Efficacy of Zea mays L. for the management of marble effluent contaminated soil under citric acid amendment; morpho-physiological and biochemical response

The adverse industrial activities discharged contaminated wastewater directly into the water bodies that contain toxic substances such as heavy metals. The contours use of marble industrial effluents may affect the fertility of soil and crop growth. The present study was conducted to investigate the toxic effects of marble industrial effluents (M.E) on Zea mays L under the exogenous application of citric acid (CA) with different combinations such as marble industrial effluent (0, 30%, 60%, 100%) diluted with distilled water and CA (10 mM). The results showed significant decrease in the growth of Zea mays with increasing concentration of marble industrial effluent. The maximum reduction in plant height, root length, number of leaves, leaf area and fresh and dry biomass was observed at the application of 100% M.E as compared to control. Similar to growth conditions the photosynthetic machinery and the activities of antioxidant enzymes (Superoxide dismutase (SOD), Peroxidases (POD), Catalases (CAT), Ascorbate peroxidase (APX)) was also decreased with increasing concentration of M.E. The application of CA significantly alleviated the M.E induced toxic effect on Zea mays and ameliorated the growth, biomass, photosynthesis and antioxidant enzymes activities by reducing the production of reactive oxygen species. The C.A application also enhanced the heavy metal content such as chromium (Cr), cadmium (Cd), Zinc (Zn) in different parts of Zea mays. The results concluded that the Zea mays tolerant varieties can be a potential candidate for the M.E irrigated soil and might be suitable for the phyto-extraction of Cr, Cd and Zn. [Display omitted] •Marble effluents altered biochemical and agronomic traits of Zea mays.•Citric acid improved photosynthetic pigments and antioxidant defense system.•Citric acid enhanced plant growth and biomass by higher nutrient uptake.•Citric acid enhanced heavy metals availability and uptake in Zea mays.