A novel experimental immunomodulatory therapy against Nocardia brasiliensis in a BALB/c murine model
Summary Background Mycetoma is recognized as a neglected tropical disease and there are still therapeutic challenges, especially in cases recalcitrant to standard therapy or with high risk of dissemination. Subcultures have been used previously to decrease the virulence of human pathogens. Previous...
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| Veröffentlicht in: | Clinical and experimental dermatology 2020-07, Vol.45 (5), p.544-548 |
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| creator | García‐Lozano, J. A. García‐Berlanga, C. C. Viveros‐Rosado, R. T. Ocampo‐Candiani, J. Vargas‐Villarreal, J. Vera‐Cabrera, L. |
| description | Summary
Background
Mycetoma is recognized as a neglected tropical disease and there are still therapeutic challenges, especially in cases recalcitrant to standard therapy or with high risk of dissemination. Subcultures have been used previously to decrease the virulence of human pathogens. Previous reports have demonstrated that after carrying out 200 subcultures of Nocardia brasiliensis, a decrease in virulence was observed.
Aim
To evaluate the effect of attenuated N. brasiliensis strains on the development of lesions in an established mycetoma infection.
Methods
Female 8–12‐week‐old BALB/c mice were injected with N. brasiliensis suspension to establish a mycetoma. Sixty mice were selected and divided into three groups: two of these groups were inoculated in the dorsum with N. brasiliensis subcultured 200 and 400 times, respectively, while the third group served as control. The thickness of each lesion was measured with calipers every week for 12 weeks.
Results
After 12 weeks, we observed that inoculation of 1 × 105 colony‐forming units of attenuated N. brasiliensis strains was able to modify the natural history of the infection, with a decrease in the size of the lesions, particularly with P400, compared with the control group (P |
| doi_str_mv | 10.1111/ced.14139 |
| format | Article |
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Background
Mycetoma is recognized as a neglected tropical disease and there are still therapeutic challenges, especially in cases recalcitrant to standard therapy or with high risk of dissemination. Subcultures have been used previously to decrease the virulence of human pathogens. Previous reports have demonstrated that after carrying out 200 subcultures of Nocardia brasiliensis, a decrease in virulence was observed.
Aim
To evaluate the effect of attenuated N. brasiliensis strains on the development of lesions in an established mycetoma infection.
Methods
Female 8–12‐week‐old BALB/c mice were injected with N. brasiliensis suspension to establish a mycetoma. Sixty mice were selected and divided into three groups: two of these groups were inoculated in the dorsum with N. brasiliensis subcultured 200 and 400 times, respectively, while the third group served as control. The thickness of each lesion was measured with calipers every week for 12 weeks.
Results
After 12 weeks, we observed that inoculation of 1 × 105 colony‐forming units of attenuated N. brasiliensis strains was able to modify the natural history of the infection, with a decrease in the size of the lesions, particularly with P400, compared with the control group (P < 0.01).
Conclusion
In this experimental evaluation of an immunomodulatory therapy with attenuated N. brasiliensis strains in a murine model, there was a greater stability in the size of the lesion over time in BALB/c mice inoculated with the P400 strain. This treatment could open the possibility of using the attenuated strain as immunomodulatory therapy in patients recalcitrant to standard therapy, with high risk of dissemination or who develop drug‐related adverse effects.</description><identifier>ISSN: 0307-6938</identifier><identifier>EISSN: 1365-2230</identifier><identifier>DOI: 10.1111/ced.14139</identifier><identifier>PMID: 31729068</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Animal models ; Immunomodulation ; Immunomodulators ; Inoculation ; Lesions ; Nocardia ; Virulence</subject><ispartof>Clinical and experimental dermatology, 2020-07, Vol.45 (5), p.544-548</ispartof><rights>2019 British Association of Dermatologists</rights><rights>2019 British Association of Dermatologists.</rights><rights>Copyright © 2020 British Association of Dermatologists</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3139-d6e0b0ed7d6547a4c4bc56e1fe9ef4c234ce79cb2dbd3e5d27617f59dd53641d3</cites><orcidid>0000-0002-0213-0031 ; 0000-0002-6958-7511 ; 0000-0002-0890-9660</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31729068$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>García‐Lozano, J. A.</creatorcontrib><creatorcontrib>García‐Berlanga, C. C.</creatorcontrib><creatorcontrib>Viveros‐Rosado, R. T.</creatorcontrib><creatorcontrib>Ocampo‐Candiani, J.</creatorcontrib><creatorcontrib>Vargas‐Villarreal, J.</creatorcontrib><creatorcontrib>Vera‐Cabrera, L.</creatorcontrib><title>A novel experimental immunomodulatory therapy against Nocardia brasiliensis in a BALB/c murine model</title><title>Clinical and experimental dermatology</title><addtitle>Clin Exp Dermatol</addtitle><description>Summary
Background
Mycetoma is recognized as a neglected tropical disease and there are still therapeutic challenges, especially in cases recalcitrant to standard therapy or with high risk of dissemination. Subcultures have been used previously to decrease the virulence of human pathogens. Previous reports have demonstrated that after carrying out 200 subcultures of Nocardia brasiliensis, a decrease in virulence was observed.
Aim
To evaluate the effect of attenuated N. brasiliensis strains on the development of lesions in an established mycetoma infection.
Methods
Female 8–12‐week‐old BALB/c mice were injected with N. brasiliensis suspension to establish a mycetoma. Sixty mice were selected and divided into three groups: two of these groups were inoculated in the dorsum with N. brasiliensis subcultured 200 and 400 times, respectively, while the third group served as control. The thickness of each lesion was measured with calipers every week for 12 weeks.
Results
After 12 weeks, we observed that inoculation of 1 × 105 colony‐forming units of attenuated N. brasiliensis strains was able to modify the natural history of the infection, with a decrease in the size of the lesions, particularly with P400, compared with the control group (P < 0.01).
Conclusion
In this experimental evaluation of an immunomodulatory therapy with attenuated N. brasiliensis strains in a murine model, there was a greater stability in the size of the lesion over time in BALB/c mice inoculated with the P400 strain. This treatment could open the possibility of using the attenuated strain as immunomodulatory therapy in patients recalcitrant to standard therapy, with high risk of dissemination or who develop drug‐related adverse effects.</description><subject>Animal models</subject><subject>Immunomodulation</subject><subject>Immunomodulators</subject><subject>Inoculation</subject><subject>Lesions</subject><subject>Nocardia</subject><subject>Virulence</subject><issn>0307-6938</issn><issn>1365-2230</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp1kE1PGzEQQK2KqoS0h_4BZIkLHJb4Y9cbH0OaQqUILu3Z8tqzxchrB3u3kH9ft4ELEnMZafT0NHoIfaXkkpZZGLCXtKZcfkAzykVTMcbJEZoRTtpKSL48Ric5PxBCOW2bT-i4LCaJWM6QXeEQ_4DH8LyD5AYIo_bYDcMU4hDt5PUY0x6P95D0bo_1b-1CHvFtNDpZp3GXdHbeQcguYxewxler7dXC4GFKLgAuDvCf0cde-wxfXvYc_fq--bm-qbZ31z_Wq21leHm-sgJIR8C2VjR1q2tTd6YRQHuQ0NeG8dpAK03HbGc5NJa1grZ9I61tuKip5XN0fvDuUnycII9qcNmA9zpAnLJinDZEckJFQc_eoA9xSqF8p1hNJJVLIlihLg6USTHnBL3alUY67RUl6l96VdKr_-kLe_pinLqhXF_J19YFWByAJ-dh_75JrTffDsq_JOSNpQ</recordid><startdate>202007</startdate><enddate>202007</enddate><creator>García‐Lozano, J. A.</creator><creator>García‐Berlanga, C. C.</creator><creator>Viveros‐Rosado, R. T.</creator><creator>Ocampo‐Candiani, J.</creator><creator>Vargas‐Villarreal, J.</creator><creator>Vera‐Cabrera, L.</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0213-0031</orcidid><orcidid>https://orcid.org/0000-0002-6958-7511</orcidid><orcidid>https://orcid.org/0000-0002-0890-9660</orcidid></search><sort><creationdate>202007</creationdate><title>A novel experimental immunomodulatory therapy against Nocardia brasiliensis in a BALB/c murine model</title><author>García‐Lozano, J. A. ; García‐Berlanga, C. C. ; Viveros‐Rosado, R. T. ; Ocampo‐Candiani, J. ; Vargas‐Villarreal, J. ; Vera‐Cabrera, L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3139-d6e0b0ed7d6547a4c4bc56e1fe9ef4c234ce79cb2dbd3e5d27617f59dd53641d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animal models</topic><topic>Immunomodulation</topic><topic>Immunomodulators</topic><topic>Inoculation</topic><topic>Lesions</topic><topic>Nocardia</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>García‐Lozano, J. A.</creatorcontrib><creatorcontrib>García‐Berlanga, C. C.</creatorcontrib><creatorcontrib>Viveros‐Rosado, R. T.</creatorcontrib><creatorcontrib>Ocampo‐Candiani, J.</creatorcontrib><creatorcontrib>Vargas‐Villarreal, J.</creatorcontrib><creatorcontrib>Vera‐Cabrera, L.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical and experimental dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>García‐Lozano, J. A.</au><au>García‐Berlanga, C. C.</au><au>Viveros‐Rosado, R. T.</au><au>Ocampo‐Candiani, J.</au><au>Vargas‐Villarreal, J.</au><au>Vera‐Cabrera, L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel experimental immunomodulatory therapy against Nocardia brasiliensis in a BALB/c murine model</atitle><jtitle>Clinical and experimental dermatology</jtitle><addtitle>Clin Exp Dermatol</addtitle><date>2020-07</date><risdate>2020</risdate><volume>45</volume><issue>5</issue><spage>544</spage><epage>548</epage><pages>544-548</pages><issn>0307-6938</issn><eissn>1365-2230</eissn><abstract>Summary
Background
Mycetoma is recognized as a neglected tropical disease and there are still therapeutic challenges, especially in cases recalcitrant to standard therapy or with high risk of dissemination. Subcultures have been used previously to decrease the virulence of human pathogens. Previous reports have demonstrated that after carrying out 200 subcultures of Nocardia brasiliensis, a decrease in virulence was observed.
Aim
To evaluate the effect of attenuated N. brasiliensis strains on the development of lesions in an established mycetoma infection.
Methods
Female 8–12‐week‐old BALB/c mice were injected with N. brasiliensis suspension to establish a mycetoma. Sixty mice were selected and divided into three groups: two of these groups were inoculated in the dorsum with N. brasiliensis subcultured 200 and 400 times, respectively, while the third group served as control. The thickness of each lesion was measured with calipers every week for 12 weeks.
Results
After 12 weeks, we observed that inoculation of 1 × 105 colony‐forming units of attenuated N. brasiliensis strains was able to modify the natural history of the infection, with a decrease in the size of the lesions, particularly with P400, compared with the control group (P < 0.01).
Conclusion
In this experimental evaluation of an immunomodulatory therapy with attenuated N. brasiliensis strains in a murine model, there was a greater stability in the size of the lesion over time in BALB/c mice inoculated with the P400 strain. This treatment could open the possibility of using the attenuated strain as immunomodulatory therapy in patients recalcitrant to standard therapy, with high risk of dissemination or who develop drug‐related adverse effects.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>31729068</pmid><doi>10.1111/ced.14139</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-0213-0031</orcidid><orcidid>https://orcid.org/0000-0002-6958-7511</orcidid><orcidid>https://orcid.org/0000-0002-0890-9660</orcidid></addata></record> |
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| ispartof | Clinical and experimental dermatology, 2020-07, Vol.45 (5), p.544-548 |
| issn | 0307-6938 1365-2230 |
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| source | Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Animal models Immunomodulation Immunomodulators Inoculation Lesions Nocardia Virulence |
| title | A novel experimental immunomodulatory therapy against Nocardia brasiliensis in a BALB/c murine model |
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