Performance of basophil activation test and specific IgG4 as diagnostic tools in nonspecific lipid transfer protein allergy: Antwerp‐Barcelona comparison
Background Recent studies show that nsLTP sensitization is not limited to the Mediterranean basin and can present diverse clinical phenotypes. It remains challenging to predict clinical outcome when specific IgE antibodies (sIgE) to nsLTPs are present. This study compares both clinical and in vitro...
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| Veröffentlicht in: | Allergy (Copenhagen) 2020-03, Vol.75 (3), p.616-624 |
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| creator | Decuyper, Ine I. Pascal, Mariona Van Gasse, Athina L. Mertens, Christel Díaz‐Perales, Araceli Araujo, Giovanna Torradeflot, Maria Rius, Josefina Balsells, Sara Muñoz‐Cano, Rosa M. Bartra, Joan Li, Lynne Sabato, Vito Hagendorens, Margo M. Bridts, Chris H. De Clerck, Luc S. Ebo, Didier G. Faber, Margaretha A. |
| description | Background
Recent studies show that nsLTP sensitization is not limited to the Mediterranean basin and can present diverse clinical phenotypes. It remains challenging to predict clinical outcome when specific IgE antibodies (sIgE) to nsLTPs are present. This study compares both clinical and in vitro allergy characteristics but also diagnostic performance of a basophil activation test (BAT) and sIgG4 in nsLTP‐sensitized patients from Antwerp (ANT, Belgium) and Barcelona (BCN, Spain).
Methods
Adult subjects with positive sIgE rPru p 3 and/or rMal d 3 ≥ 0.10 kUA/L (n = 182) and healthy controls (n = 37) were included. NsLTP‐sensitized individuals were stratified according to clinical symptoms with peach/apple, respectively. BAT rPru p 3 and rMal d 3 were performed and sIgG4 antibodies to both components quantified.
Results
In BCN, only ratios of sIgG4/sIgE rMal d 3 and BAT rMal d 3 (0.001 µg/mL) can identify clinically relevant Mal d 3 sensitization (sensitivity of 60%‐63% and a specificity of 75%‐67%, respectively). In ANT, only the sIgE/total IgE rPru p 3 ratio shows added value (sensitivity 60% and specificity 83%). Finally, it appears that symptomatic patients in BCN are more sensitive to lower allergen concentrations compared to ANT. In addition, it was shown that ANT patients were more often sensitized to pollen and that specific pollen sources differed between regions.
Conclusions
NsLTP‐related allergy profiles and diagnostic performance differ significantly between regions and are component‐specific, which makes extrapolation of data difficult to do. In addition, it seems that basophil sensitivity might show geographical differences. Additional research is needed to confirm these findings.
This study explores the performance of BAT, sIgE/total IgE, sIgG4 and sIgG4/sIgE ratio in Pru p 3‐ and Mal d 3‐sensitized populations from Antwerp (ANT) and Barcelona (BCN). In BCN, only ratios of sIgG4/sIgE rMal d 3 and BAT rMal d 3 can identify clinically relevant Mal d 3 sensitization, whereas in ANT, only the sIgE/total IgE rPru p 3 ratio shows added value. NsLTP sensitization and diagnostic performance cannot be extrapolated from one population to another. BAT, basophil activation test; nsLTP, nonspecific lipid transfer protein. |
| doi_str_mv | 10.1111/all.14040 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2289577411</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2375592320</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3530-74c03b31afc8ab2add764cd6f9ff1a825bd8d6124a8ab5777a220c7afc104d4c3</originalsourceid><addsrcrecordid>eNp10btuFDEUBmALgcgSKHgBZIkGikl83ZmhWyIIkVaCAmrrjC-LI4892F6i7XgEet6OJ8GwIQUS01gaf_712wehp5Sc0fadQwhnVBBB7qEV5ePQjeMo76MVoUR2QvLhBD0q5ZoQ0rORPEQnnErKmFyv0I8PNruUZ4ja4uTwBCUtn33AoKv_CtWniKstFUM0uCxWe-c1vtpdCgwFGw-7mEptv2pKoWAfcUzxzgW_eINrhliczXjJqdpGWl-bd4dXeBPrjc3Lz2_fX0PWNqQIWKd5gexLio_RAweh2Ce36yn69PbNx4t33fb95dXFZttpLjnpeqEJnzgFpweYGBjTr4U2azc6R2FgcjKDWVMmoG3Lvu-BMaL7xikRRmh-il4cc1u_L_t2WTX70toEiDbti2JsGNs5QWmjz_-h12mfY2unGO-lHBlnpKmXR6VzKiVbp5bsZ8gHRYn6PTHVXkD9mVizz24T99NszZ38O6IGzo_gxgd7-H-S2my3x8hfAEijqg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2375592320</pqid></control><display><type>article</type><title>Performance of basophil activation test and specific IgG4 as diagnostic tools in nonspecific lipid transfer protein allergy: Antwerp‐Barcelona comparison</title><source>MEDLINE</source><source>Wiley Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Wiley Online Library (Open Access Collection)</source><creator>Decuyper, Ine I. ; Pascal, Mariona ; Van Gasse, Athina L. ; Mertens, Christel ; Díaz‐Perales, Araceli ; Araujo, Giovanna ; Torradeflot, Maria ; Rius, Josefina ; Balsells, Sara ; Muñoz‐Cano, Rosa M. ; Bartra, Joan ; Li, Lynne ; Sabato, Vito ; Hagendorens, Margo M. ; Bridts, Chris H. ; De Clerck, Luc S. ; Ebo, Didier G. ; Faber, Margaretha A.</creator><creatorcontrib>Decuyper, Ine I. ; Pascal, Mariona ; Van Gasse, Athina L. ; Mertens, Christel ; Díaz‐Perales, Araceli ; Araujo, Giovanna ; Torradeflot, Maria ; Rius, Josefina ; Balsells, Sara ; Muñoz‐Cano, Rosa M. ; Bartra, Joan ; Li, Lynne ; Sabato, Vito ; Hagendorens, Margo M. ; Bridts, Chris H. ; De Clerck, Luc S. ; Ebo, Didier G. ; Faber, Margaretha A.</creatorcontrib><description>Background
Recent studies show that nsLTP sensitization is not limited to the Mediterranean basin and can present diverse clinical phenotypes. It remains challenging to predict clinical outcome when specific IgE antibodies (sIgE) to nsLTPs are present. This study compares both clinical and in vitro allergy characteristics but also diagnostic performance of a basophil activation test (BAT) and sIgG4 in nsLTP‐sensitized patients from Antwerp (ANT, Belgium) and Barcelona (BCN, Spain).
Methods
Adult subjects with positive sIgE rPru p 3 and/or rMal d 3 ≥ 0.10 kUA/L (n = 182) and healthy controls (n = 37) were included. NsLTP‐sensitized individuals were stratified according to clinical symptoms with peach/apple, respectively. BAT rPru p 3 and rMal d 3 were performed and sIgG4 antibodies to both components quantified.
Results
In BCN, only ratios of sIgG4/sIgE rMal d 3 and BAT rMal d 3 (0.001 µg/mL) can identify clinically relevant Mal d 3 sensitization (sensitivity of 60%‐63% and a specificity of 75%‐67%, respectively). In ANT, only the sIgE/total IgE rPru p 3 ratio shows added value (sensitivity 60% and specificity 83%). Finally, it appears that symptomatic patients in BCN are more sensitive to lower allergen concentrations compared to ANT. In addition, it was shown that ANT patients were more often sensitized to pollen and that specific pollen sources differed between regions.
Conclusions
NsLTP‐related allergy profiles and diagnostic performance differ significantly between regions and are component‐specific, which makes extrapolation of data difficult to do. In addition, it seems that basophil sensitivity might show geographical differences. Additional research is needed to confirm these findings.
This study explores the performance of BAT, sIgE/total IgE, sIgG4 and sIgG4/sIgE ratio in Pru p 3‐ and Mal d 3‐sensitized populations from Antwerp (ANT) and Barcelona (BCN). In BCN, only ratios of sIgG4/sIgE rMal d 3 and BAT rMal d 3 can identify clinically relevant Mal d 3 sensitization, whereas in ANT, only the sIgE/total IgE rPru p 3 ratio shows added value. NsLTP sensitization and diagnostic performance cannot be extrapolated from one population to another. BAT, basophil activation test; nsLTP, nonspecific lipid transfer protein.</description><identifier>ISSN: 0105-4538</identifier><identifier>EISSN: 1398-9995</identifier><identifier>DOI: 10.1111/all.14040</identifier><identifier>PMID: 31512256</identifier><language>eng</language><publisher>Denmark: Blackwell Publishing Ltd</publisher><subject>Adult ; Allergens ; Allergies ; Antigens, Plant ; Basophils ; BAT ; Belgium ; Carrier Proteins ; Food allergies ; Food Hypersensitivity ; Humans ; Immunoglobulin E ; Immunoglobulin G ; Immunoglobulins ; Mal d 3 ; Medical diagnosis ; nsLTP allergy ; Phenotypes ; Pollen ; Pru p 3 ; sIgG4 ; Spain - epidemiology</subject><ispartof>Allergy (Copenhagen), 2020-03, Vol.75 (3), p.616-624</ispartof><rights>2020 EAACI and John Wiley and Sons A/S. Published by John Wiley and Sons Ltd.</rights><rights>Copyright © 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3530-74c03b31afc8ab2add764cd6f9ff1a825bd8d6124a8ab5777a220c7afc104d4c3</citedby><cites>FETCH-LOGICAL-c3530-74c03b31afc8ab2add764cd6f9ff1a825bd8d6124a8ab5777a220c7afc104d4c3</cites><orcidid>0000-0002-1093-3627 ; 0000-0001-8566-8285 ; 0000-0002-1945-1173 ; 0000-0002-1277-5052 ; 0000-0002-1321-314X ; 0000-0002-3434-4333 ; 0000-0003-0549-9720 ; 0000-0003-2359-0771 ; 0000-0001-8127-5791 ; 0000-0001-6361-9503 ; 0000-0003-0672-7529</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fall.14040$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fall.14040$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31512256$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Decuyper, Ine I.</creatorcontrib><creatorcontrib>Pascal, Mariona</creatorcontrib><creatorcontrib>Van Gasse, Athina L.</creatorcontrib><creatorcontrib>Mertens, Christel</creatorcontrib><creatorcontrib>Díaz‐Perales, Araceli</creatorcontrib><creatorcontrib>Araujo, Giovanna</creatorcontrib><creatorcontrib>Torradeflot, Maria</creatorcontrib><creatorcontrib>Rius, Josefina</creatorcontrib><creatorcontrib>Balsells, Sara</creatorcontrib><creatorcontrib>Muñoz‐Cano, Rosa M.</creatorcontrib><creatorcontrib>Bartra, Joan</creatorcontrib><creatorcontrib>Li, Lynne</creatorcontrib><creatorcontrib>Sabato, Vito</creatorcontrib><creatorcontrib>Hagendorens, Margo M.</creatorcontrib><creatorcontrib>Bridts, Chris H.</creatorcontrib><creatorcontrib>De Clerck, Luc S.</creatorcontrib><creatorcontrib>Ebo, Didier G.</creatorcontrib><creatorcontrib>Faber, Margaretha A.</creatorcontrib><title>Performance of basophil activation test and specific IgG4 as diagnostic tools in nonspecific lipid transfer protein allergy: Antwerp‐Barcelona comparison</title><title>Allergy (Copenhagen)</title><addtitle>Allergy</addtitle><description>Background
Recent studies show that nsLTP sensitization is not limited to the Mediterranean basin and can present diverse clinical phenotypes. It remains challenging to predict clinical outcome when specific IgE antibodies (sIgE) to nsLTPs are present. This study compares both clinical and in vitro allergy characteristics but also diagnostic performance of a basophil activation test (BAT) and sIgG4 in nsLTP‐sensitized patients from Antwerp (ANT, Belgium) and Barcelona (BCN, Spain).
Methods
Adult subjects with positive sIgE rPru p 3 and/or rMal d 3 ≥ 0.10 kUA/L (n = 182) and healthy controls (n = 37) were included. NsLTP‐sensitized individuals were stratified according to clinical symptoms with peach/apple, respectively. BAT rPru p 3 and rMal d 3 were performed and sIgG4 antibodies to both components quantified.
Results
In BCN, only ratios of sIgG4/sIgE rMal d 3 and BAT rMal d 3 (0.001 µg/mL) can identify clinically relevant Mal d 3 sensitization (sensitivity of 60%‐63% and a specificity of 75%‐67%, respectively). In ANT, only the sIgE/total IgE rPru p 3 ratio shows added value (sensitivity 60% and specificity 83%). Finally, it appears that symptomatic patients in BCN are more sensitive to lower allergen concentrations compared to ANT. In addition, it was shown that ANT patients were more often sensitized to pollen and that specific pollen sources differed between regions.
Conclusions
NsLTP‐related allergy profiles and diagnostic performance differ significantly between regions and are component‐specific, which makes extrapolation of data difficult to do. In addition, it seems that basophil sensitivity might show geographical differences. Additional research is needed to confirm these findings.
This study explores the performance of BAT, sIgE/total IgE, sIgG4 and sIgG4/sIgE ratio in Pru p 3‐ and Mal d 3‐sensitized populations from Antwerp (ANT) and Barcelona (BCN). In BCN, only ratios of sIgG4/sIgE rMal d 3 and BAT rMal d 3 can identify clinically relevant Mal d 3 sensitization, whereas in ANT, only the sIgE/total IgE rPru p 3 ratio shows added value. NsLTP sensitization and diagnostic performance cannot be extrapolated from one population to another. BAT, basophil activation test; nsLTP, nonspecific lipid transfer protein.</description><subject>Adult</subject><subject>Allergens</subject><subject>Allergies</subject><subject>Antigens, Plant</subject><subject>Basophils</subject><subject>BAT</subject><subject>Belgium</subject><subject>Carrier Proteins</subject><subject>Food allergies</subject><subject>Food Hypersensitivity</subject><subject>Humans</subject><subject>Immunoglobulin E</subject><subject>Immunoglobulin G</subject><subject>Immunoglobulins</subject><subject>Mal d 3</subject><subject>Medical diagnosis</subject><subject>nsLTP allergy</subject><subject>Phenotypes</subject><subject>Pollen</subject><subject>Pru p 3</subject><subject>sIgG4</subject><subject>Spain - epidemiology</subject><issn>0105-4538</issn><issn>1398-9995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10btuFDEUBmALgcgSKHgBZIkGikl83ZmhWyIIkVaCAmrrjC-LI4892F6i7XgEet6OJ8GwIQUS01gaf_712wehp5Sc0fadQwhnVBBB7qEV5ePQjeMo76MVoUR2QvLhBD0q5ZoQ0rORPEQnnErKmFyv0I8PNruUZ4ja4uTwBCUtn33AoKv_CtWniKstFUM0uCxWe-c1vtpdCgwFGw-7mEptv2pKoWAfcUzxzgW_eINrhliczXjJqdpGWl-bd4dXeBPrjc3Lz2_fX0PWNqQIWKd5gexLio_RAweh2Ce36yn69PbNx4t33fb95dXFZttpLjnpeqEJnzgFpweYGBjTr4U2azc6R2FgcjKDWVMmoG3Lvu-BMaL7xikRRmh-il4cc1u_L_t2WTX70toEiDbti2JsGNs5QWmjz_-h12mfY2unGO-lHBlnpKmXR6VzKiVbp5bsZ8gHRYn6PTHVXkD9mVizz24T99NszZ38O6IGzo_gxgd7-H-S2my3x8hfAEijqg</recordid><startdate>202003</startdate><enddate>202003</enddate><creator>Decuyper, Ine I.</creator><creator>Pascal, Mariona</creator><creator>Van Gasse, Athina L.</creator><creator>Mertens, Christel</creator><creator>Díaz‐Perales, Araceli</creator><creator>Araujo, Giovanna</creator><creator>Torradeflot, Maria</creator><creator>Rius, Josefina</creator><creator>Balsells, Sara</creator><creator>Muñoz‐Cano, Rosa M.</creator><creator>Bartra, Joan</creator><creator>Li, Lynne</creator><creator>Sabato, Vito</creator><creator>Hagendorens, Margo M.</creator><creator>Bridts, Chris H.</creator><creator>De Clerck, Luc S.</creator><creator>Ebo, Didier G.</creator><creator>Faber, Margaretha A.</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1093-3627</orcidid><orcidid>https://orcid.org/0000-0001-8566-8285</orcidid><orcidid>https://orcid.org/0000-0002-1945-1173</orcidid><orcidid>https://orcid.org/0000-0002-1277-5052</orcidid><orcidid>https://orcid.org/0000-0002-1321-314X</orcidid><orcidid>https://orcid.org/0000-0002-3434-4333</orcidid><orcidid>https://orcid.org/0000-0003-0549-9720</orcidid><orcidid>https://orcid.org/0000-0003-2359-0771</orcidid><orcidid>https://orcid.org/0000-0001-8127-5791</orcidid><orcidid>https://orcid.org/0000-0001-6361-9503</orcidid><orcidid>https://orcid.org/0000-0003-0672-7529</orcidid></search><sort><creationdate>202003</creationdate><title>Performance of basophil activation test and specific IgG4 as diagnostic tools in nonspecific lipid transfer protein allergy: Antwerp‐Barcelona comparison</title><author>Decuyper, Ine I. ; Pascal, Mariona ; Van Gasse, Athina L. ; Mertens, Christel ; Díaz‐Perales, Araceli ; Araujo, Giovanna ; Torradeflot, Maria ; Rius, Josefina ; Balsells, Sara ; Muñoz‐Cano, Rosa M. ; Bartra, Joan ; Li, Lynne ; Sabato, Vito ; Hagendorens, Margo M. ; Bridts, Chris H. ; De Clerck, Luc S. ; Ebo, Didier G. ; Faber, Margaretha A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3530-74c03b31afc8ab2add764cd6f9ff1a825bd8d6124a8ab5777a220c7afc104d4c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adult</topic><topic>Allergens</topic><topic>Allergies</topic><topic>Antigens, Plant</topic><topic>Basophils</topic><topic>BAT</topic><topic>Belgium</topic><topic>Carrier Proteins</topic><topic>Food allergies</topic><topic>Food Hypersensitivity</topic><topic>Humans</topic><topic>Immunoglobulin E</topic><topic>Immunoglobulin G</topic><topic>Immunoglobulins</topic><topic>Mal d 3</topic><topic>Medical diagnosis</topic><topic>nsLTP allergy</topic><topic>Phenotypes</topic><topic>Pollen</topic><topic>Pru p 3</topic><topic>sIgG4</topic><topic>Spain - epidemiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Decuyper, Ine I.</creatorcontrib><creatorcontrib>Pascal, Mariona</creatorcontrib><creatorcontrib>Van Gasse, Athina L.</creatorcontrib><creatorcontrib>Mertens, Christel</creatorcontrib><creatorcontrib>Díaz‐Perales, Araceli</creatorcontrib><creatorcontrib>Araujo, Giovanna</creatorcontrib><creatorcontrib>Torradeflot, Maria</creatorcontrib><creatorcontrib>Rius, Josefina</creatorcontrib><creatorcontrib>Balsells, Sara</creatorcontrib><creatorcontrib>Muñoz‐Cano, Rosa M.</creatorcontrib><creatorcontrib>Bartra, Joan</creatorcontrib><creatorcontrib>Li, Lynne</creatorcontrib><creatorcontrib>Sabato, Vito</creatorcontrib><creatorcontrib>Hagendorens, Margo M.</creatorcontrib><creatorcontrib>Bridts, Chris H.</creatorcontrib><creatorcontrib>De Clerck, Luc S.</creatorcontrib><creatorcontrib>Ebo, Didier G.</creatorcontrib><creatorcontrib>Faber, Margaretha A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Allergy (Copenhagen)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Decuyper, Ine I.</au><au>Pascal, Mariona</au><au>Van Gasse, Athina L.</au><au>Mertens, Christel</au><au>Díaz‐Perales, Araceli</au><au>Araujo, Giovanna</au><au>Torradeflot, Maria</au><au>Rius, Josefina</au><au>Balsells, Sara</au><au>Muñoz‐Cano, Rosa M.</au><au>Bartra, Joan</au><au>Li, Lynne</au><au>Sabato, Vito</au><au>Hagendorens, Margo M.</au><au>Bridts, Chris H.</au><au>De Clerck, Luc S.</au><au>Ebo, Didier G.</au><au>Faber, Margaretha A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Performance of basophil activation test and specific IgG4 as diagnostic tools in nonspecific lipid transfer protein allergy: Antwerp‐Barcelona comparison</atitle><jtitle>Allergy (Copenhagen)</jtitle><addtitle>Allergy</addtitle><date>2020-03</date><risdate>2020</risdate><volume>75</volume><issue>3</issue><spage>616</spage><epage>624</epage><pages>616-624</pages><issn>0105-4538</issn><eissn>1398-9995</eissn><abstract>Background
Recent studies show that nsLTP sensitization is not limited to the Mediterranean basin and can present diverse clinical phenotypes. It remains challenging to predict clinical outcome when specific IgE antibodies (sIgE) to nsLTPs are present. This study compares both clinical and in vitro allergy characteristics but also diagnostic performance of a basophil activation test (BAT) and sIgG4 in nsLTP‐sensitized patients from Antwerp (ANT, Belgium) and Barcelona (BCN, Spain).
Methods
Adult subjects with positive sIgE rPru p 3 and/or rMal d 3 ≥ 0.10 kUA/L (n = 182) and healthy controls (n = 37) were included. NsLTP‐sensitized individuals were stratified according to clinical symptoms with peach/apple, respectively. BAT rPru p 3 and rMal d 3 were performed and sIgG4 antibodies to both components quantified.
Results
In BCN, only ratios of sIgG4/sIgE rMal d 3 and BAT rMal d 3 (0.001 µg/mL) can identify clinically relevant Mal d 3 sensitization (sensitivity of 60%‐63% and a specificity of 75%‐67%, respectively). In ANT, only the sIgE/total IgE rPru p 3 ratio shows added value (sensitivity 60% and specificity 83%). Finally, it appears that symptomatic patients in BCN are more sensitive to lower allergen concentrations compared to ANT. In addition, it was shown that ANT patients were more often sensitized to pollen and that specific pollen sources differed between regions.
Conclusions
NsLTP‐related allergy profiles and diagnostic performance differ significantly between regions and are component‐specific, which makes extrapolation of data difficult to do. In addition, it seems that basophil sensitivity might show geographical differences. Additional research is needed to confirm these findings.
This study explores the performance of BAT, sIgE/total IgE, sIgG4 and sIgG4/sIgE ratio in Pru p 3‐ and Mal d 3‐sensitized populations from Antwerp (ANT) and Barcelona (BCN). In BCN, only ratios of sIgG4/sIgE rMal d 3 and BAT rMal d 3 can identify clinically relevant Mal d 3 sensitization, whereas in ANT, only the sIgE/total IgE rPru p 3 ratio shows added value. NsLTP sensitization and diagnostic performance cannot be extrapolated from one population to another. BAT, basophil activation test; nsLTP, nonspecific lipid transfer protein.</abstract><cop>Denmark</cop><pub>Blackwell Publishing Ltd</pub><pmid>31512256</pmid><doi>10.1111/all.14040</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-1093-3627</orcidid><orcidid>https://orcid.org/0000-0001-8566-8285</orcidid><orcidid>https://orcid.org/0000-0002-1945-1173</orcidid><orcidid>https://orcid.org/0000-0002-1277-5052</orcidid><orcidid>https://orcid.org/0000-0002-1321-314X</orcidid><orcidid>https://orcid.org/0000-0002-3434-4333</orcidid><orcidid>https://orcid.org/0000-0003-0549-9720</orcidid><orcidid>https://orcid.org/0000-0003-2359-0771</orcidid><orcidid>https://orcid.org/0000-0001-8127-5791</orcidid><orcidid>https://orcid.org/0000-0001-6361-9503</orcidid><orcidid>https://orcid.org/0000-0003-0672-7529</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0105-4538 |
| ispartof | Allergy (Copenhagen), 2020-03, Vol.75 (3), p.616-624 |
| issn | 0105-4538 1398-9995 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2289577411 |
| source | MEDLINE; Wiley Journals; EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection) |
| subjects | Adult Allergens Allergies Antigens, Plant Basophils BAT Belgium Carrier Proteins Food allergies Food Hypersensitivity Humans Immunoglobulin E Immunoglobulin G Immunoglobulins Mal d 3 Medical diagnosis nsLTP allergy Phenotypes Pollen Pru p 3 sIgG4 Spain - epidemiology |
| title | Performance of basophil activation test and specific IgG4 as diagnostic tools in nonspecific lipid transfer protein allergy: Antwerp‐Barcelona comparison |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T13%3A02%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Performance%20of%20basophil%20activation%20test%20and%20specific%20IgG4%20as%20diagnostic%20tools%20in%20nonspecific%20lipid%20transfer%20protein%20allergy:%20Antwerp%E2%80%90Barcelona%20comparison&rft.jtitle=Allergy%20(Copenhagen)&rft.au=Decuyper,%20Ine%20I.&rft.date=2020-03&rft.volume=75&rft.issue=3&rft.spage=616&rft.epage=624&rft.pages=616-624&rft.issn=0105-4538&rft.eissn=1398-9995&rft_id=info:doi/10.1111/all.14040&rft_dat=%3Cproquest_cross%3E2375592320%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2375592320&rft_id=info:pmid/31512256&rfr_iscdi=true |