, and Initial Evaluation of a Proline-Based Isobaric 16-Plex Tandem Mass Tag Reagent Set |
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