Morphological difference between pleural mesothelioma cells in effusion smears with either BAP1 loss or 9p21 homozygous deletion and reactive mesothelial cells without the gene alterations
We previously characterized the morphological characteristics of malignant pleural mesothelioma (MPM) cells with 9p21 homozygous deletion (HD) using a combination of the virtual microscopy and fluorescence in situ hybridization (FISH). In this study, we investigated whether MPM cells with BRCA1‐asso...
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| Veröffentlicht in: | Pathology international 2019-11, Vol.69 (11), p.637-645 |
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| creator | Matsumoto, Shinji Hamasaki, Makoto Kinoshita, Yoshiaki Kamei, Toshiaki Kawahara, Kunimitsu Nabeshima, Kazuki |
| description | We previously characterized the morphological characteristics of malignant pleural mesothelioma (MPM) cells with 9p21 homozygous deletion (HD) using a combination of the virtual microscopy and fluorescence in situ hybridization (FISH). In this study, we investigated whether MPM cells with BRCA1‐associated protein 1 (BAP1) loss show the same morphological characteristics identified in MPM cells with 9p21 HD. MPM cells with either BAP1 loss detected by immunocytochemistry (ICC) or 9p21 HD detected by FISH were identified via virtual microscopy prior to ICC or FISH, followed by analysis and quantification of their morphological characteristics. MPM cells with BAP1 loss or 9p21 HD exhibited significantly more frequent cell‐in‐cell engulfment, multinucleation, and larger multicellular clusters composed of more than 10 cells than reactive mesothelial cells. In conclusion, MPM cells with BAP1 loss or 9p21 HD share similar cytological features, indicating that the same morphological criteria can be used to detect MPM cells harboring such genetic aberrations. |
| doi_str_mv | 10.1111/pin.12862 |
| format | Article |
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In this study, we investigated whether MPM cells with BRCA1‐associated protein 1 (BAP1) loss show the same morphological characteristics identified in MPM cells with 9p21 HD. MPM cells with either BAP1 loss detected by immunocytochemistry (ICC) or 9p21 HD detected by FISH were identified via virtual microscopy prior to ICC or FISH, followed by analysis and quantification of their morphological characteristics. MPM cells with BAP1 loss or 9p21 HD exhibited significantly more frequent cell‐in‐cell engulfment, multinucleation, and larger multicellular clusters composed of more than 10 cells than reactive mesothelial cells. In conclusion, MPM cells with BAP1 loss or 9p21 HD share similar cytological features, indicating that the same morphological criteria can be used to detect MPM cells harboring such genetic aberrations.</description><identifier>ISSN: 1320-5463</identifier><identifier>EISSN: 1440-1827</identifier><identifier>DOI: 10.1111/pin.12862</identifier><identifier>PMID: 31580004</identifier><language>eng</language><publisher>Australia: Wiley Subscription Services, Inc</publisher><subject>9p21 homozygous deletion ; BAP1 loss ; BRCA1 protein ; Breast cancer ; Clonal deletion ; Effusion ; effusion smear ; Fluorescence ; Fluorescence in situ hybridization ; Gene deletion ; Immunocytochemistry ; Mesothelioma ; Microscopy ; morphological characteristics ; Morphology ; Physical characteristics ; pleural mesothelioma</subject><ispartof>Pathology international, 2019-11, Vol.69 (11), p.637-645</ispartof><rights>2019 Japanese Society of Pathology and John Wiley & Sons Australia, Ltd</rights><rights>2019 Japanese Society of Pathology and John Wiley & Sons Australia, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3772-dd53f1b7b7c2645a3455e0d5e87227e85505961c3827e4d0491496c457d599bf3</citedby><cites>FETCH-LOGICAL-c3772-dd53f1b7b7c2645a3455e0d5e87227e85505961c3827e4d0491496c457d599bf3</cites><orcidid>0000-0002-0691-1222</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fpin.12862$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fpin.12862$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31580004$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matsumoto, Shinji</creatorcontrib><creatorcontrib>Hamasaki, Makoto</creatorcontrib><creatorcontrib>Kinoshita, Yoshiaki</creatorcontrib><creatorcontrib>Kamei, Toshiaki</creatorcontrib><creatorcontrib>Kawahara, Kunimitsu</creatorcontrib><creatorcontrib>Nabeshima, Kazuki</creatorcontrib><title>Morphological difference between pleural mesothelioma cells in effusion smears with either BAP1 loss or 9p21 homozygous deletion and reactive mesothelial cells without the gene alterations</title><title>Pathology international</title><addtitle>Pathol Int</addtitle><description>We previously characterized the morphological characteristics of malignant pleural mesothelioma (MPM) cells with 9p21 homozygous deletion (HD) using a combination of the virtual microscopy and fluorescence in situ hybridization (FISH). In this study, we investigated whether MPM cells with BRCA1‐associated protein 1 (BAP1) loss show the same morphological characteristics identified in MPM cells with 9p21 HD. MPM cells with either BAP1 loss detected by immunocytochemistry (ICC) or 9p21 HD detected by FISH were identified via virtual microscopy prior to ICC or FISH, followed by analysis and quantification of their morphological characteristics. MPM cells with BAP1 loss or 9p21 HD exhibited significantly more frequent cell‐in‐cell engulfment, multinucleation, and larger multicellular clusters composed of more than 10 cells than reactive mesothelial cells. In conclusion, MPM cells with BAP1 loss or 9p21 HD share similar cytological features, indicating that the same morphological criteria can be used to detect MPM cells harboring such genetic aberrations.</description><subject>9p21 homozygous deletion</subject><subject>BAP1 loss</subject><subject>BRCA1 protein</subject><subject>Breast cancer</subject><subject>Clonal deletion</subject><subject>Effusion</subject><subject>effusion smear</subject><subject>Fluorescence</subject><subject>Fluorescence in situ hybridization</subject><subject>Gene deletion</subject><subject>Immunocytochemistry</subject><subject>Mesothelioma</subject><subject>Microscopy</subject><subject>morphological characteristics</subject><subject>Morphology</subject><subject>Physical characteristics</subject><subject>pleural mesothelioma</subject><issn>1320-5463</issn><issn>1440-1827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kc1u1TAQhSNERUthwQugkdjAIq1_42TZVhQqtdAFrCMnntzryomDnXB1ebY-HA5pi4SEF_bI_ubMeE6WvaHkhKZ1OtrhhLKyYM-yIyoEyWnJ1PMUc0ZyKQp-mL2M8Y4QqnhBXmSHnMqSECKOsvsbH8atd35jW-3A2K7DgEOL0OC0QxxgdDiH9NRj9NMWnfW9hhadi2AHwK6bo_UDxB51iLCz0xYwbRjg_OyWgvMxgg9QjYzC1vf-137j5wgGHU5Loh4MBNTtZH_i3yKp4FpjEfTzBOkWNjggaDdh0EtqfJUddNpFfP1wHmffLz9-u_icX3_9dHVxdp23XCmWGyN5RxvVqJYVQmoupERiJJaKMYWllERWBW15mhoKQ0RFRVW0Qiojq6rp-HH2ftUdg_8xY5zq3salPT1g-kvNOEkKFZEkoe_-Qe_8HIbUXaJo0pWEFIn6sFJtSOMJ2NVjsL0O-5qSerG0TpbWfyxN7NsHxbnp0TyRjx4m4HQFdtbh_v9K9e3Vl1XyN0VKrWw</recordid><startdate>201911</startdate><enddate>201911</enddate><creator>Matsumoto, Shinji</creator><creator>Hamasaki, Makoto</creator><creator>Kinoshita, Yoshiaki</creator><creator>Kamei, Toshiaki</creator><creator>Kawahara, Kunimitsu</creator><creator>Nabeshima, Kazuki</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0691-1222</orcidid></search><sort><creationdate>201911</creationdate><title>Morphological difference between pleural mesothelioma cells in effusion smears with either BAP1 loss or 9p21 homozygous deletion and reactive mesothelial cells without the gene alterations</title><author>Matsumoto, Shinji ; Hamasaki, Makoto ; Kinoshita, Yoshiaki ; Kamei, Toshiaki ; Kawahara, Kunimitsu ; Nabeshima, Kazuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3772-dd53f1b7b7c2645a3455e0d5e87227e85505961c3827e4d0491496c457d599bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>9p21 homozygous deletion</topic><topic>BAP1 loss</topic><topic>BRCA1 protein</topic><topic>Breast cancer</topic><topic>Clonal deletion</topic><topic>Effusion</topic><topic>effusion smear</topic><topic>Fluorescence</topic><topic>Fluorescence in situ hybridization</topic><topic>Gene deletion</topic><topic>Immunocytochemistry</topic><topic>Mesothelioma</topic><topic>Microscopy</topic><topic>morphological characteristics</topic><topic>Morphology</topic><topic>Physical characteristics</topic><topic>pleural mesothelioma</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matsumoto, Shinji</creatorcontrib><creatorcontrib>Hamasaki, Makoto</creatorcontrib><creatorcontrib>Kinoshita, Yoshiaki</creatorcontrib><creatorcontrib>Kamei, Toshiaki</creatorcontrib><creatorcontrib>Kawahara, Kunimitsu</creatorcontrib><creatorcontrib>Nabeshima, Kazuki</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Pathology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matsumoto, Shinji</au><au>Hamasaki, Makoto</au><au>Kinoshita, Yoshiaki</au><au>Kamei, Toshiaki</au><au>Kawahara, Kunimitsu</au><au>Nabeshima, Kazuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Morphological difference between pleural mesothelioma cells in effusion smears with either BAP1 loss or 9p21 homozygous deletion and reactive mesothelial cells without the gene alterations</atitle><jtitle>Pathology international</jtitle><addtitle>Pathol Int</addtitle><date>2019-11</date><risdate>2019</risdate><volume>69</volume><issue>11</issue><spage>637</spage><epage>645</epage><pages>637-645</pages><issn>1320-5463</issn><eissn>1440-1827</eissn><abstract>We previously characterized the morphological characteristics of malignant pleural mesothelioma (MPM) cells with 9p21 homozygous deletion (HD) using a combination of the virtual microscopy and fluorescence in situ hybridization (FISH). In this study, we investigated whether MPM cells with BRCA1‐associated protein 1 (BAP1) loss show the same morphological characteristics identified in MPM cells with 9p21 HD. MPM cells with either BAP1 loss detected by immunocytochemistry (ICC) or 9p21 HD detected by FISH were identified via virtual microscopy prior to ICC or FISH, followed by analysis and quantification of their morphological characteristics. MPM cells with BAP1 loss or 9p21 HD exhibited significantly more frequent cell‐in‐cell engulfment, multinucleation, and larger multicellular clusters composed of more than 10 cells than reactive mesothelial cells. In conclusion, MPM cells with BAP1 loss or 9p21 HD share similar cytological features, indicating that the same morphological criteria can be used to detect MPM cells harboring such genetic aberrations.</abstract><cop>Australia</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31580004</pmid><doi>10.1111/pin.12862</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-0691-1222</orcidid></addata></record> |
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| subjects | 9p21 homozygous deletion BAP1 loss BRCA1 protein Breast cancer Clonal deletion Effusion effusion smear Fluorescence Fluorescence in situ hybridization Gene deletion Immunocytochemistry Mesothelioma Microscopy morphological characteristics Morphology Physical characteristics pleural mesothelioma |
| title | Morphological difference between pleural mesothelioma cells in effusion smears with either BAP1 loss or 9p21 homozygous deletion and reactive mesothelial cells without the gene alterations |
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