LncRNA RHPN1-AS1 promoted cell proliferation, invasion and migration in cervical cancer via the modulation of miR-299–3p/FGF2 axis
This study aims to determine the biological function and underlying mechanisms of lncRNA RHPN1 antisense RNA1 (RHPN1-AS1) in cervical cancer cell proliferation, invasion and migration. Gene expression was analysed by quantitative real-time PCR; protein levels were determined by western blot assay; i...
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| Veröffentlicht in: | Life sciences (1973) 2019-12, Vol.239, p.116856-116856, Article 116856 |
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| creator | Duan, Haixia Li, Xiaojuan Chen, Youyi Wang, Yan Li, Zhibin |
| description | This study aims to determine the biological function and underlying mechanisms of lncRNA RHPN1 antisense RNA1 (RHPN1-AS1) in cervical cancer cell proliferation, invasion and migration.
Gene expression was analysed by quantitative real-time PCR; protein levels were determined by western blot assay; in vitro functional assays determined the cervical cancer cell progression; in vivo tumor growth of cervical cancer cell was determined in nude mice xenograft models.
The results showed that RHPN1-AS1 was up-regulated in cervical cancer tissues and cell lines. In vitro functional assays demonstrated that RHPN1-AS1 overexpression promoted SiHa cell proliferation, invasion and migration; while RHPN1-AS1 knockdown showed the opposite effects. In vivo study showed that RHPN1-AS1 knockdown suppressed tumor growth in the nude mice. Further investigation showed that miR-299–3p was targeted and inversely regulated by RHPN1-AS1. In addition, miR-299–3p targeted the 3’ untranslated region of fibroblast growth factor 2 (FGF2) to suppress its expression. The rescue experiments showed that the enhanced effects of RHPN1-AS1 overexpression on cell proliferation, growth, invasion and migration in SiHa cells were significantly attenuated by miR-299–3p overexpression or FGF2 inhibition. On the other hand, knockdown of miR-299–3p and overexpression of FGF2 both significantly increased cell proliferation, growth, invasion and migration in SiHa cells transfected with RHPN1-AS1 siRNA.
In conclusion, our results revealed that RHPN1-AS1 promoted cervical cancer progression via targeting miR-299–3p/FGF2 axis. Our data suggested that RHPN1-AS1/miR-299–3p/FGF2 axis may be a promising target for cervical cancer treatment. |
| doi_str_mv | 10.1016/j.lfs.2019.116856 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2292091834</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0024320519307830</els_id><sourcerecordid>2292091834</sourcerecordid><originalsourceid>FETCH-LOGICAL-c381t-8b35af5eb4c4fbec7e325743f3227842dc32a4052b0e860cfd73ef6bdf4be473</originalsourceid><addsrcrecordid>eNp9kU1uFDEQhS1ERIbAAdggS2xY0BP_9o9YjSImQRoFNGRvue0yeNTdHuzuEeyyyA1yw5wEjzphwYKVy6XvPVXVQ-gNJUtKaHm-W3YuLRmhzZLSspblM7SgddUUpOT0OVoQwkTBGZGn6GVKO0KIlBV_gU45lUwK1izQ3WYw2-sV3l59vabF6hvF-xj6MILFBrru-Ou8g6hHH4YP2A8HnXKF9WBx77_P_dzOdDx4ozts9JBrfPAajz8A98FO3UwFlyXbgjXNw-0935-vL9cM618-vUInTncJXj--Z-hm_enm4qrYfLn8fLHaFIbXdCzqlkvtJLTCCNeCqYAzWQnuOGNVLZg1nGlBJGsJ1CUxzlYcXNlaJ1oQFT9D72fbvNTPCdKoep-OW-oBwpQUYw0jDa25yOi7f9BdmOKQh1OMc96IkhOZKTpTJoaUIji1j77X8beiRB0TUjuVE1LHhNScUNa8fXSe2h7sX8VTJBn4OAOQL3HwEFUyHvJNrY9gRmWD_4_9H9yNoI4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2333946305</pqid></control><display><type>article</type><title>LncRNA RHPN1-AS1 promoted cell proliferation, invasion and migration in cervical cancer via the modulation of miR-299–3p/FGF2 axis</title><source>Elsevier ScienceDirect Journals Complete - AutoHoldings</source><source>MEDLINE</source><creator>Duan, Haixia ; Li, Xiaojuan ; Chen, Youyi ; Wang, Yan ; Li, Zhibin</creator><creatorcontrib>Duan, Haixia ; Li, Xiaojuan ; Chen, Youyi ; Wang, Yan ; Li, Zhibin</creatorcontrib><description>This study aims to determine the biological function and underlying mechanisms of lncRNA RHPN1 antisense RNA1 (RHPN1-AS1) in cervical cancer cell proliferation, invasion and migration.
Gene expression was analysed by quantitative real-time PCR; protein levels were determined by western blot assay; in vitro functional assays determined the cervical cancer cell progression; in vivo tumor growth of cervical cancer cell was determined in nude mice xenograft models.
The results showed that RHPN1-AS1 was up-regulated in cervical cancer tissues and cell lines. In vitro functional assays demonstrated that RHPN1-AS1 overexpression promoted SiHa cell proliferation, invasion and migration; while RHPN1-AS1 knockdown showed the opposite effects. In vivo study showed that RHPN1-AS1 knockdown suppressed tumor growth in the nude mice. Further investigation showed that miR-299–3p was targeted and inversely regulated by RHPN1-AS1. In addition, miR-299–3p targeted the 3’ untranslated region of fibroblast growth factor 2 (FGF2) to suppress its expression. The rescue experiments showed that the enhanced effects of RHPN1-AS1 overexpression on cell proliferation, growth, invasion and migration in SiHa cells were significantly attenuated by miR-299–3p overexpression or FGF2 inhibition. On the other hand, knockdown of miR-299–3p and overexpression of FGF2 both significantly increased cell proliferation, growth, invasion and migration in SiHa cells transfected with RHPN1-AS1 siRNA.
In conclusion, our results revealed that RHPN1-AS1 promoted cervical cancer progression via targeting miR-299–3p/FGF2 axis. Our data suggested that RHPN1-AS1/miR-299–3p/FGF2 axis may be a promising target for cervical cancer treatment.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/j.lfs.2019.116856</identifier><identifier>PMID: 31525429</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>3' Untranslated Regions ; Animal models ; Animals ; Antisense RNA ; Assaying ; Cancer ; Cell growth ; Cell Line, Tumor ; Cell migration ; Cell Movement - genetics ; Cell proliferation ; Cell Proliferation - genetics ; Cervical cancer ; Cervix ; Female ; FGF2 ; Fibroblast growth factor 2 ; Fibroblast Growth Factor 2 - metabolism ; Gene expression ; Growth factors ; Heterografts ; Humans ; In vivo methods and tests ; Invasion and migration ; Kinases ; lncRNA RHPN1-AS1 ; Mice ; Mice, Nude ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miR-299–3p ; Neoplasm Invasiveness ; RNA, Long Noncoding - genetics ; RNA, Long Noncoding - metabolism ; RNA, Small Interfering - genetics ; RNA, Small Interfering - metabolism ; siRNA ; Tumor cell lines ; Tumors ; Xenografts ; Xenotransplantation</subject><ispartof>Life sciences (1973), 2019-12, Vol.239, p.116856-116856, Article 116856</ispartof><rights>2019 Elsevier Inc.</rights><rights>Copyright © 2019 Elsevier Inc. All rights reserved.</rights><rights>Copyright Elsevier BV Dec 15, 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-8b35af5eb4c4fbec7e325743f3227842dc32a4052b0e860cfd73ef6bdf4be473</citedby><cites>FETCH-LOGICAL-c381t-8b35af5eb4c4fbec7e325743f3227842dc32a4052b0e860cfd73ef6bdf4be473</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.lfs.2019.116856$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31525429$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Duan, Haixia</creatorcontrib><creatorcontrib>Li, Xiaojuan</creatorcontrib><creatorcontrib>Chen, Youyi</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Li, Zhibin</creatorcontrib><title>LncRNA RHPN1-AS1 promoted cell proliferation, invasion and migration in cervical cancer via the modulation of miR-299–3p/FGF2 axis</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>This study aims to determine the biological function and underlying mechanisms of lncRNA RHPN1 antisense RNA1 (RHPN1-AS1) in cervical cancer cell proliferation, invasion and migration.
Gene expression was analysed by quantitative real-time PCR; protein levels were determined by western blot assay; in vitro functional assays determined the cervical cancer cell progression; in vivo tumor growth of cervical cancer cell was determined in nude mice xenograft models.
The results showed that RHPN1-AS1 was up-regulated in cervical cancer tissues and cell lines. In vitro functional assays demonstrated that RHPN1-AS1 overexpression promoted SiHa cell proliferation, invasion and migration; while RHPN1-AS1 knockdown showed the opposite effects. In vivo study showed that RHPN1-AS1 knockdown suppressed tumor growth in the nude mice. Further investigation showed that miR-299–3p was targeted and inversely regulated by RHPN1-AS1. In addition, miR-299–3p targeted the 3’ untranslated region of fibroblast growth factor 2 (FGF2) to suppress its expression. The rescue experiments showed that the enhanced effects of RHPN1-AS1 overexpression on cell proliferation, growth, invasion and migration in SiHa cells were significantly attenuated by miR-299–3p overexpression or FGF2 inhibition. On the other hand, knockdown of miR-299–3p and overexpression of FGF2 both significantly increased cell proliferation, growth, invasion and migration in SiHa cells transfected with RHPN1-AS1 siRNA.
In conclusion, our results revealed that RHPN1-AS1 promoted cervical cancer progression via targeting miR-299–3p/FGF2 axis. Our data suggested that RHPN1-AS1/miR-299–3p/FGF2 axis may be a promising target for cervical cancer treatment.</description><subject>3' Untranslated Regions</subject><subject>Animal models</subject><subject>Animals</subject><subject>Antisense RNA</subject><subject>Assaying</subject><subject>Cancer</subject><subject>Cell growth</subject><subject>Cell Line, Tumor</subject><subject>Cell migration</subject><subject>Cell Movement - genetics</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - genetics</subject><subject>Cervical cancer</subject><subject>Cervix</subject><subject>Female</subject><subject>FGF2</subject><subject>Fibroblast growth factor 2</subject><subject>Fibroblast Growth Factor 2 - metabolism</subject><subject>Gene expression</subject><subject>Growth factors</subject><subject>Heterografts</subject><subject>Humans</subject><subject>In vivo methods and tests</subject><subject>Invasion and migration</subject><subject>Kinases</subject><subject>lncRNA RHPN1-AS1</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>miR-299–3p</subject><subject>Neoplasm Invasiveness</subject><subject>RNA, Long Noncoding - genetics</subject><subject>RNA, Long Noncoding - metabolism</subject><subject>RNA, Small Interfering - genetics</subject><subject>RNA, Small Interfering - metabolism</subject><subject>siRNA</subject><subject>Tumor cell lines</subject><subject>Tumors</subject><subject>Xenografts</subject><subject>Xenotransplantation</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1uFDEQhS1ERIbAAdggS2xY0BP_9o9YjSImQRoFNGRvue0yeNTdHuzuEeyyyA1yw5wEjzphwYKVy6XvPVXVQ-gNJUtKaHm-W3YuLRmhzZLSspblM7SgddUUpOT0OVoQwkTBGZGn6GVKO0KIlBV_gU45lUwK1izQ3WYw2-sV3l59vabF6hvF-xj6MILFBrru-Ou8g6hHH4YP2A8HnXKF9WBx77_P_dzOdDx4ozts9JBrfPAajz8A98FO3UwFlyXbgjXNw-0935-vL9cM618-vUInTncJXj--Z-hm_enm4qrYfLn8fLHaFIbXdCzqlkvtJLTCCNeCqYAzWQnuOGNVLZg1nGlBJGsJ1CUxzlYcXNlaJ1oQFT9D72fbvNTPCdKoep-OW-oBwpQUYw0jDa25yOi7f9BdmOKQh1OMc96IkhOZKTpTJoaUIji1j77X8beiRB0TUjuVE1LHhNScUNa8fXSe2h7sX8VTJBn4OAOQL3HwEFUyHvJNrY9gRmWD_4_9H9yNoI4</recordid><startdate>20191215</startdate><enddate>20191215</enddate><creator>Duan, Haixia</creator><creator>Li, Xiaojuan</creator><creator>Chen, Youyi</creator><creator>Wang, Yan</creator><creator>Li, Zhibin</creator><general>Elsevier Inc</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20191215</creationdate><title>LncRNA RHPN1-AS1 promoted cell proliferation, invasion and migration in cervical cancer via the modulation of miR-299–3p/FGF2 axis</title><author>Duan, Haixia ; Li, Xiaojuan ; Chen, Youyi ; Wang, Yan ; Li, Zhibin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-8b35af5eb4c4fbec7e325743f3227842dc32a4052b0e860cfd73ef6bdf4be473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>3' Untranslated Regions</topic><topic>Animal models</topic><topic>Animals</topic><topic>Antisense RNA</topic><topic>Assaying</topic><topic>Cancer</topic><topic>Cell growth</topic><topic>Cell Line, Tumor</topic><topic>Cell migration</topic><topic>Cell Movement - genetics</topic><topic>Cell proliferation</topic><topic>Cell Proliferation - genetics</topic><topic>Cervical cancer</topic><topic>Cervix</topic><topic>Female</topic><topic>FGF2</topic><topic>Fibroblast growth factor 2</topic><topic>Fibroblast Growth Factor 2 - metabolism</topic><topic>Gene expression</topic><topic>Growth factors</topic><topic>Heterografts</topic><topic>Humans</topic><topic>In vivo methods and tests</topic><topic>Invasion and migration</topic><topic>Kinases</topic><topic>lncRNA RHPN1-AS1</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>miR-299–3p</topic><topic>Neoplasm Invasiveness</topic><topic>RNA, Long Noncoding - genetics</topic><topic>RNA, Long Noncoding - metabolism</topic><topic>RNA, Small Interfering - genetics</topic><topic>RNA, Small Interfering - metabolism</topic><topic>siRNA</topic><topic>Tumor cell lines</topic><topic>Tumors</topic><topic>Xenografts</topic><topic>Xenotransplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Duan, Haixia</creatorcontrib><creatorcontrib>Li, Xiaojuan</creatorcontrib><creatorcontrib>Chen, Youyi</creatorcontrib><creatorcontrib>Wang, Yan</creatorcontrib><creatorcontrib>Li, Zhibin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Duan, Haixia</au><au>Li, Xiaojuan</au><au>Chen, Youyi</au><au>Wang, Yan</au><au>Li, Zhibin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>LncRNA RHPN1-AS1 promoted cell proliferation, invasion and migration in cervical cancer via the modulation of miR-299–3p/FGF2 axis</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2019-12-15</date><risdate>2019</risdate><volume>239</volume><spage>116856</spage><epage>116856</epage><pages>116856-116856</pages><artnum>116856</artnum><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>This study aims to determine the biological function and underlying mechanisms of lncRNA RHPN1 antisense RNA1 (RHPN1-AS1) in cervical cancer cell proliferation, invasion and migration.
Gene expression was analysed by quantitative real-time PCR; protein levels were determined by western blot assay; in vitro functional assays determined the cervical cancer cell progression; in vivo tumor growth of cervical cancer cell was determined in nude mice xenograft models.
The results showed that RHPN1-AS1 was up-regulated in cervical cancer tissues and cell lines. In vitro functional assays demonstrated that RHPN1-AS1 overexpression promoted SiHa cell proliferation, invasion and migration; while RHPN1-AS1 knockdown showed the opposite effects. In vivo study showed that RHPN1-AS1 knockdown suppressed tumor growth in the nude mice. Further investigation showed that miR-299–3p was targeted and inversely regulated by RHPN1-AS1. In addition, miR-299–3p targeted the 3’ untranslated region of fibroblast growth factor 2 (FGF2) to suppress its expression. The rescue experiments showed that the enhanced effects of RHPN1-AS1 overexpression on cell proliferation, growth, invasion and migration in SiHa cells were significantly attenuated by miR-299–3p overexpression or FGF2 inhibition. On the other hand, knockdown of miR-299–3p and overexpression of FGF2 both significantly increased cell proliferation, growth, invasion and migration in SiHa cells transfected with RHPN1-AS1 siRNA.
In conclusion, our results revealed that RHPN1-AS1 promoted cervical cancer progression via targeting miR-299–3p/FGF2 axis. Our data suggested that RHPN1-AS1/miR-299–3p/FGF2 axis may be a promising target for cervical cancer treatment.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>31525429</pmid><doi>10.1016/j.lfs.2019.116856</doi><tpages>1</tpages></addata></record> |
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| subjects | 3' Untranslated Regions Animal models Animals Antisense RNA Assaying Cancer Cell growth Cell Line, Tumor Cell migration Cell Movement - genetics Cell proliferation Cell Proliferation - genetics Cervical cancer Cervix Female FGF2 Fibroblast growth factor 2 Fibroblast Growth Factor 2 - metabolism Gene expression Growth factors Heterografts Humans In vivo methods and tests Invasion and migration Kinases lncRNA RHPN1-AS1 Mice Mice, Nude MicroRNAs - genetics MicroRNAs - metabolism miR-299–3p Neoplasm Invasiveness RNA, Long Noncoding - genetics RNA, Long Noncoding - metabolism RNA, Small Interfering - genetics RNA, Small Interfering - metabolism siRNA Tumor cell lines Tumors Xenografts Xenotransplantation |
| title | LncRNA RHPN1-AS1 promoted cell proliferation, invasion and migration in cervical cancer via the modulation of miR-299–3p/FGF2 axis |
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