Therapy with multi-epitope virus-like particles of B19 parvovirus reduce tumor growth and lung metastasis in an aggressive breast cancer mouse model
Triple-negative breast cancer is a major health problem that lacks molecular targets for therapy. Neoepitopes represent a viable option to induce antitumor immune responses, but they have limitations, such as low immunogenicity and tolerance induction. Parvovirus B19 virus-like particles may be used...
Gespeichert in:
| Veröffentlicht in: | Vaccine 2019-11, Vol.37 (49), p.7256-7268 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 7268 |
|---|---|
| container_issue | 49 |
| container_start_page | 7256 |
| container_title | Vaccine |
| container_volume | 37 |
| creator | Jiménez-Chávez, Ángel de Jesús Moreno-Fierros, Leticia Bustos-Jaimes, Ismael |
| description | Triple-negative breast cancer is a major health problem that lacks molecular targets for therapy. Neoepitopes represent a viable option to induce antitumor immune responses, but they have limitations, such as low immunogenicity and tolerance induction. Parvovirus B19 virus-like particles may be used to deliver neoepitopes to prime cellular immunity. We designed and evaluated the therapeutic effect of VP2 B19-virus-like particles, with multi-neoepitopes, in a 4T1 breast cancer model. Balb/c mice received four therapeutic immunizations with multi-neoepitopes-virus-like, wild type-virus-like, vehicle, or virus-like plus Cry1Ac adjuvant particles, intraperitoneally and peritumorally. Tumor growth, lung macro-metastasis, and specific immune responses were evaluated. Therapeutic administration of multi-epitopes virus-like particles significantly delayed tumor growth and decreased the lung macro-metastasis number, in comparison to treatment with wild type-virus-like particles, which surprisingly also elicited antitumoral effects that were improved with the adjuvant. Only treatments with multi-epitope virus-like particles induced specific proliferative responses of CD8 and CD4 T lymphocytes and Granzyme-B production in lymphatic nodes local to the tumor. Treatment with recombinant multiple neoepitopes-virus-like particles induced specific cellular responses, inhibited tumor growth and macro-metastasis, thus B19-virus-like particles may function as an effective delivery system for neoepitopes for personalized immunotherapy. |
| doi_str_mv | 10.1016/j.vaccine.2019.09.068 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2299775168</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0264410X19312964</els_id><sourcerecordid>2299775168</sourcerecordid><originalsourceid>FETCH-LOGICAL-c370t-d1b5d477b321bf1fdbbda69bbecefc28d62c26f5927e5bfd5e2e078be2be3d5d3</originalsourceid><addsrcrecordid>eNqFkc1q3DAUhUVJoZNpHqEgyKYbTyV5JNmrkIYmDQS6SSE7oZ_riaa25UiyQ96jD1xNJqtsCgcJrj4d7r0HoS-UbCih4tt-s2hr_QgbRmi7IUWi-YBWtJF1xThtTtCKMLGttpQ8fEKnKe0JIbym7Qr9vX-EqKcX_OzzIx7mPvsKJp_DBHjxcU5V7_8AnnTM3vaQcOjwd9oeCkt4BXAEN1vAeR5CxLsYnouRHh3u53GHB8g6FfmE_VjKWO92EVLyC2ATobxhq0cLEQ9hTlBOB_1n9LHTfYKzt3uNfl__uL_6Wd39urm9uryrbC1Jrhw13G2lNDWjpqOdM8Zp0RoDFjrLGieYZaLjLZPATec4MCCyMcAM1I67eo2-Hn2nGJ5mSFkNPlnoez1C6UYx1rZSciqagp6_Q_dhjmPpTrGaMinaWohC8SNlY0gpQqem6AcdXxQl6pCV2qu3rNQhK0WKXt0vjv-gTLt4iCpZD2UtzkewWbng_-PwD-1dpHo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2312769366</pqid></control><display><type>article</type><title>Therapy with multi-epitope virus-like particles of B19 parvovirus reduce tumor growth and lung metastasis in an aggressive breast cancer mouse model</title><source>Elsevier ScienceDirect Journals Complete</source><source>ProQuest Central UK/Ireland</source><creator>Jiménez-Chávez, Ángel de Jesús ; Moreno-Fierros, Leticia ; Bustos-Jaimes, Ismael</creator><creatorcontrib>Jiménez-Chávez, Ángel de Jesús ; Moreno-Fierros, Leticia ; Bustos-Jaimes, Ismael</creatorcontrib><description>Triple-negative breast cancer is a major health problem that lacks molecular targets for therapy. Neoepitopes represent a viable option to induce antitumor immune responses, but they have limitations, such as low immunogenicity and tolerance induction. Parvovirus B19 virus-like particles may be used to deliver neoepitopes to prime cellular immunity. We designed and evaluated the therapeutic effect of VP2 B19-virus-like particles, with multi-neoepitopes, in a 4T1 breast cancer model. Balb/c mice received four therapeutic immunizations with multi-neoepitopes-virus-like, wild type-virus-like, vehicle, or virus-like plus Cry1Ac adjuvant particles, intraperitoneally and peritumorally. Tumor growth, lung macro-metastasis, and specific immune responses were evaluated. Therapeutic administration of multi-epitopes virus-like particles significantly delayed tumor growth and decreased the lung macro-metastasis number, in comparison to treatment with wild type-virus-like particles, which surprisingly also elicited antitumoral effects that were improved with the adjuvant. Only treatments with multi-epitope virus-like particles induced specific proliferative responses of CD8 and CD4 T lymphocytes and Granzyme-B production in lymphatic nodes local to the tumor. Treatment with recombinant multiple neoepitopes-virus-like particles induced specific cellular responses, inhibited tumor growth and macro-metastasis, thus B19-virus-like particles may function as an effective delivery system for neoepitopes for personalized immunotherapy.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2019.09.068</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Anticancer properties ; Antigens ; Antitumor activity ; Breast cancer ; Cancer therapies ; CD4 antigen ; CD8 antigen ; Cell-mediated immunity ; Cry1Ac toxin ; Cytokines ; Cytotoxicity ; Epitopes ; Immune response ; Immunogenicity ; Immunological tolerance ; Immunotherapy ; Lung cancer ; Lungs ; Lymphatic system ; Lymphocytes ; Lymphocytes B ; Lymphocytes T ; Metastases ; Metastasis ; Neoepitopes ; Parvoviruses ; Proteins ; Therapy ; Triple negative breast cancer ; Tumors ; Vaccine ; Virus-like particles ; Viruses</subject><ispartof>Vaccine, 2019-11, Vol.37 (49), p.7256-7268</ispartof><rights>2019 Elsevier Ltd</rights><rights>2019. Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-d1b5d477b321bf1fdbbda69bbecefc28d62c26f5927e5bfd5e2e078be2be3d5d3</citedby><cites>FETCH-LOGICAL-c370t-d1b5d477b321bf1fdbbda69bbecefc28d62c26f5927e5bfd5e2e078be2be3d5d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/2312769366?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995,64385,64387,64389,72469</link.rule.ids></links><search><creatorcontrib>Jiménez-Chávez, Ángel de Jesús</creatorcontrib><creatorcontrib>Moreno-Fierros, Leticia</creatorcontrib><creatorcontrib>Bustos-Jaimes, Ismael</creatorcontrib><title>Therapy with multi-epitope virus-like particles of B19 parvovirus reduce tumor growth and lung metastasis in an aggressive breast cancer mouse model</title><title>Vaccine</title><description>Triple-negative breast cancer is a major health problem that lacks molecular targets for therapy. Neoepitopes represent a viable option to induce antitumor immune responses, but they have limitations, such as low immunogenicity and tolerance induction. Parvovirus B19 virus-like particles may be used to deliver neoepitopes to prime cellular immunity. We designed and evaluated the therapeutic effect of VP2 B19-virus-like particles, with multi-neoepitopes, in a 4T1 breast cancer model. Balb/c mice received four therapeutic immunizations with multi-neoepitopes-virus-like, wild type-virus-like, vehicle, or virus-like plus Cry1Ac adjuvant particles, intraperitoneally and peritumorally. Tumor growth, lung macro-metastasis, and specific immune responses were evaluated. Therapeutic administration of multi-epitopes virus-like particles significantly delayed tumor growth and decreased the lung macro-metastasis number, in comparison to treatment with wild type-virus-like particles, which surprisingly also elicited antitumoral effects that were improved with the adjuvant. Only treatments with multi-epitope virus-like particles induced specific proliferative responses of CD8 and CD4 T lymphocytes and Granzyme-B production in lymphatic nodes local to the tumor. Treatment with recombinant multiple neoepitopes-virus-like particles induced specific cellular responses, inhibited tumor growth and macro-metastasis, thus B19-virus-like particles may function as an effective delivery system for neoepitopes for personalized immunotherapy.</description><subject>Anticancer properties</subject><subject>Antigens</subject><subject>Antitumor activity</subject><subject>Breast cancer</subject><subject>Cancer therapies</subject><subject>CD4 antigen</subject><subject>CD8 antigen</subject><subject>Cell-mediated immunity</subject><subject>Cry1Ac toxin</subject><subject>Cytokines</subject><subject>Cytotoxicity</subject><subject>Epitopes</subject><subject>Immune response</subject><subject>Immunogenicity</subject><subject>Immunological tolerance</subject><subject>Immunotherapy</subject><subject>Lung cancer</subject><subject>Lungs</subject><subject>Lymphatic system</subject><subject>Lymphocytes</subject><subject>Lymphocytes B</subject><subject>Lymphocytes T</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>Neoepitopes</subject><subject>Parvoviruses</subject><subject>Proteins</subject><subject>Therapy</subject><subject>Triple negative breast cancer</subject><subject>Tumors</subject><subject>Vaccine</subject><subject>Virus-like particles</subject><subject>Viruses</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkc1q3DAUhUVJoZNpHqEgyKYbTyV5JNmrkIYmDQS6SSE7oZ_riaa25UiyQ96jD1xNJqtsCgcJrj4d7r0HoS-UbCih4tt-s2hr_QgbRmi7IUWi-YBWtJF1xThtTtCKMLGttpQ8fEKnKe0JIbym7Qr9vX-EqKcX_OzzIx7mPvsKJp_DBHjxcU5V7_8AnnTM3vaQcOjwd9oeCkt4BXAEN1vAeR5CxLsYnouRHh3u53GHB8g6FfmE_VjKWO92EVLyC2ATobxhq0cLEQ9hTlBOB_1n9LHTfYKzt3uNfl__uL_6Wd39urm9uryrbC1Jrhw13G2lNDWjpqOdM8Zp0RoDFjrLGieYZaLjLZPATec4MCCyMcAM1I67eo2-Hn2nGJ5mSFkNPlnoez1C6UYx1rZSciqagp6_Q_dhjmPpTrGaMinaWohC8SNlY0gpQqem6AcdXxQl6pCV2qu3rNQhK0WKXt0vjv-gTLt4iCpZD2UtzkewWbng_-PwD-1dpHo</recordid><startdate>20191120</startdate><enddate>20191120</enddate><creator>Jiménez-Chávez, Ángel de Jesús</creator><creator>Moreno-Fierros, Leticia</creator><creator>Bustos-Jaimes, Ismael</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20191120</creationdate><title>Therapy with multi-epitope virus-like particles of B19 parvovirus reduce tumor growth and lung metastasis in an aggressive breast cancer mouse model</title><author>Jiménez-Chávez, Ángel de Jesús ; Moreno-Fierros, Leticia ; Bustos-Jaimes, Ismael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-d1b5d477b321bf1fdbbda69bbecefc28d62c26f5927e5bfd5e2e078be2be3d5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Anticancer properties</topic><topic>Antigens</topic><topic>Antitumor activity</topic><topic>Breast cancer</topic><topic>Cancer therapies</topic><topic>CD4 antigen</topic><topic>CD8 antigen</topic><topic>Cell-mediated immunity</topic><topic>Cry1Ac toxin</topic><topic>Cytokines</topic><topic>Cytotoxicity</topic><topic>Epitopes</topic><topic>Immune response</topic><topic>Immunogenicity</topic><topic>Immunological tolerance</topic><topic>Immunotherapy</topic><topic>Lung cancer</topic><topic>Lungs</topic><topic>Lymphatic system</topic><topic>Lymphocytes</topic><topic>Lymphocytes B</topic><topic>Lymphocytes T</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>Neoepitopes</topic><topic>Parvoviruses</topic><topic>Proteins</topic><topic>Therapy</topic><topic>Triple negative breast cancer</topic><topic>Tumors</topic><topic>Vaccine</topic><topic>Virus-like particles</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiménez-Chávez, Ángel de Jesús</creatorcontrib><creatorcontrib>Moreno-Fierros, Leticia</creatorcontrib><creatorcontrib>Bustos-Jaimes, Ismael</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nursing & Allied Health Database</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Healthcare Administration Database (Alumni)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Healthcare Administration Database</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiménez-Chávez, Ángel de Jesús</au><au>Moreno-Fierros, Leticia</au><au>Bustos-Jaimes, Ismael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Therapy with multi-epitope virus-like particles of B19 parvovirus reduce tumor growth and lung metastasis in an aggressive breast cancer mouse model</atitle><jtitle>Vaccine</jtitle><date>2019-11-20</date><risdate>2019</risdate><volume>37</volume><issue>49</issue><spage>7256</spage><epage>7268</epage><pages>7256-7268</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><abstract>Triple-negative breast cancer is a major health problem that lacks molecular targets for therapy. Neoepitopes represent a viable option to induce antitumor immune responses, but they have limitations, such as low immunogenicity and tolerance induction. Parvovirus B19 virus-like particles may be used to deliver neoepitopes to prime cellular immunity. We designed and evaluated the therapeutic effect of VP2 B19-virus-like particles, with multi-neoepitopes, in a 4T1 breast cancer model. Balb/c mice received four therapeutic immunizations with multi-neoepitopes-virus-like, wild type-virus-like, vehicle, or virus-like plus Cry1Ac adjuvant particles, intraperitoneally and peritumorally. Tumor growth, lung macro-metastasis, and specific immune responses were evaluated. Therapeutic administration of multi-epitopes virus-like particles significantly delayed tumor growth and decreased the lung macro-metastasis number, in comparison to treatment with wild type-virus-like particles, which surprisingly also elicited antitumoral effects that were improved with the adjuvant. Only treatments with multi-epitope virus-like particles induced specific proliferative responses of CD8 and CD4 T lymphocytes and Granzyme-B production in lymphatic nodes local to the tumor. Treatment with recombinant multiple neoepitopes-virus-like particles induced specific cellular responses, inhibited tumor growth and macro-metastasis, thus B19-virus-like particles may function as an effective delivery system for neoepitopes for personalized immunotherapy.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.vaccine.2019.09.068</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0264-410X |
| ispartof | Vaccine, 2019-11, Vol.37 (49), p.7256-7268 |
| issn | 0264-410X 1873-2518 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2299775168 |
| source | Elsevier ScienceDirect Journals Complete; ProQuest Central UK/Ireland |
| subjects | Anticancer properties Antigens Antitumor activity Breast cancer Cancer therapies CD4 antigen CD8 antigen Cell-mediated immunity Cry1Ac toxin Cytokines Cytotoxicity Epitopes Immune response Immunogenicity Immunological tolerance Immunotherapy Lung cancer Lungs Lymphatic system Lymphocytes Lymphocytes B Lymphocytes T Metastases Metastasis Neoepitopes Parvoviruses Proteins Therapy Triple negative breast cancer Tumors Vaccine Virus-like particles Viruses |
| title | Therapy with multi-epitope virus-like particles of B19 parvovirus reduce tumor growth and lung metastasis in an aggressive breast cancer mouse model |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T13%3A03%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Therapy%20with%20multi-epitope%20virus-like%20particles%20of%20B19%20parvovirus%20reduce%20tumor%20growth%20and%20lung%20metastasis%20in%20an%20aggressive%20breast%20cancer%20mouse%20model&rft.jtitle=Vaccine&rft.au=Jim%C3%A9nez-Ch%C3%A1vez,%20%C3%81ngel%20de%20Jes%C3%BAs&rft.date=2019-11-20&rft.volume=37&rft.issue=49&rft.spage=7256&rft.epage=7268&rft.pages=7256-7268&rft.issn=0264-410X&rft.eissn=1873-2518&rft_id=info:doi/10.1016/j.vaccine.2019.09.068&rft_dat=%3Cproquest_cross%3E2299775168%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2312769366&rft_id=info:pmid/&rft_els_id=S0264410X19312964&rfr_iscdi=true |