Effects of melatonin on morphology and development of primordial follicles during in vitro culture of bovine ovarian tissue

This study aims to investigate the effect of melatonin on activation, growth and morphology of bovine primordial follicles, as well as on stromal cells density in ovarian tissues after in vitro culture. Ovarian fragments were cultured in α‐MEM+ alone or supplemented with melatonin (250, 500, 1,000 o...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Reproduction in domestic animals 2019-12, Vol.54 (12), p.1567-1573
Hauptverfasser: Cavalcante, Barbara N., Matos‐Brito, Bruno G., Paulino, Lais R. F. M., Silva, Bianca R., Aguiar, Antonio Wesley Melo, Almeida, Edmar Felipe Maia, Souza, Ana Liza Paz, Vasconcelos, Gisvani Lopes, De Assis, Ernando Igo Teixeira, Silva, Anderson W. B., Silva, José Roberto V.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:This study aims to investigate the effect of melatonin on activation, growth and morphology of bovine primordial follicles, as well as on stromal cells density in ovarian tissues after in vitro culture. Ovarian fragments were cultured in α‐MEM+ alone or supplemented with melatonin (250, 500, 1,000 or 2,000 pM) for a period of six days. Non‐cultured and cultured tissues were processed for histological analysis; according to developmental stages, follicles were classified as primordial or growing follicles. These follicles were further classified as morphologically normal or degenerated. Ovarian stromal cell density was also evaluated. The percentages of primordial and developing follicles, as well as those classified of normal follicles, were compared by Fisher's exact test, and the differences were considered significant when p 
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.13565