Immobilization of Lactobacillus rhamnosus in polyvinyl alcohol/calcium alginate matrix for production of lactic acid
Immobilization of Lactobacillus rhamnosus ATCC7469 in poly(vinyl alcohol)/calcium alginate (PVA/Ca-alginate) matrix using “freezing–thawing” technique for application in lactic acid (LA) fermentation was studied in this paper. PVA/Ca-alginate beads were made from sterile and non-sterile PVA and sodi...
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| Veröffentlicht in: | Bioprocess and biosystems engineering 2020-02, Vol.43 (2), p.315-322 |
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| creator | Radosavljević, Miloš Lević, Steva Belović, Miona Pejin, Jelena Djukić-Vuković, Aleksandra Mojović, Ljiljana Nedović, Viktor |
| description | Immobilization of
Lactobacillus rhamnosus
ATCC7469 in poly(vinyl alcohol)/calcium alginate (PVA/Ca-alginate) matrix using “freezing–thawing” technique for application in lactic acid (LA) fermentation was studied in this paper. PVA/Ca-alginate beads were made from sterile and non-sterile PVA and sodium alginate solutions. According to mechanical properties, the PVA/Ca-alginate beads expressed a strong elastic character. Obtained PVA/Ca-alginate beads were further applied in batch and repeated batch LA fermentations. Regarding cell viability,
L. rhamnosus
cells survived well rather sharp immobilization procedure and significant cell proliferation was observed in further fermentation studies achieving high cell viability (up to 10.7 log CFU g
−1
) in sterile beads. In batch LA fermentation, the immobilized biocatalyst was superior to free cell fermentation system (by 37.1%), while the highest LA yield and volumetric productivity of 97.6% and 0.8 g L
−1
h
−1
, respectively, were attained in repeated batch fermentation. During seven consecutive batch fermentations, the biocatalyst showed high mechanical and operational stability reaching an overall productivity of 0.78 g L
−1
h
−1
. This study suggested that the “freezing–thawing” technique can be successfully used for immobilization of
L. rhamnosus
in PVA/Ca-alginate matrix without loss of either viability or LA fermentation capability. |
| doi_str_mv | 10.1007/s00449-019-02228-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2305030583</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2342423875</sourcerecordid><originalsourceid>FETCH-LOGICAL-c412t-89d270ac37b207842d38044059d12470f64bba223a0e4509f3911367907f42e93</originalsourceid><addsrcrecordid>eNp9kUFv1DAQhS0EoqXwBzggS1x6CR2P7XVyRFULlVbiUs6W4zitKyde7ASx_HqmbBckDhxGfpa_eTPWY-ytgA8CwFxUAKW6BgQVIrYNPGOnYiN0Yzagnx-17sQJe1XrA4DQLcJLdiIFAQj6lC0305T7mOJPt8Q88zzyrfNL7p2PKa2Vl3s3zbmSijPf5bT_Hud94i75fJ_ThScR14nud3F2S-CTW0r8wcdc-K7kYfVH20S20XPyHV6zF6NLNbx5Os_Y1-ur28vPzfbLp5vLj9vGK4FL03YDGnBemh7BtAoH2dKPQXeDQGVg3Ki-d4jSQVAaulF2QsiN6cCMCkMnz9j5wZc2-baGutgpVh9ScnPIa7UoQQNVKwl9_w_6kNcy03ZEKVQoW6OJwgPlS661hNHuSpxc2VsB9jETe8jEUib2dyYWqOndk_XaT2H403IMgQB5ACo9zXeh_J39H9tfCzmWvA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2342423875</pqid></control><display><type>article</type><title>Immobilization of Lactobacillus rhamnosus in polyvinyl alcohol/calcium alginate matrix for production of lactic acid</title><source>SpringerLink Journals - AutoHoldings</source><creator>Radosavljević, Miloš ; Lević, Steva ; Belović, Miona ; Pejin, Jelena ; Djukić-Vuković, Aleksandra ; Mojović, Ljiljana ; Nedović, Viktor</creator><creatorcontrib>Radosavljević, Miloš ; Lević, Steva ; Belović, Miona ; Pejin, Jelena ; Djukić-Vuković, Aleksandra ; Mojović, Ljiljana ; Nedović, Viktor</creatorcontrib><description>Immobilization of
Lactobacillus rhamnosus
ATCC7469 in poly(vinyl alcohol)/calcium alginate (PVA/Ca-alginate) matrix using “freezing–thawing” technique for application in lactic acid (LA) fermentation was studied in this paper. PVA/Ca-alginate beads were made from sterile and non-sterile PVA and sodium alginate solutions. According to mechanical properties, the PVA/Ca-alginate beads expressed a strong elastic character. Obtained PVA/Ca-alginate beads were further applied in batch and repeated batch LA fermentations. Regarding cell viability,
L. rhamnosus
cells survived well rather sharp immobilization procedure and significant cell proliferation was observed in further fermentation studies achieving high cell viability (up to 10.7 log CFU g
−1
) in sterile beads. In batch LA fermentation, the immobilized biocatalyst was superior to free cell fermentation system (by 37.1%), while the highest LA yield and volumetric productivity of 97.6% and 0.8 g L
−1
h
−1
, respectively, were attained in repeated batch fermentation. During seven consecutive batch fermentations, the biocatalyst showed high mechanical and operational stability reaching an overall productivity of 0.78 g L
−1
h
−1
. This study suggested that the “freezing–thawing” technique can be successfully used for immobilization of
L. rhamnosus
in PVA/Ca-alginate matrix without loss of either viability or LA fermentation capability.</description><identifier>ISSN: 1615-7591</identifier><identifier>EISSN: 1615-7605</identifier><identifier>DOI: 10.1007/s00449-019-02228-0</identifier><identifier>PMID: 31605205</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Alginic acid ; Beads ; Biocatalysts ; Biotechnology ; Calcium ; Calcium alginate ; Cell proliferation ; Cell viability ; Chemistry ; Chemistry and Materials Science ; Environmental Engineering/Biotechnology ; Fermentation ; Food Science ; Freezing ; Immobilization ; Industrial and Production Engineering ; Industrial Chemistry/Chemical Engineering ; Lactic acid ; Lactobacilli ; Lactobacillus rhamnosus ; Mechanical properties ; Melting ; Polyvinyl alcohol ; Productivity ; Research Paper ; Sodium alginate ; Thawing</subject><ispartof>Bioprocess and biosystems engineering, 2020-02, Vol.43 (2), p.315-322</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2019</rights><rights>Bioprocess and Biosystems Engineering is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-89d270ac37b207842d38044059d12470f64bba223a0e4509f3911367907f42e93</citedby><cites>FETCH-LOGICAL-c412t-89d270ac37b207842d38044059d12470f64bba223a0e4509f3911367907f42e93</cites><orcidid>0000-0001-6473-5282</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00449-019-02228-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00449-019-02228-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31605205$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Radosavljević, Miloš</creatorcontrib><creatorcontrib>Lević, Steva</creatorcontrib><creatorcontrib>Belović, Miona</creatorcontrib><creatorcontrib>Pejin, Jelena</creatorcontrib><creatorcontrib>Djukić-Vuković, Aleksandra</creatorcontrib><creatorcontrib>Mojović, Ljiljana</creatorcontrib><creatorcontrib>Nedović, Viktor</creatorcontrib><title>Immobilization of Lactobacillus rhamnosus in polyvinyl alcohol/calcium alginate matrix for production of lactic acid</title><title>Bioprocess and biosystems engineering</title><addtitle>Bioprocess Biosyst Eng</addtitle><addtitle>Bioprocess Biosyst Eng</addtitle><description>Immobilization of
Lactobacillus rhamnosus
ATCC7469 in poly(vinyl alcohol)/calcium alginate (PVA/Ca-alginate) matrix using “freezing–thawing” technique for application in lactic acid (LA) fermentation was studied in this paper. PVA/Ca-alginate beads were made from sterile and non-sterile PVA and sodium alginate solutions. According to mechanical properties, the PVA/Ca-alginate beads expressed a strong elastic character. Obtained PVA/Ca-alginate beads were further applied in batch and repeated batch LA fermentations. Regarding cell viability,
L. rhamnosus
cells survived well rather sharp immobilization procedure and significant cell proliferation was observed in further fermentation studies achieving high cell viability (up to 10.7 log CFU g
−1
) in sterile beads. In batch LA fermentation, the immobilized biocatalyst was superior to free cell fermentation system (by 37.1%), while the highest LA yield and volumetric productivity of 97.6% and 0.8 g L
−1
h
−1
, respectively, were attained in repeated batch fermentation. During seven consecutive batch fermentations, the biocatalyst showed high mechanical and operational stability reaching an overall productivity of 0.78 g L
−1
h
−1
. This study suggested that the “freezing–thawing” technique can be successfully used for immobilization of
L. rhamnosus
in PVA/Ca-alginate matrix without loss of either viability or LA fermentation capability.</description><subject>Alginic acid</subject><subject>Beads</subject><subject>Biocatalysts</subject><subject>Biotechnology</subject><subject>Calcium</subject><subject>Calcium alginate</subject><subject>Cell proliferation</subject><subject>Cell viability</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Fermentation</subject><subject>Food Science</subject><subject>Freezing</subject><subject>Immobilization</subject><subject>Industrial and Production Engineering</subject><subject>Industrial Chemistry/Chemical Engineering</subject><subject>Lactic acid</subject><subject>Lactobacilli</subject><subject>Lactobacillus rhamnosus</subject><subject>Mechanical properties</subject><subject>Melting</subject><subject>Polyvinyl alcohol</subject><subject>Productivity</subject><subject>Research 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Production Engineering</topic><topic>Industrial Chemistry/Chemical Engineering</topic><topic>Lactic acid</topic><topic>Lactobacilli</topic><topic>Lactobacillus rhamnosus</topic><topic>Mechanical properties</topic><topic>Melting</topic><topic>Polyvinyl alcohol</topic><topic>Productivity</topic><topic>Research Paper</topic><topic>Sodium alginate</topic><topic>Thawing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Radosavljević, Miloš</creatorcontrib><creatorcontrib>Lević, Steva</creatorcontrib><creatorcontrib>Belović, Miona</creatorcontrib><creatorcontrib>Pejin, Jelena</creatorcontrib><creatorcontrib>Djukić-Vuković, Aleksandra</creatorcontrib><creatorcontrib>Mojović, Ljiljana</creatorcontrib><creatorcontrib>Nedović, Viktor</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial 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engineering</jtitle><stitle>Bioprocess Biosyst Eng</stitle><addtitle>Bioprocess Biosyst Eng</addtitle><date>2020-02-01</date><risdate>2020</risdate><volume>43</volume><issue>2</issue><spage>315</spage><epage>322</epage><pages>315-322</pages><issn>1615-7591</issn><eissn>1615-7605</eissn><abstract>Immobilization of
Lactobacillus rhamnosus
ATCC7469 in poly(vinyl alcohol)/calcium alginate (PVA/Ca-alginate) matrix using “freezing–thawing” technique for application in lactic acid (LA) fermentation was studied in this paper. PVA/Ca-alginate beads were made from sterile and non-sterile PVA and sodium alginate solutions. According to mechanical properties, the PVA/Ca-alginate beads expressed a strong elastic character. Obtained PVA/Ca-alginate beads were further applied in batch and repeated batch LA fermentations. Regarding cell viability,
L. rhamnosus
cells survived well rather sharp immobilization procedure and significant cell proliferation was observed in further fermentation studies achieving high cell viability (up to 10.7 log CFU g
−1
) in sterile beads. In batch LA fermentation, the immobilized biocatalyst was superior to free cell fermentation system (by 37.1%), while the highest LA yield and volumetric productivity of 97.6% and 0.8 g L
−1
h
−1
, respectively, were attained in repeated batch fermentation. During seven consecutive batch fermentations, the biocatalyst showed high mechanical and operational stability reaching an overall productivity of 0.78 g L
−1
h
−1
. This study suggested that the “freezing–thawing” technique can be successfully used for immobilization of
L. rhamnosus
in PVA/Ca-alginate matrix without loss of either viability or LA fermentation capability.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>31605205</pmid><doi>10.1007/s00449-019-02228-0</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-6473-5282</orcidid></addata></record> |
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| subjects | Alginic acid Beads Biocatalysts Biotechnology Calcium Calcium alginate Cell proliferation Cell viability Chemistry Chemistry and Materials Science Environmental Engineering/Biotechnology Fermentation Food Science Freezing Immobilization Industrial and Production Engineering Industrial Chemistry/Chemical Engineering Lactic acid Lactobacilli Lactobacillus rhamnosus Mechanical properties Melting Polyvinyl alcohol Productivity Research Paper Sodium alginate Thawing |
| title | Immobilization of Lactobacillus rhamnosus in polyvinyl alcohol/calcium alginate matrix for production of lactic acid |
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