Identification of a Huperzine A-producing endophytic fungus from Phlegmariurus taxifolius
Highly prized huperzine A (Hup A), a natural alkaloid formerly isolated from the Chinese medicinal plant Huperzia serrata , has been widely used for the treatment of Alzheimer disease, inspiring us to search for endophytic fungi that produce this compound. In this study, we obtained the C17 fungus i...
Gespeichert in:
| Veröffentlicht in: | Molecular biology reports 2020, Vol.47 (1), p.489-495 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 495 |
|---|---|
| container_issue | 1 |
| container_start_page | 489 |
| container_title | Molecular biology reports |
| container_volume | 47 |
| creator | Cruz-Miranda, Olga Lidia Folch-Mallol, Jorge Martínez-Morales, Fernando Gesto-Borroto, Reinier Villarreal, María Luisa Taketa, Alexandre Cardoso |
| description | Highly prized huperzine A (Hup A), a natural alkaloid formerly isolated from the Chinese medicinal plant
Huperzia serrata
, has been widely used for the treatment of Alzheimer disease, inspiring us to search for endophytic fungi that produce this compound. In this study, we obtained the C17 fungus isolate from the Mexican club moss
Phlegmariurus taxifolius
, which produced a yield of 3.2 μg/g Hup A in mycelial dry weight, when cultured in potato dextrose broth medium. The C17 isolate was identified as belonging to the genus
Fusarium
with reference to the colony´s morphological characteristics and the presence of macroconidia and microconidia structures; and this was confirmed by DNA-barcoding analysis, by amplifying and sequencing the ribosomal internal transcribed spacer (rITS). |
| doi_str_mv | 10.1007/s11033-019-05155-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2310289504</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2330020135</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-7b11ac1d669504259dba4321306a14b468b2255a393a6c6c851585828edc114a3</originalsourceid><addsrcrecordid>eNp9kE1PGzEQhq2Kqglp_0AP1UpcuBhm7PV-HCPUAlIkOLSHniyv1xscbezUXkvAr8eQUCQOnCyNn3ln5iHkO8IZAtTnERE4p4AtBYFCUPxE5ihqTsu2bo7IHDggLRuBM3Ic4wYASqzFFzLjWIm2anFO_l73xk12sFpN1rvCD4UqrtLOhEfrTLGku-D7pK1bF8b1fnf3MFldDMmtUyyG4LfF7d1o1lsVbAq5NKl7O_jRpviVfB7UGM23w7sgf379_H1xRVc3l9cXyxXVvBYTrTtEpbGvqlZAyUTbd6rkDDlUCsuurJqOMSEUb7mqdKXzNaIRDWtMrxFLxRfkdJ-bN_2XTJzk1kZtxlE541OUjCOw5jk8oyfv0I1PweXtMsUBGCAXmWJ7SgcfYzCD3AWbD3yQCPJZvNyLl1m8fBEvMTf9OESnbmv6_y2vpjPA90DMX25twtvsD2KfAK5JjPo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2330020135</pqid></control><display><type>article</type><title>Identification of a Huperzine A-producing endophytic fungus from Phlegmariurus taxifolius</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Cruz-Miranda, Olga Lidia ; Folch-Mallol, Jorge ; Martínez-Morales, Fernando ; Gesto-Borroto, Reinier ; Villarreal, María Luisa ; Taketa, Alexandre Cardoso</creator><creatorcontrib>Cruz-Miranda, Olga Lidia ; Folch-Mallol, Jorge ; Martínez-Morales, Fernando ; Gesto-Borroto, Reinier ; Villarreal, María Luisa ; Taketa, Alexandre Cardoso</creatorcontrib><description>Highly prized huperzine A (Hup A), a natural alkaloid formerly isolated from the Chinese medicinal plant
Huperzia serrata
, has been widely used for the treatment of Alzheimer disease, inspiring us to search for endophytic fungi that produce this compound. In this study, we obtained the C17 fungus isolate from the Mexican club moss
Phlegmariurus taxifolius
, which produced a yield of 3.2 μg/g Hup A in mycelial dry weight, when cultured in potato dextrose broth medium. The C17 isolate was identified as belonging to the genus
Fusarium
with reference to the colony´s morphological characteristics and the presence of macroconidia and microconidia structures; and this was confirmed by DNA-barcoding analysis, by amplifying and sequencing the ribosomal internal transcribed spacer (rITS).</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-019-05155-1</identifier><identifier>PMID: 31659691</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Alkaloids - analysis ; Alkaloids - chemistry ; Alkaloids - isolation & purification ; Alzheimer's disease ; Animal Anatomy ; Animal Biochemistry ; Biomedical and Life Sciences ; Cholinesterase Inhibitors - analysis ; Cholinesterase Inhibitors - isolation & purification ; Cholinesterase Inhibitors - metabolism ; Dextrose ; DNA sequencing ; DNA, Fungal - genetics ; Endophytes ; Endophytes - chemistry ; Endophytes - isolation & purification ; Fungi ; Fusarium - chemistry ; Fusarium - classification ; Fusarium - genetics ; Fusarium - isolation & purification ; Histology ; Huperzine A ; Life Sciences ; Lycopodiaceae - microbiology ; Medicinal plants ; Morphology ; Mycelia ; Neurodegenerative diseases ; Original Article ; Physical characteristics ; Sesquiterpenes - analysis ; Sesquiterpenes - chemistry ; Sesquiterpenes - isolation & purification</subject><ispartof>Molecular biology reports, 2020, Vol.47 (1), p.489-495</ispartof><rights>Springer Nature B.V. 2019</rights><rights>Molecular Biology Reports is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-7b11ac1d669504259dba4321306a14b468b2255a393a6c6c851585828edc114a3</citedby><cites>FETCH-LOGICAL-c375t-7b11ac1d669504259dba4321306a14b468b2255a393a6c6c851585828edc114a3</cites><orcidid>0000-0001-7732-583X ; 0000-0001-9456-6651 ; 0000-0002-1860-8175 ; 0000-0003-0280-868X ; 0000-0003-3159-4203 ; 0000-0002-7055-0062</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-019-05155-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-019-05155-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51298</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31659691$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cruz-Miranda, Olga Lidia</creatorcontrib><creatorcontrib>Folch-Mallol, Jorge</creatorcontrib><creatorcontrib>Martínez-Morales, Fernando</creatorcontrib><creatorcontrib>Gesto-Borroto, Reinier</creatorcontrib><creatorcontrib>Villarreal, María Luisa</creatorcontrib><creatorcontrib>Taketa, Alexandre Cardoso</creatorcontrib><title>Identification of a Huperzine A-producing endophytic fungus from Phlegmariurus taxifolius</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Highly prized huperzine A (Hup A), a natural alkaloid formerly isolated from the Chinese medicinal plant
Huperzia serrata
, has been widely used for the treatment of Alzheimer disease, inspiring us to search for endophytic fungi that produce this compound. In this study, we obtained the C17 fungus isolate from the Mexican club moss
Phlegmariurus taxifolius
, which produced a yield of 3.2 μg/g Hup A in mycelial dry weight, when cultured in potato dextrose broth medium. The C17 isolate was identified as belonging to the genus
Fusarium
with reference to the colony´s morphological characteristics and the presence of macroconidia and microconidia structures; and this was confirmed by DNA-barcoding analysis, by amplifying and sequencing the ribosomal internal transcribed spacer (rITS).</description><subject>Alkaloids - analysis</subject><subject>Alkaloids - chemistry</subject><subject>Alkaloids - isolation & purification</subject><subject>Alzheimer's disease</subject><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cholinesterase Inhibitors - analysis</subject><subject>Cholinesterase Inhibitors - isolation & purification</subject><subject>Cholinesterase Inhibitors - metabolism</subject><subject>Dextrose</subject><subject>DNA sequencing</subject><subject>DNA, Fungal - genetics</subject><subject>Endophytes</subject><subject>Endophytes - chemistry</subject><subject>Endophytes - isolation & purification</subject><subject>Fungi</subject><subject>Fusarium - chemistry</subject><subject>Fusarium - classification</subject><subject>Fusarium - genetics</subject><subject>Fusarium - isolation & purification</subject><subject>Histology</subject><subject>Huperzine A</subject><subject>Life Sciences</subject><subject>Lycopodiaceae - microbiology</subject><subject>Medicinal plants</subject><subject>Morphology</subject><subject>Mycelia</subject><subject>Neurodegenerative diseases</subject><subject>Original Article</subject><subject>Physical characteristics</subject><subject>Sesquiterpenes - analysis</subject><subject>Sesquiterpenes - chemistry</subject><subject>Sesquiterpenes - isolation & purification</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kE1PGzEQhq2Kqglp_0AP1UpcuBhm7PV-HCPUAlIkOLSHniyv1xscbezUXkvAr8eQUCQOnCyNn3ln5iHkO8IZAtTnERE4p4AtBYFCUPxE5ihqTsu2bo7IHDggLRuBM3Ic4wYASqzFFzLjWIm2anFO_l73xk12sFpN1rvCD4UqrtLOhEfrTLGku-D7pK1bF8b1fnf3MFldDMmtUyyG4LfF7d1o1lsVbAq5NKl7O_jRpviVfB7UGM23w7sgf379_H1xRVc3l9cXyxXVvBYTrTtEpbGvqlZAyUTbd6rkDDlUCsuurJqOMSEUb7mqdKXzNaIRDWtMrxFLxRfkdJ-bN_2XTJzk1kZtxlE541OUjCOw5jk8oyfv0I1PweXtMsUBGCAXmWJ7SgcfYzCD3AWbD3yQCPJZvNyLl1m8fBEvMTf9OESnbmv6_y2vpjPA90DMX25twtvsD2KfAK5JjPo</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>Cruz-Miranda, Olga Lidia</creator><creator>Folch-Mallol, Jorge</creator><creator>Martínez-Morales, Fernando</creator><creator>Gesto-Borroto, Reinier</creator><creator>Villarreal, María Luisa</creator><creator>Taketa, Alexandre Cardoso</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7732-583X</orcidid><orcidid>https://orcid.org/0000-0001-9456-6651</orcidid><orcidid>https://orcid.org/0000-0002-1860-8175</orcidid><orcidid>https://orcid.org/0000-0003-0280-868X</orcidid><orcidid>https://orcid.org/0000-0003-3159-4203</orcidid><orcidid>https://orcid.org/0000-0002-7055-0062</orcidid></search><sort><creationdate>2020</creationdate><title>Identification of a Huperzine A-producing endophytic fungus from Phlegmariurus taxifolius</title><author>Cruz-Miranda, Olga Lidia ; Folch-Mallol, Jorge ; Martínez-Morales, Fernando ; Gesto-Borroto, Reinier ; Villarreal, María Luisa ; Taketa, Alexandre Cardoso</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-7b11ac1d669504259dba4321306a14b468b2255a393a6c6c851585828edc114a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Alkaloids - analysis</topic><topic>Alkaloids - chemistry</topic><topic>Alkaloids - isolation & purification</topic><topic>Alzheimer's disease</topic><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Cholinesterase Inhibitors - analysis</topic><topic>Cholinesterase Inhibitors - isolation & purification</topic><topic>Cholinesterase Inhibitors - metabolism</topic><topic>Dextrose</topic><topic>DNA sequencing</topic><topic>DNA, Fungal - genetics</topic><topic>Endophytes</topic><topic>Endophytes - chemistry</topic><topic>Endophytes - isolation & purification</topic><topic>Fungi</topic><topic>Fusarium - chemistry</topic><topic>Fusarium - classification</topic><topic>Fusarium - genetics</topic><topic>Fusarium - isolation & purification</topic><topic>Histology</topic><topic>Huperzine A</topic><topic>Life Sciences</topic><topic>Lycopodiaceae - microbiology</topic><topic>Medicinal plants</topic><topic>Morphology</topic><topic>Mycelia</topic><topic>Neurodegenerative diseases</topic><topic>Original Article</topic><topic>Physical characteristics</topic><topic>Sesquiterpenes - analysis</topic><topic>Sesquiterpenes - chemistry</topic><topic>Sesquiterpenes - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cruz-Miranda, Olga Lidia</creatorcontrib><creatorcontrib>Folch-Mallol, Jorge</creatorcontrib><creatorcontrib>Martínez-Morales, Fernando</creatorcontrib><creatorcontrib>Gesto-Borroto, Reinier</creatorcontrib><creatorcontrib>Villarreal, María Luisa</creatorcontrib><creatorcontrib>Taketa, Alexandre Cardoso</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cruz-Miranda, Olga Lidia</au><au>Folch-Mallol, Jorge</au><au>Martínez-Morales, Fernando</au><au>Gesto-Borroto, Reinier</au><au>Villarreal, María Luisa</au><au>Taketa, Alexandre Cardoso</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of a Huperzine A-producing endophytic fungus from Phlegmariurus taxifolius</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2020</date><risdate>2020</risdate><volume>47</volume><issue>1</issue><spage>489</spage><epage>495</epage><pages>489-495</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Highly prized huperzine A (Hup A), a natural alkaloid formerly isolated from the Chinese medicinal plant
Huperzia serrata
, has been widely used for the treatment of Alzheimer disease, inspiring us to search for endophytic fungi that produce this compound. In this study, we obtained the C17 fungus isolate from the Mexican club moss
Phlegmariurus taxifolius
, which produced a yield of 3.2 μg/g Hup A in mycelial dry weight, when cultured in potato dextrose broth medium. The C17 isolate was identified as belonging to the genus
Fusarium
with reference to the colony´s morphological characteristics and the presence of macroconidia and microconidia structures; and this was confirmed by DNA-barcoding analysis, by amplifying and sequencing the ribosomal internal transcribed spacer (rITS).</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>31659691</pmid><doi>10.1007/s11033-019-05155-1</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-7732-583X</orcidid><orcidid>https://orcid.org/0000-0001-9456-6651</orcidid><orcidid>https://orcid.org/0000-0002-1860-8175</orcidid><orcidid>https://orcid.org/0000-0003-0280-868X</orcidid><orcidid>https://orcid.org/0000-0003-3159-4203</orcidid><orcidid>https://orcid.org/0000-0002-7055-0062</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0301-4851 |
| ispartof | Molecular biology reports, 2020, Vol.47 (1), p.489-495 |
| issn | 0301-4851 1573-4978 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2310289504 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Alkaloids - analysis Alkaloids - chemistry Alkaloids - isolation & purification Alzheimer's disease Animal Anatomy Animal Biochemistry Biomedical and Life Sciences Cholinesterase Inhibitors - analysis Cholinesterase Inhibitors - isolation & purification Cholinesterase Inhibitors - metabolism Dextrose DNA sequencing DNA, Fungal - genetics Endophytes Endophytes - chemistry Endophytes - isolation & purification Fungi Fusarium - chemistry Fusarium - classification Fusarium - genetics Fusarium - isolation & purification Histology Huperzine A Life Sciences Lycopodiaceae - microbiology Medicinal plants Morphology Mycelia Neurodegenerative diseases Original Article Physical characteristics Sesquiterpenes - analysis Sesquiterpenes - chemistry Sesquiterpenes - isolation & purification |
| title | Identification of a Huperzine A-producing endophytic fungus from Phlegmariurus taxifolius |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T10%3A01%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20a%20Huperzine%20A-producing%20endophytic%20fungus%20from%20Phlegmariurus%20taxifolius&rft.jtitle=Molecular%20biology%20reports&rft.au=Cruz-Miranda,%20Olga%20Lidia&rft.date=2020&rft.volume=47&rft.issue=1&rft.spage=489&rft.epage=495&rft.pages=489-495&rft.issn=0301-4851&rft.eissn=1573-4978&rft_id=info:doi/10.1007/s11033-019-05155-1&rft_dat=%3Cproquest_cross%3E2330020135%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2330020135&rft_id=info:pmid/31659691&rfr_iscdi=true |