Validation of the AGCU Expressmarker 16 + 22Y Kit: a new multiplex for forensic application
Genotyping by targeting short tandem repeats (STRs) has been widely used in forensic applications. However, most commercial kits detect autosomal STRs or Y-STRs alone, which waste both time and opportunity. The AGCU Expressmarker 16 + 22Y Kit includes 16 autosomal and 22 Y-chromosomal STR loci and i...
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| Veröffentlicht in: | International journal of legal medicine 2020, Vol.134 (1), p.177-183 |
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| creator | Xie, Mingkun Song, Feng Li, Jienan Xie, Bowen Wang, Shuangshuang Wang, Weizhi Ma, Hong Luo, Haibo |
| description | Genotyping by targeting short tandem repeats (STRs) has been widely used in forensic applications. However, most commercial kits detect autosomal STRs or Y-STRs alone, which waste both time and opportunity. The AGCU Expressmarker 16 + 22Y Kit includes 16 autosomal and 22 Y-chromosomal STR loci and is designed for the forensic science field and obtaining quicker results. Here, we conducted the validation study according to Scientific Working Group on DNA Analysis Methods (SWGDAM) guidelines. Validation of PCR-based studies, species specificity, sensitivity, DNA mixture studies, inhibitors, precision, and sizing accuracy were performed. Furthermore, this system was also tested in 346 random male samples from Han, Hui, Tibetan, and Zhuang populations in China, showing its high power for forensic discrimination in the Chinese population. In addition, this system was able to deal with AMELY deletion cases, which can correctly identify sex in forensic criminal investigations. Our results suggested that the AGCU Expressmarker 16 + 22Y Kit is a useful tool for rapid criminal investigation. |
| doi_str_mv | 10.1007/s00414-019-02200-3 |
| format | Article |
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However, most commercial kits detect autosomal STRs or Y-STRs alone, which waste both time and opportunity. The AGCU Expressmarker 16 + 22Y Kit includes 16 autosomal and 22 Y-chromosomal STR loci and is designed for the forensic science field and obtaining quicker results. Here, we conducted the validation study according to Scientific Working Group on DNA Analysis Methods (SWGDAM) guidelines. Validation of PCR-based studies, species specificity, sensitivity, DNA mixture studies, inhibitors, precision, and sizing accuracy were performed. Furthermore, this system was also tested in 346 random male samples from Han, Hui, Tibetan, and Zhuang populations in China, showing its high power for forensic discrimination in the Chinese population. In addition, this system was able to deal with AMELY deletion cases, which can correctly identify sex in forensic criminal investigations. Our results suggested that the AGCU Expressmarker 16 + 22Y Kit is a useful tool for rapid criminal investigation.</description><identifier>ISSN: 0937-9827</identifier><identifier>EISSN: 1437-1596</identifier><identifier>DOI: 10.1007/s00414-019-02200-3</identifier><identifier>PMID: 31713677</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Blood ; China - ethnology ; Crime ; DNA Fingerprinting - instrumentation ; Ethnic Groups - genetics ; Female ; Forensic Medicine ; Forensic science ; Forensic sciences ; Forensic Sciences - instrumentation ; Genetic Loci ; Genotype ; Genotype & phenotype ; Humans ; Male ; Medical Law ; Medicine ; Medicine & Public Health ; Microsatellite Repeats ; Polymerase Chain Reaction - methods ; Population genetics ; Saliva ; Sensitivity analysis ; Sensitivity and Specificity ; Sequence Analysis, DNA - methods ; Short Communication ; Species Specificity ; Y Chromosome ; Y chromosomes</subject><ispartof>International journal of legal medicine, 2020, Vol.134 (1), p.177-183</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2019</rights><rights>International Journal of Legal Medicine is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-60b9e86ffe0f4f2223c713ba0b36345343d9057f5aeb2c8e1f5248d48a57d0d33</citedby><cites>FETCH-LOGICAL-c375t-60b9e86ffe0f4f2223c713ba0b36345343d9057f5aeb2c8e1f5248d48a57d0d33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00414-019-02200-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00414-019-02200-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31713677$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xie, Mingkun</creatorcontrib><creatorcontrib>Song, Feng</creatorcontrib><creatorcontrib>Li, Jienan</creatorcontrib><creatorcontrib>Xie, Bowen</creatorcontrib><creatorcontrib>Wang, Shuangshuang</creatorcontrib><creatorcontrib>Wang, Weizhi</creatorcontrib><creatorcontrib>Ma, Hong</creatorcontrib><creatorcontrib>Luo, Haibo</creatorcontrib><title>Validation of the AGCU Expressmarker 16 + 22Y Kit: a new multiplex for forensic application</title><title>International journal of legal medicine</title><addtitle>Int J Legal Med</addtitle><addtitle>Int J Legal Med</addtitle><description>Genotyping by targeting short tandem repeats (STRs) has been widely used in forensic applications. However, most commercial kits detect autosomal STRs or Y-STRs alone, which waste both time and opportunity. The AGCU Expressmarker 16 + 22Y Kit includes 16 autosomal and 22 Y-chromosomal STR loci and is designed for the forensic science field and obtaining quicker results. Here, we conducted the validation study according to Scientific Working Group on DNA Analysis Methods (SWGDAM) guidelines. Validation of PCR-based studies, species specificity, sensitivity, DNA mixture studies, inhibitors, precision, and sizing accuracy were performed. Furthermore, this system was also tested in 346 random male samples from Han, Hui, Tibetan, and Zhuang populations in China, showing its high power for forensic discrimination in the Chinese population. In addition, this system was able to deal with AMELY deletion cases, which can correctly identify sex in forensic criminal investigations. Our results suggested that the AGCU Expressmarker 16 + 22Y Kit is a useful tool for rapid criminal investigation.</description><subject>Animals</subject><subject>Blood</subject><subject>China - ethnology</subject><subject>Crime</subject><subject>DNA Fingerprinting - instrumentation</subject><subject>Ethnic Groups - genetics</subject><subject>Female</subject><subject>Forensic Medicine</subject><subject>Forensic science</subject><subject>Forensic sciences</subject><subject>Forensic Sciences - instrumentation</subject><subject>Genetic Loci</subject><subject>Genotype</subject><subject>Genotype & phenotype</subject><subject>Humans</subject><subject>Male</subject><subject>Medical Law</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Microsatellite Repeats</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Population genetics</subject><subject>Saliva</subject><subject>Sensitivity analysis</subject><subject>Sensitivity and Specificity</subject><subject>Sequence Analysis, DNA - methods</subject><subject>Short Communication</subject><subject>Species Specificity</subject><subject>Y Chromosome</subject><subject>Y chromosomes</subject><issn>0937-9827</issn><issn>1437-1596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kF1rFDEUhoNY3O3qH_BCAt4IZerJ12TGu7K0VbrQG1cQL0Jm5kRnnS-TGbr992Z3VoVeeBFOIM958_IQ8prBJQPQ7wOAZDIBlifAOUAinpElk0InTOXpc7KEPN7zjOsFOQ9hB8B0qtULshBMM5FqvSTfvtimruxY9x3tHR1_IL26XW_p9X7wGEJr_U_0lKX0gnL-ld7V4wdqaYcPtJ2asR4a3FPX-8PBLtQltcPQ1OUx8CU5c7YJ-Oo0V2R7c_15_THZ3N9-Wl9tklJoNSYpFDlmqXMITjrOuShju8JCIVIhlZCiykFppywWvMyQOcVlVsnMKl1BJcSKvJtzB9__mjCMpq1DiU1jO-ynYLhgEiDTkEX07RN010--i-0iJQXPlVA6UnymSt-H4NGZwddRxaNhYA7qzazeRPXmqN4cWrw5RU9Fi9XflT-uIyBmIMSn7jv6f3__J_Y3uWCLww</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>Xie, Mingkun</creator><creator>Song, Feng</creator><creator>Li, Jienan</creator><creator>Xie, Bowen</creator><creator>Wang, Shuangshuang</creator><creator>Wang, Weizhi</creator><creator>Ma, Hong</creator><creator>Luo, Haibo</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>0-V</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AM</scope><scope>8AO</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ALSLI</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BGRYB</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>HCIFZ</scope><scope>K7.</scope><scope>K9.</scope><scope>L6V</scope><scope>M0O</scope><scope>M0S</scope><scope>M1P</scope><scope>M7S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>2020</creationdate><title>Validation of the AGCU Expressmarker 16 + 22Y Kit: a new multiplex for forensic application</title><author>Xie, Mingkun ; Song, Feng ; Li, Jienan ; Xie, Bowen ; Wang, Shuangshuang ; Wang, Weizhi ; Ma, Hong ; Luo, Haibo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-60b9e86ffe0f4f2223c713ba0b36345343d9057f5aeb2c8e1f5248d48a57d0d33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Blood</topic><topic>China - ethnology</topic><topic>Crime</topic><topic>DNA Fingerprinting - instrumentation</topic><topic>Ethnic Groups - genetics</topic><topic>Female</topic><topic>Forensic Medicine</topic><topic>Forensic science</topic><topic>Forensic sciences</topic><topic>Forensic Sciences - instrumentation</topic><topic>Genetic Loci</topic><topic>Genotype</topic><topic>Genotype & phenotype</topic><topic>Humans</topic><topic>Male</topic><topic>Medical Law</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Microsatellite Repeats</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Population genetics</topic><topic>Saliva</topic><topic>Sensitivity analysis</topic><topic>Sensitivity and Specificity</topic><topic>Sequence Analysis, DNA - methods</topic><topic>Short Communication</topic><topic>Species Specificity</topic><topic>Y Chromosome</topic><topic>Y chromosomes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xie, Mingkun</creatorcontrib><creatorcontrib>Song, Feng</creatorcontrib><creatorcontrib>Li, Jienan</creatorcontrib><creatorcontrib>Xie, Bowen</creatorcontrib><creatorcontrib>Wang, Shuangshuang</creatorcontrib><creatorcontrib>Wang, Weizhi</creatorcontrib><creatorcontrib>Ma, Hong</creatorcontrib><creatorcontrib>Luo, Haibo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Social Sciences Premium Collection【Remote access available】</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Criminal Justice Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Social Science Premium Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Criminology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Criminal Justice (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Criminal Justice</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Engineering Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of legal medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xie, Mingkun</au><au>Song, Feng</au><au>Li, Jienan</au><au>Xie, Bowen</au><au>Wang, Shuangshuang</au><au>Wang, Weizhi</au><au>Ma, Hong</au><au>Luo, Haibo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Validation of the AGCU Expressmarker 16 + 22Y Kit: a new multiplex for forensic application</atitle><jtitle>International journal of legal medicine</jtitle><stitle>Int J Legal Med</stitle><addtitle>Int J Legal Med</addtitle><date>2020</date><risdate>2020</risdate><volume>134</volume><issue>1</issue><spage>177</spage><epage>183</epage><pages>177-183</pages><issn>0937-9827</issn><eissn>1437-1596</eissn><abstract>Genotyping by targeting short tandem repeats (STRs) has been widely used in forensic applications. However, most commercial kits detect autosomal STRs or Y-STRs alone, which waste both time and opportunity. The AGCU Expressmarker 16 + 22Y Kit includes 16 autosomal and 22 Y-chromosomal STR loci and is designed for the forensic science field and obtaining quicker results. Here, we conducted the validation study according to Scientific Working Group on DNA Analysis Methods (SWGDAM) guidelines. Validation of PCR-based studies, species specificity, sensitivity, DNA mixture studies, inhibitors, precision, and sizing accuracy were performed. Furthermore, this system was also tested in 346 random male samples from Han, Hui, Tibetan, and Zhuang populations in China, showing its high power for forensic discrimination in the Chinese population. In addition, this system was able to deal with AMELY deletion cases, which can correctly identify sex in forensic criminal investigations. Our results suggested that the AGCU Expressmarker 16 + 22Y Kit is a useful tool for rapid criminal investigation.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>31713677</pmid><doi>10.1007/s00414-019-02200-3</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; HeinOnline Law Journal Library; SpringerLink Journals - AutoHoldings |
| subjects | Animals Blood China - ethnology Crime DNA Fingerprinting - instrumentation Ethnic Groups - genetics Female Forensic Medicine Forensic science Forensic sciences Forensic Sciences - instrumentation Genetic Loci Genotype Genotype & phenotype Humans Male Medical Law Medicine Medicine & Public Health Microsatellite Repeats Polymerase Chain Reaction - methods Population genetics Saliva Sensitivity analysis Sensitivity and Specificity Sequence Analysis, DNA - methods Short Communication Species Specificity Y Chromosome Y chromosomes |
| title | Validation of the AGCU Expressmarker 16 + 22Y Kit: a new multiplex for forensic application |
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