Comparison of microplate- and bottle-based methods to age yeast for chronological life span assays
This study compared the chronological life span and survival of Saccharomyces cerevisiae aged in a microplate or bottle, under different aeration and calorie restriction conditions. Our data shows that limited aeration in the microplate-aged culture contributed to slower outgrowth but extended yeast...
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| Veröffentlicht in: | Journal of microbiological methods 2019-12, Vol.167, p.105743-105743, Article 105743 |
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| container_end_page | 105743 |
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| container_title | Journal of microbiological methods |
| container_volume | 167 |
| creator | Kwong, Mandy Mun Yee Lee, Jee Whu Samian, Mohammed Razip Watanabe, Nobumoto Osada, Hiroyuki Ong, Eugene Boon Beng |
| description | This study compared the chronological life span and survival of Saccharomyces cerevisiae aged in a microplate or bottle, under different aeration and calorie restriction conditions. Our data shows that limited aeration in the microplate-aged culture contributed to slower outgrowth but extended yeast CLS compared to the bottle-aged culture.
•Bottle- and microplate-ageing methods for the chronological life span assay of yeast Saccharomyces cerevisiae were compared.•Cultures were aged under different aeration and calorie restriction conditions.•Limited aeration of the culture in the microplate-ageing method causes slower outgrowth that leads to an extension of yeast CLS. |
| doi_str_mv | 10.1016/j.mimet.2019.105743 |
| format | Article |
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•Bottle- and microplate-ageing methods for the chronological life span assay of yeast Saccharomyces cerevisiae were compared.•Cultures were aged under different aeration and calorie restriction conditions.•Limited aeration of the culture in the microplate-ageing method causes slower outgrowth that leads to an extension of yeast CLS.</description><identifier>ISSN: 0167-7012</identifier><identifier>EISSN: 1872-8359</identifier><identifier>DOI: 10.1016/j.mimet.2019.105743</identifier><identifier>PMID: 31629019</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Aeration ; Calorie restriction ; Chronological life span ; Saccharomyces cerevisiae</subject><ispartof>Journal of microbiological methods, 2019-12, Vol.167, p.105743-105743, Article 105743</ispartof><rights>2019 Elsevier B.V.</rights><rights>Copyright © 2019 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-56cab3cc3b2f4616447a5d661b4c72167f8b94ae914916248cab3a5f537645763</citedby><cites>FETCH-LOGICAL-c425t-56cab3cc3b2f4616447a5d661b4c72167f8b94ae914916248cab3a5f537645763</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0167701219307213$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31629019$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kwong, Mandy Mun Yee</creatorcontrib><creatorcontrib>Lee, Jee Whu</creatorcontrib><creatorcontrib>Samian, Mohammed Razip</creatorcontrib><creatorcontrib>Watanabe, Nobumoto</creatorcontrib><creatorcontrib>Osada, Hiroyuki</creatorcontrib><creatorcontrib>Ong, Eugene Boon Beng</creatorcontrib><title>Comparison of microplate- and bottle-based methods to age yeast for chronological life span assays</title><title>Journal of microbiological methods</title><addtitle>J Microbiol Methods</addtitle><description>This study compared the chronological life span and survival of Saccharomyces cerevisiae aged in a microplate or bottle, under different aeration and calorie restriction conditions. Our data shows that limited aeration in the microplate-aged culture contributed to slower outgrowth but extended yeast CLS compared to the bottle-aged culture.
•Bottle- and microplate-ageing methods for the chronological life span assay of yeast Saccharomyces cerevisiae were compared.•Cultures were aged under different aeration and calorie restriction conditions.•Limited aeration of the culture in the microplate-ageing method causes slower outgrowth that leads to an extension of yeast CLS.</description><subject>Aeration</subject><subject>Calorie restriction</subject><subject>Chronological life span</subject><subject>Saccharomyces cerevisiae</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kDuP1DAUhS0EYoeFX4CEXNJkiN9JQYFGvKSVaKC2bpybXY-SOPh6kObf42EWSipL1nd8fD7GXot2L1ph3x33S1yw7GUr-npjnFZP2E50TjadMv1TtquUa1wr5A17QXRsW2GU7p6zGyWs7Gtsx4ZDWjbIkdLK08SXGHLaZijYcFhHPqRSZmwGIBx5LXtII_GSONwjPyNQ4VPKPDzktKY53ccAM5_jhJw2WDkQwZlesmcTzISvHs9b9uPTx--HL83dt89fDx_umqClKY2xAQYVghrkpK2wWjswo7Vi0MHJumTqhl4D9kL39fu6u-BgJqOc1cZZdcveXt_dcvp5Qip-iRRwnmHFdCIvVeuEcrKTFVVXtK4lyjj5LccF8tmL1l_k-qP_I9df5Pqr3Jp681hwGhYc_2X-2qzA-yuAdeaviNlTiLgGHGPGUPyY4n8LfgMOJYu5</recordid><startdate>201912</startdate><enddate>201912</enddate><creator>Kwong, Mandy Mun Yee</creator><creator>Lee, Jee Whu</creator><creator>Samian, Mohammed Razip</creator><creator>Watanabe, Nobumoto</creator><creator>Osada, Hiroyuki</creator><creator>Ong, Eugene Boon Beng</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201912</creationdate><title>Comparison of microplate- and bottle-based methods to age yeast for chronological life span assays</title><author>Kwong, Mandy Mun Yee ; Lee, Jee Whu ; Samian, Mohammed Razip ; Watanabe, Nobumoto ; Osada, Hiroyuki ; Ong, Eugene Boon Beng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-56cab3cc3b2f4616447a5d661b4c72167f8b94ae914916248cab3a5f537645763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Aeration</topic><topic>Calorie restriction</topic><topic>Chronological life span</topic><topic>Saccharomyces cerevisiae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kwong, Mandy Mun Yee</creatorcontrib><creatorcontrib>Lee, Jee Whu</creatorcontrib><creatorcontrib>Samian, Mohammed Razip</creatorcontrib><creatorcontrib>Watanabe, Nobumoto</creatorcontrib><creatorcontrib>Osada, Hiroyuki</creatorcontrib><creatorcontrib>Ong, Eugene Boon Beng</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kwong, Mandy Mun Yee</au><au>Lee, Jee Whu</au><au>Samian, Mohammed Razip</au><au>Watanabe, Nobumoto</au><au>Osada, Hiroyuki</au><au>Ong, Eugene Boon Beng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of microplate- and bottle-based methods to age yeast for chronological life span assays</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>2019-12</date><risdate>2019</risdate><volume>167</volume><spage>105743</spage><epage>105743</epage><pages>105743-105743</pages><artnum>105743</artnum><issn>0167-7012</issn><eissn>1872-8359</eissn><abstract>This study compared the chronological life span and survival of Saccharomyces cerevisiae aged in a microplate or bottle, under different aeration and calorie restriction conditions. Our data shows that limited aeration in the microplate-aged culture contributed to slower outgrowth but extended yeast CLS compared to the bottle-aged culture.
•Bottle- and microplate-ageing methods for the chronological life span assay of yeast Saccharomyces cerevisiae were compared.•Cultures were aged under different aeration and calorie restriction conditions.•Limited aeration of the culture in the microplate-ageing method causes slower outgrowth that leads to an extension of yeast CLS.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>31629019</pmid><doi>10.1016/j.mimet.2019.105743</doi><tpages>1</tpages></addata></record> |
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| issn | 0167-7012 1872-8359 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete |
| subjects | Aeration Calorie restriction Chronological life span Saccharomyces cerevisiae |
| title | Comparison of microplate- and bottle-based methods to age yeast for chronological life span assays |
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