Antinociceptive effects of treadmill exercise in a rat model of Parkinson's disease: The role of cannabinoid and opioid receptors
•Treadmill exercise promote antinociceptive effects in a rat model of Parkinson's disease.•Pain threshold improvement after treadmill exercise protocol in a 6-OHDA rat model of Parkinsońs disease.•Cannabinoids and opioids receptors role on antinociceptive effects in a rat model of Parkinsońs di...
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| Veröffentlicht in: | Brain research 2020-01, Vol.1727, p.146521-146521, Article 146521 |
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| creator | Binda, K.H. Real, C.C. Ferreira, A.F.F. Britto, L.R. Chacur, M. |
| description | •Treadmill exercise promote antinociceptive effects in a rat model of Parkinson's disease.•Pain threshold improvement after treadmill exercise protocol in a 6-OHDA rat model of Parkinsońs disease.•Cannabinoids and opioids receptors role on antinociceptive effects in a rat model of Parkinsońs disease.
In addition to motor symptoms, Parkinson's disease (PD) presents high prevalence of painful symptoms responsible for worsening quality of life of PD patients. Physical exercise can improve such painful symptoms. This study evaluated the effects of exercise on nociceptive threshold using an unilateral rat model of PD, as well as the role played by cannabinoid and opioid receptors in areas responsible for pain pathways.
For PD induction, Wistar rats were injected with 6-OHDA. 15 days after, rats either remained sedentary or were forced to exercise three times a week for 40 min. Motor and nociceptive behaviors were evaluated through cylinder and mechanical hyperalgesia tests, respectively. The animals were euthanized for analysis of cannabinoid receptor type 1 (CB1) and type 2 (CB2), and μ-opioid receptor (MOR) in the anterior cingulate cortex (ACC), periaqueductal gray matter (PAG), and thalamus areas by immunohistochemistry (IHC) and Western blotting.
Our data revealed a decrease in the nociceptive threshold in both forepaws after surgery; in contrast, there was improvement in painful symptoms after the exercise protocol. For cannabinoid system there were an increase in CB2 expression in the ACC and PAG, and in CB1 levels in the PAG. And for opioid system there was an increase of MOR expression in the thalamus.
Thus, modulation of those receptors by physical exercise can be an important non-pharmacological intervention to reduce painful symptoms in a rat model of PD, contributing to knowledge and promotion of better treatment aimed at improving the quality of life of PD patients. |
| doi_str_mv | 10.1016/j.brainres.2019.146521 |
| format | Article |
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In addition to motor symptoms, Parkinson's disease (PD) presents high prevalence of painful symptoms responsible for worsening quality of life of PD patients. Physical exercise can improve such painful symptoms. This study evaluated the effects of exercise on nociceptive threshold using an unilateral rat model of PD, as well as the role played by cannabinoid and opioid receptors in areas responsible for pain pathways.
For PD induction, Wistar rats were injected with 6-OHDA. 15 days after, rats either remained sedentary or were forced to exercise three times a week for 40 min. Motor and nociceptive behaviors were evaluated through cylinder and mechanical hyperalgesia tests, respectively. The animals were euthanized for analysis of cannabinoid receptor type 1 (CB1) and type 2 (CB2), and μ-opioid receptor (MOR) in the anterior cingulate cortex (ACC), periaqueductal gray matter (PAG), and thalamus areas by immunohistochemistry (IHC) and Western blotting.
Our data revealed a decrease in the nociceptive threshold in both forepaws after surgery; in contrast, there was improvement in painful symptoms after the exercise protocol. For cannabinoid system there were an increase in CB2 expression in the ACC and PAG, and in CB1 levels in the PAG. And for opioid system there was an increase of MOR expression in the thalamus.
Thus, modulation of those receptors by physical exercise can be an important non-pharmacological intervention to reduce painful symptoms in a rat model of PD, contributing to knowledge and promotion of better treatment aimed at improving the quality of life of PD patients.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/j.brainres.2019.146521</identifier><identifier>PMID: 31697924</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>6-OHDA Parkinson’s disease model ; Analgesia ; Animals ; Cannabinoid receptors ; Disease Models, Animal ; Gyrus Cinguli - metabolism ; Hyperalgesia - complications ; Hyperalgesia - prevention & control ; Nociception - physiology ; Opioid receptors ; Painful symptoms ; Parkinson Disease - metabolism ; Parkinson Disease - prevention & control ; Parkinson Disease - psychology ; Periaqueductal Gray - metabolism ; Physical Conditioning, Animal ; Rats, Wistar ; Receptor, Cannabinoid, CB1 - metabolism ; Receptor, Cannabinoid, CB2 - metabolism ; Receptors, Opioid, mu - metabolism ; Treadmill exercise</subject><ispartof>Brain research, 2020-01, Vol.1727, p.146521-146521, Article 146521</ispartof><rights>2019 Elsevier B.V.</rights><rights>Copyright © 2019 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-41563bd22e16b52f7a7e3ed02c858654ee3c6efece6e1f3fa5c8cb60b61a898f3</citedby><cites>FETCH-LOGICAL-c368t-41563bd22e16b52f7a7e3ed02c858654ee3c6efece6e1f3fa5c8cb60b61a898f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S000689931930575X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31697924$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Binda, K.H.</creatorcontrib><creatorcontrib>Real, C.C.</creatorcontrib><creatorcontrib>Ferreira, A.F.F.</creatorcontrib><creatorcontrib>Britto, L.R.</creatorcontrib><creatorcontrib>Chacur, M.</creatorcontrib><title>Antinociceptive effects of treadmill exercise in a rat model of Parkinson's disease: The role of cannabinoid and opioid receptors</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>•Treadmill exercise promote antinociceptive effects in a rat model of Parkinson's disease.•Pain threshold improvement after treadmill exercise protocol in a 6-OHDA rat model of Parkinsońs disease.•Cannabinoids and opioids receptors role on antinociceptive effects in a rat model of Parkinsońs disease.
In addition to motor symptoms, Parkinson's disease (PD) presents high prevalence of painful symptoms responsible for worsening quality of life of PD patients. Physical exercise can improve such painful symptoms. This study evaluated the effects of exercise on nociceptive threshold using an unilateral rat model of PD, as well as the role played by cannabinoid and opioid receptors in areas responsible for pain pathways.
For PD induction, Wistar rats were injected with 6-OHDA. 15 days after, rats either remained sedentary or were forced to exercise three times a week for 40 min. Motor and nociceptive behaviors were evaluated through cylinder and mechanical hyperalgesia tests, respectively. The animals were euthanized for analysis of cannabinoid receptor type 1 (CB1) and type 2 (CB2), and μ-opioid receptor (MOR) in the anterior cingulate cortex (ACC), periaqueductal gray matter (PAG), and thalamus areas by immunohistochemistry (IHC) and Western blotting.
Our data revealed a decrease in the nociceptive threshold in both forepaws after surgery; in contrast, there was improvement in painful symptoms after the exercise protocol. For cannabinoid system there were an increase in CB2 expression in the ACC and PAG, and in CB1 levels in the PAG. And for opioid system there was an increase of MOR expression in the thalamus.
Thus, modulation of those receptors by physical exercise can be an important non-pharmacological intervention to reduce painful symptoms in a rat model of PD, contributing to knowledge and promotion of better treatment aimed at improving the quality of life of PD patients.</description><subject>6-OHDA Parkinson’s disease model</subject><subject>Analgesia</subject><subject>Animals</subject><subject>Cannabinoid receptors</subject><subject>Disease Models, Animal</subject><subject>Gyrus Cinguli - metabolism</subject><subject>Hyperalgesia - complications</subject><subject>Hyperalgesia - prevention & control</subject><subject>Nociception - physiology</subject><subject>Opioid receptors</subject><subject>Painful symptoms</subject><subject>Parkinson Disease - metabolism</subject><subject>Parkinson Disease - prevention & control</subject><subject>Parkinson Disease - psychology</subject><subject>Periaqueductal Gray - metabolism</subject><subject>Physical Conditioning, Animal</subject><subject>Rats, Wistar</subject><subject>Receptor, Cannabinoid, CB1 - metabolism</subject><subject>Receptor, Cannabinoid, CB2 - metabolism</subject><subject>Receptors, Opioid, mu - metabolism</subject><subject>Treadmill exercise</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1u3CAUhVHUKDNJ-woRu3bjCT82xl01GjU_0kjJIlkjDBeVqQ1T8ETJsm8erMl029XlSt_hwIfQJSUrSqi42q76pH1IkFeM0G5Fa9EweoKWVLasEqwmn9CSECIq2XV8gc5z3paV846coQWnoms7Vi_R3-sw-RCNN7Cb_AtgcA7MlHF0eEqg7eiHAcMrJOMzYB-wxklPeIwWhhl61Om3DzmGrxnbgugM3_HTL8ApDjADRoeg-9LhLdbB4rjz8zHB3BhT_oxOnR4yfPmYF-j55ufT-q7aPNzer683leFCTlVNG8F7yxhQ0TfMtboFDpYwIxspmhqAGwHl7SCAOu50Y6TpBekF1bKTjl-gb4d7dyn-2UOe1OizgWHQAeI-K8Yp541shSyoOKAmxZwTOLVLftTpTVGiZv1qq4761axfHfSX4OVHx74fwf6LHX0X4McBgPLTFw9JZeMhGLC-CJmUjf5_He-ipZxC</recordid><startdate>20200115</startdate><enddate>20200115</enddate><creator>Binda, K.H.</creator><creator>Real, C.C.</creator><creator>Ferreira, A.F.F.</creator><creator>Britto, L.R.</creator><creator>Chacur, M.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20200115</creationdate><title>Antinociceptive effects of treadmill exercise in a rat model of Parkinson's disease: The role of cannabinoid and opioid receptors</title><author>Binda, K.H. ; Real, C.C. ; Ferreira, A.F.F. ; Britto, L.R. ; Chacur, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-41563bd22e16b52f7a7e3ed02c858654ee3c6efece6e1f3fa5c8cb60b61a898f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>6-OHDA Parkinson’s disease model</topic><topic>Analgesia</topic><topic>Animals</topic><topic>Cannabinoid receptors</topic><topic>Disease Models, Animal</topic><topic>Gyrus Cinguli - metabolism</topic><topic>Hyperalgesia - complications</topic><topic>Hyperalgesia - prevention & control</topic><topic>Nociception - physiology</topic><topic>Opioid receptors</topic><topic>Painful symptoms</topic><topic>Parkinson Disease - metabolism</topic><topic>Parkinson Disease - prevention & control</topic><topic>Parkinson Disease - psychology</topic><topic>Periaqueductal Gray - metabolism</topic><topic>Physical Conditioning, Animal</topic><topic>Rats, Wistar</topic><topic>Receptor, Cannabinoid, CB1 - metabolism</topic><topic>Receptor, Cannabinoid, CB2 - metabolism</topic><topic>Receptors, Opioid, mu - metabolism</topic><topic>Treadmill exercise</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Binda, K.H.</creatorcontrib><creatorcontrib>Real, C.C.</creatorcontrib><creatorcontrib>Ferreira, A.F.F.</creatorcontrib><creatorcontrib>Britto, L.R.</creatorcontrib><creatorcontrib>Chacur, M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Binda, K.H.</au><au>Real, C.C.</au><au>Ferreira, A.F.F.</au><au>Britto, L.R.</au><au>Chacur, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antinociceptive effects of treadmill exercise in a rat model of Parkinson's disease: The role of cannabinoid and opioid receptors</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>2020-01-15</date><risdate>2020</risdate><volume>1727</volume><spage>146521</spage><epage>146521</epage><pages>146521-146521</pages><artnum>146521</artnum><issn>0006-8993</issn><eissn>1872-6240</eissn><abstract>•Treadmill exercise promote antinociceptive effects in a rat model of Parkinson's disease.•Pain threshold improvement after treadmill exercise protocol in a 6-OHDA rat model of Parkinsońs disease.•Cannabinoids and opioids receptors role on antinociceptive effects in a rat model of Parkinsońs disease.
In addition to motor symptoms, Parkinson's disease (PD) presents high prevalence of painful symptoms responsible for worsening quality of life of PD patients. Physical exercise can improve such painful symptoms. This study evaluated the effects of exercise on nociceptive threshold using an unilateral rat model of PD, as well as the role played by cannabinoid and opioid receptors in areas responsible for pain pathways.
For PD induction, Wistar rats were injected with 6-OHDA. 15 days after, rats either remained sedentary or were forced to exercise three times a week for 40 min. Motor and nociceptive behaviors were evaluated through cylinder and mechanical hyperalgesia tests, respectively. The animals were euthanized for analysis of cannabinoid receptor type 1 (CB1) and type 2 (CB2), and μ-opioid receptor (MOR) in the anterior cingulate cortex (ACC), periaqueductal gray matter (PAG), and thalamus areas by immunohistochemistry (IHC) and Western blotting.
Our data revealed a decrease in the nociceptive threshold in both forepaws after surgery; in contrast, there was improvement in painful symptoms after the exercise protocol. For cannabinoid system there were an increase in CB2 expression in the ACC and PAG, and in CB1 levels in the PAG. And for opioid system there was an increase of MOR expression in the thalamus.
Thus, modulation of those receptors by physical exercise can be an important non-pharmacological intervention to reduce painful symptoms in a rat model of PD, contributing to knowledge and promotion of better treatment aimed at improving the quality of life of PD patients.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>31697924</pmid><doi>10.1016/j.brainres.2019.146521</doi><tpages>1</tpages></addata></record> |
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| subjects | 6-OHDA Parkinson’s disease model Analgesia Animals Cannabinoid receptors Disease Models, Animal Gyrus Cinguli - metabolism Hyperalgesia - complications Hyperalgesia - prevention & control Nociception - physiology Opioid receptors Painful symptoms Parkinson Disease - metabolism Parkinson Disease - prevention & control Parkinson Disease - psychology Periaqueductal Gray - metabolism Physical Conditioning, Animal Rats, Wistar Receptor, Cannabinoid, CB1 - metabolism Receptor, Cannabinoid, CB2 - metabolism Receptors, Opioid, mu - metabolism Treadmill exercise |
| title | Antinociceptive effects of treadmill exercise in a rat model of Parkinson's disease: The role of cannabinoid and opioid receptors |
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