Effects of melatonin on morphology and development of primordial follicles during in vitro culture of bovine ovarian tissue
This study aims to investigate the effect of melatonin on activation, growth and morphology of bovine primordial follicles, as well as on stromal cells density in ovarian tissues after in vitro culture. Ovarian fragments were cultured in α‐MEM+ alone or supplemented with melatonin (250, 500, 1,000 o...
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| Veröffentlicht in: | Reproduction in domestic animals 2019-12, Vol.54 (12), p.1567-1573 |
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| creator | Cavalcante, Barbara N. Matos‐Brito, Bruno G. Paulino, Lais R. F. M. Silva, Bianca R. Aguiar, Antonio Wesley Melo Almeida, Edmar Felipe Maia Souza, Ana Liza Paz Vasconcelos, Gisvani Lopes De Assis, Ernando Igo Teixeira Silva, Anderson W. B. Silva, José Roberto V. |
| description | This study aims to investigate the effect of melatonin on activation, growth and morphology of bovine primordial follicles, as well as on stromal cells density in ovarian tissues after in vitro culture. Ovarian fragments were cultured in α‐MEM+ alone or supplemented with melatonin (250, 500, 1,000 or 2,000 pM) for a period of six days. Non‐cultured and cultured tissues were processed for histological analysis; according to developmental stages, follicles were classified as primordial or growing follicles. These follicles were further classified as morphologically normal or degenerated. Ovarian stromal cell density was also evaluated. The percentages of primordial and developing follicles, as well as those classified of normal follicles, were compared by Fisher's exact test, and the differences were considered significant when p |
| doi_str_mv | 10.1111/rda.13565 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2290901997</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2290901997</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3535-7a955c6127f75c41e9fc8ae14347e38b0d608b3f5ba0ab3594ad2e610a53c1403</originalsourceid><addsrcrecordid>eNp10U1PHCEcBnBi2tRVe_ALGJJe7GEUhgGGo1H7kpg0aeqZMMwfi2FghZk1m3552a7toUm5wOHHw8uD0CklF7SOyzyaC8q44AdoRTumGsIZfYNWRDHRCCn6Q3RUyiMhlPdSvkOHjPKWcCFX6Netc2DngpPDEwQzp-gjThFPKa9_ppAettjEEY-wgZDWE8R5R9fZVzB6E7BLIXgboOBxyT4-4Lp_4-ecsF3CvGTY-SFtfKyrjcneRDz7UhY4QW-dCQXev87H6P7T7Y_rL83dt89fr6_uGss44400inMraCud5LajoJztDdSHdhJYP5BRkH5gjg-GmIFx1ZmxBUGJ4czSjrBjdL7PXef0tECZ9eSLhRBMhLQU3baKKEKVkpV--Ic-piXHejvdsurqmaKv6uNe2ZxKyeD07j9M3mpK9K4RXRvRvxup9uw1cRkmGP_KPxVUcLkHzz7A9v9J-vvN1T7yBaa-ljE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2322943468</pqid></control><display><type>article</type><title>Effects of melatonin on morphology and development of primordial follicles during in vitro culture of bovine ovarian tissue</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Cavalcante, Barbara N. ; Matos‐Brito, Bruno G. ; Paulino, Lais R. F. M. ; Silva, Bianca R. ; Aguiar, Antonio Wesley Melo ; Almeida, Edmar Felipe Maia ; Souza, Ana Liza Paz ; Vasconcelos, Gisvani Lopes ; De Assis, Ernando Igo Teixeira ; Silva, Anderson W. B. ; Silva, José Roberto V.</creator><creatorcontrib>Cavalcante, Barbara N. ; Matos‐Brito, Bruno G. ; Paulino, Lais R. F. M. ; Silva, Bianca R. ; Aguiar, Antonio Wesley Melo ; Almeida, Edmar Felipe Maia ; Souza, Ana Liza Paz ; Vasconcelos, Gisvani Lopes ; De Assis, Ernando Igo Teixeira ; Silva, Anderson W. B. ; Silva, José Roberto V.</creatorcontrib><description>This study aims to investigate the effect of melatonin on activation, growth and morphology of bovine primordial follicles, as well as on stromal cells density in ovarian tissues after in vitro culture. Ovarian fragments were cultured in α‐MEM+ alone or supplemented with melatonin (250, 500, 1,000 or 2,000 pM) for a period of six days. Non‐cultured and cultured tissues were processed for histological analysis; according to developmental stages, follicles were classified as primordial or growing follicles. These follicles were further classified as morphologically normal or degenerated. Ovarian stromal cell density was also evaluated. The percentages of primordial and developing follicles, as well as those classified of normal follicles, were compared by Fisher's exact test, and the differences were considered significant when p < .05. The results showed that the presence of 1,000 and 2,000 pM melatonin in culture medium promoted a reduction in the percentage of primordial follicles and an increase in the percentage of development follicles, when compared to follicles cultured in control medium. On the other hand, the presence of 250 or 500 pM melatonin did not show a significant effect on the percentage of primordial and developing follicles. Besides that, the presence of 500, 1,000 and 2,000 pM melatonin maintained the percentage of normal follicles similar to those seen uncultured control. Moreover, tissues cultured in presence of 1,000 pM melatonin showed a higher percentage of normal follicles when compared to follicles cultured in the presence of 250 pM melatonin. It was observed a similar profile of stromal density in both uncultured tissues and those cultured in vitro in the presence of melatonin. In conclusion, melatonin (1,000 and 2,000 pM) promotes bovine primordial follicles activation and maintains the stromal cell density during in vitro culture of ovarian cortical tissue.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/rda.13565</identifier><identifier>PMID: 31520567</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Activation ; Animals ; antioxidant ; Antioxidants - pharmacology ; Cattle ; Cell culture ; Cell density ; Cortex ; Cytology ; Density ; Developmental stages ; Female ; Follicles ; follicular development ; Melatonin ; Melatonin - pharmacology ; Morphology ; Ovarian Follicle - drug effects ; Ovarian Follicle - growth & development ; Stromal cells ; Tissue culture ; Tissue Culture Techniques - veterinary ; Tissues</subject><ispartof>Reproduction in domestic animals, 2019-12, Vol.54 (12), p.1567-1573</ispartof><rights>2019 Blackwell Verlag GmbH</rights><rights>2019 Blackwell Verlag GmbH.</rights><rights>Copyright © 2019 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3535-7a955c6127f75c41e9fc8ae14347e38b0d608b3f5ba0ab3594ad2e610a53c1403</citedby><cites>FETCH-LOGICAL-c3535-7a955c6127f75c41e9fc8ae14347e38b0d608b3f5ba0ab3594ad2e610a53c1403</cites><orcidid>0000-0002-5970-6177</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Frda.13565$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Frda.13565$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31520567$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cavalcante, Barbara N.</creatorcontrib><creatorcontrib>Matos‐Brito, Bruno G.</creatorcontrib><creatorcontrib>Paulino, Lais R. F. M.</creatorcontrib><creatorcontrib>Silva, Bianca R.</creatorcontrib><creatorcontrib>Aguiar, Antonio Wesley Melo</creatorcontrib><creatorcontrib>Almeida, Edmar Felipe Maia</creatorcontrib><creatorcontrib>Souza, Ana Liza Paz</creatorcontrib><creatorcontrib>Vasconcelos, Gisvani Lopes</creatorcontrib><creatorcontrib>De Assis, Ernando Igo Teixeira</creatorcontrib><creatorcontrib>Silva, Anderson W. B.</creatorcontrib><creatorcontrib>Silva, José Roberto V.</creatorcontrib><title>Effects of melatonin on morphology and development of primordial follicles during in vitro culture of bovine ovarian tissue</title><title>Reproduction in domestic animals</title><addtitle>Reprod Domest Anim</addtitle><description>This study aims to investigate the effect of melatonin on activation, growth and morphology of bovine primordial follicles, as well as on stromal cells density in ovarian tissues after in vitro culture. Ovarian fragments were cultured in α‐MEM+ alone or supplemented with melatonin (250, 500, 1,000 or 2,000 pM) for a period of six days. Non‐cultured and cultured tissues were processed for histological analysis; according to developmental stages, follicles were classified as primordial or growing follicles. These follicles were further classified as morphologically normal or degenerated. Ovarian stromal cell density was also evaluated. The percentages of primordial and developing follicles, as well as those classified of normal follicles, were compared by Fisher's exact test, and the differences were considered significant when p < .05. The results showed that the presence of 1,000 and 2,000 pM melatonin in culture medium promoted a reduction in the percentage of primordial follicles and an increase in the percentage of development follicles, when compared to follicles cultured in control medium. On the other hand, the presence of 250 or 500 pM melatonin did not show a significant effect on the percentage of primordial and developing follicles. Besides that, the presence of 500, 1,000 and 2,000 pM melatonin maintained the percentage of normal follicles similar to those seen uncultured control. Moreover, tissues cultured in presence of 1,000 pM melatonin showed a higher percentage of normal follicles when compared to follicles cultured in the presence of 250 pM melatonin. It was observed a similar profile of stromal density in both uncultured tissues and those cultured in vitro in the presence of melatonin. In conclusion, melatonin (1,000 and 2,000 pM) promotes bovine primordial follicles activation and maintains the stromal cell density during in vitro culture of ovarian cortical tissue.</description><subject>Activation</subject><subject>Animals</subject><subject>antioxidant</subject><subject>Antioxidants - pharmacology</subject><subject>Cattle</subject><subject>Cell culture</subject><subject>Cell density</subject><subject>Cortex</subject><subject>Cytology</subject><subject>Density</subject><subject>Developmental stages</subject><subject>Female</subject><subject>Follicles</subject><subject>follicular development</subject><subject>Melatonin</subject><subject>Melatonin - pharmacology</subject><subject>Morphology</subject><subject>Ovarian Follicle - drug effects</subject><subject>Ovarian Follicle - growth & development</subject><subject>Stromal cells</subject><subject>Tissue culture</subject><subject>Tissue Culture Techniques - veterinary</subject><subject>Tissues</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10U1PHCEcBnBi2tRVe_ALGJJe7GEUhgGGo1H7kpg0aeqZMMwfi2FghZk1m3552a7toUm5wOHHw8uD0CklF7SOyzyaC8q44AdoRTumGsIZfYNWRDHRCCn6Q3RUyiMhlPdSvkOHjPKWcCFX6Netc2DngpPDEwQzp-gjThFPKa9_ppAettjEEY-wgZDWE8R5R9fZVzB6E7BLIXgboOBxyT4-4Lp_4-ecsF3CvGTY-SFtfKyrjcneRDz7UhY4QW-dCQXev87H6P7T7Y_rL83dt89fr6_uGss44400inMraCud5LajoJztDdSHdhJYP5BRkH5gjg-GmIFx1ZmxBUGJ4czSjrBjdL7PXef0tECZ9eSLhRBMhLQU3baKKEKVkpV--Ic-piXHejvdsurqmaKv6uNe2ZxKyeD07j9M3mpK9K4RXRvRvxup9uw1cRkmGP_KPxVUcLkHzz7A9v9J-vvN1T7yBaa-ljE</recordid><startdate>201912</startdate><enddate>201912</enddate><creator>Cavalcante, Barbara N.</creator><creator>Matos‐Brito, Bruno G.</creator><creator>Paulino, Lais R. F. M.</creator><creator>Silva, Bianca R.</creator><creator>Aguiar, Antonio Wesley Melo</creator><creator>Almeida, Edmar Felipe Maia</creator><creator>Souza, Ana Liza Paz</creator><creator>Vasconcelos, Gisvani Lopes</creator><creator>De Assis, Ernando Igo Teixeira</creator><creator>Silva, Anderson W. B.</creator><creator>Silva, José Roberto V.</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5970-6177</orcidid></search><sort><creationdate>201912</creationdate><title>Effects of melatonin on morphology and development of primordial follicles during in vitro culture of bovine ovarian tissue</title><author>Cavalcante, Barbara N. ; Matos‐Brito, Bruno G. ; Paulino, Lais R. F. M. ; Silva, Bianca R. ; Aguiar, Antonio Wesley Melo ; Almeida, Edmar Felipe Maia ; Souza, Ana Liza Paz ; Vasconcelos, Gisvani Lopes ; De Assis, Ernando Igo Teixeira ; Silva, Anderson W. B. ; Silva, José Roberto V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3535-7a955c6127f75c41e9fc8ae14347e38b0d608b3f5ba0ab3594ad2e610a53c1403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Activation</topic><topic>Animals</topic><topic>antioxidant</topic><topic>Antioxidants - pharmacology</topic><topic>Cattle</topic><topic>Cell culture</topic><topic>Cell density</topic><topic>Cortex</topic><topic>Cytology</topic><topic>Density</topic><topic>Developmental stages</topic><topic>Female</topic><topic>Follicles</topic><topic>follicular development</topic><topic>Melatonin</topic><topic>Melatonin - pharmacology</topic><topic>Morphology</topic><topic>Ovarian Follicle - drug effects</topic><topic>Ovarian Follicle - growth & development</topic><topic>Stromal cells</topic><topic>Tissue culture</topic><topic>Tissue Culture Techniques - veterinary</topic><topic>Tissues</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cavalcante, Barbara N.</creatorcontrib><creatorcontrib>Matos‐Brito, Bruno G.</creatorcontrib><creatorcontrib>Paulino, Lais R. F. M.</creatorcontrib><creatorcontrib>Silva, Bianca R.</creatorcontrib><creatorcontrib>Aguiar, Antonio Wesley Melo</creatorcontrib><creatorcontrib>Almeida, Edmar Felipe Maia</creatorcontrib><creatorcontrib>Souza, Ana Liza Paz</creatorcontrib><creatorcontrib>Vasconcelos, Gisvani Lopes</creatorcontrib><creatorcontrib>De Assis, Ernando Igo Teixeira</creatorcontrib><creatorcontrib>Silva, Anderson W. B.</creatorcontrib><creatorcontrib>Silva, José Roberto V.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cavalcante, Barbara N.</au><au>Matos‐Brito, Bruno G.</au><au>Paulino, Lais R. F. M.</au><au>Silva, Bianca R.</au><au>Aguiar, Antonio Wesley Melo</au><au>Almeida, Edmar Felipe Maia</au><au>Souza, Ana Liza Paz</au><au>Vasconcelos, Gisvani Lopes</au><au>De Assis, Ernando Igo Teixeira</au><au>Silva, Anderson W. B.</au><au>Silva, José Roberto V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of melatonin on morphology and development of primordial follicles during in vitro culture of bovine ovarian tissue</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Domest Anim</addtitle><date>2019-12</date><risdate>2019</risdate><volume>54</volume><issue>12</issue><spage>1567</spage><epage>1573</epage><pages>1567-1573</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>This study aims to investigate the effect of melatonin on activation, growth and morphology of bovine primordial follicles, as well as on stromal cells density in ovarian tissues after in vitro culture. Ovarian fragments were cultured in α‐MEM+ alone or supplemented with melatonin (250, 500, 1,000 or 2,000 pM) for a period of six days. Non‐cultured and cultured tissues were processed for histological analysis; according to developmental stages, follicles were classified as primordial or growing follicles. These follicles were further classified as morphologically normal or degenerated. Ovarian stromal cell density was also evaluated. The percentages of primordial and developing follicles, as well as those classified of normal follicles, were compared by Fisher's exact test, and the differences were considered significant when p < .05. The results showed that the presence of 1,000 and 2,000 pM melatonin in culture medium promoted a reduction in the percentage of primordial follicles and an increase in the percentage of development follicles, when compared to follicles cultured in control medium. On the other hand, the presence of 250 or 500 pM melatonin did not show a significant effect on the percentage of primordial and developing follicles. Besides that, the presence of 500, 1,000 and 2,000 pM melatonin maintained the percentage of normal follicles similar to those seen uncultured control. Moreover, tissues cultured in presence of 1,000 pM melatonin showed a higher percentage of normal follicles when compared to follicles cultured in the presence of 250 pM melatonin. It was observed a similar profile of stromal density in both uncultured tissues and those cultured in vitro in the presence of melatonin. In conclusion, melatonin (1,000 and 2,000 pM) promotes bovine primordial follicles activation and maintains the stromal cell density during in vitro culture of ovarian cortical tissue.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>31520567</pmid><doi>10.1111/rda.13565</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-5970-6177</orcidid></addata></record> |
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| subjects | Activation Animals antioxidant Antioxidants - pharmacology Cattle Cell culture Cell density Cortex Cytology Density Developmental stages Female Follicles follicular development Melatonin Melatonin - pharmacology Morphology Ovarian Follicle - drug effects Ovarian Follicle - growth & development Stromal cells Tissue culture Tissue Culture Techniques - veterinary Tissues |
| title | Effects of melatonin on morphology and development of primordial follicles during in vitro culture of bovine ovarian tissue |
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