N-acetyl-l-cysteine protects porcine oocytes undergoing meiotic resumption from heat stress

•GVBD oocytes undergoing meiotic resumption are much sensitive to HS.•GVBD-specific HS disorganizes oocyte spindle assembly and inhibits ERK signaling.•GVBD-specific HS induces erroneous H3K27me3 modification in oocytes.•NAC protects oocyte cellular and molecular events against GVBD-specific HS.•NAC...

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Veröffentlicht in:Reproductive toxicology (Elmsford, N.Y.) N.Y.), 2020-01, Vol.91, p.27-34
Hauptverfasser: Hu, Xiao, Cheng, Linghua, Wang, Xiaodong, Luo, Gang, Zhao, Tianqing, Tian, Jianhui, An, Lei
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container_title Reproductive toxicology (Elmsford, N.Y.)
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creator Hu, Xiao
Cheng, Linghua
Wang, Xiaodong
Luo, Gang
Zhao, Tianqing
Tian, Jianhui
An, Lei
description •GVBD oocytes undergoing meiotic resumption are much sensitive to HS.•GVBD-specific HS disorganizes oocyte spindle assembly and inhibits ERK signaling.•GVBD-specific HS induces erroneous H3K27me3 modification in oocytes.•NAC protects oocyte cellular and molecular events against GVBD-specific HS.•NAC rescues impaired oocyte developmental potential following GVBD-specific HS. Heat stress (HS) is a notable risk factor for female reproductive performance. In particular, impaired oocyte maturation was thought to contribute largely to the HS-induced reproductive dysfunctions. In this study, we confirmed that oocytes undergoing GVBD were much susceptible to HS, and thus compromising subsequent embryonic development. Using N-acetyl-l-cysteine (NAC), we found supplementation of a relatively high dose NAC during in vitro maturation, can protect oocytes from HS-induced complications, and thus rescuing impaired embryonic development. Further analysis indicated that mechanisms responsible for protecting GVBD oocytes from HS by NAC may include: (1) reversing disorganized spindle assembly and inhibited extracellular signal–regulated kinase (ERK) signaling; (2) correcting erroneous H3K27me3 modification and dysregulated expression of imprinted genes; (3) alleviating increased intraoocyte reactive oxygen species accumulation and apoptosis initiation. Our study, focusing on the oocyte meiotic maturation, may provide a safe and promising strategy for protecting reproductive sows under environmental hyperthermal conditions.
doi_str_mv 10.1016/j.reprotox.2019.10.006
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Heat stress (HS) is a notable risk factor for female reproductive performance. In particular, impaired oocyte maturation was thought to contribute largely to the HS-induced reproductive dysfunctions. In this study, we confirmed that oocytes undergoing GVBD were much susceptible to HS, and thus compromising subsequent embryonic development. Using N-acetyl-l-cysteine (NAC), we found supplementation of a relatively high dose NAC during in vitro maturation, can protect oocytes from HS-induced complications, and thus rescuing impaired embryonic development. Further analysis indicated that mechanisms responsible for protecting GVBD oocytes from HS by NAC may include: (1) reversing disorganized spindle assembly and inhibited extracellular signal–regulated kinase (ERK) signaling; (2) correcting erroneous H3K27me3 modification and dysregulated expression of imprinted genes; (3) alleviating increased intraoocyte reactive oxygen species accumulation and apoptosis initiation. 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subjects Acetylcysteine - pharmacology
Animals
Apoptosis
Embryonic development
Female
Heat stress
Heat-Shock Response
In Vitro Oocyte Maturation Techniques
Life Sciences & Biomedicine
Meiosis
N-acetyl-l-cysteine
Oocyte maturation
Oocytes - cytology
Oocytes - drug effects
Oocytes - metabolism
Oxidative Stress
Porcine oocytes
Protective Agents - pharmacology
Reactive Oxygen Species - metabolism
Reproductive Biology
Science & Technology
Swine
Toxicology
title N-acetyl-l-cysteine protects porcine oocytes undergoing meiotic resumption from heat stress
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