Mito-Tempo alleviates cryodamage by regulating intracellular oxidative metabolism in spermatozoa from asthenozoospermic patients
As the largest proportion of male infertility population, asthenozoospermia patients often resort to sperm cryopreservation to preserve fertility as well as to enrich motile sperm for assisted reproductive techniques (ART), although it may cause some cryodamage during the freezing–thawing process. T...
Gespeichert in:
| Veröffentlicht in: | Cryobiology 2019-12, Vol.91, p.18-22 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 22 |
|---|---|
| container_issue | |
| container_start_page | 18 |
| container_title | Cryobiology |
| container_volume | 91 |
| creator | Zhang, Xuelian Lu, Xilan Li, Juntao Xia, Qing Gao, Jiangang Wu, Bin |
| description | As the largest proportion of male infertility population, asthenozoospermia patients often resort to sperm cryopreservation to preserve fertility as well as to enrich motile sperm for assisted reproductive techniques (ART), although it may cause some cryodamage during the freezing–thawing process. The objective of this study was to investigate whether mitochondrial antioxidant Mito-Tempo was effective in preventing cryodamage of asthenozoospermic spermatozoa. Asthenozoospermic semen samples were collected and cryopreserved in media supplemented with different concentrations (0.0, 1.0, 10 and 100 μM) of Mito-Tempo. We measured sperm motility, viability, membrane integrity, DNA fragmentation, mitochondrial membrane potential, oxidation product, and antioxidant enzymes activities. Supplementation of the cryopreservation media with Mito-Tempo (10 and 100 μM) induced a significant improvement in sperm viability, motility, membrane integrity, mitochondrial membrane potential and chromatin integrity (P |
| doi_str_mv | 10.1016/j.cryobiol.2019.11.005 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2315529036</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0011224019302135</els_id><sourcerecordid>2315529036</sourcerecordid><originalsourceid>FETCH-LOGICAL-c368t-467447687c09ca51da4394b13bc327e27ec9e143f66a0d023095282f679d9fa03</originalsourceid><addsrcrecordid>eNqFkE2P1DAMhiMEYoeFv7DKkUuLnbTp5AZa8SUt4rKcozR1h4zapiSZEcOJn06G2eWKZMmy_b62_DB2g1AjoHqzr108hd6HqRaAukasAdonbIOgoRJSi6dsA4BYCdHAFXuR0h4AVCeb5-xKYskoug37_cXnUN3TvAZup4mO3mZK_Lx8sLPdEe9PPNLuMNnslx33S47W0TSVRuThpx9K_0h8pmz7MPk0FwlPK8XZ5vArWD7GMHOb8ndaSh3-jrzja_HRktNL9my0U6JXD_maffvw_v72U3X39ePn23d3lZNqm6tGdU3TqW3nQDvb4mAbqZseZe-k6KiE04SNHJWyMICQoFuxFaPq9KBHC_Kavb7sXWP4caCUzezT-RG7UDgkIyS2rdAgVZGqi9TFkFKk0azRzzaeDII50zd780jfnOkbRFPoF-PNw41DP9Pwz_aIuwjeXgRUPj16iia5QsHR4CO5bIbg_3fjD9FXnPk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2315529036</pqid></control><display><type>article</type><title>Mito-Tempo alleviates cryodamage by regulating intracellular oxidative metabolism in spermatozoa from asthenozoospermic patients</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Zhang, Xuelian ; Lu, Xilan ; Li, Juntao ; Xia, Qing ; Gao, Jiangang ; Wu, Bin</creator><creatorcontrib>Zhang, Xuelian ; Lu, Xilan ; Li, Juntao ; Xia, Qing ; Gao, Jiangang ; Wu, Bin</creatorcontrib><description>As the largest proportion of male infertility population, asthenozoospermia patients often resort to sperm cryopreservation to preserve fertility as well as to enrich motile sperm for assisted reproductive techniques (ART), although it may cause some cryodamage during the freezing–thawing process. The objective of this study was to investigate whether mitochondrial antioxidant Mito-Tempo was effective in preventing cryodamage of asthenozoospermic spermatozoa. Asthenozoospermic semen samples were collected and cryopreserved in media supplemented with different concentrations (0.0, 1.0, 10 and 100 μM) of Mito-Tempo. We measured sperm motility, viability, membrane integrity, DNA fragmentation, mitochondrial membrane potential, oxidation product, and antioxidant enzymes activities. Supplementation of the cryopreservation media with Mito-Tempo (10 and 100 μM) induced a significant improvement in sperm viability, motility, membrane integrity, mitochondrial membrane potential and chromatin integrity (P < 0.05). Significant enhancement of antioxidant enzymes activities accompanied by the decreased formation of oxidation products (ROS and MDA) was also observed in groups supplemented with Mito-Tempo (10 and 100 μM). It is concluded that mitochondria targeted antioxidant Mito-Tempo alleviates cryodamage by regulating intracellular oxidative metabolism in spermatozoa from asthenozoospermic patients after cryopreservation.</description><identifier>ISSN: 0011-2240</identifier><identifier>EISSN: 1090-2392</identifier><identifier>DOI: 10.1016/j.cryobiol.2019.11.005</identifier><identifier>PMID: 31734127</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Antioxidants - pharmacology ; Asthenozoospermia ; Asthenozoospermia - metabolism ; Chromatin ; Cryopreservation ; Cryopreservation - methods ; Cryoprotective Agents - pharmacology ; DNA Fragmentation - drug effects ; Fertility - drug effects ; Freezing ; Humans ; Male ; Membrane Potential, Mitochondrial - drug effects ; Mito-tempo ; Mitochondria - metabolism ; Oxidative Stress - drug effects ; Semen Analysis - methods ; Semen Preservation - methods ; Sperm ; Sperm Motility - drug effects ; Spermatozoa - drug effects</subject><ispartof>Cryobiology, 2019-12, Vol.91, p.18-22</ispartof><rights>2019</rights><rights>Copyright © 2019. Published by Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-467447687c09ca51da4394b13bc327e27ec9e143f66a0d023095282f679d9fa03</citedby><cites>FETCH-LOGICAL-c368t-467447687c09ca51da4394b13bc327e27ec9e143f66a0d023095282f679d9fa03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cryobiol.2019.11.005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31734127$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Xuelian</creatorcontrib><creatorcontrib>Lu, Xilan</creatorcontrib><creatorcontrib>Li, Juntao</creatorcontrib><creatorcontrib>Xia, Qing</creatorcontrib><creatorcontrib>Gao, Jiangang</creatorcontrib><creatorcontrib>Wu, Bin</creatorcontrib><title>Mito-Tempo alleviates cryodamage by regulating intracellular oxidative metabolism in spermatozoa from asthenozoospermic patients</title><title>Cryobiology</title><addtitle>Cryobiology</addtitle><description>As the largest proportion of male infertility population, asthenozoospermia patients often resort to sperm cryopreservation to preserve fertility as well as to enrich motile sperm for assisted reproductive techniques (ART), although it may cause some cryodamage during the freezing–thawing process. The objective of this study was to investigate whether mitochondrial antioxidant Mito-Tempo was effective in preventing cryodamage of asthenozoospermic spermatozoa. Asthenozoospermic semen samples were collected and cryopreserved in media supplemented with different concentrations (0.0, 1.0, 10 and 100 μM) of Mito-Tempo. We measured sperm motility, viability, membrane integrity, DNA fragmentation, mitochondrial membrane potential, oxidation product, and antioxidant enzymes activities. Supplementation of the cryopreservation media with Mito-Tempo (10 and 100 μM) induced a significant improvement in sperm viability, motility, membrane integrity, mitochondrial membrane potential and chromatin integrity (P < 0.05). Significant enhancement of antioxidant enzymes activities accompanied by the decreased formation of oxidation products (ROS and MDA) was also observed in groups supplemented with Mito-Tempo (10 and 100 μM). It is concluded that mitochondria targeted antioxidant Mito-Tempo alleviates cryodamage by regulating intracellular oxidative metabolism in spermatozoa from asthenozoospermic patients after cryopreservation.</description><subject>Antioxidants - pharmacology</subject><subject>Asthenozoospermia</subject><subject>Asthenozoospermia - metabolism</subject><subject>Chromatin</subject><subject>Cryopreservation</subject><subject>Cryopreservation - methods</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>DNA Fragmentation - drug effects</subject><subject>Fertility - drug effects</subject><subject>Freezing</subject><subject>Humans</subject><subject>Male</subject><subject>Membrane Potential, Mitochondrial - drug effects</subject><subject>Mito-tempo</subject><subject>Mitochondria - metabolism</subject><subject>Oxidative Stress - drug effects</subject><subject>Semen Analysis - methods</subject><subject>Semen Preservation - methods</subject><subject>Sperm</subject><subject>Sperm Motility - drug effects</subject><subject>Spermatozoa - drug effects</subject><issn>0011-2240</issn><issn>1090-2392</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2P1DAMhiMEYoeFv7DKkUuLnbTp5AZa8SUt4rKcozR1h4zapiSZEcOJn06G2eWKZMmy_b62_DB2g1AjoHqzr108hd6HqRaAukasAdonbIOgoRJSi6dsA4BYCdHAFXuR0h4AVCeb5-xKYskoug37_cXnUN3TvAZup4mO3mZK_Lx8sLPdEe9PPNLuMNnslx33S47W0TSVRuThpx9K_0h8pmz7MPk0FwlPK8XZ5vArWD7GMHOb8ndaSh3-jrzja_HRktNL9my0U6JXD_maffvw_v72U3X39ePn23d3lZNqm6tGdU3TqW3nQDvb4mAbqZseZe-k6KiE04SNHJWyMICQoFuxFaPq9KBHC_Kavb7sXWP4caCUzezT-RG7UDgkIyS2rdAgVZGqi9TFkFKk0azRzzaeDII50zd780jfnOkbRFPoF-PNw41DP9Pwz_aIuwjeXgRUPj16iia5QsHR4CO5bIbg_3fjD9FXnPk</recordid><startdate>201912</startdate><enddate>201912</enddate><creator>Zhang, Xuelian</creator><creator>Lu, Xilan</creator><creator>Li, Juntao</creator><creator>Xia, Qing</creator><creator>Gao, Jiangang</creator><creator>Wu, Bin</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201912</creationdate><title>Mito-Tempo alleviates cryodamage by regulating intracellular oxidative metabolism in spermatozoa from asthenozoospermic patients</title><author>Zhang, Xuelian ; Lu, Xilan ; Li, Juntao ; Xia, Qing ; Gao, Jiangang ; Wu, Bin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-467447687c09ca51da4394b13bc327e27ec9e143f66a0d023095282f679d9fa03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antioxidants - pharmacology</topic><topic>Asthenozoospermia</topic><topic>Asthenozoospermia - metabolism</topic><topic>Chromatin</topic><topic>Cryopreservation</topic><topic>Cryopreservation - methods</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>DNA Fragmentation - drug effects</topic><topic>Fertility - drug effects</topic><topic>Freezing</topic><topic>Humans</topic><topic>Male</topic><topic>Membrane Potential, Mitochondrial - drug effects</topic><topic>Mito-tempo</topic><topic>Mitochondria - metabolism</topic><topic>Oxidative Stress - drug effects</topic><topic>Semen Analysis - methods</topic><topic>Semen Preservation - methods</topic><topic>Sperm</topic><topic>Sperm Motility - drug effects</topic><topic>Spermatozoa - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Xuelian</creatorcontrib><creatorcontrib>Lu, Xilan</creatorcontrib><creatorcontrib>Li, Juntao</creatorcontrib><creatorcontrib>Xia, Qing</creatorcontrib><creatorcontrib>Gao, Jiangang</creatorcontrib><creatorcontrib>Wu, Bin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cryobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Xuelian</au><au>Lu, Xilan</au><au>Li, Juntao</au><au>Xia, Qing</au><au>Gao, Jiangang</au><au>Wu, Bin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mito-Tempo alleviates cryodamage by regulating intracellular oxidative metabolism in spermatozoa from asthenozoospermic patients</atitle><jtitle>Cryobiology</jtitle><addtitle>Cryobiology</addtitle><date>2019-12</date><risdate>2019</risdate><volume>91</volume><spage>18</spage><epage>22</epage><pages>18-22</pages><issn>0011-2240</issn><eissn>1090-2392</eissn><abstract>As the largest proportion of male infertility population, asthenozoospermia patients often resort to sperm cryopreservation to preserve fertility as well as to enrich motile sperm for assisted reproductive techniques (ART), although it may cause some cryodamage during the freezing–thawing process. The objective of this study was to investigate whether mitochondrial antioxidant Mito-Tempo was effective in preventing cryodamage of asthenozoospermic spermatozoa. Asthenozoospermic semen samples were collected and cryopreserved in media supplemented with different concentrations (0.0, 1.0, 10 and 100 μM) of Mito-Tempo. We measured sperm motility, viability, membrane integrity, DNA fragmentation, mitochondrial membrane potential, oxidation product, and antioxidant enzymes activities. Supplementation of the cryopreservation media with Mito-Tempo (10 and 100 μM) induced a significant improvement in sperm viability, motility, membrane integrity, mitochondrial membrane potential and chromatin integrity (P < 0.05). Significant enhancement of antioxidant enzymes activities accompanied by the decreased formation of oxidation products (ROS and MDA) was also observed in groups supplemented with Mito-Tempo (10 and 100 μM). It is concluded that mitochondria targeted antioxidant Mito-Tempo alleviates cryodamage by regulating intracellular oxidative metabolism in spermatozoa from asthenozoospermic patients after cryopreservation.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>31734127</pmid><doi>10.1016/j.cryobiol.2019.11.005</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0011-2240 |
| ispartof | Cryobiology, 2019-12, Vol.91, p.18-22 |
| issn | 0011-2240 1090-2392 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2315529036 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Antioxidants - pharmacology Asthenozoospermia Asthenozoospermia - metabolism Chromatin Cryopreservation Cryopreservation - methods Cryoprotective Agents - pharmacology DNA Fragmentation - drug effects Fertility - drug effects Freezing Humans Male Membrane Potential, Mitochondrial - drug effects Mito-tempo Mitochondria - metabolism Oxidative Stress - drug effects Semen Analysis - methods Semen Preservation - methods Sperm Sperm Motility - drug effects Spermatozoa - drug effects |
| title | Mito-Tempo alleviates cryodamage by regulating intracellular oxidative metabolism in spermatozoa from asthenozoospermic patients |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T21%3A11%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Mito-Tempo%20alleviates%20cryodamage%20by%20regulating%20intracellular%20oxidative%20metabolism%20in%20spermatozoa%20from%20asthenozoospermic%20patients&rft.jtitle=Cryobiology&rft.au=Zhang,%20Xuelian&rft.date=2019-12&rft.volume=91&rft.spage=18&rft.epage=22&rft.pages=18-22&rft.issn=0011-2240&rft.eissn=1090-2392&rft_id=info:doi/10.1016/j.cryobiol.2019.11.005&rft_dat=%3Cproquest_cross%3E2315529036%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2315529036&rft_id=info:pmid/31734127&rft_els_id=S0011224019302135&rfr_iscdi=true |