Hepatitis E virus infection in Hong Kong blood donors
Background and Objectives In Hong Kong, the dominant circulating hepatitis E virus (HEV) genotype is type 4, which can cause more severe clinical consequences than type 3. The aim of this study was to determine the HEV prevalence in Hong Kong blood donors. Materials and Methods Unlinked donation sam...
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| Veröffentlicht in: | Vox sanguinis 2020-01, Vol.115 (1), p.11-17 |
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| creator | Tsoi, Wai‐Chiu Zhu, Xiaomei To, Amanda Pui‐Chi Holmberg, Jerry |
| description | Background and Objectives
In Hong Kong, the dominant circulating hepatitis E virus (HEV) genotype is type 4, which can cause more severe clinical consequences than type 3. The aim of this study was to determine the HEV prevalence in Hong Kong blood donors.
Materials and Methods
Unlinked donation samples (n = 10 000) collected in March to May 2015 were tested for HEV RNA using the Procleix HEV assay in an individual donation format (IDT). A subset of 2000 samples were tested for IgG and IgM anti‐HEV using the Wantai enzyme‐linked immunosorbent assay (ELISA). Nucleic acid testing (NAT) initial reactive results were retested once, and repeatedly reactive donations were subjected to alternative molecular procedures as confirmation tests.
Results
One in 5000 Hong Kong blood donors was positive for HEV RNA (0·02%). The two RNA positive samples were also IgG and IgM anti‐HEV positive. One of the two RNA positive donors could be sequenced revealing genotype type 4. Anti‐HEV seroprevalence was estimated as 15·5% among all donors. IgG anti‐HEV positive rate for age group 16–20 was 3·1%, and it increased with age to 43·1% for age group 51–60. Sero‐positivity was higher in males (male donors 18·1% vs. female donors 13·2%), but it was mostly due to the difference in a specific age group (41–50).
Conclusion
Hepatitis E virus RNA positive rate of 0·02% was within the reported range of HEV RNA frequency in developed countries. One donor was confirmed to be genotype 4, which is the dominant genotype in circulation in Hong Kong. |
| doi_str_mv | 10.1111/vox.12846 |
| format | Article |
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In Hong Kong, the dominant circulating hepatitis E virus (HEV) genotype is type 4, which can cause more severe clinical consequences than type 3. The aim of this study was to determine the HEV prevalence in Hong Kong blood donors.
Materials and Methods
Unlinked donation samples (n = 10 000) collected in March to May 2015 were tested for HEV RNA using the Procleix HEV assay in an individual donation format (IDT). A subset of 2000 samples were tested for IgG and IgM anti‐HEV using the Wantai enzyme‐linked immunosorbent assay (ELISA). Nucleic acid testing (NAT) initial reactive results were retested once, and repeatedly reactive donations were subjected to alternative molecular procedures as confirmation tests.
Results
One in 5000 Hong Kong blood donors was positive for HEV RNA (0·02%). The two RNA positive samples were also IgG and IgM anti‐HEV positive. One of the two RNA positive donors could be sequenced revealing genotype type 4. Anti‐HEV seroprevalence was estimated as 15·5% among all donors. IgG anti‐HEV positive rate for age group 16–20 was 3·1%, and it increased with age to 43·1% for age group 51–60. Sero‐positivity was higher in males (male donors 18·1% vs. female donors 13·2%), but it was mostly due to the difference in a specific age group (41–50).
Conclusion
Hepatitis E virus RNA positive rate of 0·02% was within the reported range of HEV RNA frequency in developed countries. One donor was confirmed to be genotype 4, which is the dominant genotype in circulation in Hong Kong.</description><identifier>ISSN: 0042-9007</identifier><identifier>EISSN: 1423-0410</identifier><identifier>DOI: 10.1111/vox.12846</identifier><identifier>PMID: 31709559</identifier><language>eng</language><publisher>England: S. Karger AG</publisher><subject>Adolescent ; Adult ; Age ; Age Factors ; Age groups ; anti‐HEV IgG ; Blood & organ donations ; Blood circulation ; Blood Donors ; Developed countries ; Enzyme-Linked Immunosorbent Assay ; Female ; genotype ; Genotype & phenotype ; Genotypes ; Hepatitis ; Hepatitis Antibodies - blood ; Hepatitis E - epidemiology ; hepatitis E virus ; Hepatitis E virus - immunology ; Hong Kong - epidemiology ; Humans ; IgM ; Immunoglobulin G ; Immunoglobulin G - blood ; Immunoglobulin M ; Immunoglobulin M - blood ; Male ; Males ; Middle Aged ; Nucleic acids ; Prevalence ; Ribonucleic acid ; RNA ; RNA viruses ; RNA, Viral - blood ; Sampling methods ; Seroepidemiologic Studies ; Serology ; Viruses ; Young Adult</subject><ispartof>Vox sanguinis, 2020-01, Vol.115 (1), p.11-17</ispartof><rights>2019 International Society of Blood Transfusion</rights><rights>2019 International Society of Blood Transfusion.</rights><rights>Copyright Vox Sanguinis © 2020 International Society of Blood Transfusion</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3536-ab1c94f399f368996582c1d552e409e51490d96c3ddce2f5b4f916c25a6577883</citedby><cites>FETCH-LOGICAL-c3536-ab1c94f399f368996582c1d552e409e51490d96c3ddce2f5b4f916c25a6577883</cites><orcidid>0000-0003-2221-6833</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fvox.12846$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fvox.12846$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31709559$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsoi, Wai‐Chiu</creatorcontrib><creatorcontrib>Zhu, Xiaomei</creatorcontrib><creatorcontrib>To, Amanda Pui‐Chi</creatorcontrib><creatorcontrib>Holmberg, Jerry</creatorcontrib><title>Hepatitis E virus infection in Hong Kong blood donors</title><title>Vox sanguinis</title><addtitle>Vox Sang</addtitle><description>Background and Objectives
In Hong Kong, the dominant circulating hepatitis E virus (HEV) genotype is type 4, which can cause more severe clinical consequences than type 3. The aim of this study was to determine the HEV prevalence in Hong Kong blood donors.
Materials and Methods
Unlinked donation samples (n = 10 000) collected in March to May 2015 were tested for HEV RNA using the Procleix HEV assay in an individual donation format (IDT). A subset of 2000 samples were tested for IgG and IgM anti‐HEV using the Wantai enzyme‐linked immunosorbent assay (ELISA). Nucleic acid testing (NAT) initial reactive results were retested once, and repeatedly reactive donations were subjected to alternative molecular procedures as confirmation tests.
Results
One in 5000 Hong Kong blood donors was positive for HEV RNA (0·02%). The two RNA positive samples were also IgG and IgM anti‐HEV positive. One of the two RNA positive donors could be sequenced revealing genotype type 4. Anti‐HEV seroprevalence was estimated as 15·5% among all donors. IgG anti‐HEV positive rate for age group 16–20 was 3·1%, and it increased with age to 43·1% for age group 51–60. Sero‐positivity was higher in males (male donors 18·1% vs. female donors 13·2%), but it was mostly due to the difference in a specific age group (41–50).
Conclusion
Hepatitis E virus RNA positive rate of 0·02% was within the reported range of HEV RNA frequency in developed countries. One donor was confirmed to be genotype 4, which is the dominant genotype in circulation in Hong Kong.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Age</subject><subject>Age Factors</subject><subject>Age groups</subject><subject>anti‐HEV IgG</subject><subject>Blood & organ donations</subject><subject>Blood circulation</subject><subject>Blood Donors</subject><subject>Developed countries</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>genotype</subject><subject>Genotype & phenotype</subject><subject>Genotypes</subject><subject>Hepatitis</subject><subject>Hepatitis Antibodies - blood</subject><subject>Hepatitis E - epidemiology</subject><subject>hepatitis E virus</subject><subject>Hepatitis E virus - immunology</subject><subject>Hong Kong - epidemiology</subject><subject>Humans</subject><subject>IgM</subject><subject>Immunoglobulin G</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulin M</subject><subject>Immunoglobulin M - blood</subject><subject>Male</subject><subject>Males</subject><subject>Middle Aged</subject><subject>Nucleic acids</subject><subject>Prevalence</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA viruses</subject><subject>RNA, Viral - blood</subject><subject>Sampling methods</subject><subject>Seroepidemiologic Studies</subject><subject>Serology</subject><subject>Viruses</subject><subject>Young Adult</subject><issn>0042-9007</issn><issn>1423-0410</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10E9LwzAYBvAgipvTg19ACl700C3_mxxlTCcKu6h4C22aSkbXzKSd7tub2elBMIckhx_P-_IAcI7gGMUz2bjPMcKC8gMwRBSTFFIED8EQQopTCWE2ACchLCGEAgt2DAYEZVAyJoeAzc06b21rQzJLNtZ3IbFNZXRrXRN_ydw1b8nD7ipq58qkdI3z4RQcVXkdzNn-HYHn29nTdJ4-Lu7upzePqSaM8DQvkJa0IlJWhAspORNYo5IxbCiUhiEqYSm5JmWpDa5YQSuJuMYs5yzLhCAjcNXnrr1770xo1coGbeo6b4zrgsIEEc6pZCTSyz906TrfxO2iIpJyGcdFdd0r7V0I3lRq7e0q91uFoNp1qWKX6rvLaC_2iV2xMuWv_CkvgkkPPmxttv8nqZfFax_5BS8vet4</recordid><startdate>202001</startdate><enddate>202001</enddate><creator>Tsoi, Wai‐Chiu</creator><creator>Zhu, Xiaomei</creator><creator>To, Amanda Pui‐Chi</creator><creator>Holmberg, Jerry</creator><general>S. Karger AG</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7TM</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2221-6833</orcidid></search><sort><creationdate>202001</creationdate><title>Hepatitis E virus infection in Hong Kong blood donors</title><author>Tsoi, Wai‐Chiu ; Zhu, Xiaomei ; To, Amanda Pui‐Chi ; Holmberg, Jerry</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3536-ab1c94f399f368996582c1d552e409e51490d96c3ddce2f5b4f916c25a6577883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Age</topic><topic>Age Factors</topic><topic>Age groups</topic><topic>anti‐HEV IgG</topic><topic>Blood & organ donations</topic><topic>Blood circulation</topic><topic>Blood Donors</topic><topic>Developed countries</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>genotype</topic><topic>Genotype & phenotype</topic><topic>Genotypes</topic><topic>Hepatitis</topic><topic>Hepatitis Antibodies - blood</topic><topic>Hepatitis E - epidemiology</topic><topic>hepatitis E virus</topic><topic>Hepatitis E virus - immunology</topic><topic>Hong Kong - epidemiology</topic><topic>Humans</topic><topic>IgM</topic><topic>Immunoglobulin G</topic><topic>Immunoglobulin G - blood</topic><topic>Immunoglobulin M</topic><topic>Immunoglobulin M - blood</topic><topic>Male</topic><topic>Males</topic><topic>Middle Aged</topic><topic>Nucleic acids</topic><topic>Prevalence</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA viruses</topic><topic>RNA, Viral - blood</topic><topic>Sampling methods</topic><topic>Seroepidemiologic Studies</topic><topic>Serology</topic><topic>Viruses</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsoi, Wai‐Chiu</creatorcontrib><creatorcontrib>Zhu, Xiaomei</creatorcontrib><creatorcontrib>To, Amanda Pui‐Chi</creatorcontrib><creatorcontrib>Holmberg, Jerry</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Vox sanguinis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsoi, Wai‐Chiu</au><au>Zhu, Xiaomei</au><au>To, Amanda Pui‐Chi</au><au>Holmberg, Jerry</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hepatitis E virus infection in Hong Kong blood donors</atitle><jtitle>Vox sanguinis</jtitle><addtitle>Vox Sang</addtitle><date>2020-01</date><risdate>2020</risdate><volume>115</volume><issue>1</issue><spage>11</spage><epage>17</epage><pages>11-17</pages><issn>0042-9007</issn><eissn>1423-0410</eissn><abstract>Background and Objectives
In Hong Kong, the dominant circulating hepatitis E virus (HEV) genotype is type 4, which can cause more severe clinical consequences than type 3. The aim of this study was to determine the HEV prevalence in Hong Kong blood donors.
Materials and Methods
Unlinked donation samples (n = 10 000) collected in March to May 2015 were tested for HEV RNA using the Procleix HEV assay in an individual donation format (IDT). A subset of 2000 samples were tested for IgG and IgM anti‐HEV using the Wantai enzyme‐linked immunosorbent assay (ELISA). Nucleic acid testing (NAT) initial reactive results were retested once, and repeatedly reactive donations were subjected to alternative molecular procedures as confirmation tests.
Results
One in 5000 Hong Kong blood donors was positive for HEV RNA (0·02%). The two RNA positive samples were also IgG and IgM anti‐HEV positive. One of the two RNA positive donors could be sequenced revealing genotype type 4. Anti‐HEV seroprevalence was estimated as 15·5% among all donors. IgG anti‐HEV positive rate for age group 16–20 was 3·1%, and it increased with age to 43·1% for age group 51–60. Sero‐positivity was higher in males (male donors 18·1% vs. female donors 13·2%), but it was mostly due to the difference in a specific age group (41–50).
Conclusion
Hepatitis E virus RNA positive rate of 0·02% was within the reported range of HEV RNA frequency in developed countries. One donor was confirmed to be genotype 4, which is the dominant genotype in circulation in Hong Kong.</abstract><cop>England</cop><pub>S. Karger AG</pub><pmid>31709559</pmid><doi>10.1111/vox.12846</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-2221-6833</orcidid></addata></record> |
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| ispartof | Vox sanguinis, 2020-01, Vol.115 (1), p.11-17 |
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| subjects | Adolescent Adult Age Age Factors Age groups anti‐HEV IgG Blood & organ donations Blood circulation Blood Donors Developed countries Enzyme-Linked Immunosorbent Assay Female genotype Genotype & phenotype Genotypes Hepatitis Hepatitis Antibodies - blood Hepatitis E - epidemiology hepatitis E virus Hepatitis E virus - immunology Hong Kong - epidemiology Humans IgM Immunoglobulin G Immunoglobulin G - blood Immunoglobulin M Immunoglobulin M - blood Male Males Middle Aged Nucleic acids Prevalence Ribonucleic acid RNA RNA viruses RNA, Viral - blood Sampling methods Seroepidemiologic Studies Serology Viruses Young Adult |
| title | Hepatitis E virus infection in Hong Kong blood donors |
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