Characterization of virulence factors, antifungal resistance with ERG-11 gene among Candida species isolated from pulmonary samples
Candida is a part of the normal oropharyngeal flora and the upper respiratory tract. Candida albicans(C. albicans), is the predominant species causing respiratory tract infections associated with pneumonia. Resistance to azole antifungal agents among the C. albicans may be due to alteration of the t...
Gespeichert in:
| Veröffentlicht in: | Microbial pathogenesis 2019-12, Vol.137, p.103750-103750, Article 103750 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 103750 |
|---|---|
| container_issue | |
| container_start_page | 103750 |
| container_title | Microbial pathogenesis |
| container_volume | 137 |
| creator | Danis Vijay, D. Jayanthi, S. Meenakshi, N. Meharaj, S.H. Shifa Sujhithra, A. Perumal, J. |
| description | Candida is a part of the normal oropharyngeal flora and the upper respiratory tract. Candida albicans(C. albicans), is the predominant species causing respiratory tract infections associated with pneumonia. Resistance to azole antifungal agents among the C. albicans may be due to alteration of the target enzymes, which are encoded in ERG11 gene. The biofilm formation may also be a cause to antifungal resistance.
This study was conducted at Chettinad Hospital and Research Institute. Samples were collected from June 2018–June 2019, for a period of 1 year. After species confirmation, virulence factor among the Candida species were identified by hemolysis test, coagulase test and biofilm formation. Genotypic confirmation of C. albicans and their azole resistance due to ERG 11 gene were done using multiplex PCR.
In our study, 31 (55%) C. albicans, 8 (14%) Candida glabrata(C. glabrata) and 10 (17%) Candida tropicalis(C. tropicalis), three Aspergillus flavus(A. flavus), two Aspergillus fumigatous (A. fumigatous), one Aspergillus niger (A. niger) and one Mucor species were isolated. In C. albicans, 31 were positive for Germ tube and Chalmydospore formation. Six of candida species were isolated along with bacterial co infection. Among the Candida isolates, 17 (55%) C. albicans strains were strongly biofilm positive and 14(45%) were negative. The susceptibility pattern of (n = 31) C. albicans were as follows: fluconazole (21(68%) S, 10(32%) R), voriconazole (22(71%)S),9(21%) R) and Amphotericin B 31(100%) S). Among the 19 C. albicans, four were positive for ERG11 gene.
The isolation of C. albicans and non - albicans from respiratory specimens should be reconsidered as these organisms are re-emerging pathogens. Speciation is needed due to variation in species pathogenicity and their susceptibility.
•Multiplex PCR in identification of fungal pathogens.•Early diagnosis and treatment reduces the burden in health care settings with better prognosis.•This study has focused on ERG11 gene associated with resistant isolates among colonizer and pathogens. |
| doi_str_mv | 10.1016/j.micpath.2019.103750 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2295470741</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0882401019314184</els_id><sourcerecordid>2295470741</sourcerecordid><originalsourceid>FETCH-LOGICAL-c365t-571206e50481fcf0f7aee1ec5f9e3776fd7cc91fdb5f5a8457a28030e724dedc3</originalsourceid><addsrcrecordid>eNqFkE2P0zAQQC0EYsvCTwD5yIGUcRLHyQmhalmQVkJCcLZm7XHrKomD7SyCK38cVy1cOY008-brMfZSwFaA6N4et5M3C-bDtgYxlFyjJDxiGwFDV4ka-sdsA31fVy0IuGLPUjoCwNA2w1N21QjZdD2IDfu9O2BEkyn6X5h9mHlw_MHHdaTZEHelFGJ6w3HO3q3zHkceKfmU8VT-4fOB33y5rYTge5qJ4xTmPd_hbL1FnhYynhL3KYyYyXIXw8SXdSwUxp884bSMlJ6zJw7HRC8u8Zp9-3Dzdfexuvt8-2n3_q4yTSdzJVV5qyMJbS-cceAUEgky0g3UKNU5q4wZhLP30knsW6mw7qEBUnVryZrmmr0-z11i-L5SynryydA44kxhTbquB9kqUK0oqDyjJoaUIjm9RD-Vm7UAffKvj_riX5_867P_0vfqsmK9n8j-6_orvADvzgCVRx88RZ2KouLS-kgmaxv8f1b8ASABm74</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2295470741</pqid></control><display><type>article</type><title>Characterization of virulence factors, antifungal resistance with ERG-11 gene among Candida species isolated from pulmonary samples</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Danis Vijay, D. ; Jayanthi, S. ; Meenakshi, N. ; Meharaj, S.H. Shifa ; Sujhithra, A. ; Perumal, J.</creator><creatorcontrib>Danis Vijay, D. ; Jayanthi, S. ; Meenakshi, N. ; Meharaj, S.H. Shifa ; Sujhithra, A. ; Perumal, J.</creatorcontrib><description>Candida is a part of the normal oropharyngeal flora and the upper respiratory tract. Candida albicans(C. albicans), is the predominant species causing respiratory tract infections associated with pneumonia. Resistance to azole antifungal agents among the C. albicans may be due to alteration of the target enzymes, which are encoded in ERG11 gene. The biofilm formation may also be a cause to antifungal resistance.
This study was conducted at Chettinad Hospital and Research Institute. Samples were collected from June 2018–June 2019, for a period of 1 year. After species confirmation, virulence factor among the Candida species were identified by hemolysis test, coagulase test and biofilm formation. Genotypic confirmation of C. albicans and their azole resistance due to ERG 11 gene were done using multiplex PCR.
In our study, 31 (55%) C. albicans, 8 (14%) Candida glabrata(C. glabrata) and 10 (17%) Candida tropicalis(C. tropicalis), three Aspergillus flavus(A. flavus), two Aspergillus fumigatous (A. fumigatous), one Aspergillus niger (A. niger) and one Mucor species were isolated. In C. albicans, 31 were positive for Germ tube and Chalmydospore formation. Six of candida species were isolated along with bacterial co infection. Among the Candida isolates, 17 (55%) C. albicans strains were strongly biofilm positive and 14(45%) were negative. The susceptibility pattern of (n = 31) C. albicans were as follows: fluconazole (21(68%) S, 10(32%) R), voriconazole (22(71%)S),9(21%) R) and Amphotericin B 31(100%) S). Among the 19 C. albicans, four were positive for ERG11 gene.
The isolation of C. albicans and non - albicans from respiratory specimens should be reconsidered as these organisms are re-emerging pathogens. Speciation is needed due to variation in species pathogenicity and their susceptibility.
•Multiplex PCR in identification of fungal pathogens.•Early diagnosis and treatment reduces the burden in health care settings with better prognosis.•This study has focused on ERG11 gene associated with resistant isolates among colonizer and pathogens.</description><identifier>ISSN: 0882-4010</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1016/j.micpath.2019.103750</identifier><identifier>PMID: 31536801</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; Amphotericin B - pharmacology ; Antifungal Agents - pharmacology ; Aspergillus - drug effects ; Azole resistance ; Bacteria - isolation & purification ; Biofilm ; Biofilms - growth & development ; Candida - drug effects ; Candida - genetics ; Candida - isolation & purification ; Candida albicans ; Candida albicans - drug effects ; Coagulase - analysis ; Drug Resistance, Fungal - drug effects ; Drug Resistance, Fungal - genetics ; ERG11 gene ; Fluconazole - pharmacology ; Genotype ; Humans ; Microbial Sensitivity Tests ; Middle Aged ; Respiratory tract infections ; Transcriptional Regulator ERG - genetics ; Virulence Factors - genetics ; Voriconazole - pharmacology ; Young Adult</subject><ispartof>Microbial pathogenesis, 2019-12, Vol.137, p.103750-103750, Article 103750</ispartof><rights>2019 Elsevier Ltd</rights><rights>Copyright © 2019 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-571206e50481fcf0f7aee1ec5f9e3776fd7cc91fdb5f5a8457a28030e724dedc3</citedby><cites>FETCH-LOGICAL-c365t-571206e50481fcf0f7aee1ec5f9e3776fd7cc91fdb5f5a8457a28030e724dedc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0882401019314184$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31536801$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Danis Vijay, D.</creatorcontrib><creatorcontrib>Jayanthi, S.</creatorcontrib><creatorcontrib>Meenakshi, N.</creatorcontrib><creatorcontrib>Meharaj, S.H. Shifa</creatorcontrib><creatorcontrib>Sujhithra, A.</creatorcontrib><creatorcontrib>Perumal, J.</creatorcontrib><title>Characterization of virulence factors, antifungal resistance with ERG-11 gene among Candida species isolated from pulmonary samples</title><title>Microbial pathogenesis</title><addtitle>Microb Pathog</addtitle><description>Candida is a part of the normal oropharyngeal flora and the upper respiratory tract. Candida albicans(C. albicans), is the predominant species causing respiratory tract infections associated with pneumonia. Resistance to azole antifungal agents among the C. albicans may be due to alteration of the target enzymes, which are encoded in ERG11 gene. The biofilm formation may also be a cause to antifungal resistance.
This study was conducted at Chettinad Hospital and Research Institute. Samples were collected from June 2018–June 2019, for a period of 1 year. After species confirmation, virulence factor among the Candida species were identified by hemolysis test, coagulase test and biofilm formation. Genotypic confirmation of C. albicans and their azole resistance due to ERG 11 gene were done using multiplex PCR.
In our study, 31 (55%) C. albicans, 8 (14%) Candida glabrata(C. glabrata) and 10 (17%) Candida tropicalis(C. tropicalis), three Aspergillus flavus(A. flavus), two Aspergillus fumigatous (A. fumigatous), one Aspergillus niger (A. niger) and one Mucor species were isolated. In C. albicans, 31 were positive for Germ tube and Chalmydospore formation. Six of candida species were isolated along with bacterial co infection. Among the Candida isolates, 17 (55%) C. albicans strains were strongly biofilm positive and 14(45%) were negative. The susceptibility pattern of (n = 31) C. albicans were as follows: fluconazole (21(68%) S, 10(32%) R), voriconazole (22(71%)S),9(21%) R) and Amphotericin B 31(100%) S). Among the 19 C. albicans, four were positive for ERG11 gene.
The isolation of C. albicans and non - albicans from respiratory specimens should be reconsidered as these organisms are re-emerging pathogens. Speciation is needed due to variation in species pathogenicity and their susceptibility.
•Multiplex PCR in identification of fungal pathogens.•Early diagnosis and treatment reduces the burden in health care settings with better prognosis.•This study has focused on ERG11 gene associated with resistant isolates among colonizer and pathogens.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Amphotericin B - pharmacology</subject><subject>Antifungal Agents - pharmacology</subject><subject>Aspergillus - drug effects</subject><subject>Azole resistance</subject><subject>Bacteria - isolation & purification</subject><subject>Biofilm</subject><subject>Biofilms - growth & development</subject><subject>Candida - drug effects</subject><subject>Candida - genetics</subject><subject>Candida - isolation & purification</subject><subject>Candida albicans</subject><subject>Candida albicans - drug effects</subject><subject>Coagulase - analysis</subject><subject>Drug Resistance, Fungal - drug effects</subject><subject>Drug Resistance, Fungal - genetics</subject><subject>ERG11 gene</subject><subject>Fluconazole - pharmacology</subject><subject>Genotype</subject><subject>Humans</subject><subject>Microbial Sensitivity Tests</subject><subject>Middle Aged</subject><subject>Respiratory tract infections</subject><subject>Transcriptional Regulator ERG - genetics</subject><subject>Virulence Factors - genetics</subject><subject>Voriconazole - pharmacology</subject><subject>Young Adult</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2P0zAQQC0EYsvCTwD5yIGUcRLHyQmhalmQVkJCcLZm7XHrKomD7SyCK38cVy1cOY008-brMfZSwFaA6N4et5M3C-bDtgYxlFyjJDxiGwFDV4ka-sdsA31fVy0IuGLPUjoCwNA2w1N21QjZdD2IDfu9O2BEkyn6X5h9mHlw_MHHdaTZEHelFGJ6w3HO3q3zHkceKfmU8VT-4fOB33y5rYTge5qJ4xTmPd_hbL1FnhYynhL3KYyYyXIXw8SXdSwUxp884bSMlJ6zJw7HRC8u8Zp9-3Dzdfexuvt8-2n3_q4yTSdzJVV5qyMJbS-cceAUEgky0g3UKNU5q4wZhLP30knsW6mw7qEBUnVryZrmmr0-z11i-L5SynryydA44kxhTbquB9kqUK0oqDyjJoaUIjm9RD-Vm7UAffKvj_riX5_867P_0vfqsmK9n8j-6_orvADvzgCVRx88RZ2KouLS-kgmaxv8f1b8ASABm74</recordid><startdate>201912</startdate><enddate>201912</enddate><creator>Danis Vijay, D.</creator><creator>Jayanthi, S.</creator><creator>Meenakshi, N.</creator><creator>Meharaj, S.H. Shifa</creator><creator>Sujhithra, A.</creator><creator>Perumal, J.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201912</creationdate><title>Characterization of virulence factors, antifungal resistance with ERG-11 gene among Candida species isolated from pulmonary samples</title><author>Danis Vijay, D. ; Jayanthi, S. ; Meenakshi, N. ; Meharaj, S.H. Shifa ; Sujhithra, A. ; Perumal, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-571206e50481fcf0f7aee1ec5f9e3776fd7cc91fdb5f5a8457a28030e724dedc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Amphotericin B - pharmacology</topic><topic>Antifungal Agents - pharmacology</topic><topic>Aspergillus - drug effects</topic><topic>Azole resistance</topic><topic>Bacteria - isolation & purification</topic><topic>Biofilm</topic><topic>Biofilms - growth & development</topic><topic>Candida - drug effects</topic><topic>Candida - genetics</topic><topic>Candida - isolation & purification</topic><topic>Candida albicans</topic><topic>Candida albicans - drug effects</topic><topic>Coagulase - analysis</topic><topic>Drug Resistance, Fungal - drug effects</topic><topic>Drug Resistance, Fungal - genetics</topic><topic>ERG11 gene</topic><topic>Fluconazole - pharmacology</topic><topic>Genotype</topic><topic>Humans</topic><topic>Microbial Sensitivity Tests</topic><topic>Middle Aged</topic><topic>Respiratory tract infections</topic><topic>Transcriptional Regulator ERG - genetics</topic><topic>Virulence Factors - genetics</topic><topic>Voriconazole - pharmacology</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Danis Vijay, D.</creatorcontrib><creatorcontrib>Jayanthi, S.</creatorcontrib><creatorcontrib>Meenakshi, N.</creatorcontrib><creatorcontrib>Meharaj, S.H. Shifa</creatorcontrib><creatorcontrib>Sujhithra, A.</creatorcontrib><creatorcontrib>Perumal, J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Danis Vijay, D.</au><au>Jayanthi, S.</au><au>Meenakshi, N.</au><au>Meharaj, S.H. Shifa</au><au>Sujhithra, A.</au><au>Perumal, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of virulence factors, antifungal resistance with ERG-11 gene among Candida species isolated from pulmonary samples</atitle><jtitle>Microbial pathogenesis</jtitle><addtitle>Microb Pathog</addtitle><date>2019-12</date><risdate>2019</risdate><volume>137</volume><spage>103750</spage><epage>103750</epage><pages>103750-103750</pages><artnum>103750</artnum><issn>0882-4010</issn><eissn>1096-1208</eissn><abstract>Candida is a part of the normal oropharyngeal flora and the upper respiratory tract. Candida albicans(C. albicans), is the predominant species causing respiratory tract infections associated with pneumonia. Resistance to azole antifungal agents among the C. albicans may be due to alteration of the target enzymes, which are encoded in ERG11 gene. The biofilm formation may also be a cause to antifungal resistance.
This study was conducted at Chettinad Hospital and Research Institute. Samples were collected from June 2018–June 2019, for a period of 1 year. After species confirmation, virulence factor among the Candida species were identified by hemolysis test, coagulase test and biofilm formation. Genotypic confirmation of C. albicans and their azole resistance due to ERG 11 gene were done using multiplex PCR.
In our study, 31 (55%) C. albicans, 8 (14%) Candida glabrata(C. glabrata) and 10 (17%) Candida tropicalis(C. tropicalis), three Aspergillus flavus(A. flavus), two Aspergillus fumigatous (A. fumigatous), one Aspergillus niger (A. niger) and one Mucor species were isolated. In C. albicans, 31 were positive for Germ tube and Chalmydospore formation. Six of candida species were isolated along with bacterial co infection. Among the Candida isolates, 17 (55%) C. albicans strains were strongly biofilm positive and 14(45%) were negative. The susceptibility pattern of (n = 31) C. albicans were as follows: fluconazole (21(68%) S, 10(32%) R), voriconazole (22(71%)S),9(21%) R) and Amphotericin B 31(100%) S). Among the 19 C. albicans, four were positive for ERG11 gene.
The isolation of C. albicans and non - albicans from respiratory specimens should be reconsidered as these organisms are re-emerging pathogens. Speciation is needed due to variation in species pathogenicity and their susceptibility.
•Multiplex PCR in identification of fungal pathogens.•Early diagnosis and treatment reduces the burden in health care settings with better prognosis.•This study has focused on ERG11 gene associated with resistant isolates among colonizer and pathogens.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>31536801</pmid><doi>10.1016/j.micpath.2019.103750</doi><tpages>1</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0882-4010 |
| ispartof | Microbial pathogenesis, 2019-12, Vol.137, p.103750-103750, Article 103750 |
| issn | 0882-4010 1096-1208 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2295470741 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adolescent Adult Aged Aged, 80 and over Amphotericin B - pharmacology Antifungal Agents - pharmacology Aspergillus - drug effects Azole resistance Bacteria - isolation & purification Biofilm Biofilms - growth & development Candida - drug effects Candida - genetics Candida - isolation & purification Candida albicans Candida albicans - drug effects Coagulase - analysis Drug Resistance, Fungal - drug effects Drug Resistance, Fungal - genetics ERG11 gene Fluconazole - pharmacology Genotype Humans Microbial Sensitivity Tests Middle Aged Respiratory tract infections Transcriptional Regulator ERG - genetics Virulence Factors - genetics Voriconazole - pharmacology Young Adult |
| title | Characterization of virulence factors, antifungal resistance with ERG-11 gene among Candida species isolated from pulmonary samples |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T00%3A38%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20virulence%20factors,%20antifungal%20resistance%20with%20ERG-11%20gene%20among%20Candida%20species%20isolated%20from%20pulmonary%20samples&rft.jtitle=Microbial%20pathogenesis&rft.au=Danis%20Vijay,%20D.&rft.date=2019-12&rft.volume=137&rft.spage=103750&rft.epage=103750&rft.pages=103750-103750&rft.artnum=103750&rft.issn=0882-4010&rft.eissn=1096-1208&rft_id=info:doi/10.1016/j.micpath.2019.103750&rft_dat=%3Cproquest_cross%3E2295470741%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2295470741&rft_id=info:pmid/31536801&rft_els_id=S0882401019314184&rfr_iscdi=true |