Changes of serum lipopolysaccharide, inflammatory factors, and cecal microbiota in obese rats with type 2 diabetes induced by Roux-en-Y gastric bypass
•Roux-en-Y gastric bypass (RYGB) significantly reduced fasting plasma glucose and weight levels in obese rats with type 2 diabetes.•Interleukin-1, -6, and tumor necrosis factor alpha levels were obviously decreased after RYGB.•Lipopolysaccharide levels of peripheral and superior mesenteric venous se...
Gespeichert in:
| Veröffentlicht in: | Nutrition (Burbank, Los Angeles County, Calif.) Los Angeles County, Calif.), 2019-11, Vol.67-68, p.110565-110565, Article 110565 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 110565 |
|---|---|
| container_issue | |
| container_start_page | 110565 |
| container_title | Nutrition (Burbank, Los Angeles County, Calif.) |
| container_volume | 67-68 |
| creator | Wu, Jiao Zhang, Peng-Bo Ren, Ze-Qiang Zhou, Fang Hu, Hui-Hui Zhang, Hong Xue, Kai-Kai Xu, Pan Shao, Xiao-Qing |
| description | •Roux-en-Y gastric bypass (RYGB) significantly reduced fasting plasma glucose and weight levels in obese rats with type 2 diabetes.•Interleukin-1, -6, and tumor necrosis factor alpha levels were obviously decreased after RYGB.•Lipopolysaccharide levels of peripheral and superior mesenteric venous serum were markedly reduced.•After RYGB, Bifidobacterium appeared and increased significantly in cecum microbiota.
Previous studies have shown that Roux-en-Y gastric bypass (RYGB) leads to rapid regression of obesity and type 2 diabetes (T2D). However, the underlying mechanism remains unclear. This study aimed to investigate the effect of RYGB on serum lipopolysaccharide (LPS), interleukin (IL)-1, IL-6, tumor necrosis factor alpha (TNF-α), and cecal microbiota in obese rats with T2D.
Obese Sprague-Dawley rats with T2D were randomly divided into RYGB diabetes operation (DO; n = 8), diabetes sham operation (DS; n = 8), and diabetic control (DC; n = 8) groups. Healthy Sprague-Dawley rats were grouped as normal control (NC; n = 8). Fasting plasma glucose and body weight were measured. The levels of peripheral serum LPS, IL-1, IL-6, and TNF-α were measured by enzyme-linked immunosorbent assay. The rats were sacrificed 12 wk after operation. Subsequently, a superior mesenteric venous blood sample was taken to measure serum LPS levels by enzyme-linked immunosorbent assay. The cecal contents of the DO and DS groups were taken to extract metagenomic DNA per the genomic DNA standardization procedure. The V4 region of the 16 S rRNA was sequenced with the Illumina Hiseq sequencing platform to compare the structure and relative abundance of cecal microbiota between the DO and DS groups.
Twelve weeks after operation in the DO group, fasting plasma glucose and body weight showed a significant decrease (P < 0.05). Moreover, the levels of peripheral serum LPS, IL-1, IL-6, and TNF-α were obviously decreased (P < 0.05). A change in the LPS level of superior mesenteric venous blood also revealed a dramatic decrease (P < 0.05). Additionally, RYGB resulted in a shift of cecal microbiota in obese rats with T2D.
Hypoglycemic effects after RYGB may be associated with improved levels of LPS, IL-1, IL-6, and TNF-α. Changes in the structure of cecal microbiota may also play an important role. |
| doi_str_mv | 10.1016/j.nut.2019.110565 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2299144658</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0899900719301224</els_id><sourcerecordid>2299144658</sourcerecordid><originalsourceid>FETCH-LOGICAL-c381t-d142955cf4a12c722521e0529f4efffc1f582ba557c292028147a70b5b78ebcc3</originalsourceid><addsrcrecordid>eNp9kU2L1TAUhoMoznX0B7iRgBsX02tO2rQNruTiFwwIogtXIUlP5ubSNjVJ1f4Rf68Z7ujChasDh-e8Ce9DyFNge2DQvjzt5zXvOQO5B2CiFffIDvquroA3zX2yY72UlWSsuyCPUjoxVshWPiQXNYgWOBM78utw1PMNJhocTRjXiY5-CUsYt6StPeroB7yifnajniadQ9yo07bMdEX1PFCLVo908jYG40PWBaXBYEIadU70h89HmrcFKaeD1wZzecrPw2pxoGajn8L6s8K5-kpvdMrR27JcdEqPyQOnx4RP7uYl-fL2zefD--r647sPh9fXla17yNUADZdCWNdo4LbjXHBAJrh0DTrnLDjRc6OF6CyXnPEemk53zAjT9WisrS_Ji3PuEsO3FVNWk08Wx1HPGNakOJcSmqYVfUGf_4Oewhrn8jvFS5-y5oKJQsGZKoWkFNGpJfpJx00BU7fS1EkVaepWmjpLKzfP7pJXM-Hw9-KPpQK8OgNYqvjuMapkPc6lQx_RZjUE_5_43zYJqJY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2315932505</pqid></control><display><type>article</type><title>Changes of serum lipopolysaccharide, inflammatory factors, and cecal microbiota in obese rats with type 2 diabetes induced by Roux-en-Y gastric bypass</title><source>Elsevier ScienceDirect Journals Complete</source><source>ProQuest Central UK/Ireland</source><creator>Wu, Jiao ; Zhang, Peng-Bo ; Ren, Ze-Qiang ; Zhou, Fang ; Hu, Hui-Hui ; Zhang, Hong ; Xue, Kai-Kai ; Xu, Pan ; Shao, Xiao-Qing</creator><creatorcontrib>Wu, Jiao ; Zhang, Peng-Bo ; Ren, Ze-Qiang ; Zhou, Fang ; Hu, Hui-Hui ; Zhang, Hong ; Xue, Kai-Kai ; Xu, Pan ; Shao, Xiao-Qing</creatorcontrib><description>•Roux-en-Y gastric bypass (RYGB) significantly reduced fasting plasma glucose and weight levels in obese rats with type 2 diabetes.•Interleukin-1, -6, and tumor necrosis factor alpha levels were obviously decreased after RYGB.•Lipopolysaccharide levels of peripheral and superior mesenteric venous serum were markedly reduced.•After RYGB, Bifidobacterium appeared and increased significantly in cecum microbiota.
Previous studies have shown that Roux-en-Y gastric bypass (RYGB) leads to rapid regression of obesity and type 2 diabetes (T2D). However, the underlying mechanism remains unclear. This study aimed to investigate the effect of RYGB on serum lipopolysaccharide (LPS), interleukin (IL)-1, IL-6, tumor necrosis factor alpha (TNF-α), and cecal microbiota in obese rats with T2D.
Obese Sprague-Dawley rats with T2D were randomly divided into RYGB diabetes operation (DO; n = 8), diabetes sham operation (DS; n = 8), and diabetic control (DC; n = 8) groups. Healthy Sprague-Dawley rats were grouped as normal control (NC; n = 8). Fasting plasma glucose and body weight were measured. The levels of peripheral serum LPS, IL-1, IL-6, and TNF-α were measured by enzyme-linked immunosorbent assay. The rats were sacrificed 12 wk after operation. Subsequently, a superior mesenteric venous blood sample was taken to measure serum LPS levels by enzyme-linked immunosorbent assay. The cecal contents of the DO and DS groups were taken to extract metagenomic DNA per the genomic DNA standardization procedure. The V4 region of the 16 S rRNA was sequenced with the Illumina Hiseq sequencing platform to compare the structure and relative abundance of cecal microbiota between the DO and DS groups.
Twelve weeks after operation in the DO group, fasting plasma glucose and body weight showed a significant decrease (P < 0.05). Moreover, the levels of peripheral serum LPS, IL-1, IL-6, and TNF-α were obviously decreased (P < 0.05). A change in the LPS level of superior mesenteric venous blood also revealed a dramatic decrease (P < 0.05). Additionally, RYGB resulted in a shift of cecal microbiota in obese rats with T2D.
Hypoglycemic effects after RYGB may be associated with improved levels of LPS, IL-1, IL-6, and TNF-α. Changes in the structure of cecal microbiota may also play an important role.</description><identifier>ISSN: 0899-9007</identifier><identifier>EISSN: 1873-1244</identifier><identifier>DOI: 10.1016/j.nut.2019.110565</identifier><identifier>PMID: 31561205</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Bacteria ; Blood ; Body weight ; Cecal microbiota ; Cecum ; Deoxyribonucleic acid ; Diabetes ; Diabetes mellitus (non-insulin dependent) ; Diet ; DNA ; Enzyme-linked immunosorbent assay ; Experiments ; Fasting ; Gastric bypass ; Gastrointestinal surgery ; Gene expression ; Glucose ; Inflammation ; Inflammatory factors ; Insulin resistance ; Interleukin 1 ; Interleukin 6 ; Levels ; Lipopolysaccharide ; Lipopolysaccharides ; Medical research ; Metabolism ; Microbiota ; Obesity ; Probiotics ; Relative abundance ; Roux-en-Y gastric bypass ; rRNA ; Standardization ; Tumor necrosis factor-TNF ; Tumor necrosis factor-α ; Type 2 diabetes ; Veins & arteries ; Weight control</subject><ispartof>Nutrition (Burbank, Los Angeles County, Calif.), 2019-11, Vol.67-68, p.110565-110565, Article 110565</ispartof><rights>2019</rights><rights>Copyright © 2019. Published by Elsevier Inc.</rights><rights>Copyright Elsevier Limited Nov 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-d142955cf4a12c722521e0529f4efffc1f582ba557c292028147a70b5b78ebcc3</citedby><cites>FETCH-LOGICAL-c381t-d142955cf4a12c722521e0529f4efffc1f582ba557c292028147a70b5b78ebcc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/2315932505?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995,64385,64387,64389,72469</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31561205$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Jiao</creatorcontrib><creatorcontrib>Zhang, Peng-Bo</creatorcontrib><creatorcontrib>Ren, Ze-Qiang</creatorcontrib><creatorcontrib>Zhou, Fang</creatorcontrib><creatorcontrib>Hu, Hui-Hui</creatorcontrib><creatorcontrib>Zhang, Hong</creatorcontrib><creatorcontrib>Xue, Kai-Kai</creatorcontrib><creatorcontrib>Xu, Pan</creatorcontrib><creatorcontrib>Shao, Xiao-Qing</creatorcontrib><title>Changes of serum lipopolysaccharide, inflammatory factors, and cecal microbiota in obese rats with type 2 diabetes induced by Roux-en-Y gastric bypass</title><title>Nutrition (Burbank, Los Angeles County, Calif.)</title><addtitle>Nutrition</addtitle><description>•Roux-en-Y gastric bypass (RYGB) significantly reduced fasting plasma glucose and weight levels in obese rats with type 2 diabetes.•Interleukin-1, -6, and tumor necrosis factor alpha levels were obviously decreased after RYGB.•Lipopolysaccharide levels of peripheral and superior mesenteric venous serum were markedly reduced.•After RYGB, Bifidobacterium appeared and increased significantly in cecum microbiota.
Previous studies have shown that Roux-en-Y gastric bypass (RYGB) leads to rapid regression of obesity and type 2 diabetes (T2D). However, the underlying mechanism remains unclear. This study aimed to investigate the effect of RYGB on serum lipopolysaccharide (LPS), interleukin (IL)-1, IL-6, tumor necrosis factor alpha (TNF-α), and cecal microbiota in obese rats with T2D.
Obese Sprague-Dawley rats with T2D were randomly divided into RYGB diabetes operation (DO; n = 8), diabetes sham operation (DS; n = 8), and diabetic control (DC; n = 8) groups. Healthy Sprague-Dawley rats were grouped as normal control (NC; n = 8). Fasting plasma glucose and body weight were measured. The levels of peripheral serum LPS, IL-1, IL-6, and TNF-α were measured by enzyme-linked immunosorbent assay. The rats were sacrificed 12 wk after operation. Subsequently, a superior mesenteric venous blood sample was taken to measure serum LPS levels by enzyme-linked immunosorbent assay. The cecal contents of the DO and DS groups were taken to extract metagenomic DNA per the genomic DNA standardization procedure. The V4 region of the 16 S rRNA was sequenced with the Illumina Hiseq sequencing platform to compare the structure and relative abundance of cecal microbiota between the DO and DS groups.
Twelve weeks after operation in the DO group, fasting plasma glucose and body weight showed a significant decrease (P < 0.05). Moreover, the levels of peripheral serum LPS, IL-1, IL-6, and TNF-α were obviously decreased (P < 0.05). A change in the LPS level of superior mesenteric venous blood also revealed a dramatic decrease (P < 0.05). Additionally, RYGB resulted in a shift of cecal microbiota in obese rats with T2D.
Hypoglycemic effects after RYGB may be associated with improved levels of LPS, IL-1, IL-6, and TNF-α. Changes in the structure of cecal microbiota may also play an important role.</description><subject>Bacteria</subject><subject>Blood</subject><subject>Body weight</subject><subject>Cecal microbiota</subject><subject>Cecum</subject><subject>Deoxyribonucleic acid</subject><subject>Diabetes</subject><subject>Diabetes mellitus (non-insulin dependent)</subject><subject>Diet</subject><subject>DNA</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Experiments</subject><subject>Fasting</subject><subject>Gastric bypass</subject><subject>Gastrointestinal surgery</subject><subject>Gene expression</subject><subject>Glucose</subject><subject>Inflammation</subject><subject>Inflammatory factors</subject><subject>Insulin resistance</subject><subject>Interleukin 1</subject><subject>Interleukin 6</subject><subject>Levels</subject><subject>Lipopolysaccharide</subject><subject>Lipopolysaccharides</subject><subject>Medical research</subject><subject>Metabolism</subject><subject>Microbiota</subject><subject>Obesity</subject><subject>Probiotics</subject><subject>Relative abundance</subject><subject>Roux-en-Y gastric bypass</subject><subject>rRNA</subject><subject>Standardization</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tumor necrosis factor-α</subject><subject>Type 2 diabetes</subject><subject>Veins & arteries</subject><subject>Weight control</subject><issn>0899-9007</issn><issn>1873-1244</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9kU2L1TAUhoMoznX0B7iRgBsX02tO2rQNruTiFwwIogtXIUlP5ubSNjVJ1f4Rf68Z7ujChasDh-e8Ce9DyFNge2DQvjzt5zXvOQO5B2CiFffIDvquroA3zX2yY72UlWSsuyCPUjoxVshWPiQXNYgWOBM78utw1PMNJhocTRjXiY5-CUsYt6StPeroB7yifnajniadQ9yo07bMdEX1PFCLVo908jYG40PWBaXBYEIadU70h89HmrcFKaeD1wZzecrPw2pxoGajn8L6s8K5-kpvdMrR27JcdEqPyQOnx4RP7uYl-fL2zefD--r647sPh9fXla17yNUADZdCWNdo4LbjXHBAJrh0DTrnLDjRc6OF6CyXnPEemk53zAjT9WisrS_Ji3PuEsO3FVNWk08Wx1HPGNakOJcSmqYVfUGf_4Oewhrn8jvFS5-y5oKJQsGZKoWkFNGpJfpJx00BU7fS1EkVaepWmjpLKzfP7pJXM-Hw9-KPpQK8OgNYqvjuMapkPc6lQx_RZjUE_5_43zYJqJY</recordid><startdate>201911</startdate><enddate>201911</enddate><creator>Wu, Jiao</creator><creator>Zhang, Peng-Bo</creator><creator>Ren, Ze-Qiang</creator><creator>Zhou, Fang</creator><creator>Hu, Hui-Hui</creator><creator>Zhang, Hong</creator><creator>Xue, Kai-Kai</creator><creator>Xu, Pan</creator><creator>Shao, Xiao-Qing</creator><general>Elsevier Inc</general><general>Elsevier Limited</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RQ</scope><scope>7RV</scope><scope>7TS</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>ASE</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FPQ</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K6X</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>201911</creationdate><title>Changes of serum lipopolysaccharide, inflammatory factors, and cecal microbiota in obese rats with type 2 diabetes induced by Roux-en-Y gastric bypass</title><author>Wu, Jiao ; Zhang, Peng-Bo ; Ren, Ze-Qiang ; Zhou, Fang ; Hu, Hui-Hui ; Zhang, Hong ; Xue, Kai-Kai ; Xu, Pan ; Shao, Xiao-Qing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-d142955cf4a12c722521e0529f4efffc1f582ba557c292028147a70b5b78ebcc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Bacteria</topic><topic>Blood</topic><topic>Body weight</topic><topic>Cecal microbiota</topic><topic>Cecum</topic><topic>Deoxyribonucleic acid</topic><topic>Diabetes</topic><topic>Diabetes mellitus (non-insulin dependent)</topic><topic>Diet</topic><topic>DNA</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Experiments</topic><topic>Fasting</topic><topic>Gastric bypass</topic><topic>Gastrointestinal surgery</topic><topic>Gene expression</topic><topic>Glucose</topic><topic>Inflammation</topic><topic>Inflammatory factors</topic><topic>Insulin resistance</topic><topic>Interleukin 1</topic><topic>Interleukin 6</topic><topic>Levels</topic><topic>Lipopolysaccharide</topic><topic>Lipopolysaccharides</topic><topic>Medical research</topic><topic>Metabolism</topic><topic>Microbiota</topic><topic>Obesity</topic><topic>Probiotics</topic><topic>Relative abundance</topic><topic>Roux-en-Y gastric bypass</topic><topic>rRNA</topic><topic>Standardization</topic><topic>Tumor necrosis factor-TNF</topic><topic>Tumor necrosis factor-α</topic><topic>Type 2 diabetes</topic><topic>Veins & arteries</topic><topic>Weight control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Jiao</creatorcontrib><creatorcontrib>Zhang, Peng-Bo</creatorcontrib><creatorcontrib>Ren, Ze-Qiang</creatorcontrib><creatorcontrib>Zhou, Fang</creatorcontrib><creatorcontrib>Hu, Hui-Hui</creatorcontrib><creatorcontrib>Zhang, Hong</creatorcontrib><creatorcontrib>Xue, Kai-Kai</creatorcontrib><creatorcontrib>Xu, Pan</creatorcontrib><creatorcontrib>Shao, Xiao-Qing</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Career & Technical Education Database</collection><collection>Nursing & Allied Health Database</collection><collection>Physical Education Index</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Healthcare Administration Database (Alumni)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>British Nursing Index</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>British Nursing Index (BNI) (1985 to Present)</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>British Nursing Index</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Healthcare Administration Database</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Nutrition (Burbank, Los Angeles County, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Jiao</au><au>Zhang, Peng-Bo</au><au>Ren, Ze-Qiang</au><au>Zhou, Fang</au><au>Hu, Hui-Hui</au><au>Zhang, Hong</au><au>Xue, Kai-Kai</au><au>Xu, Pan</au><au>Shao, Xiao-Qing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Changes of serum lipopolysaccharide, inflammatory factors, and cecal microbiota in obese rats with type 2 diabetes induced by Roux-en-Y gastric bypass</atitle><jtitle>Nutrition (Burbank, Los Angeles County, Calif.)</jtitle><addtitle>Nutrition</addtitle><date>2019-11</date><risdate>2019</risdate><volume>67-68</volume><spage>110565</spage><epage>110565</epage><pages>110565-110565</pages><artnum>110565</artnum><issn>0899-9007</issn><eissn>1873-1244</eissn><abstract>•Roux-en-Y gastric bypass (RYGB) significantly reduced fasting plasma glucose and weight levels in obese rats with type 2 diabetes.•Interleukin-1, -6, and tumor necrosis factor alpha levels were obviously decreased after RYGB.•Lipopolysaccharide levels of peripheral and superior mesenteric venous serum were markedly reduced.•After RYGB, Bifidobacterium appeared and increased significantly in cecum microbiota.
Previous studies have shown that Roux-en-Y gastric bypass (RYGB) leads to rapid regression of obesity and type 2 diabetes (T2D). However, the underlying mechanism remains unclear. This study aimed to investigate the effect of RYGB on serum lipopolysaccharide (LPS), interleukin (IL)-1, IL-6, tumor necrosis factor alpha (TNF-α), and cecal microbiota in obese rats with T2D.
Obese Sprague-Dawley rats with T2D were randomly divided into RYGB diabetes operation (DO; n = 8), diabetes sham operation (DS; n = 8), and diabetic control (DC; n = 8) groups. Healthy Sprague-Dawley rats were grouped as normal control (NC; n = 8). Fasting plasma glucose and body weight were measured. The levels of peripheral serum LPS, IL-1, IL-6, and TNF-α were measured by enzyme-linked immunosorbent assay. The rats were sacrificed 12 wk after operation. Subsequently, a superior mesenteric venous blood sample was taken to measure serum LPS levels by enzyme-linked immunosorbent assay. The cecal contents of the DO and DS groups were taken to extract metagenomic DNA per the genomic DNA standardization procedure. The V4 region of the 16 S rRNA was sequenced with the Illumina Hiseq sequencing platform to compare the structure and relative abundance of cecal microbiota between the DO and DS groups.
Twelve weeks after operation in the DO group, fasting plasma glucose and body weight showed a significant decrease (P < 0.05). Moreover, the levels of peripheral serum LPS, IL-1, IL-6, and TNF-α were obviously decreased (P < 0.05). A change in the LPS level of superior mesenteric venous blood also revealed a dramatic decrease (P < 0.05). Additionally, RYGB resulted in a shift of cecal microbiota in obese rats with T2D.
Hypoglycemic effects after RYGB may be associated with improved levels of LPS, IL-1, IL-6, and TNF-α. Changes in the structure of cecal microbiota may also play an important role.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>31561205</pmid><doi>10.1016/j.nut.2019.110565</doi><tpages>1</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0899-9007 |
| ispartof | Nutrition (Burbank, Los Angeles County, Calif.), 2019-11, Vol.67-68, p.110565-110565, Article 110565 |
| issn | 0899-9007 1873-1244 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2299144658 |
| source | Elsevier ScienceDirect Journals Complete; ProQuest Central UK/Ireland |
| subjects | Bacteria Blood Body weight Cecal microbiota Cecum Deoxyribonucleic acid Diabetes Diabetes mellitus (non-insulin dependent) Diet DNA Enzyme-linked immunosorbent assay Experiments Fasting Gastric bypass Gastrointestinal surgery Gene expression Glucose Inflammation Inflammatory factors Insulin resistance Interleukin 1 Interleukin 6 Levels Lipopolysaccharide Lipopolysaccharides Medical research Metabolism Microbiota Obesity Probiotics Relative abundance Roux-en-Y gastric bypass rRNA Standardization Tumor necrosis factor-TNF Tumor necrosis factor-α Type 2 diabetes Veins & arteries Weight control |
| title | Changes of serum lipopolysaccharide, inflammatory factors, and cecal microbiota in obese rats with type 2 diabetes induced by Roux-en-Y gastric bypass |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T07%3A46%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Changes%20of%20serum%20lipopolysaccharide,%20inflammatory%20factors,%20and%20cecal%20microbiota%20in%20obese%20rats%20with%20type%202%20diabetes%20induced%20by%20Roux-en-Y%20gastric%20bypass&rft.jtitle=Nutrition%20(Burbank,%20Los%20Angeles%20County,%20Calif.)&rft.au=Wu,%20Jiao&rft.date=2019-11&rft.volume=67-68&rft.spage=110565&rft.epage=110565&rft.pages=110565-110565&rft.artnum=110565&rft.issn=0899-9007&rft.eissn=1873-1244&rft_id=info:doi/10.1016/j.nut.2019.110565&rft_dat=%3Cproquest_cross%3E2299144658%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2315932505&rft_id=info:pmid/31561205&rft_els_id=S0899900719301224&rfr_iscdi=true |