Using fluorescence lifetime dequenching to estimate the average quinary stoichiometry of proteins in living cells

Biological proteins are understood in terms of five structural levels-primary, secondary, tertiary, quaternary and quinary. The quinary structure is defined as the set of macromolecular interactions that are transient in vivo. This includes non-covalent protein-protein interactions occurring within...

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Veröffentlicht in:	Methods and applications in fluorescence 2019-11, Vol.8 (1), p.014003-014003
Hauptverfasser:	Tran, Nguyen H N, Shagaghi, Nadin, Clayton, Andrew H A
Format:	Artikel
Sprache:	eng
Schlagworte:	FLIM Fluorescence HeLa Cells Humans Neoplasm Proteins - chemistry Protein Conformation protein-protein interaction quinary structure stoichiometry Time Factors Tumor Cells, Cultured
Online-Zugang:	Volltext
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