Time-dependent alteration to the tight junction structure of distal intestinal epithelia in type 2 prediabetic mice
High-fat diet (HFD) intake has been associated with changes in intestinal microbiota composition, increased intestinal permeability, and onset of type 2 diabetes mellitus (T2DM). The aim of this work was twofold: 1) to investigate the structural and functional alterations of the tight junction (TJ)-...
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| Veröffentlicht in: | Life sciences (1973) 2019-12, Vol.238, p.116971-116971, Article 116971 |
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| creator | de Oliveira, Ricardo Beltrame Matheus, Valquiria Aparecida Canuto, Leandro Pereira De Sant'ana, Ariane Collares-Buzato, Carla Beatriz |
| description | High-fat diet (HFD) intake has been associated with changes in intestinal microbiota composition, increased intestinal permeability, and onset of type 2 diabetes mellitus (T2DM). The aim of this work was twofold: 1) to investigate the structural and functional alterations of the tight junction (TJ)-mediated intestinal epithelial barrier of ileum and colon, that concentrate most of the microbiota, after exposure to a HFD for 15, 30 and 60 days, and 2) to assess the effect of in vitro exposure to free fatty acids (FFAs), one of the components of HFD, on paracellular barrier of colon-derived Caco-2 cells.
HFD exposure induced progressive metabolic changes in male mice that culminated in prediabetes after 60d. Morphological analysis of ileum and colon mucosa showed no signs of epithelial rupture or local inflammation but changes in the junctional content/distribution and/or cellular content of TJ-associated proteins (claudins-1, -2, -3, and occludin) in intestinal epithelia were seen mainly after a prediabetes state has been established. This impairment in TJ structure was not associated with significant changes in intestinal permeability to FITC-dextran. Exposure of Caco-2 monolayers to palmitic or linoleic acids seems to induce a reinforcement of TJ structure while treatment with oleic acid had a more diverse effect on TJ protein distribution.
TJ structure in distal intestinal epithelia can be specifically impaired by HFD intake at early stage of T2DM, but not by FFAs in vitro. Since the TJ change in ileum/colon was marginal, probably it does not contribute to the disease onset. |
| doi_str_mv | 10.1016/j.lfs.2019.116971 |
| format | Article |
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HFD exposure induced progressive metabolic changes in male mice that culminated in prediabetes after 60d. Morphological analysis of ileum and colon mucosa showed no signs of epithelial rupture or local inflammation but changes in the junctional content/distribution and/or cellular content of TJ-associated proteins (claudins-1, -2, -3, and occludin) in intestinal epithelia were seen mainly after a prediabetes state has been established. This impairment in TJ structure was not associated with significant changes in intestinal permeability to FITC-dextran. Exposure of Caco-2 monolayers to palmitic or linoleic acids seems to induce a reinforcement of TJ structure while treatment with oleic acid had a more diverse effect on TJ protein distribution.
TJ structure in distal intestinal epithelia can be specifically impaired by HFD intake at early stage of T2DM, but not by FFAs in vitro. Since the TJ change in ileum/colon was marginal, probably it does not contribute to the disease onset.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/j.lfs.2019.116971</identifier><identifier>PMID: 31634462</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Animals ; Caco-2 Cells ; Colon ; Dextran ; Diabetes mellitus ; Diabetes mellitus (non-insulin dependent) ; Diabetes Mellitus, Experimental - etiology ; Diabetes Mellitus, Experimental - metabolism ; Diabetes Mellitus, Experimental - pathology ; Diabetes Mellitus, Type 2 - etiology ; Diabetes Mellitus, Type 2 - metabolism ; Diabetes Mellitus, Type 2 - pathology ; Diet, High-Fat - adverse effects ; Exposure ; Fatty acids ; Free fatty acids ; High fat diet ; Humans ; Ileum ; Intestinal epithelial barrier ; Intestinal microflora ; Intestinal Mucosa - metabolism ; Intestinal Mucosa - pathology ; Intestine ; Male ; Mice ; Mice, Inbred C57BL ; Microbiota ; Mucosa ; Occludin ; Oleic acid ; Paracellular permeability ; Permeability ; Prediabetic State - etiology ; Prediabetic State - metabolism ; Prediabetic State - pathology ; Protein structure ; Proteins ; Structure-function relationships ; Tight junction ; Tight Junction Proteins - metabolism ; Tight Junctions - metabolism ; Tight Junctions - pathology ; Time dependence ; Time Factors ; Type 2 diabetes mellitus</subject><ispartof>Life sciences (1973), 2019-12, Vol.238, p.116971-116971, Article 116971</ispartof><rights>2019 Elsevier Inc.</rights><rights>Copyright © 2019 Elsevier Inc. All rights reserved.</rights><rights>Copyright Elsevier BV Dec 1, 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-68458086f082d3e1fe570c714f0db99e52739a3c5b6bef02bb4c35514b58bd043</citedby><cites>FETCH-LOGICAL-c381t-68458086f082d3e1fe570c714f0db99e52739a3c5b6bef02bb4c35514b58bd043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.lfs.2019.116971$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31634462$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Oliveira, Ricardo Beltrame</creatorcontrib><creatorcontrib>Matheus, Valquiria Aparecida</creatorcontrib><creatorcontrib>Canuto, Leandro Pereira</creatorcontrib><creatorcontrib>De Sant'ana, Ariane</creatorcontrib><creatorcontrib>Collares-Buzato, Carla Beatriz</creatorcontrib><title>Time-dependent alteration to the tight junction structure of distal intestinal epithelia in type 2 prediabetic mice</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>High-fat diet (HFD) intake has been associated with changes in intestinal microbiota composition, increased intestinal permeability, and onset of type 2 diabetes mellitus (T2DM). The aim of this work was twofold: 1) to investigate the structural and functional alterations of the tight junction (TJ)-mediated intestinal epithelial barrier of ileum and colon, that concentrate most of the microbiota, after exposure to a HFD for 15, 30 and 60 days, and 2) to assess the effect of in vitro exposure to free fatty acids (FFAs), one of the components of HFD, on paracellular barrier of colon-derived Caco-2 cells.
HFD exposure induced progressive metabolic changes in male mice that culminated in prediabetes after 60d. Morphological analysis of ileum and colon mucosa showed no signs of epithelial rupture or local inflammation but changes in the junctional content/distribution and/or cellular content of TJ-associated proteins (claudins-1, -2, -3, and occludin) in intestinal epithelia were seen mainly after a prediabetes state has been established. This impairment in TJ structure was not associated with significant changes in intestinal permeability to FITC-dextran. Exposure of Caco-2 monolayers to palmitic or linoleic acids seems to induce a reinforcement of TJ structure while treatment with oleic acid had a more diverse effect on TJ protein distribution.
TJ structure in distal intestinal epithelia can be specifically impaired by HFD intake at early stage of T2DM, but not by FFAs in vitro. Since the TJ change in ileum/colon was marginal, probably it does not contribute to the disease onset.</description><subject>Animals</subject><subject>Caco-2 Cells</subject><subject>Colon</subject><subject>Dextran</subject><subject>Diabetes mellitus</subject><subject>Diabetes mellitus (non-insulin dependent)</subject><subject>Diabetes Mellitus, Experimental - etiology</subject><subject>Diabetes Mellitus, Experimental - metabolism</subject><subject>Diabetes Mellitus, Experimental - pathology</subject><subject>Diabetes Mellitus, Type 2 - etiology</subject><subject>Diabetes Mellitus, Type 2 - metabolism</subject><subject>Diabetes Mellitus, Type 2 - pathology</subject><subject>Diet, High-Fat - adverse effects</subject><subject>Exposure</subject><subject>Fatty acids</subject><subject>Free fatty acids</subject><subject>High fat diet</subject><subject>Humans</subject><subject>Ileum</subject><subject>Intestinal epithelial barrier</subject><subject>Intestinal microflora</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intestinal Mucosa - pathology</subject><subject>Intestine</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Microbiota</subject><subject>Mucosa</subject><subject>Occludin</subject><subject>Oleic acid</subject><subject>Paracellular permeability</subject><subject>Permeability</subject><subject>Prediabetic State - etiology</subject><subject>Prediabetic State - metabolism</subject><subject>Prediabetic State - pathology</subject><subject>Protein structure</subject><subject>Proteins</subject><subject>Structure-function relationships</subject><subject>Tight junction</subject><subject>Tight Junction Proteins - metabolism</subject><subject>Tight Junctions - metabolism</subject><subject>Tight Junctions - pathology</subject><subject>Time dependence</subject><subject>Time Factors</subject><subject>Type 2 diabetes mellitus</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kTtvFTEQhS0EIjeBH0CDLNHQ7GX83F1RoQgIUiSaUFt-zBKv9oXtRcq_x5cbKCioxhp958x4DiGvGBwZMP1uPE5DPnJg_ZEx3bfsCTmwru0b0II9JQcALhvBQV2Qy5xHAFCqFc_JhWBaSKn5geS7OGMTcMMl4FKonQomW-K60LLSco-0xO_3hY774n93c0m7L3tCug40xFzsRONSMJe41CdusYqmaGuTlocNKadbwhCtwxI9naPHF-TZYKeMLx_rFfn26ePd9U1z-_Xzl-sPt40XHSuN7qTqoNMDdDwIZAOqFnzL5ADB9T0q3oreCq-cdjgAd056oRSTTnUugBRX5O3Zd0vrj71uaOaYPU6TXXDds-EC2pb3uu8q-uYfdFz3VD90ouocJnuhK8XOlE9rzgkHs6U42_RgGJhTImY0NRFzSsScE6ma14_Ou5sx_FX8iaAC788A1lP8jJhM9hEXX2-W0BcT1vgf-19Qi5wT</recordid><startdate>20191201</startdate><enddate>20191201</enddate><creator>de Oliveira, Ricardo Beltrame</creator><creator>Matheus, Valquiria Aparecida</creator><creator>Canuto, Leandro Pereira</creator><creator>De Sant'ana, Ariane</creator><creator>Collares-Buzato, Carla Beatriz</creator><general>Elsevier Inc</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20191201</creationdate><title>Time-dependent alteration to the tight junction structure of distal intestinal epithelia in type 2 prediabetic mice</title><author>de Oliveira, Ricardo Beltrame ; Matheus, Valquiria Aparecida ; Canuto, Leandro Pereira ; De Sant'ana, Ariane ; Collares-Buzato, Carla Beatriz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-68458086f082d3e1fe570c714f0db99e52739a3c5b6bef02bb4c35514b58bd043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Caco-2 Cells</topic><topic>Colon</topic><topic>Dextran</topic><topic>Diabetes mellitus</topic><topic>Diabetes mellitus (non-insulin dependent)</topic><topic>Diabetes Mellitus, Experimental - etiology</topic><topic>Diabetes Mellitus, Experimental - metabolism</topic><topic>Diabetes Mellitus, Experimental - pathology</topic><topic>Diabetes Mellitus, Type 2 - etiology</topic><topic>Diabetes Mellitus, Type 2 - metabolism</topic><topic>Diabetes Mellitus, Type 2 - pathology</topic><topic>Diet, High-Fat - adverse effects</topic><topic>Exposure</topic><topic>Fatty acids</topic><topic>Free fatty acids</topic><topic>High fat diet</topic><topic>Humans</topic><topic>Ileum</topic><topic>Intestinal epithelial barrier</topic><topic>Intestinal microflora</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Intestinal Mucosa - pathology</topic><topic>Intestine</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Microbiota</topic><topic>Mucosa</topic><topic>Occludin</topic><topic>Oleic acid</topic><topic>Paracellular permeability</topic><topic>Permeability</topic><topic>Prediabetic State - etiology</topic><topic>Prediabetic State - metabolism</topic><topic>Prediabetic State - pathology</topic><topic>Protein structure</topic><topic>Proteins</topic><topic>Structure-function relationships</topic><topic>Tight junction</topic><topic>Tight Junction Proteins - metabolism</topic><topic>Tight Junctions - metabolism</topic><topic>Tight Junctions - pathology</topic><topic>Time dependence</topic><topic>Time Factors</topic><topic>Type 2 diabetes mellitus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Oliveira, Ricardo Beltrame</creatorcontrib><creatorcontrib>Matheus, Valquiria Aparecida</creatorcontrib><creatorcontrib>Canuto, Leandro Pereira</creatorcontrib><creatorcontrib>De Sant'ana, Ariane</creatorcontrib><creatorcontrib>Collares-Buzato, Carla Beatriz</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Oliveira, Ricardo Beltrame</au><au>Matheus, Valquiria Aparecida</au><au>Canuto, Leandro Pereira</au><au>De Sant'ana, Ariane</au><au>Collares-Buzato, Carla Beatriz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Time-dependent alteration to the tight junction structure of distal intestinal epithelia in type 2 prediabetic mice</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2019-12-01</date><risdate>2019</risdate><volume>238</volume><spage>116971</spage><epage>116971</epage><pages>116971-116971</pages><artnum>116971</artnum><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>High-fat diet (HFD) intake has been associated with changes in intestinal microbiota composition, increased intestinal permeability, and onset of type 2 diabetes mellitus (T2DM). The aim of this work was twofold: 1) to investigate the structural and functional alterations of the tight junction (TJ)-mediated intestinal epithelial barrier of ileum and colon, that concentrate most of the microbiota, after exposure to a HFD for 15, 30 and 60 days, and 2) to assess the effect of in vitro exposure to free fatty acids (FFAs), one of the components of HFD, on paracellular barrier of colon-derived Caco-2 cells.
HFD exposure induced progressive metabolic changes in male mice that culminated in prediabetes after 60d. Morphological analysis of ileum and colon mucosa showed no signs of epithelial rupture or local inflammation but changes in the junctional content/distribution and/or cellular content of TJ-associated proteins (claudins-1, -2, -3, and occludin) in intestinal epithelia were seen mainly after a prediabetes state has been established. This impairment in TJ structure was not associated with significant changes in intestinal permeability to FITC-dextran. Exposure of Caco-2 monolayers to palmitic or linoleic acids seems to induce a reinforcement of TJ structure while treatment with oleic acid had a more diverse effect on TJ protein distribution.
TJ structure in distal intestinal epithelia can be specifically impaired by HFD intake at early stage of T2DM, but not by FFAs in vitro. Since the TJ change in ileum/colon was marginal, probably it does not contribute to the disease onset.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>31634462</pmid><doi>10.1016/j.lfs.2019.116971</doi><tpages>1</tpages></addata></record> |
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| subjects | Animals Caco-2 Cells Colon Dextran Diabetes mellitus Diabetes mellitus (non-insulin dependent) Diabetes Mellitus, Experimental - etiology Diabetes Mellitus, Experimental - metabolism Diabetes Mellitus, Experimental - pathology Diabetes Mellitus, Type 2 - etiology Diabetes Mellitus, Type 2 - metabolism Diabetes Mellitus, Type 2 - pathology Diet, High-Fat - adverse effects Exposure Fatty acids Free fatty acids High fat diet Humans Ileum Intestinal epithelial barrier Intestinal microflora Intestinal Mucosa - metabolism Intestinal Mucosa - pathology Intestine Male Mice Mice, Inbred C57BL Microbiota Mucosa Occludin Oleic acid Paracellular permeability Permeability Prediabetic State - etiology Prediabetic State - metabolism Prediabetic State - pathology Protein structure Proteins Structure-function relationships Tight junction Tight Junction Proteins - metabolism Tight Junctions - metabolism Tight Junctions - pathology Time dependence Time Factors Type 2 diabetes mellitus |
| title | Time-dependent alteration to the tight junction structure of distal intestinal epithelia in type 2 prediabetic mice |
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