Ultrasensitive Fluorometric Angling Determination of Staphylococcus aureus in Vitro and Fluorescence Imaging in Vivo Using Carbon Dots with Full-Color Emission
Rapid, accurate, and safe screening of foodborne pathogenic bacteria is essential to effectively control and prevent outbreaks of foodborne illness. Fluorescent sensors constructed from carbon dots (CDs) and nanomaterial-based quenchers have provided an innovative method for screening of pathogenic...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2019-11, Vol.91 (22), p.14681-14690 |
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| creator | Cui, Fangchao Sun, Jiadi de Dieu Habimana, Jean Yang, Xingxing Ji, Jian Zhang, Yinzhi Lei, Hongtao Li, Zaijun Zheng, Jiayu Fan, Minghong Sun, Xiulan |
| description | Rapid, accurate, and safe screening of foodborne pathogenic bacteria is essential to effectively control and prevent outbreaks of foodborne illness. Fluorescent sensors constructed from carbon dots (CDs) and nanomaterial-based quenchers have provided an innovative method for screening of pathogenic bacteria. Herein, an ultrasensitive magnetic fluorescence aptasensor was designed for separation and detection of Staphylococcus aureus (S. aureus). Multicolor fluorescent CDs with a long fluorescent lifetime (6.73 ns) and high fluorescence stability were synthesized using a facile hydrothermal approach and modified cDNA as a highly sensitive fluorescent probe. CD fluorescence was quenched by Fe3O4 + aptamer via fluorescence resonance energy transfer (FRET). Under optimal conditions, the FRET-based aptasensor can detect S. aureus accompanied by a wide linear range of 50–107 CFU·mL–1 and a detection limit of 8 CFU·mL–1. Compared with other standard methods, this method was faster and more convenient, and the entire test was finished within 30 min. The capability of the aptasensor was simultaneously investigated on food samples. Additionally, the developed CDs exhibited excellent biocompatibility and were thus applied as fluorescent probes for bioimaging both in vitro and in vivo. This new platform provided an excellent application of the CDs for detecting and bioimaging pathogenic bacteria. |
| doi_str_mv | 10.1021/acs.analchem.9b03916 |
| format | Article |
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Fluorescent sensors constructed from carbon dots (CDs) and nanomaterial-based quenchers have provided an innovative method for screening of pathogenic bacteria. Herein, an ultrasensitive magnetic fluorescence aptasensor was designed for separation and detection of Staphylococcus aureus (S. aureus). Multicolor fluorescent CDs with a long fluorescent lifetime (6.73 ns) and high fluorescence stability were synthesized using a facile hydrothermal approach and modified cDNA as a highly sensitive fluorescent probe. CD fluorescence was quenched by Fe3O4 + aptamer via fluorescence resonance energy transfer (FRET). Under optimal conditions, the FRET-based aptasensor can detect S. aureus accompanied by a wide linear range of 50–107 CFU·mL–1 and a detection limit of 8 CFU·mL–1. Compared with other standard methods, this method was faster and more convenient, and the entire test was finished within 30 min. The capability of the aptasensor was simultaneously investigated on food samples. Additionally, the developed CDs exhibited excellent biocompatibility and were thus applied as fluorescent probes for bioimaging both in vitro and in vivo. 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Chem</addtitle><description>Rapid, accurate, and safe screening of foodborne pathogenic bacteria is essential to effectively control and prevent outbreaks of foodborne illness. Fluorescent sensors constructed from carbon dots (CDs) and nanomaterial-based quenchers have provided an innovative method for screening of pathogenic bacteria. Herein, an ultrasensitive magnetic fluorescence aptasensor was designed for separation and detection of Staphylococcus aureus (S. aureus). Multicolor fluorescent CDs with a long fluorescent lifetime (6.73 ns) and high fluorescence stability were synthesized using a facile hydrothermal approach and modified cDNA as a highly sensitive fluorescent probe. CD fluorescence was quenched by Fe3O4 + aptamer via fluorescence resonance energy transfer (FRET). Under optimal conditions, the FRET-based aptasensor can detect S. aureus accompanied by a wide linear range of 50–107 CFU·mL–1 and a detection limit of 8 CFU·mL–1. Compared with other standard methods, this method was faster and more convenient, and the entire test was finished within 30 min. The capability of the aptasensor was simultaneously investigated on food samples. Additionally, the developed CDs exhibited excellent biocompatibility and were thus applied as fluorescent probes for bioimaging both in vitro and in vivo. This new platform provided an excellent application of the CDs for detecting and bioimaging pathogenic bacteria.</description><subject>Analytical chemistry</subject><subject>Aptamers</subject><subject>Bacteria</subject><subject>Biocompatibility</subject><subject>Carbon dots</subject><subject>Chemistry</subject><subject>Energy transfer</subject><subject>Fluorescence</subject><subject>Fluorescence resonance energy transfer</subject><subject>Fluorescent indicators</subject><subject>Foodborne diseases</subject><subject>Foodborne pathogens</subject><subject>In vivo methods and tests</subject><subject>Iron oxides</subject><subject>Medical imaging</subject><subject>Nanomaterials</subject><subject>Screening</subject><subject>Staphylococcus aureus</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kcFu1DAURS0EokPhDxCyxIZNhmc7cZJlNe1ApUosaNlGjvMy48qxB9sp6tfwqzjMtAsWrJ4snXuvrEPIewZrBpx9VjqulVNW73Fatz2IlskXZMUqDoVsGv6SrABAFLwGOCNvYrwHYAyYfE3OBJOsFmW9Ir_vbAoqoosmmQekWzv74CdMwWh64XbWuB29xIRhMk4l4x31I_2e1GH_aL32Ws-RqjlgPsbRHyYFT5UbjkUYNTqN9HpSu6XoL_Hg6V1cXhsV-tx36VOkv0za0-1sbbHx1gd6NZkY89pb8mpUNuK70z0nt9ur283X4ubbl-vNxU2hRC1TIYSWMMpKMRSl0FzJshfQKDHKdvl0PUKFPR_bQSBTzVD1IEdALrAcSiHFOfl0rD0E_3PGmLq8r9Fa5dDPseMCJGct8AX9-A967-eQRSwUqzPCqyZT5ZHSwccYcOwOwUwqPHYMusVfl_11T_66k78c-3Aqn_sJh-fQk7AMwBFY4s_D_-38A9h0rFg</recordid><startdate>20191119</startdate><enddate>20191119</enddate><creator>Cui, Fangchao</creator><creator>Sun, Jiadi</creator><creator>de Dieu Habimana, Jean</creator><creator>Yang, Xingxing</creator><creator>Ji, Jian</creator><creator>Zhang, Yinzhi</creator><creator>Lei, Hongtao</creator><creator>Li, Zaijun</creator><creator>Zheng, Jiayu</creator><creator>Fan, Minghong</creator><creator>Sun, Xiulan</creator><general>American Chemical Society</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1528-8601</orcidid><orcidid>https://orcid.org/0000-0002-1697-1747</orcidid></search><sort><creationdate>20191119</creationdate><title>Ultrasensitive Fluorometric Angling Determination of Staphylococcus aureus in Vitro and Fluorescence Imaging in Vivo Using Carbon Dots with Full-Color Emission</title><author>Cui, Fangchao ; Sun, Jiadi ; de Dieu Habimana, Jean ; Yang, Xingxing ; Ji, Jian ; Zhang, Yinzhi ; Lei, Hongtao ; Li, Zaijun ; Zheng, Jiayu ; Fan, Minghong ; Sun, Xiulan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a376t-33c60f65a1e343c2a64b308a3f6911017f05eb2f9d3e1a8d5b06f0e23e4d4363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Analytical chemistry</topic><topic>Aptamers</topic><topic>Bacteria</topic><topic>Biocompatibility</topic><topic>Carbon dots</topic><topic>Chemistry</topic><topic>Energy transfer</topic><topic>Fluorescence</topic><topic>Fluorescence resonance energy transfer</topic><topic>Fluorescent indicators</topic><topic>Foodborne diseases</topic><topic>Foodborne pathogens</topic><topic>In vivo methods and tests</topic><topic>Iron oxides</topic><topic>Medical imaging</topic><topic>Nanomaterials</topic><topic>Screening</topic><topic>Staphylococcus aureus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cui, Fangchao</creatorcontrib><creatorcontrib>Sun, Jiadi</creatorcontrib><creatorcontrib>de Dieu Habimana, Jean</creatorcontrib><creatorcontrib>Yang, Xingxing</creatorcontrib><creatorcontrib>Ji, Jian</creatorcontrib><creatorcontrib>Zhang, Yinzhi</creatorcontrib><creatorcontrib>Lei, Hongtao</creatorcontrib><creatorcontrib>Li, Zaijun</creatorcontrib><creatorcontrib>Zheng, Jiayu</creatorcontrib><creatorcontrib>Fan, Minghong</creatorcontrib><creatorcontrib>Sun, Xiulan</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cui, Fangchao</au><au>Sun, Jiadi</au><au>de Dieu Habimana, Jean</au><au>Yang, Xingxing</au><au>Ji, Jian</au><au>Zhang, Yinzhi</au><au>Lei, Hongtao</au><au>Li, Zaijun</au><au>Zheng, Jiayu</au><au>Fan, Minghong</au><au>Sun, Xiulan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrasensitive Fluorometric Angling Determination of Staphylococcus aureus in Vitro and Fluorescence Imaging in Vivo Using Carbon Dots with Full-Color Emission</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2019-11-19</date><risdate>2019</risdate><volume>91</volume><issue>22</issue><spage>14681</spage><epage>14690</epage><pages>14681-14690</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><abstract>Rapid, accurate, and safe screening of foodborne pathogenic bacteria is essential to effectively control and prevent outbreaks of foodborne illness. Fluorescent sensors constructed from carbon dots (CDs) and nanomaterial-based quenchers have provided an innovative method for screening of pathogenic bacteria. Herein, an ultrasensitive magnetic fluorescence aptasensor was designed for separation and detection of Staphylococcus aureus (S. aureus). Multicolor fluorescent CDs with a long fluorescent lifetime (6.73 ns) and high fluorescence stability were synthesized using a facile hydrothermal approach and modified cDNA as a highly sensitive fluorescent probe. CD fluorescence was quenched by Fe3O4 + aptamer via fluorescence resonance energy transfer (FRET). Under optimal conditions, the FRET-based aptasensor can detect S. aureus accompanied by a wide linear range of 50–107 CFU·mL–1 and a detection limit of 8 CFU·mL–1. Compared with other standard methods, this method was faster and more convenient, and the entire test was finished within 30 min. The capability of the aptasensor was simultaneously investigated on food samples. Additionally, the developed CDs exhibited excellent biocompatibility and were thus applied as fluorescent probes for bioimaging both in vitro and in vivo. This new platform provided an excellent application of the CDs for detecting and bioimaging pathogenic bacteria.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>31617347</pmid><doi>10.1021/acs.analchem.9b03916</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-1528-8601</orcidid><orcidid>https://orcid.org/0000-0002-1697-1747</orcidid></addata></record> |
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| ispartof | Analytical chemistry (Washington), 2019-11, Vol.91 (22), p.14681-14690 |
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| subjects | Analytical chemistry Aptamers Bacteria Biocompatibility Carbon dots Chemistry Energy transfer Fluorescence Fluorescence resonance energy transfer Fluorescent indicators Foodborne diseases Foodborne pathogens In vivo methods and tests Iron oxides Medical imaging Nanomaterials Screening Staphylococcus aureus |
| title | Ultrasensitive Fluorometric Angling Determination of Staphylococcus aureus in Vitro and Fluorescence Imaging in Vivo Using Carbon Dots with Full-Color Emission |
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