3D observation of chromosome scaffold structure using a 360° electron tomography sample holder

•New method of transferring a chromosome to a 3D electron tomography holder.•Application of the method to chromosomes does not induce any visible damage.•Condensin is likely organized in helical structure of the chromosome scaffold. The chromosome scaffold is considered to be a key structure of the...

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Veröffentlicht in:	Micron (Oxford, England : 1993) England : 1993), 2019-11, Vol.126, p.102736-102736, Article 102736
Hauptverfasser:	Phengchat, Rinyaporn, Hayashida, Misa, Ohmido, Nobuko, Homeniuk, Darren, Fukui, Kiichi
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromosomal Proteins, Non-Histone - chemistry Chromosome scaffold Chromosomes - ultrastructure Condensin Electron Microscope Tomography - methods Electron tomography Gold HeLa Cells Humans Immuno-electroscopy Metal Nanoparticles Metaphase Microscopy, Immunoelectron - methods Specimen Handling
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