Bioprocess optimization for purification of chimeric VLP displaying BVDV E2 antigens produced in yeast Hansenula polymorpha

•Antigens derived from BVDV E2 protein can be displayed on chimeric, dS-based VLP.•A DSP independent from ultracentrifugation yielded 93 ± 12 mg pure VLP per 100 g DCW.•The yield was improved ∼8fold compared to a previously applied, more complex DSP. Chimeric virus-like particles (VLP) are known as...

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