Comparison of Real-Time Quantitative PCR and Digital Droplet PCR for BCR-ABL1 Monitoring in Patients with Chronic Myeloid Leukemia

Real-time quantitative PCR (qPCR) is routinely used to detect minimal residual disease in chronic myeloid leukemia patients. The absolute quantification with droplet digital PCR (ddPCR) could reduce the inherent variability of qPCR. We established a duplex ddPCR assay using the Europe against Cancer...

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Veröffentlicht in:	The Journal of molecular diagnostics : JMD 2020-01, Vol.22 (1), p.81-89
Hauptverfasser:	Franke, Georg-Nikolaus, Maier, Jacqueline, Wildenberger, Kathrin, Cross, Michael, Giles, Francis J., Müller, Martin C., Hochhaus, Andreas, Niederwieser, Dietger, Lange, Thoralf
Format:	Artikel
Sprache:	eng
Schlagworte:	Cohort Studies Feasibility Studies Fusion Proteins, bcr-abl - genetics HeLa Cells Humans K562 Cells Leukemia, Myelogenous, Chronic, BCR-ABL Positive - drug therapy Leukemia, Myelogenous, Chronic, BCR-ABL Positive - genetics Monitoring, Physiologic - methods Neoplasm, Residual - genetics Protein Kinase Inhibitors - therapeutic use Proto-Oncogene Proteins c-abl - genetics Real-Time Polymerase Chain Reaction - methods Sensitivity and Specificity
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