Characterization and immunostimulatory activity of extracellular vesicles from Filifactor alocis
Filifactor alocis, a gram‐positive, obligate anaerobic rod, is an emerging periodontal pathogen that is frequently isolated from patients with periodontitis, peri‐implantitis, and apical periodontitis. Recent studies have shown that extracellular vesicles (EVs) from gram‐negative periodontal pathoge...
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| Veröffentlicht in: | Molecular oral microbiology 2020-01, Vol.35 (1), p.1-9 |
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| creator | Kim, Hyun Young Lim, Younggap An, Sun‐Jin Choi, Bong‐Kyu |
| description | Filifactor alocis, a gram‐positive, obligate anaerobic rod, is an emerging periodontal pathogen that is frequently isolated from patients with periodontitis, peri‐implantitis, and apical periodontitis. Recent studies have shown that extracellular vesicles (EVs) from gram‐negative periodontal pathogens, so‐called outer membrane vesicles (OMVs), harbor various effector molecules responsible for inducing host inflammatory responses. However, there are no reports of EVs from F. alocis. In this study, we purified and characterized the protein profiles of EVs from F. alocis and investigated their immunostimulatory activity on human monocytic THP‐1 and human oral keratinocyte HOK‐16B cell lines. Highly pure EVs were obtained from F. alocis using density gradient ultracentrifugation. Nanoparticle tracking analysis and transmission electron microscopy showed that F. alocis EVs were between 50 and 270 nm in diameter. Proteome analysis identified 28 proteins, including lipoproteins, autolysins, F. alocis complement inhibitor (FACIN), transporter‐related proteins, metabolism‐related proteins, and ribosomal proteins. Human cytokine array analysis showed that F. alocis EVs remarkably induced the expression of CCL1, CCL2, MIP‐1, CCL5, CXCL1, CXCL10, ICAM‐1, IL‐1β, IL‐1ra, IL‐6, IL‐8, MIF, SerpinE, and TNF‐α in THP‐1 cells and CXCL1, G‐CSF, GM‐CSF, IL‐6, and IL‐8 in HOK‐16B cells. The immunostimulatory activity of F. alocis EVs was similar to that of the whole bacterial cells. Our findings provide new insight into the role of EVs from gram‐positive oral bacteria in periodontal diseases.
F. alocis EVs harvor 28 proteins, including lipoproteins, FACIN, and autolysins. It remarkably induced pro‐inflammatory cytokines in human monocytes and human oral keratinocytes. |
| doi_str_mv | 10.1111/omi.12272 |
| format | Article |
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F. alocis EVs harvor 28 proteins, including lipoproteins, FACIN, and autolysins. It remarkably induced pro‐inflammatory cytokines in human monocytes and human oral keratinocytes.</description><identifier>ISSN: 2041-1006</identifier><identifier>EISSN: 2041-1014</identifier><identifier>DOI: 10.1111/omi.12272</identifier><identifier>PMID: 31675472</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>Autolysins ; Cell lines ; Chemokines ; Complement inhibitors ; CXCL10 protein ; cytokines ; Dentistry ; Extracellular vesicles ; gram‐positive bacteria ; Gum disease ; Immunostimulation ; Inflammation ; Lipoproteins ; Membrane vesicles ; Metabolism ; Monocyte chemoattractant protein 1 ; Monocytes ; Nanoparticles ; Pathogens ; Periodontal diseases ; Periodontitis ; Proteins ; Proteomes ; proteomics ; Ribosomal proteins ; Transmission electron microscopy ; Tumor necrosis factor ; Ultracentrifugation ; Vesicles</subject><ispartof>Molecular oral microbiology, 2020-01, Vol.35 (1), p.1-9</ispartof><rights>2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><rights>2019 John Wiley & Sons A/S</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3532-d81f2e52ed82088d409ce1e12b74ddbc6c87a65713bfb767c3d352f5349ed8613</citedby><cites>FETCH-LOGICAL-c3532-d81f2e52ed82088d409ce1e12b74ddbc6c87a65713bfb767c3d352f5349ed8613</cites><orcidid>0000-0003-3743-7209</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fomi.12272$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fomi.12272$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31675472$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Hyun Young</creatorcontrib><creatorcontrib>Lim, Younggap</creatorcontrib><creatorcontrib>An, Sun‐Jin</creatorcontrib><creatorcontrib>Choi, Bong‐Kyu</creatorcontrib><title>Characterization and immunostimulatory activity of extracellular vesicles from Filifactor alocis</title><title>Molecular oral microbiology</title><addtitle>Mol Oral Microbiol</addtitle><description>Filifactor alocis, a gram‐positive, obligate anaerobic rod, is an emerging periodontal pathogen that is frequently isolated from patients with periodontitis, peri‐implantitis, and apical periodontitis. Recent studies have shown that extracellular vesicles (EVs) from gram‐negative periodontal pathogens, so‐called outer membrane vesicles (OMVs), harbor various effector molecules responsible for inducing host inflammatory responses. However, there are no reports of EVs from F. alocis. In this study, we purified and characterized the protein profiles of EVs from F. alocis and investigated their immunostimulatory activity on human monocytic THP‐1 and human oral keratinocyte HOK‐16B cell lines. Highly pure EVs were obtained from F. alocis using density gradient ultracentrifugation. Nanoparticle tracking analysis and transmission electron microscopy showed that F. alocis EVs were between 50 and 270 nm in diameter. Proteome analysis identified 28 proteins, including lipoproteins, autolysins, F. alocis complement inhibitor (FACIN), transporter‐related proteins, metabolism‐related proteins, and ribosomal proteins. Human cytokine array analysis showed that F. alocis EVs remarkably induced the expression of CCL1, CCL2, MIP‐1, CCL5, CXCL1, CXCL10, ICAM‐1, IL‐1β, IL‐1ra, IL‐6, IL‐8, MIF, SerpinE, and TNF‐α in THP‐1 cells and CXCL1, G‐CSF, GM‐CSF, IL‐6, and IL‐8 in HOK‐16B cells. The immunostimulatory activity of F. alocis EVs was similar to that of the whole bacterial cells. Our findings provide new insight into the role of EVs from gram‐positive oral bacteria in periodontal diseases.
F. alocis EVs harvor 28 proteins, including lipoproteins, FACIN, and autolysins. It remarkably induced pro‐inflammatory cytokines in human monocytes and human oral keratinocytes.</description><subject>Autolysins</subject><subject>Cell lines</subject><subject>Chemokines</subject><subject>Complement inhibitors</subject><subject>CXCL10 protein</subject><subject>cytokines</subject><subject>Dentistry</subject><subject>Extracellular vesicles</subject><subject>gram‐positive bacteria</subject><subject>Gum disease</subject><subject>Immunostimulation</subject><subject>Inflammation</subject><subject>Lipoproteins</subject><subject>Membrane vesicles</subject><subject>Metabolism</subject><subject>Monocyte chemoattractant protein 1</subject><subject>Monocytes</subject><subject>Nanoparticles</subject><subject>Pathogens</subject><subject>Periodontal diseases</subject><subject>Periodontitis</subject><subject>Proteins</subject><subject>Proteomes</subject><subject>proteomics</subject><subject>Ribosomal proteins</subject><subject>Transmission electron microscopy</subject><subject>Tumor necrosis factor</subject><subject>Ultracentrifugation</subject><subject>Vesicles</subject><issn>2041-1006</issn><issn>2041-1014</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp10E9LwzAYBvAgipPpwS8gAS96qPbN3-4ow6kw2UXPNU1TjLSNJu20fnozqzsI5pJAfu_Dy4PQMaQXEM-la-wFECLJDjogKYMEUmC723cqJugohJc0HgpMSrmPJhSE5EySA_Q0f1Ze6c54-6k661qs2hLbpulbFzrb9LXqnB9wJHZtuwG7CpuPLo6Yuo6fHq9NsLo2AVfeNXhha1tF7DxWtdM2HKK9StXBHP3cU_S4uH6Y3ybL1c3d_GqZaMopScoMKmI4MWVG0iwrWTrTBgyQQrKyLLTQmVSCS6BFVUghNS0pJxWnbBZHBNApOhtzX717603o8saGzZKqNa4POaEAgnNGWaSnf-iL630bt4uKihkFLkVU56PS3oXgTZW_etsoP-SQ5pvm89h8_t18tCc_iX3RmHIrf3uO4HIE77Y2w_9J-er-boz8AvuujbU</recordid><startdate>202001</startdate><enddate>202001</enddate><creator>Kim, Hyun Young</creator><creator>Lim, Younggap</creator><creator>An, Sun‐Jin</creator><creator>Choi, Bong‐Kyu</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3743-7209</orcidid></search><sort><creationdate>202001</creationdate><title>Characterization and immunostimulatory activity of extracellular vesicles from Filifactor alocis</title><author>Kim, Hyun Young ; Lim, Younggap ; An, Sun‐Jin ; Choi, Bong‐Kyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3532-d81f2e52ed82088d409ce1e12b74ddbc6c87a65713bfb767c3d352f5349ed8613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Autolysins</topic><topic>Cell lines</topic><topic>Chemokines</topic><topic>Complement inhibitors</topic><topic>CXCL10 protein</topic><topic>cytokines</topic><topic>Dentistry</topic><topic>Extracellular vesicles</topic><topic>gram‐positive bacteria</topic><topic>Gum disease</topic><topic>Immunostimulation</topic><topic>Inflammation</topic><topic>Lipoproteins</topic><topic>Membrane vesicles</topic><topic>Metabolism</topic><topic>Monocyte chemoattractant protein 1</topic><topic>Monocytes</topic><topic>Nanoparticles</topic><topic>Pathogens</topic><topic>Periodontal diseases</topic><topic>Periodontitis</topic><topic>Proteins</topic><topic>Proteomes</topic><topic>proteomics</topic><topic>Ribosomal proteins</topic><topic>Transmission electron microscopy</topic><topic>Tumor necrosis factor</topic><topic>Ultracentrifugation</topic><topic>Vesicles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Hyun Young</creatorcontrib><creatorcontrib>Lim, Younggap</creatorcontrib><creatorcontrib>An, Sun‐Jin</creatorcontrib><creatorcontrib>Choi, Bong‐Kyu</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular oral microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Hyun Young</au><au>Lim, Younggap</au><au>An, Sun‐Jin</au><au>Choi, Bong‐Kyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization and immunostimulatory activity of extracellular vesicles from Filifactor alocis</atitle><jtitle>Molecular oral microbiology</jtitle><addtitle>Mol Oral Microbiol</addtitle><date>2020-01</date><risdate>2020</risdate><volume>35</volume><issue>1</issue><spage>1</spage><epage>9</epage><pages>1-9</pages><issn>2041-1006</issn><eissn>2041-1014</eissn><abstract>Filifactor alocis, a gram‐positive, obligate anaerobic rod, is an emerging periodontal pathogen that is frequently isolated from patients with periodontitis, peri‐implantitis, and apical periodontitis. Recent studies have shown that extracellular vesicles (EVs) from gram‐negative periodontal pathogens, so‐called outer membrane vesicles (OMVs), harbor various effector molecules responsible for inducing host inflammatory responses. However, there are no reports of EVs from F. alocis. In this study, we purified and characterized the protein profiles of EVs from F. alocis and investigated their immunostimulatory activity on human monocytic THP‐1 and human oral keratinocyte HOK‐16B cell lines. Highly pure EVs were obtained from F. alocis using density gradient ultracentrifugation. Nanoparticle tracking analysis and transmission electron microscopy showed that F. alocis EVs were between 50 and 270 nm in diameter. Proteome analysis identified 28 proteins, including lipoproteins, autolysins, F. alocis complement inhibitor (FACIN), transporter‐related proteins, metabolism‐related proteins, and ribosomal proteins. Human cytokine array analysis showed that F. alocis EVs remarkably induced the expression of CCL1, CCL2, MIP‐1, CCL5, CXCL1, CXCL10, ICAM‐1, IL‐1β, IL‐1ra, IL‐6, IL‐8, MIF, SerpinE, and TNF‐α in THP‐1 cells and CXCL1, G‐CSF, GM‐CSF, IL‐6, and IL‐8 in HOK‐16B cells. The immunostimulatory activity of F. alocis EVs was similar to that of the whole bacterial cells. Our findings provide new insight into the role of EVs from gram‐positive oral bacteria in periodontal diseases.
F. alocis EVs harvor 28 proteins, including lipoproteins, FACIN, and autolysins. It remarkably induced pro‐inflammatory cytokines in human monocytes and human oral keratinocytes.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31675472</pmid><doi>10.1111/omi.12272</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-3743-7209</orcidid></addata></record> |
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| subjects | Autolysins Cell lines Chemokines Complement inhibitors CXCL10 protein cytokines Dentistry Extracellular vesicles gram‐positive bacteria Gum disease Immunostimulation Inflammation Lipoproteins Membrane vesicles Metabolism Monocyte chemoattractant protein 1 Monocytes Nanoparticles Pathogens Periodontal diseases Periodontitis Proteins Proteomes proteomics Ribosomal proteins Transmission electron microscopy Tumor necrosis factor Ultracentrifugation Vesicles |
| title | Characterization and immunostimulatory activity of extracellular vesicles from Filifactor alocis |
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