Observation of intracellular protein localization area in a single neuron using gold nanoparticles with a scanning electron microscope

•Protein localization areas in a neuron were observed with SEM.•Proteins were immunologically stained with gold nanoparticles and visualized.•Protein localization areas in SEM images agree well with those found in optical images.•Protein localization areas would not be modified after sample preparat...

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