A Novel Nicotinamide Adenine Dinucleotide Correction Method for Intracellular Ca2+ Measurement with Fura-2-Analog in Live Cells

To measure [Ca2+] quantitatively, fura-2 analogs, which are ratiometric fluoroprobes, are frequently used. However, dye usage is intrinsically limited in live cells because of autofluorescence interference, mainly from nicotinamide adenine dinucleotide (NADH). More specifically, this is a major obst...

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Veröffentlicht in:Journal of Visualized Experiments 2019-09 (151)
Hauptverfasser: Lee, Jeong Hoon, Ha, Jeong Mi, Ho, Quynh Mai, Leem, Chae Hun
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Sprache:eng
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