rM-CSF efficiently replaces L929 in generating mouse and rat bone marrow-derived macrophages for in vitro functional studies of immunity to intracellular bacteria

Methods used to prepare bone marrow-derived macrophages (BMDMs) may influence the outcomes of immunological assays in which they are used. Supernatant conditioned by growth of L929 cells has often been used to generate mouse macrophages from bone marrow in vitro but is subject to lot-to-lot variabil...

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Veröffentlicht in:	Journal of immunological methods 2020-02, Vol.477, p.112693-112693, Article 112693
Hauptverfasser:	Rice, Helen M., Rossi, Amy P., Bradford, Mary Katherine, Elkins, Karen L., De Pascalis, Roberto
Format:	Artikel
Sprache:	eng
Schlagworte:	Francisella tularensis in vitro testing L929 LVS Macrophage culture rM-CSF
Online-Zugang:	Volltext
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