Revival of Krebs–Ringer balanced salt solution for the investigation of polymorphonuclear leukocytes and Pseudomonas aeruginosa biofilm interaction

ABSTRACT To study the interaction between aggregating bacteria and polymorphonuclear leukocytes (PMNs) in vitro, the chosen medium must favor both the isolated PMNs and the bacteria. To investigate the best-suited medium for the in vitro survival of isolated unactivated human PMNs, we compared three...

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Veröffentlicht in:	Pathogens and Disease 2019-07, Vol.77 (5), p.1
Hauptverfasser:	Bjarnsholt, Thomas, Jensen, Peter Østrup, Alhede, Maria
Format:	Artikel
Sprache:	eng
Schlagworte:	Agglomeration Arginine Bacteria Bacterial diseases Bacterial infections Biofilms Biofilms - growth & development Cells, Cultured Comparative analysis Culture media (Biology) Cytological Techniques Granulocytes Growth Histone H3 Histones Host-Pathogen Interactions Humans L-Lactate dehydrogenase Lactate dehydrogenase Lactic acid Leukocytes Leukocytes (polymorphonuclear) Lysine Microbial mats Microbiological Techniques Neutrophils - immunology Neutrophils - microbiology Properties Pseudomonas aeruginosa Pseudomonas aeruginosa - growth & development Pseudomonas aeruginosa - immunology Saline solutions Solutions - chemistry Survival
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