Toxoplasma gondii in invasive animals on the Island of Fernando de Noronha in Brazil: Molecular characterization and mouse virulence studies of new genotypes
[Display omitted] •Ten Toxoplasma gondii strains were isolated from black rats in the Island of Fernado de Noronha, Brazil.•Five genotypes were found, of which three are known and two are new.•Virulence studies revealed low and intermediate virulence of T. gondii strains from black rats.•Island of F...
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| creator | Costa Viegas de Lima, Débora de Melo, Renata Pimentel Bandeira Campos de Almeida, Jonatas Rodrigues Magalhães, Fernando Jorge Ribeiro Andrade, Muller de Morais Pedrosa, Camila Vasconcelos de Oliveira, Cynthia Andréa Nascimento Porto, Wagnner José Su, Chunlei Aparecido Mota, Rinaldo |
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•Ten Toxoplasma gondii strains were isolated from black rats in the Island of Fernado de Noronha, Brazil.•Five genotypes were found, of which three are known and two are new.•Virulence studies revealed low and intermediate virulence of T. gondii strains from black rats.•Island of Fernado de Noronha presents a high variability of Toxoplasma gondii strains in Black-rats.
This study aimed to genetically characterize and to determine virulence from Toxoplasma gondii samples from invasive animals in the Island of Fernando de Noronha, Brazil. Blood samples were collected from 21 tegu-lizard (Salvator merianae), 12 rock-cavies (Kerodon rupestris) and 154 black-rats (Rattus rattus) from the Island and MAT (cutoff 1:25) detected anti-T. gondii antibodies in 0% of the tegus (0/21); 58.3% of the rock-cavies (7/12) and 22.7% of rats (35/154). Tissue samples (brain, heart, liver and lung) from positive animals in MAT were collected for molecular analysis and for bioassay in Swiss Webster mice. After observation period, mice were euthanized, and serological detection and tissue cyst search in the brain were performed. The brain of positive animals for serological detection or tissue cyst search was cultured in MARC-145 cells for maintenance of the T. gondii isolate. No isolate was obtained from rock cavies. Nine isolates were obtained by bioassay of 35 seropositive black rats. DNA samples were extracted from rat tissues and from parasite isolates in cell culture, and genotyped using 10 PCR-RFLP markers. ToxoDB genotypes #78 (1) from rat tissue and #146 (4), #163 (2), #260 (2) and #291 (1) from cell culture were detected. Markers of genes ROP18 and ROP5 were analyzed and in vivo virulence test was conducted in mice. Analysis revealed two allele combinations, 3/1 and 3/3, indicating non-lethal T. gondii strains, which is supported by mouse virulence test. |
| doi_str_mv | 10.1016/j.cimid.2019.101347 |
| format | Article |
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•Ten Toxoplasma gondii strains were isolated from black rats in the Island of Fernado de Noronha, Brazil.•Five genotypes were found, of which three are known and two are new.•Virulence studies revealed low and intermediate virulence of T. gondii strains from black rats.•Island of Fernado de Noronha presents a high variability of Toxoplasma gondii strains in Black-rats.
This study aimed to genetically characterize and to determine virulence from Toxoplasma gondii samples from invasive animals in the Island of Fernando de Noronha, Brazil. Blood samples were collected from 21 tegu-lizard (Salvator merianae), 12 rock-cavies (Kerodon rupestris) and 154 black-rats (Rattus rattus) from the Island and MAT (cutoff 1:25) detected anti-T. gondii antibodies in 0% of the tegus (0/21); 58.3% of the rock-cavies (7/12) and 22.7% of rats (35/154). Tissue samples (brain, heart, liver and lung) from positive animals in MAT were collected for molecular analysis and for bioassay in Swiss Webster mice. After observation period, mice were euthanized, and serological detection and tissue cyst search in the brain were performed. The brain of positive animals for serological detection or tissue cyst search was cultured in MARC-145 cells for maintenance of the T. gondii isolate. No isolate was obtained from rock cavies. Nine isolates were obtained by bioassay of 35 seropositive black rats. DNA samples were extracted from rat tissues and from parasite isolates in cell culture, and genotyped using 10 PCR-RFLP markers. ToxoDB genotypes #78 (1) from rat tissue and #146 (4), #163 (2), #260 (2) and #291 (1) from cell culture were detected. Markers of genes ROP18 and ROP5 were analyzed and in vivo virulence test was conducted in mice. Analysis revealed two allele combinations, 3/1 and 3/3, indicating non-lethal T. gondii strains, which is supported by mouse virulence test.</description><identifier>ISSN: 0147-9571</identifier><identifier>EISSN: 1878-1667</identifier><identifier>DOI: 10.1016/j.cimid.2019.101347</identifier><identifier>PMID: 31546127</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animal tissues ; Animals ; Antibodies ; Antibodies, Protozoan - blood ; Bioassays ; Biomarkers ; Biotechnology ; Brain ; Brain - parasitology ; Brazil - epidemiology ; Cell culture ; Cell Line ; Cysts ; Deoxyribonucleic acid ; DNA ; DNA, Protozoan - genetics ; Genetic Variation - genetics ; Genotype ; Genotypes ; Heart - parasitology ; In vivo methods and tests ; Invasive animals ; Isolation ; Liver - parasitology ; Lizards - parasitology ; Lung - parasitology ; Mice ; Mn-PCR-RFLP ; Parasites ; Polymerase chain reaction ; Protozoa ; Rats ; Restriction fragment length polymorphism ; Rocks ; Rodents ; Toxoplasma - genetics ; Toxoplasma - immunology ; Toxoplasma - isolation & purification ; Toxoplasma gondii ; Toxoplasmosis ; Toxoplasmosis, Animal - epidemiology ; Virulence</subject><ispartof>Comparative immunology, microbiology and infectious diseases, 2019-12, Vol.67, p.101347-101347, Article 101347</ispartof><rights>2019 Elsevier Ltd</rights><rights>Copyright © 2019 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Science Ltd. Dec 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-ddbfbdb1831241bfab40a1ea150d1af761b38dadf0f9730de55b3099a13a83fe3</citedby><cites>FETCH-LOGICAL-c387t-ddbfbdb1831241bfab40a1ea150d1af761b38dadf0f9730de55b3099a13a83fe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cimid.2019.101347$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31546127$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Costa Viegas de Lima, Débora</creatorcontrib><creatorcontrib>de Melo, Renata Pimentel Bandeira</creatorcontrib><creatorcontrib>Campos de Almeida, Jonatas</creatorcontrib><creatorcontrib>Rodrigues Magalhães, Fernando Jorge</creatorcontrib><creatorcontrib>Ribeiro Andrade, Muller</creatorcontrib><creatorcontrib>de Morais Pedrosa, Camila</creatorcontrib><creatorcontrib>Vasconcelos de Oliveira, Cynthia Andréa</creatorcontrib><creatorcontrib>Nascimento Porto, Wagnner José</creatorcontrib><creatorcontrib>Su, Chunlei</creatorcontrib><creatorcontrib>Aparecido Mota, Rinaldo</creatorcontrib><title>Toxoplasma gondii in invasive animals on the Island of Fernando de Noronha in Brazil: Molecular characterization and mouse virulence studies of new genotypes</title><title>Comparative immunology, microbiology and infectious diseases</title><addtitle>Comp Immunol Microbiol Infect Dis</addtitle><description>[Display omitted]
•Ten Toxoplasma gondii strains were isolated from black rats in the Island of Fernado de Noronha, Brazil.•Five genotypes were found, of which three are known and two are new.•Virulence studies revealed low and intermediate virulence of T. gondii strains from black rats.•Island of Fernado de Noronha presents a high variability of Toxoplasma gondii strains in Black-rats.
This study aimed to genetically characterize and to determine virulence from Toxoplasma gondii samples from invasive animals in the Island of Fernando de Noronha, Brazil. Blood samples were collected from 21 tegu-lizard (Salvator merianae), 12 rock-cavies (Kerodon rupestris) and 154 black-rats (Rattus rattus) from the Island and MAT (cutoff 1:25) detected anti-T. gondii antibodies in 0% of the tegus (0/21); 58.3% of the rock-cavies (7/12) and 22.7% of rats (35/154). Tissue samples (brain, heart, liver and lung) from positive animals in MAT were collected for molecular analysis and for bioassay in Swiss Webster mice. After observation period, mice were euthanized, and serological detection and tissue cyst search in the brain were performed. The brain of positive animals for serological detection or tissue cyst search was cultured in MARC-145 cells for maintenance of the T. gondii isolate. No isolate was obtained from rock cavies. Nine isolates were obtained by bioassay of 35 seropositive black rats. DNA samples were extracted from rat tissues and from parasite isolates in cell culture, and genotyped using 10 PCR-RFLP markers. ToxoDB genotypes #78 (1) from rat tissue and #146 (4), #163 (2), #260 (2) and #291 (1) from cell culture were detected. Markers of genes ROP18 and ROP5 were analyzed and in vivo virulence test was conducted in mice. Analysis revealed two allele combinations, 3/1 and 3/3, indicating non-lethal T. gondii strains, which is supported by mouse virulence test.</description><subject>Animal tissues</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Protozoan - blood</subject><subject>Bioassays</subject><subject>Biomarkers</subject><subject>Biotechnology</subject><subject>Brain</subject><subject>Brain - parasitology</subject><subject>Brazil - epidemiology</subject><subject>Cell culture</subject><subject>Cell Line</subject><subject>Cysts</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Protozoan - genetics</subject><subject>Genetic Variation - genetics</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Heart - parasitology</subject><subject>In vivo methods and tests</subject><subject>Invasive animals</subject><subject>Isolation</subject><subject>Liver - parasitology</subject><subject>Lizards - parasitology</subject><subject>Lung - parasitology</subject><subject>Mice</subject><subject>Mn-PCR-RFLP</subject><subject>Parasites</subject><subject>Polymerase chain reaction</subject><subject>Protozoa</subject><subject>Rats</subject><subject>Restriction fragment length polymorphism</subject><subject>Rocks</subject><subject>Rodents</subject><subject>Toxoplasma - genetics</subject><subject>Toxoplasma - immunology</subject><subject>Toxoplasma - isolation & purification</subject><subject>Toxoplasma gondii</subject><subject>Toxoplasmosis</subject><subject>Toxoplasmosis, Animal - epidemiology</subject><subject>Virulence</subject><issn>0147-9571</issn><issn>1878-1667</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAURS1ERYfCFyAhS2zYZOoXJ3GMxKJUFCoV2JS15dgvHY8Se7CTgfZf-FccpmXRBZIlW9a599n3EvIK2BoYNKfbtXGjs-uSgVxueCWekBW0oi2gacRTsmJQiULWAo7J85S2jDEJFTwjxxzqqoFSrMjv6_Ar7AadRk1vgrfOUefz2uvk9ki1d6MeEg2eThukl2nQ3tLQ0wuMPh8DtUi_hhj8Ri_CD1HfueEd_RIGNPOgIzUbHbWZMLo7PbnssxiMYU5I9y7OA3qDNE2zdZgWY48_6Q36MN3uML0gR30ejy_v9xPy_eLj9fnn4urbp8vzs6vC8FZMhbVd39kOWg5lBV2vu4ppQA01s6B70UDHW6ttz3opOLNY1x1nUmrguuU98hPy9uC7i-HHjGlSo0sGh_xbzE9VZSmbpil5U2X0zSN0G-acxZApXspSSC4hU_xAmRhSitirXcxJxlsFTC3tqa36255a2lOH9rLq9b333I1o_2ke6srA-wOAOYy9w6iScUuC1kU0k7LB_XfAH9VUrxY</recordid><startdate>201912</startdate><enddate>201912</enddate><creator>Costa Viegas de Lima, Débora</creator><creator>de Melo, Renata Pimentel Bandeira</creator><creator>Campos de Almeida, Jonatas</creator><creator>Rodrigues Magalhães, Fernando Jorge</creator><creator>Ribeiro Andrade, Muller</creator><creator>de Morais Pedrosa, Camila</creator><creator>Vasconcelos de Oliveira, Cynthia Andréa</creator><creator>Nascimento Porto, Wagnner José</creator><creator>Su, Chunlei</creator><creator>Aparecido Mota, Rinaldo</creator><general>Elsevier Ltd</general><general>Elsevier Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>201912</creationdate><title>Toxoplasma gondii in invasive animals on the Island of Fernando de Noronha in Brazil: Molecular characterization and mouse virulence studies of new genotypes</title><author>Costa Viegas de Lima, Débora ; de Melo, Renata Pimentel Bandeira ; Campos de Almeida, Jonatas ; Rodrigues Magalhães, Fernando Jorge ; Ribeiro Andrade, Muller ; de Morais Pedrosa, Camila ; Vasconcelos de Oliveira, Cynthia Andréa ; Nascimento Porto, Wagnner José ; Su, Chunlei ; Aparecido Mota, Rinaldo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-ddbfbdb1831241bfab40a1ea150d1af761b38dadf0f9730de55b3099a13a83fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animal tissues</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Protozoan - blood</topic><topic>Bioassays</topic><topic>Biomarkers</topic><topic>Biotechnology</topic><topic>Brain</topic><topic>Brain - parasitology</topic><topic>Brazil - epidemiology</topic><topic>Cell culture</topic><topic>Cell Line</topic><topic>Cysts</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Protozoan - genetics</topic><topic>Genetic Variation - genetics</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>Heart - parasitology</topic><topic>In vivo methods and tests</topic><topic>Invasive animals</topic><topic>Isolation</topic><topic>Liver - parasitology</topic><topic>Lizards - parasitology</topic><topic>Lung - parasitology</topic><topic>Mice</topic><topic>Mn-PCR-RFLP</topic><topic>Parasites</topic><topic>Polymerase chain reaction</topic><topic>Protozoa</topic><topic>Rats</topic><topic>Restriction fragment length polymorphism</topic><topic>Rocks</topic><topic>Rodents</topic><topic>Toxoplasma - genetics</topic><topic>Toxoplasma - immunology</topic><topic>Toxoplasma - isolation & purification</topic><topic>Toxoplasma gondii</topic><topic>Toxoplasmosis</topic><topic>Toxoplasmosis, Animal - epidemiology</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Costa Viegas de Lima, Débora</creatorcontrib><creatorcontrib>de Melo, Renata Pimentel Bandeira</creatorcontrib><creatorcontrib>Campos de Almeida, Jonatas</creatorcontrib><creatorcontrib>Rodrigues Magalhães, Fernando Jorge</creatorcontrib><creatorcontrib>Ribeiro Andrade, Muller</creatorcontrib><creatorcontrib>de Morais Pedrosa, Camila</creatorcontrib><creatorcontrib>Vasconcelos de Oliveira, Cynthia Andréa</creatorcontrib><creatorcontrib>Nascimento Porto, Wagnner José</creatorcontrib><creatorcontrib>Su, Chunlei</creatorcontrib><creatorcontrib>Aparecido Mota, Rinaldo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Comparative immunology, microbiology and infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Costa Viegas de Lima, Débora</au><au>de Melo, Renata Pimentel Bandeira</au><au>Campos de Almeida, Jonatas</au><au>Rodrigues Magalhães, Fernando Jorge</au><au>Ribeiro Andrade, Muller</au><au>de Morais Pedrosa, Camila</au><au>Vasconcelos de Oliveira, Cynthia Andréa</au><au>Nascimento Porto, Wagnner José</au><au>Su, Chunlei</au><au>Aparecido Mota, Rinaldo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Toxoplasma gondii in invasive animals on the Island of Fernando de Noronha in Brazil: Molecular characterization and mouse virulence studies of new genotypes</atitle><jtitle>Comparative immunology, microbiology and infectious diseases</jtitle><addtitle>Comp Immunol Microbiol Infect Dis</addtitle><date>2019-12</date><risdate>2019</risdate><volume>67</volume><spage>101347</spage><epage>101347</epage><pages>101347-101347</pages><artnum>101347</artnum><issn>0147-9571</issn><eissn>1878-1667</eissn><abstract>[Display omitted]
•Ten Toxoplasma gondii strains were isolated from black rats in the Island of Fernado de Noronha, Brazil.•Five genotypes were found, of which three are known and two are new.•Virulence studies revealed low and intermediate virulence of T. gondii strains from black rats.•Island of Fernado de Noronha presents a high variability of Toxoplasma gondii strains in Black-rats.
This study aimed to genetically characterize and to determine virulence from Toxoplasma gondii samples from invasive animals in the Island of Fernando de Noronha, Brazil. Blood samples were collected from 21 tegu-lizard (Salvator merianae), 12 rock-cavies (Kerodon rupestris) and 154 black-rats (Rattus rattus) from the Island and MAT (cutoff 1:25) detected anti-T. gondii antibodies in 0% of the tegus (0/21); 58.3% of the rock-cavies (7/12) and 22.7% of rats (35/154). Tissue samples (brain, heart, liver and lung) from positive animals in MAT were collected for molecular analysis and for bioassay in Swiss Webster mice. After observation period, mice were euthanized, and serological detection and tissue cyst search in the brain were performed. The brain of positive animals for serological detection or tissue cyst search was cultured in MARC-145 cells for maintenance of the T. gondii isolate. No isolate was obtained from rock cavies. Nine isolates were obtained by bioassay of 35 seropositive black rats. DNA samples were extracted from rat tissues and from parasite isolates in cell culture, and genotyped using 10 PCR-RFLP markers. ToxoDB genotypes #78 (1) from rat tissue and #146 (4), #163 (2), #260 (2) and #291 (1) from cell culture were detected. Markers of genes ROP18 and ROP5 were analyzed and in vivo virulence test was conducted in mice. Analysis revealed two allele combinations, 3/1 and 3/3, indicating non-lethal T. gondii strains, which is supported by mouse virulence test.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>31546127</pmid><doi>10.1016/j.cimid.2019.101347</doi><tpages>1</tpages></addata></record> |
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| ispartof | Comparative immunology, microbiology and infectious diseases, 2019-12, Vol.67, p.101347-101347, Article 101347 |
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| subjects | Animal tissues Animals Antibodies Antibodies, Protozoan - blood Bioassays Biomarkers Biotechnology Brain Brain - parasitology Brazil - epidemiology Cell culture Cell Line Cysts Deoxyribonucleic acid DNA DNA, Protozoan - genetics Genetic Variation - genetics Genotype Genotypes Heart - parasitology In vivo methods and tests Invasive animals Isolation Liver - parasitology Lizards - parasitology Lung - parasitology Mice Mn-PCR-RFLP Parasites Polymerase chain reaction Protozoa Rats Restriction fragment length polymorphism Rocks Rodents Toxoplasma - genetics Toxoplasma - immunology Toxoplasma - isolation & purification Toxoplasma gondii Toxoplasmosis Toxoplasmosis, Animal - epidemiology Virulence |
| title | Toxoplasma gondii in invasive animals on the Island of Fernando de Noronha in Brazil: Molecular characterization and mouse virulence studies of new genotypes |
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