Oocyte maturation in plasma or follicular fluid obtained from lipopolysaccharide-treated cows disrupts its developmental competence
Mastitis has deleterious effects on ovarian function and reproductive performance. We studied the association between plasma or follicular fluid (FF) obtained from endotoxin-induced mastitic cows, and oocyte developmental competence. Lactating Holstein cows were synchronized using the Ovsynch protoc...
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| Veröffentlicht in: | Theriogenology 2020-01, Vol.141, p.120-127 |
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| creator | Roth, Zvi Dvir, Ayala Furman, Ori Lavon, Yaniv Kalo, Dorit Leitner, Gabriel Wolfenson, David |
| description | Mastitis has deleterious effects on ovarian function and reproductive performance. We studied the association between plasma or follicular fluid (FF) obtained from endotoxin-induced mastitic cows, and oocyte developmental competence. Lactating Holstein cows were synchronized using the Ovsynch protocol. On Day 6 of the synchronized cycle, an additional PGF2α dose was administered, and either Escherichia coli endotoxin (LPS, 10 μg; n = 3 cows) or saline (n = 3 cows) was administered to one udder quarter per cow, 36 h later. Milk samples were collected and rectal temperatures recorded. Cows treated with LPS showed a typical transient increase in body temperature (40.3 °C ± 0.4), whereas cows treated with saline maintained normal body temperature (38.9 °C ± 0.04). A higher (P |
| doi_str_mv | 10.1016/j.theriogenology.2019.09.021 |
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•The study associates between plasma or follicular fluid obtained from endotoxin-induced mastitic cows, and oocyte developmental competence.•LPS increased body temperature, somatic cell count in the milk; reduced cortisol and reduced estradiol concentration in the plasma.•Maturation of bovine oocytes in follicular fluid or plasma obtained from LPS-mastitic cows, reduced their developmental competence.</description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/j.theriogenology.2019.09.021</identifier><identifier>PMID: 31536861</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cattle ; Culture Media ; Developmental competence ; DNA Fragmentation ; Female ; Follicular Fluid - chemistry ; In Vitro Oocyte Maturation Techniques - veterinary ; Lipopolysaccharide ; Lipopolysaccharides - toxicity ; Mastitis ; Mastitis, Bovine - chemically induced ; Oocyte maturation ; Oocytes - drug effects ; Plasma</subject><ispartof>Theriogenology, 2020-01, Vol.141, p.120-127</ispartof><rights>2019 Elsevier Inc.</rights><rights>Copyright © 2019 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-44a26290a7d2ea44f015ed4fe15eaf831c05241c72b0e8c616b456038896e6f53</citedby><cites>FETCH-LOGICAL-c386t-44a26290a7d2ea44f015ed4fe15eaf831c05241c72b0e8c616b456038896e6f53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0093691X1930411X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31536861$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Roth, Zvi</creatorcontrib><creatorcontrib>Dvir, Ayala</creatorcontrib><creatorcontrib>Furman, Ori</creatorcontrib><creatorcontrib>Lavon, Yaniv</creatorcontrib><creatorcontrib>Kalo, Dorit</creatorcontrib><creatorcontrib>Leitner, Gabriel</creatorcontrib><creatorcontrib>Wolfenson, David</creatorcontrib><title>Oocyte maturation in plasma or follicular fluid obtained from lipopolysaccharide-treated cows disrupts its developmental competence</title><title>Theriogenology</title><addtitle>Theriogenology</addtitle><description>Mastitis has deleterious effects on ovarian function and reproductive performance. We studied the association between plasma or follicular fluid (FF) obtained from endotoxin-induced mastitic cows, and oocyte developmental competence. Lactating Holstein cows were synchronized using the Ovsynch protocol. On Day 6 of the synchronized cycle, an additional PGF2α dose was administered, and either Escherichia coli endotoxin (LPS, 10 μg; n = 3 cows) or saline (n = 3 cows) was administered to one udder quarter per cow, 36 h later. Milk samples were collected and rectal temperatures recorded. Cows treated with LPS showed a typical transient increase in body temperature (40.3 °C ± 0.4), whereas cows treated with saline maintained normal body temperature (38.9 °C ± 0.04). A higher (P < 0.05) somatic cell count was recorded for cows treated with LPS. Plasma samples were collected and FF was aspirated from the preovulatory follicles by transvaginal ultrasound probe, 6 h after LPS administration. Radioimmunoassay was performed on plasma samples to determine estradiol and cortisol concentrations. Either FF or plasma was further used as maturation medium. In the first experiment, oocytes were matured in TCM-199 (Control) or in FF aspirated from cows treated with saline (FF-Saline) or LPS (FF-LPS). Cleavage rate to the 2- to 4-cell stage embryo did not differ among groups. However, the proportion of developed blastocysts on Day 7 postfertilization in the FF-LPS group tended to be lower for that in FF-Saline and was lower (P < 0.05) than that in the Control groups (10.6 vs. 22.4 and 24.4%, respectively). In the second experiment, oocytes were matured in TCM-199 (Control), or in plasma obtained from cows treated with saline (Plasma-Saline) or LPS (Plasma-LPS). Similar to the FF findings, cleavage rate did not differ among groups; however, the proportion of developing blastocysts tended to be lower in the Plasma-LPS group than in the Plasma-Saline group and was lower (P < 0.05) from that in the Control group (11.0 vs. 25.5 and 34.7%, respectively). The proportion of apoptotic cells per blastocyst, determined by TUNEL assay, did not differ among the experimental groups. The findings shed light on the mechanism by which mastitis induces a disruption in oocyte developmental competence. Further studies are required to clarify whether the negative effect on oocyte developmental competence is a result of LPS, by itself, or due to elevation of secondary inflammatory agents.
•The study associates between plasma or follicular fluid obtained from endotoxin-induced mastitic cows, and oocyte developmental competence.•LPS increased body temperature, somatic cell count in the milk; reduced cortisol and reduced estradiol concentration in the plasma.•Maturation of bovine oocytes in follicular fluid or plasma obtained from LPS-mastitic cows, reduced their developmental competence.</description><subject>Animals</subject><subject>Cattle</subject><subject>Culture Media</subject><subject>Developmental competence</subject><subject>DNA Fragmentation</subject><subject>Female</subject><subject>Follicular Fluid - chemistry</subject><subject>In Vitro Oocyte Maturation Techniques - veterinary</subject><subject>Lipopolysaccharide</subject><subject>Lipopolysaccharides - toxicity</subject><subject>Mastitis</subject><subject>Mastitis, Bovine - chemically induced</subject><subject>Oocyte maturation</subject><subject>Oocytes - drug effects</subject><subject>Plasma</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUcGKFDEQDaK44-ovSA4evPRYSboz3eBFFleFhb0oeAuZpHo3Q7qrTdIrc_bHzTCr4E2o4hW8V1VUPcbeCNgKEPrdYVvuMQW6w5ki3R23EsSwhRpSPGEb0e-GRkklnrINwKAaPYjvF-xFzgcAUFqL5-xCiU7pXosN-3VL7liQT7asyZZAMw8zX6LNk-WU-EgxBrdGW8u4Bs9pX2yY0fMx0cRjWGiheMzWuXubgsemJLSl8o5-Zu5DTutSMg81PT5gpGXCudhY-WnBgrPDl-zZaGPGV494yb5df_x69bm5uf305erDTeNUr0vTtlZqOYDdeYm2bUcQHfp2xAp27JVw0MlWuJ3cA_ZOC71vOw2q7weNeuzUJXt7nrsk-rFiLmYK2WGMdkZas5Fy6NodaNBV-v4sdYlyTjiaJYXJpqMRYE42mIP51wZzssFADSlq--vHTet-Qv-3-c_fq-D6LMB670PAZLILp1_4kNAV4yn836bfceymCQ</recordid><startdate>20200101</startdate><enddate>20200101</enddate><creator>Roth, Zvi</creator><creator>Dvir, Ayala</creator><creator>Furman, Ori</creator><creator>Lavon, Yaniv</creator><creator>Kalo, Dorit</creator><creator>Leitner, Gabriel</creator><creator>Wolfenson, David</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20200101</creationdate><title>Oocyte maturation in plasma or follicular fluid obtained from lipopolysaccharide-treated cows disrupts its developmental competence</title><author>Roth, Zvi ; Dvir, Ayala ; Furman, Ori ; Lavon, Yaniv ; Kalo, Dorit ; Leitner, Gabriel ; Wolfenson, David</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-44a26290a7d2ea44f015ed4fe15eaf831c05241c72b0e8c616b456038896e6f53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Cattle</topic><topic>Culture Media</topic><topic>Developmental competence</topic><topic>DNA Fragmentation</topic><topic>Female</topic><topic>Follicular Fluid - chemistry</topic><topic>In Vitro Oocyte Maturation Techniques - veterinary</topic><topic>Lipopolysaccharide</topic><topic>Lipopolysaccharides - toxicity</topic><topic>Mastitis</topic><topic>Mastitis, Bovine - chemically induced</topic><topic>Oocyte maturation</topic><topic>Oocytes - drug effects</topic><topic>Plasma</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roth, Zvi</creatorcontrib><creatorcontrib>Dvir, Ayala</creatorcontrib><creatorcontrib>Furman, Ori</creatorcontrib><creatorcontrib>Lavon, Yaniv</creatorcontrib><creatorcontrib>Kalo, Dorit</creatorcontrib><creatorcontrib>Leitner, Gabriel</creatorcontrib><creatorcontrib>Wolfenson, David</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roth, Zvi</au><au>Dvir, Ayala</au><au>Furman, Ori</au><au>Lavon, Yaniv</au><au>Kalo, Dorit</au><au>Leitner, Gabriel</au><au>Wolfenson, David</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Oocyte maturation in plasma or follicular fluid obtained from lipopolysaccharide-treated cows disrupts its developmental competence</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>2020-01-01</date><risdate>2020</risdate><volume>141</volume><spage>120</spage><epage>127</epage><pages>120-127</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract>Mastitis has deleterious effects on ovarian function and reproductive performance. We studied the association between plasma or follicular fluid (FF) obtained from endotoxin-induced mastitic cows, and oocyte developmental competence. Lactating Holstein cows were synchronized using the Ovsynch protocol. On Day 6 of the synchronized cycle, an additional PGF2α dose was administered, and either Escherichia coli endotoxin (LPS, 10 μg; n = 3 cows) or saline (n = 3 cows) was administered to one udder quarter per cow, 36 h later. Milk samples were collected and rectal temperatures recorded. Cows treated with LPS showed a typical transient increase in body temperature (40.3 °C ± 0.4), whereas cows treated with saline maintained normal body temperature (38.9 °C ± 0.04). A higher (P < 0.05) somatic cell count was recorded for cows treated with LPS. Plasma samples were collected and FF was aspirated from the preovulatory follicles by transvaginal ultrasound probe, 6 h after LPS administration. Radioimmunoassay was performed on plasma samples to determine estradiol and cortisol concentrations. Either FF or plasma was further used as maturation medium. In the first experiment, oocytes were matured in TCM-199 (Control) or in FF aspirated from cows treated with saline (FF-Saline) or LPS (FF-LPS). Cleavage rate to the 2- to 4-cell stage embryo did not differ among groups. However, the proportion of developed blastocysts on Day 7 postfertilization in the FF-LPS group tended to be lower for that in FF-Saline and was lower (P < 0.05) than that in the Control groups (10.6 vs. 22.4 and 24.4%, respectively). In the second experiment, oocytes were matured in TCM-199 (Control), or in plasma obtained from cows treated with saline (Plasma-Saline) or LPS (Plasma-LPS). Similar to the FF findings, cleavage rate did not differ among groups; however, the proportion of developing blastocysts tended to be lower in the Plasma-LPS group than in the Plasma-Saline group and was lower (P < 0.05) from that in the Control group (11.0 vs. 25.5 and 34.7%, respectively). The proportion of apoptotic cells per blastocyst, determined by TUNEL assay, did not differ among the experimental groups. The findings shed light on the mechanism by which mastitis induces a disruption in oocyte developmental competence. Further studies are required to clarify whether the negative effect on oocyte developmental competence is a result of LPS, by itself, or due to elevation of secondary inflammatory agents.
•The study associates between plasma or follicular fluid obtained from endotoxin-induced mastitic cows, and oocyte developmental competence.•LPS increased body temperature, somatic cell count in the milk; reduced cortisol and reduced estradiol concentration in the plasma.•Maturation of bovine oocytes in follicular fluid or plasma obtained from LPS-mastitic cows, reduced their developmental competence.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>31536861</pmid><doi>10.1016/j.theriogenology.2019.09.021</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Cattle Culture Media Developmental competence DNA Fragmentation Female Follicular Fluid - chemistry In Vitro Oocyte Maturation Techniques - veterinary Lipopolysaccharide Lipopolysaccharides - toxicity Mastitis Mastitis, Bovine - chemically induced Oocyte maturation Oocytes - drug effects Plasma |
| title | Oocyte maturation in plasma or follicular fluid obtained from lipopolysaccharide-treated cows disrupts its developmental competence |
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