Ameliorative effect of combined low dose of Pioglitazone and omega‐3 on spermatogenesis and steroidogenesis in diabetic rats
Background Chronic hyperglycemia is linked to either subfertility or infertility among diabetic males. Pioglitazone is one of the thiazolidinediones (TZDs) drugs that are selective peroxisome proliferator‐activated receptor (PPAR‐γ agonists used for treating type 2 diabetes mellitus (T2DM). Aim This...
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| Veröffentlicht in: | Journal of cellular biochemistry 2020-02, Vol.121 (2), p.1524-1540 |
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| creator | Hasan, Mai M. El‐Shal, Amal S. Mackawy, Amal M. H. Ibrahim, Ebtesam M. Abdelghany, Eman M. M. A. Saeed, Abeer A. El‐Gendy, Jehan |
| description | Background
Chronic hyperglycemia is linked to either subfertility or infertility among diabetic males. Pioglitazone is one of the thiazolidinediones (TZDs) drugs that are selective peroxisome proliferator‐activated receptor (PPAR‐γ agonists used for treating type 2 diabetes mellitus (T2DM).
Aim
This study aims to explore the possible effect of low Pioglitazone dose and omega (ω‐3) on rat male reproductive function. Furthermore, we evaluated the add‐on effect of combined use of low Pioglitazone dose of and ω‐3 on reproductive functions in adult male T2DM rats.
Methods
Fifty adult male rats were included and subdivided into control and four test subgroups. T2DM was induced in test groups and subdivided into non‐treated T2DM, ω‐3 treated, 0.6 mg/kg Pioglitazone treated, and combined treated group (orally by gavage). Following 16 weeks, final body weight, testicular weight, fasting plasma glucose, and serum testosterone levels were measured. Semen analysis, testicular testosterone, malondialdehyde (MDA) concentrations, superoxide dismutase (SOD) activity, immunohistochemistry staining for apoptosis marker B‐cell lymphoma protein 2 (Bcl‐2), proliferation marker as proliferating cell nuclear antigen (PCNA), estrogen receptor α (ERα), androgen receptor (AR) were determined. Caspase‐3, nuclear factor‐kappa B (NF‐kB), glucose transporter 3 (GLUT3), 17β‐hydroxysteroid dehydrogenases (17β‐HSD) PPARγ, and PPARα genes expression were analyzed by real‐time polymerase chain reaction (RT‐PCR).
Results
Our findings revealed that treatment with low dose of Pioglitazone or ω‐3 significantly lowered fasting plasma glucose and MDA levels, ameliorated diabetes effects on histological damage, improved antioxidant activity (SOD), significantly improved anti‐apoptosis BCL‐2 and proliferation (PCNA), remarkably elevated ERα, AR, 17β‐HSD PPARγ, and PPARα expression with significant reduction in caspase‐3, NF‐kB genes expression and improved semen quality as well. Combined use of low dose of and ω‐3 has better effects on all measured parameters.
Conclusion
Small Pioglitazone dose and ω‐3 possess beneficial effects on spermatogenic and steroidogenic functions in adult diabetic rat; while combined use of both has an add‐on effect.
Combined use of low dose of and omega‐3 has better effects on all measured parameters. Thus, small dose of and omega‐3 fatty acids possess beneficial effects on spermatogenic and steroidogenic functions in adult diabetic rat; notably use of both has an add‐on |
| doi_str_mv | 10.1002/jcb.29388 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2313656195</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2313656195</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3538-a6f1c918e48f2535cb1972cc840e8ae55ec2d9da28cae5d884bd6676681ead643</originalsourceid><addsrcrecordid>eNp10ctKxDAUBuAgio6XhS8gATe6qObSpslSB68IutB1SZPTIUPbjElH0YX4CD6jT2J01IXgKuTw8SecH6FtSg4oIexwauoDpriUS2hEiSqzXOT5MhqRkpOMccrW0HqMU0KIUpytojVOS6IEpyP0ctRB63zQg3sADE0DZsC-wcZ3tevB4tY_YusjfA5vnJ-0btDPvgese4t9BxP9_vrGse9xnEHo9OAn0EN08QvEAYJ39nfmemydrmFwBqc34yZaaXQbYev73EB3pye34_Ps6vrsYnx0lRlecJlp0VCjqIRcNqzghampKpkxMicgNRQFGGaV1UyadLNS5rUVohRCUtBW5HwD7S1yZ8HfzyEOVeeigbbVPfh5rNKSuCgEVUWiu3_o1M9Dn36XFCu5TFskSe0vlAk-xgBNNQuu0-GpoqT6LKVKpVRfpSS78504rzuwv_KnhQQOF-DRtfD0f1J1OT5eRH4Ag7SYJA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2327380090</pqid></control><display><type>article</type><title>Ameliorative effect of combined low dose of Pioglitazone and omega‐3 on spermatogenesis and steroidogenesis in diabetic rats</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Hasan, Mai M. ; El‐Shal, Amal S. ; Mackawy, Amal M. H. ; Ibrahim, Ebtesam M. ; Abdelghany, Eman M. M. A. ; Saeed, Abeer A. ; El‐Gendy, Jehan</creator><creatorcontrib>Hasan, Mai M. ; El‐Shal, Amal S. ; Mackawy, Amal M. H. ; Ibrahim, Ebtesam M. ; Abdelghany, Eman M. M. A. ; Saeed, Abeer A. ; El‐Gendy, Jehan</creatorcontrib><description>Background
Chronic hyperglycemia is linked to either subfertility or infertility among diabetic males. Pioglitazone is one of the thiazolidinediones (TZDs) drugs that are selective peroxisome proliferator‐activated receptor (PPAR‐γ agonists used for treating type 2 diabetes mellitus (T2DM).
Aim
This study aims to explore the possible effect of low Pioglitazone dose and omega (ω‐3) on rat male reproductive function. Furthermore, we evaluated the add‐on effect of combined use of low Pioglitazone dose of and ω‐3 on reproductive functions in adult male T2DM rats.
Methods
Fifty adult male rats were included and subdivided into control and four test subgroups. T2DM was induced in test groups and subdivided into non‐treated T2DM, ω‐3 treated, 0.6 mg/kg Pioglitazone treated, and combined treated group (orally by gavage). Following 16 weeks, final body weight, testicular weight, fasting plasma glucose, and serum testosterone levels were measured. Semen analysis, testicular testosterone, malondialdehyde (MDA) concentrations, superoxide dismutase (SOD) activity, immunohistochemistry staining for apoptosis marker B‐cell lymphoma protein 2 (Bcl‐2), proliferation marker as proliferating cell nuclear antigen (PCNA), estrogen receptor α (ERα), androgen receptor (AR) were determined. Caspase‐3, nuclear factor‐kappa B (NF‐kB), glucose transporter 3 (GLUT3), 17β‐hydroxysteroid dehydrogenases (17β‐HSD) PPARγ, and PPARα genes expression were analyzed by real‐time polymerase chain reaction (RT‐PCR).
Results
Our findings revealed that treatment with low dose of Pioglitazone or ω‐3 significantly lowered fasting plasma glucose and MDA levels, ameliorated diabetes effects on histological damage, improved antioxidant activity (SOD), significantly improved anti‐apoptosis BCL‐2 and proliferation (PCNA), remarkably elevated ERα, AR, 17β‐HSD PPARγ, and PPARα expression with significant reduction in caspase‐3, NF‐kB genes expression and improved semen quality as well. Combined use of low dose of and ω‐3 has better effects on all measured parameters.
Conclusion
Small Pioglitazone dose and ω‐3 possess beneficial effects on spermatogenic and steroidogenic functions in adult diabetic rat; while combined use of both has an add‐on effect.
Combined use of low dose of and omega‐3 has better effects on all measured parameters. Thus, small dose of and omega‐3 fatty acids possess beneficial effects on spermatogenic and steroidogenic functions in adult diabetic rat; notably use of both has an add‐on effect.</description><identifier>ISSN: 0730-2312</identifier><identifier>EISSN: 1097-4644</identifier><identifier>DOI: 10.1002/jcb.29388</identifier><identifier>PMID: 31709631</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Androgen receptors ; Animals ; Antigens ; Antioxidants ; Apoptosis ; Beta cells ; Body weight ; Caspase ; Diabetes ; Diabetes mellitus ; Diabetes mellitus (non-insulin dependent) ; Diabetes Mellitus, Experimental - complications ; Diabetes Mellitus, Experimental - pathology ; Diabetes Mellitus, Type 2 - complications ; Diabetes Mellitus, Type 2 - pathology ; Dose-Response Relationship, Drug ; Drug Therapy, Combination ; Estrogens ; Fasting ; Fatty Acids, Omega-3 - administration & dosage ; Fatty Acids, Omega-3 - pharmacology ; Fertility ; Gene expression ; Genes ; Glucose ; Glucose transporter ; Hyperglycemia ; Hypoglycemic Agents - pharmacology ; Immunohistochemistry ; Infertility ; Infertility, Male - drug therapy ; Infertility, Male - etiology ; Infertility, Male - metabolism ; Infertility, Male - pathology ; Lymphoma ; Male ; Males ; Malondialdehyde ; Markers ; omega‐3 ; Peroxisome proliferator-activated receptors ; Pioglitazone ; Pioglitazone - administration & dosage ; Pioglitazone - pharmacology ; Polymerase chain reaction ; PPAR ; PPAR alpha ; Proliferating cell nuclear antigen ; rat male fertility ; Rats ; real‐time polymerase chain reaction ; Semen ; Semen Analysis ; Spermatogenesis ; Steroidogenesis ; Subgroups ; Superoxide dismutase ; Testes ; Testis - drug effects ; Testis - metabolism ; Testis - pathology ; Testosterone ; Testosterone - metabolism ; Thiazolidinediones</subject><ispartof>Journal of cellular biochemistry, 2020-02, Vol.121 (2), p.1524-1540</ispartof><rights>2019 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3538-a6f1c918e48f2535cb1972cc840e8ae55ec2d9da28cae5d884bd6676681ead643</citedby><cites>FETCH-LOGICAL-c3538-a6f1c918e48f2535cb1972cc840e8ae55ec2d9da28cae5d884bd6676681ead643</cites><orcidid>0000-0003-2167-8841</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcb.29388$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcb.29388$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31709631$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hasan, Mai M.</creatorcontrib><creatorcontrib>El‐Shal, Amal S.</creatorcontrib><creatorcontrib>Mackawy, Amal M. H.</creatorcontrib><creatorcontrib>Ibrahim, Ebtesam M.</creatorcontrib><creatorcontrib>Abdelghany, Eman M. M. A.</creatorcontrib><creatorcontrib>Saeed, Abeer A.</creatorcontrib><creatorcontrib>El‐Gendy, Jehan</creatorcontrib><title>Ameliorative effect of combined low dose of Pioglitazone and omega‐3 on spermatogenesis and steroidogenesis in diabetic rats</title><title>Journal of cellular biochemistry</title><addtitle>J Cell Biochem</addtitle><description>Background
Chronic hyperglycemia is linked to either subfertility or infertility among diabetic males. Pioglitazone is one of the thiazolidinediones (TZDs) drugs that are selective peroxisome proliferator‐activated receptor (PPAR‐γ agonists used for treating type 2 diabetes mellitus (T2DM).
Aim
This study aims to explore the possible effect of low Pioglitazone dose and omega (ω‐3) on rat male reproductive function. Furthermore, we evaluated the add‐on effect of combined use of low Pioglitazone dose of and ω‐3 on reproductive functions in adult male T2DM rats.
Methods
Fifty adult male rats were included and subdivided into control and four test subgroups. T2DM was induced in test groups and subdivided into non‐treated T2DM, ω‐3 treated, 0.6 mg/kg Pioglitazone treated, and combined treated group (orally by gavage). Following 16 weeks, final body weight, testicular weight, fasting plasma glucose, and serum testosterone levels were measured. Semen analysis, testicular testosterone, malondialdehyde (MDA) concentrations, superoxide dismutase (SOD) activity, immunohistochemistry staining for apoptosis marker B‐cell lymphoma protein 2 (Bcl‐2), proliferation marker as proliferating cell nuclear antigen (PCNA), estrogen receptor α (ERα), androgen receptor (AR) were determined. Caspase‐3, nuclear factor‐kappa B (NF‐kB), glucose transporter 3 (GLUT3), 17β‐hydroxysteroid dehydrogenases (17β‐HSD) PPARγ, and PPARα genes expression were analyzed by real‐time polymerase chain reaction (RT‐PCR).
Results
Our findings revealed that treatment with low dose of Pioglitazone or ω‐3 significantly lowered fasting plasma glucose and MDA levels, ameliorated diabetes effects on histological damage, improved antioxidant activity (SOD), significantly improved anti‐apoptosis BCL‐2 and proliferation (PCNA), remarkably elevated ERα, AR, 17β‐HSD PPARγ, and PPARα expression with significant reduction in caspase‐3, NF‐kB genes expression and improved semen quality as well. Combined use of low dose of and ω‐3 has better effects on all measured parameters.
Conclusion
Small Pioglitazone dose and ω‐3 possess beneficial effects on spermatogenic and steroidogenic functions in adult diabetic rat; while combined use of both has an add‐on effect.
Combined use of low dose of and omega‐3 has better effects on all measured parameters. Thus, small dose of and omega‐3 fatty acids possess beneficial effects on spermatogenic and steroidogenic functions in adult diabetic rat; notably use of both has an add‐on effect.</description><subject>Androgen receptors</subject><subject>Animals</subject><subject>Antigens</subject><subject>Antioxidants</subject><subject>Apoptosis</subject><subject>Beta cells</subject><subject>Body weight</subject><subject>Caspase</subject><subject>Diabetes</subject><subject>Diabetes mellitus</subject><subject>Diabetes mellitus (non-insulin dependent)</subject><subject>Diabetes Mellitus, Experimental - complications</subject><subject>Diabetes Mellitus, Experimental - pathology</subject><subject>Diabetes Mellitus, Type 2 - complications</subject><subject>Diabetes Mellitus, Type 2 - pathology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug Therapy, Combination</subject><subject>Estrogens</subject><subject>Fasting</subject><subject>Fatty Acids, Omega-3 - administration & dosage</subject><subject>Fatty Acids, Omega-3 - pharmacology</subject><subject>Fertility</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Glucose</subject><subject>Glucose transporter</subject><subject>Hyperglycemia</subject><subject>Hypoglycemic Agents - pharmacology</subject><subject>Immunohistochemistry</subject><subject>Infertility</subject><subject>Infertility, Male - drug therapy</subject><subject>Infertility, Male - etiology</subject><subject>Infertility, Male - metabolism</subject><subject>Infertility, Male - pathology</subject><subject>Lymphoma</subject><subject>Male</subject><subject>Males</subject><subject>Malondialdehyde</subject><subject>Markers</subject><subject>omega‐3</subject><subject>Peroxisome proliferator-activated receptors</subject><subject>Pioglitazone</subject><subject>Pioglitazone - administration & dosage</subject><subject>Pioglitazone - pharmacology</subject><subject>Polymerase chain reaction</subject><subject>PPAR</subject><subject>PPAR alpha</subject><subject>Proliferating cell nuclear antigen</subject><subject>rat male fertility</subject><subject>Rats</subject><subject>real‐time polymerase chain reaction</subject><subject>Semen</subject><subject>Semen Analysis</subject><subject>Spermatogenesis</subject><subject>Steroidogenesis</subject><subject>Subgroups</subject><subject>Superoxide dismutase</subject><subject>Testes</subject><subject>Testis - drug effects</subject><subject>Testis - metabolism</subject><subject>Testis - pathology</subject><subject>Testosterone</subject><subject>Testosterone - metabolism</subject><subject>Thiazolidinediones</subject><issn>0730-2312</issn><issn>1097-4644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10ctKxDAUBuAgio6XhS8gATe6qObSpslSB68IutB1SZPTIUPbjElH0YX4CD6jT2J01IXgKuTw8SecH6FtSg4oIexwauoDpriUS2hEiSqzXOT5MhqRkpOMccrW0HqMU0KIUpytojVOS6IEpyP0ctRB63zQg3sADE0DZsC-wcZ3tevB4tY_YusjfA5vnJ-0btDPvgese4t9BxP9_vrGse9xnEHo9OAn0EN08QvEAYJ39nfmemydrmFwBqc34yZaaXQbYev73EB3pye34_Ps6vrsYnx0lRlecJlp0VCjqIRcNqzghampKpkxMicgNRQFGGaV1UyadLNS5rUVohRCUtBW5HwD7S1yZ8HfzyEOVeeigbbVPfh5rNKSuCgEVUWiu3_o1M9Dn36XFCu5TFskSe0vlAk-xgBNNQuu0-GpoqT6LKVKpVRfpSS78504rzuwv_KnhQQOF-DRtfD0f1J1OT5eRH4Ag7SYJA</recordid><startdate>202002</startdate><enddate>202002</enddate><creator>Hasan, Mai M.</creator><creator>El‐Shal, Amal S.</creator><creator>Mackawy, Amal M. H.</creator><creator>Ibrahim, Ebtesam M.</creator><creator>Abdelghany, Eman M. M. A.</creator><creator>Saeed, Abeer A.</creator><creator>El‐Gendy, Jehan</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2167-8841</orcidid></search><sort><creationdate>202002</creationdate><title>Ameliorative effect of combined low dose of Pioglitazone and omega‐3 on spermatogenesis and steroidogenesis in diabetic rats</title><author>Hasan, Mai M. ; El‐Shal, Amal S. ; Mackawy, Amal M. H. ; Ibrahim, Ebtesam M. ; Abdelghany, Eman M. M. A. ; Saeed, Abeer A. ; El‐Gendy, Jehan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3538-a6f1c918e48f2535cb1972cc840e8ae55ec2d9da28cae5d884bd6676681ead643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Androgen receptors</topic><topic>Animals</topic><topic>Antigens</topic><topic>Antioxidants</topic><topic>Apoptosis</topic><topic>Beta cells</topic><topic>Body weight</topic><topic>Caspase</topic><topic>Diabetes</topic><topic>Diabetes mellitus</topic><topic>Diabetes mellitus (non-insulin dependent)</topic><topic>Diabetes Mellitus, Experimental - complications</topic><topic>Diabetes Mellitus, Experimental - pathology</topic><topic>Diabetes Mellitus, Type 2 - complications</topic><topic>Diabetes Mellitus, Type 2 - pathology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drug Therapy, Combination</topic><topic>Estrogens</topic><topic>Fasting</topic><topic>Fatty Acids, Omega-3 - administration & dosage</topic><topic>Fatty Acids, Omega-3 - pharmacology</topic><topic>Fertility</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Glucose</topic><topic>Glucose transporter</topic><topic>Hyperglycemia</topic><topic>Hypoglycemic Agents - pharmacology</topic><topic>Immunohistochemistry</topic><topic>Infertility</topic><topic>Infertility, Male - drug therapy</topic><topic>Infertility, Male - etiology</topic><topic>Infertility, Male - metabolism</topic><topic>Infertility, Male - pathology</topic><topic>Lymphoma</topic><topic>Male</topic><topic>Males</topic><topic>Malondialdehyde</topic><topic>Markers</topic><topic>omega‐3</topic><topic>Peroxisome proliferator-activated receptors</topic><topic>Pioglitazone</topic><topic>Pioglitazone - administration & dosage</topic><topic>Pioglitazone - pharmacology</topic><topic>Polymerase chain reaction</topic><topic>PPAR</topic><topic>PPAR alpha</topic><topic>Proliferating cell nuclear antigen</topic><topic>rat male fertility</topic><topic>Rats</topic><topic>real‐time polymerase chain reaction</topic><topic>Semen</topic><topic>Semen Analysis</topic><topic>Spermatogenesis</topic><topic>Steroidogenesis</topic><topic>Subgroups</topic><topic>Superoxide dismutase</topic><topic>Testes</topic><topic>Testis - drug effects</topic><topic>Testis - metabolism</topic><topic>Testis - pathology</topic><topic>Testosterone</topic><topic>Testosterone - metabolism</topic><topic>Thiazolidinediones</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hasan, Mai M.</creatorcontrib><creatorcontrib>El‐Shal, Amal S.</creatorcontrib><creatorcontrib>Mackawy, Amal M. H.</creatorcontrib><creatorcontrib>Ibrahim, Ebtesam M.</creatorcontrib><creatorcontrib>Abdelghany, Eman M. M. A.</creatorcontrib><creatorcontrib>Saeed, Abeer A.</creatorcontrib><creatorcontrib>El‐Gendy, Jehan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hasan, Mai M.</au><au>El‐Shal, Amal S.</au><au>Mackawy, Amal M. H.</au><au>Ibrahim, Ebtesam M.</au><au>Abdelghany, Eman M. M. A.</au><au>Saeed, Abeer A.</au><au>El‐Gendy, Jehan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ameliorative effect of combined low dose of Pioglitazone and omega‐3 on spermatogenesis and steroidogenesis in diabetic rats</atitle><jtitle>Journal of cellular biochemistry</jtitle><addtitle>J Cell Biochem</addtitle><date>2020-02</date><risdate>2020</risdate><volume>121</volume><issue>2</issue><spage>1524</spage><epage>1540</epage><pages>1524-1540</pages><issn>0730-2312</issn><eissn>1097-4644</eissn><abstract>Background
Chronic hyperglycemia is linked to either subfertility or infertility among diabetic males. Pioglitazone is one of the thiazolidinediones (TZDs) drugs that are selective peroxisome proliferator‐activated receptor (PPAR‐γ agonists used for treating type 2 diabetes mellitus (T2DM).
Aim
This study aims to explore the possible effect of low Pioglitazone dose and omega (ω‐3) on rat male reproductive function. Furthermore, we evaluated the add‐on effect of combined use of low Pioglitazone dose of and ω‐3 on reproductive functions in adult male T2DM rats.
Methods
Fifty adult male rats were included and subdivided into control and four test subgroups. T2DM was induced in test groups and subdivided into non‐treated T2DM, ω‐3 treated, 0.6 mg/kg Pioglitazone treated, and combined treated group (orally by gavage). Following 16 weeks, final body weight, testicular weight, fasting plasma glucose, and serum testosterone levels were measured. Semen analysis, testicular testosterone, malondialdehyde (MDA) concentrations, superoxide dismutase (SOD) activity, immunohistochemistry staining for apoptosis marker B‐cell lymphoma protein 2 (Bcl‐2), proliferation marker as proliferating cell nuclear antigen (PCNA), estrogen receptor α (ERα), androgen receptor (AR) were determined. Caspase‐3, nuclear factor‐kappa B (NF‐kB), glucose transporter 3 (GLUT3), 17β‐hydroxysteroid dehydrogenases (17β‐HSD) PPARγ, and PPARα genes expression were analyzed by real‐time polymerase chain reaction (RT‐PCR).
Results
Our findings revealed that treatment with low dose of Pioglitazone or ω‐3 significantly lowered fasting plasma glucose and MDA levels, ameliorated diabetes effects on histological damage, improved antioxidant activity (SOD), significantly improved anti‐apoptosis BCL‐2 and proliferation (PCNA), remarkably elevated ERα, AR, 17β‐HSD PPARγ, and PPARα expression with significant reduction in caspase‐3, NF‐kB genes expression and improved semen quality as well. Combined use of low dose of and ω‐3 has better effects on all measured parameters.
Conclusion
Small Pioglitazone dose and ω‐3 possess beneficial effects on spermatogenic and steroidogenic functions in adult diabetic rat; while combined use of both has an add‐on effect.
Combined use of low dose of and omega‐3 has better effects on all measured parameters. Thus, small dose of and omega‐3 fatty acids possess beneficial effects on spermatogenic and steroidogenic functions in adult diabetic rat; notably use of both has an add‐on effect.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31709631</pmid><doi>10.1002/jcb.29388</doi><tpages>17</tpages><orcidid>https://orcid.org/0000-0003-2167-8841</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0730-2312 |
| ispartof | Journal of cellular biochemistry, 2020-02, Vol.121 (2), p.1524-1540 |
| issn | 0730-2312 1097-4644 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2313656195 |
| source | MEDLINE; Access via Wiley Online Library |
| subjects | Androgen receptors Animals Antigens Antioxidants Apoptosis Beta cells Body weight Caspase Diabetes Diabetes mellitus Diabetes mellitus (non-insulin dependent) Diabetes Mellitus, Experimental - complications Diabetes Mellitus, Experimental - pathology Diabetes Mellitus, Type 2 - complications Diabetes Mellitus, Type 2 - pathology Dose-Response Relationship, Drug Drug Therapy, Combination Estrogens Fasting Fatty Acids, Omega-3 - administration & dosage Fatty Acids, Omega-3 - pharmacology Fertility Gene expression Genes Glucose Glucose transporter Hyperglycemia Hypoglycemic Agents - pharmacology Immunohistochemistry Infertility Infertility, Male - drug therapy Infertility, Male - etiology Infertility, Male - metabolism Infertility, Male - pathology Lymphoma Male Males Malondialdehyde Markers omega‐3 Peroxisome proliferator-activated receptors Pioglitazone Pioglitazone - administration & dosage Pioglitazone - pharmacology Polymerase chain reaction PPAR PPAR alpha Proliferating cell nuclear antigen rat male fertility Rats real‐time polymerase chain reaction Semen Semen Analysis Spermatogenesis Steroidogenesis Subgroups Superoxide dismutase Testes Testis - drug effects Testis - metabolism Testis - pathology Testosterone Testosterone - metabolism Thiazolidinediones |
| title | Ameliorative effect of combined low dose of Pioglitazone and omega‐3 on spermatogenesis and steroidogenesis in diabetic rats |
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