Effect of miR-181a-3p on osteogenic differentiation of human bone marrow-derived mesenchymal stem cells by targeting BMP10
To explore the regulation relationship between miR-181a-3p and BMP10, and their mechanism of osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (MSCs). After osteogenic induction of MSCs, the ALP activity was detected by ELISA. The expression of miRNA-181a-3p and BMP10 wa...
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| Veröffentlicht in: | Artificial cells, nanomedicine, and biotechnology nanomedicine, and biotechnology, 2019-12, Vol.47 (1), p.4159-4164 |
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| container_title | Artificial cells, nanomedicine, and biotechnology |
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| creator | Tao, GuiLu Mao, Ping Guan, HaoNan Jiang, MinFei Chu, Tongbin Zhong, CunDi Liu, JiaZheng |
| description | To explore the regulation relationship between miR-181a-3p and BMP10, and their mechanism of osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (MSCs).
After osteogenic induction of MSCs, the ALP activity was detected by ELISA. The expression of miRNA-181a-3p and BMP10 was detected by RT-qPCR, and the protein levels of BMP10 and osteogenic differentiation marker proteins ALK and RUNX2 were detected by Western blot. The TargetScan online website was used to predict the putative target of miR-181a-3p, and dual luciferase reporter assay was performed to validate the targeting relationship between miR-181a-3p and BMP10.
In osteogenic differentiation of MSCs, ALP activity, the level of ALK and RUNX2 was evidently increased (
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| doi_str_mv | 10.1080/21691401.2019.1687494 |
| format | Article |
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After osteogenic induction of MSCs, the ALP activity was detected by ELISA. The expression of miRNA-181a-3p and BMP10 was detected by RT-qPCR, and the protein levels of BMP10 and osteogenic differentiation marker proteins ALK and RUNX2 were detected by Western blot. The TargetScan online website was used to predict the putative target of miR-181a-3p, and dual luciferase reporter assay was performed to validate the targeting relationship between miR-181a-3p and BMP10.
In osteogenic differentiation of MSCs, ALP activity, the level of ALK and RUNX2 was evidently increased (
< .05), and the expression of miR-181a-3p was significantly downregulated (
< .05). Moreover, overexpression of miR-181a-3p obviously decreased the expression of BMP10 (
< .05), miR-181a-3p knockdown increased the expression of BMP10 prominently (
< .05). The transfection of miR-181a-3p mimics resulted in significantly downregulation of ALP activity and RUNX2 protein expression in MSCs (
< .05). In addition, overexpression of BMP10 could reverse the inhibitory effect of miR-181a-3p on osteogenic differentiation (
< .05).
In conclusion, we found that miR-181a-3p inhibited osteogenic differentiation of MCSs by targeting BMP10.]]></description><identifier>EISSN: 2169-141X</identifier><identifier>DOI: 10.1080/21691401.2019.1687494</identifier><identifier>PMID: 31713441</identifier><language>eng</language><publisher>England</publisher><subject>Base Sequence ; Bone Morphogenetic Proteins - genetics ; Cell Differentiation - genetics ; Gene Expression Regulation - genetics ; Humans ; Mesenchymal Stem Cells - cytology ; MicroRNAs - genetics ; Osteogenesis - genetics</subject><ispartof>Artificial cells, nanomedicine, and biotechnology, 2019-12, Vol.47 (1), p.4159-4164</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31713441$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tao, GuiLu</creatorcontrib><creatorcontrib>Mao, Ping</creatorcontrib><creatorcontrib>Guan, HaoNan</creatorcontrib><creatorcontrib>Jiang, MinFei</creatorcontrib><creatorcontrib>Chu, Tongbin</creatorcontrib><creatorcontrib>Zhong, CunDi</creatorcontrib><creatorcontrib>Liu, JiaZheng</creatorcontrib><title>Effect of miR-181a-3p on osteogenic differentiation of human bone marrow-derived mesenchymal stem cells by targeting BMP10</title><title>Artificial cells, nanomedicine, and biotechnology</title><addtitle>Artif Cells Nanomed Biotechnol</addtitle><description><![CDATA[To explore the regulation relationship between miR-181a-3p and BMP10, and their mechanism of osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (MSCs).
After osteogenic induction of MSCs, the ALP activity was detected by ELISA. The expression of miRNA-181a-3p and BMP10 was detected by RT-qPCR, and the protein levels of BMP10 and osteogenic differentiation marker proteins ALK and RUNX2 were detected by Western blot. The TargetScan online website was used to predict the putative target of miR-181a-3p, and dual luciferase reporter assay was performed to validate the targeting relationship between miR-181a-3p and BMP10.
In osteogenic differentiation of MSCs, ALP activity, the level of ALK and RUNX2 was evidently increased (
< .05), and the expression of miR-181a-3p was significantly downregulated (
< .05). Moreover, overexpression of miR-181a-3p obviously decreased the expression of BMP10 (
< .05), miR-181a-3p knockdown increased the expression of BMP10 prominently (
< .05). The transfection of miR-181a-3p mimics resulted in significantly downregulation of ALP activity and RUNX2 protein expression in MSCs (
< .05). In addition, overexpression of BMP10 could reverse the inhibitory effect of miR-181a-3p on osteogenic differentiation (
< .05).
In conclusion, we found that miR-181a-3p inhibited osteogenic differentiation of MCSs by targeting BMP10.]]></description><subject>Base Sequence</subject><subject>Bone Morphogenetic Proteins - genetics</subject><subject>Cell Differentiation - genetics</subject><subject>Gene Expression Regulation - genetics</subject><subject>Humans</subject><subject>Mesenchymal Stem Cells - cytology</subject><subject>MicroRNAs - genetics</subject><subject>Osteogenesis - genetics</subject><issn>2169-141X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kFtLAzEQhYMgttT-BCWPvmzNJNlLHrXUC1QUUfBtSbOzbWSzWTe7Sv31pqjzMjDn4zDnEHIGbAGsYJccMgWSwYIzUAvIilwqeUSmh3sCEt4mZB7CO4tTQJan8oRMBOQgpIQp-V7VNZqB-po6-5xAAToRHfUt9WFAv8XWGlrZCPXYDlYP9iDVdDc63dKNb5E63ff-K6mwt59YUYcBW7PbO93QaOGowaYJdLOng-63ONh2S68fnoCdkuNaNwHnf3tGXm9WL8u7ZP14e7-8WicdBxgSyYRhualiMMWwMKAyIxRmnEuRmiI3vDKm5iLqJtUikyoFKHitlABZGRAzcvHr2_X-Y8QwlM6Gw1O6RT-GkkcudsN4FtHzP3TcOKzKrrcx3b7870v8AAETa2c</recordid><startdate>20191204</startdate><enddate>20191204</enddate><creator>Tao, GuiLu</creator><creator>Mao, Ping</creator><creator>Guan, HaoNan</creator><creator>Jiang, MinFei</creator><creator>Chu, Tongbin</creator><creator>Zhong, CunDi</creator><creator>Liu, JiaZheng</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20191204</creationdate><title>Effect of miR-181a-3p on osteogenic differentiation of human bone marrow-derived mesenchymal stem cells by targeting BMP10</title><author>Tao, GuiLu ; Mao, Ping ; Guan, HaoNan ; Jiang, MinFei ; Chu, Tongbin ; Zhong, CunDi ; Liu, JiaZheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p211t-403c07cd68790e8c196c39e622435c87c2dccf23687c5a364951182f99314dc13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Base Sequence</topic><topic>Bone Morphogenetic Proteins - genetics</topic><topic>Cell Differentiation - genetics</topic><topic>Gene Expression Regulation - genetics</topic><topic>Humans</topic><topic>Mesenchymal Stem Cells - cytology</topic><topic>MicroRNAs - genetics</topic><topic>Osteogenesis - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tao, GuiLu</creatorcontrib><creatorcontrib>Mao, Ping</creatorcontrib><creatorcontrib>Guan, HaoNan</creatorcontrib><creatorcontrib>Jiang, MinFei</creatorcontrib><creatorcontrib>Chu, Tongbin</creatorcontrib><creatorcontrib>Zhong, CunDi</creatorcontrib><creatorcontrib>Liu, JiaZheng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Artificial cells, nanomedicine, and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tao, GuiLu</au><au>Mao, Ping</au><au>Guan, HaoNan</au><au>Jiang, MinFei</au><au>Chu, Tongbin</au><au>Zhong, CunDi</au><au>Liu, JiaZheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of miR-181a-3p on osteogenic differentiation of human bone marrow-derived mesenchymal stem cells by targeting BMP10</atitle><jtitle>Artificial cells, nanomedicine, and biotechnology</jtitle><addtitle>Artif Cells Nanomed Biotechnol</addtitle><date>2019-12-04</date><risdate>2019</risdate><volume>47</volume><issue>1</issue><spage>4159</spage><epage>4164</epage><pages>4159-4164</pages><eissn>2169-141X</eissn><abstract><![CDATA[To explore the regulation relationship between miR-181a-3p and BMP10, and their mechanism of osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (MSCs).
After osteogenic induction of MSCs, the ALP activity was detected by ELISA. The expression of miRNA-181a-3p and BMP10 was detected by RT-qPCR, and the protein levels of BMP10 and osteogenic differentiation marker proteins ALK and RUNX2 were detected by Western blot. The TargetScan online website was used to predict the putative target of miR-181a-3p, and dual luciferase reporter assay was performed to validate the targeting relationship between miR-181a-3p and BMP10.
In osteogenic differentiation of MSCs, ALP activity, the level of ALK and RUNX2 was evidently increased (
< .05), and the expression of miR-181a-3p was significantly downregulated (
< .05). Moreover, overexpression of miR-181a-3p obviously decreased the expression of BMP10 (
< .05), miR-181a-3p knockdown increased the expression of BMP10 prominently (
< .05). The transfection of miR-181a-3p mimics resulted in significantly downregulation of ALP activity and RUNX2 protein expression in MSCs (
< .05). In addition, overexpression of BMP10 could reverse the inhibitory effect of miR-181a-3p on osteogenic differentiation (
< .05).
In conclusion, we found that miR-181a-3p inhibited osteogenic differentiation of MCSs by targeting BMP10.]]></abstract><cop>England</cop><pmid>31713441</pmid><doi>10.1080/21691401.2019.1687494</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Artificial cells, nanomedicine, and biotechnology, 2019-12, Vol.47 (1), p.4159-4164 |
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| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Alma/SFX Local Collection |
| subjects | Base Sequence Bone Morphogenetic Proteins - genetics Cell Differentiation - genetics Gene Expression Regulation - genetics Humans Mesenchymal Stem Cells - cytology MicroRNAs - genetics Osteogenesis - genetics |
| title | Effect of miR-181a-3p on osteogenic differentiation of human bone marrow-derived mesenchymal stem cells by targeting BMP10 |
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