Novel multiplex strategy for DNA methylation-based age prediction from small amounts of DNA via Pyrosequencing

[Display omitted] •Multiplex amplification strategy was designed for combined quantitative methylation analysis of several CpG regions via Pyrosequencing.•Targets (including 15 age CpG sites): ELOVL2, FHL2, CCDC102B, C1orf132, KLF14, EDARADD, PDE4C and SST.•Both strategies performed equally precise...

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Veröffentlicht in:	Forensic science international : genetics 2020-01, Vol.44, p.102189-102189, Article 102189
Hauptverfasser:	Fleckhaus, Jan, Schneider, Peter M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aging - genetics Chronological age prediction CpG Islands - genetics DNA Methylation Female Forensic casework DNA samples Forensic Genetics - methods High-Throughput Nucleotide Sequencing - methods Humans Male Multiplex Polymerase Chain Reaction - methods Multiplex pyrosequencing Reproducibility of Results
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