Synthesis and characterization of TPP/chitosan nanoparticles: Colloidal mechanism of reaction and antifungal effect on C. albicans biofilm formation
In the present study chitosan (Chit) nanoparticles were synthetized by the ionic gelation process, using tripolyphosphate (TPP) as crosslinking agent. The TPP/Chit nanoparticle formation was evaluated by titrations, measuring electrical conductivity (k), zeta potential (ZP), hydrodynamic diameter (D...
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| Veröffentlicht in: | Materials Science & Engineering C 2019-11, Vol.104, p.109885-109885, Article 109885 |
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| creator | de Carvalho, Fabiola Galbiatti Magalhães, Taís Chaves Teixeira, Natália Moreira Gondim, Brenna Louise Cavalcanti Carlo, Hugo Lemes dos Santos, Rogério Lacerda de Oliveira, Alan Reis Denadai, Ângelo Marcio Leite |
| description | In the present study chitosan (Chit) nanoparticles were synthetized by the ionic gelation process, using tripolyphosphate (TPP) as crosslinking agent. The TPP/Chit nanoparticle formation was evaluated by titrations, measuring electrical conductivity (k), zeta potential (ZP), hydrodynamic diameter (Dh), viscosity (η) and heat by isothermal calorimetry (ITC). The antifungal effects were evaluated by C. albicans time-kill assays, inhibition of C. albicans initial adhesion and biofilm formation in comparison with nystatin and chitosan. Conductometric titration exhibited a typical precipitation profile, with an inflection at molar ratio of [TPP]/[Chitmon] ≈ 0.3, suggesting a 1:3.3 stoichiometry. The highest Dh, ZP and η values were shown at the beginning of titrations, due to the intramolecular repulsion between Chit-Chit. With addition of TPP, the values showed gradual reduction, with an intermediary transition at [TPP]/[Chitmon] ≈ 0.16, which was attributed to the partial breakdown of interchain crosslinking and formation of discrete charged aggregates. After this point, reaction should occur by neutralization of these assemblies, causing new reduction in values of Dh, ZP and η until [TPP]/[Chitmon] ≈ 0.3, when they reached their lowest values. ITC experiment also showed the occurrence of two bindings (K1 = 3.6 × 103 and K2 = 7.7 × 104), which were entropy driven. Biological results showed lower C. albicans viability for TPP/Chit over 24 h compared with chitosan and nystatin at MIC and 2 MIC. Moreover, TPP/Chit showed 25–50% inhibition of C. albicans adhesion and biofilm formation. The results showed that TPP/Chit nanoparticles reduced the initial adhesion and biofilm formation of C. albicans and demonstrated potential for use in a formulation for the treatment of oral candidiasis.
[Display omitted]
•TPP reaction with Chit forms gradually smaller structures.•Ion gelation process in the TPP/Chit system gives rise to rigid and resistant particles.•TPP/Chit has very low particle-solvent and particle-particle interactions.•TPP/Chit showed lower C. albicans viability than nystatin at MIC and 2 MIC.•TPP/Chit inhibited C. albicans adhesion and biofilm formation. |
| doi_str_mv | 10.1016/j.msec.2019.109885 |
| format | Article |
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[Display omitted]
•TPP reaction with Chit forms gradually smaller structures.•Ion gelation process in the TPP/Chit system gives rise to rigid and resistant particles.•TPP/Chit has very low particle-solvent and particle-particle interactions.•TPP/Chit showed lower C. albicans viability than nystatin at MIC and 2 MIC.•TPP/Chit inhibited C. albicans adhesion and biofilm formation.</description><identifier>ISSN: 0928-4931</identifier><identifier>EISSN: 1873-0191</identifier><identifier>DOI: 10.1016/j.msec.2019.109885</identifier><identifier>PMID: 31500048</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adhesion ; Antifungal activity ; Antifungal Agents - chemistry ; Antifungal Agents - pharmacology ; Antifungal effects ; Biofilms ; Biofilms - drug effects ; Biological effects ; Calorimetry ; Candida albicans ; Candida albicans - drug effects ; Candidiasis ; Chitosan ; Chitosan - analogs & derivatives ; Chitosan - chemistry ; Colloids - chemistry ; Crosslinking ; Electrical conductivity ; Electrical resistivity ; Entropy ; Fungicides ; Gelation ; Gels - chemistry ; Materials science ; Minimum inhibitory concentration ; Mushrooms ; Nanoparticles ; Nanoparticles - chemistry ; Neutralization ; Nystatin ; Nystatin - chemistry ; Polyphosphates - chemistry ; Reduction ; Stoichiometry ; Titration ; TPP ; Tripolyphosphate ; Viability ; Viscosity ; Zeta potential</subject><ispartof>Materials Science & Engineering C, 2019-11, Vol.104, p.109885-109885, Article 109885</ispartof><rights>2019 Elsevier B.V.</rights><rights>Copyright © 2019 Elsevier B.V. All rights reserved.</rights><rights>Copyright Elsevier BV Nov 2019</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-b80835f06ba22b09482eb6ea55b333df588aa4f78722d96bc73005af2f9e31463</citedby><cites>FETCH-LOGICAL-c470t-b80835f06ba22b09482eb6ea55b333df588aa4f78722d96bc73005af2f9e31463</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0928493118331345$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31500048$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Carvalho, Fabiola Galbiatti</creatorcontrib><creatorcontrib>Magalhães, Taís Chaves</creatorcontrib><creatorcontrib>Teixeira, Natália Moreira</creatorcontrib><creatorcontrib>Gondim, Brenna Louise Cavalcanti</creatorcontrib><creatorcontrib>Carlo, Hugo Lemes</creatorcontrib><creatorcontrib>dos Santos, Rogério Lacerda</creatorcontrib><creatorcontrib>de Oliveira, Alan Reis</creatorcontrib><creatorcontrib>Denadai, Ângelo Marcio Leite</creatorcontrib><title>Synthesis and characterization of TPP/chitosan nanoparticles: Colloidal mechanism of reaction and antifungal effect on C. albicans biofilm formation</title><title>Materials Science & Engineering C</title><addtitle>Mater Sci Eng C Mater Biol Appl</addtitle><description>In the present study chitosan (Chit) nanoparticles were synthetized by the ionic gelation process, using tripolyphosphate (TPP) as crosslinking agent. The TPP/Chit nanoparticle formation was evaluated by titrations, measuring electrical conductivity (k), zeta potential (ZP), hydrodynamic diameter (Dh), viscosity (η) and heat by isothermal calorimetry (ITC). The antifungal effects were evaluated by C. albicans time-kill assays, inhibition of C. albicans initial adhesion and biofilm formation in comparison with nystatin and chitosan. Conductometric titration exhibited a typical precipitation profile, with an inflection at molar ratio of [TPP]/[Chitmon] ≈ 0.3, suggesting a 1:3.3 stoichiometry. The highest Dh, ZP and η values were shown at the beginning of titrations, due to the intramolecular repulsion between Chit-Chit. With addition of TPP, the values showed gradual reduction, with an intermediary transition at [TPP]/[Chitmon] ≈ 0.16, which was attributed to the partial breakdown of interchain crosslinking and formation of discrete charged aggregates. After this point, reaction should occur by neutralization of these assemblies, causing new reduction in values of Dh, ZP and η until [TPP]/[Chitmon] ≈ 0.3, when they reached their lowest values. ITC experiment also showed the occurrence of two bindings (K1 = 3.6 × 103 and K2 = 7.7 × 104), which were entropy driven. Biological results showed lower C. albicans viability for TPP/Chit over 24 h compared with chitosan and nystatin at MIC and 2 MIC. Moreover, TPP/Chit showed 25–50% inhibition of C. albicans adhesion and biofilm formation. The results showed that TPP/Chit nanoparticles reduced the initial adhesion and biofilm formation of C. albicans and demonstrated potential for use in a formulation for the treatment of oral candidiasis.
[Display omitted]
•TPP reaction with Chit forms gradually smaller structures.•Ion gelation process in the TPP/Chit system gives rise to rigid and resistant particles.•TPP/Chit has very low particle-solvent and particle-particle interactions.•TPP/Chit showed lower C. albicans viability than nystatin at MIC and 2 MIC.•TPP/Chit inhibited C. albicans adhesion and biofilm formation.</description><subject>Adhesion</subject><subject>Antifungal activity</subject><subject>Antifungal Agents - chemistry</subject><subject>Antifungal Agents - pharmacology</subject><subject>Antifungal effects</subject><subject>Biofilms</subject><subject>Biofilms - drug effects</subject><subject>Biological effects</subject><subject>Calorimetry</subject><subject>Candida albicans</subject><subject>Candida albicans - drug effects</subject><subject>Candidiasis</subject><subject>Chitosan</subject><subject>Chitosan - analogs & derivatives</subject><subject>Chitosan - chemistry</subject><subject>Colloids - chemistry</subject><subject>Crosslinking</subject><subject>Electrical conductivity</subject><subject>Electrical resistivity</subject><subject>Entropy</subject><subject>Fungicides</subject><subject>Gelation</subject><subject>Gels - chemistry</subject><subject>Materials science</subject><subject>Minimum inhibitory concentration</subject><subject>Mushrooms</subject><subject>Nanoparticles</subject><subject>Nanoparticles - chemistry</subject><subject>Neutralization</subject><subject>Nystatin</subject><subject>Nystatin - chemistry</subject><subject>Polyphosphates - chemistry</subject><subject>Reduction</subject><subject>Stoichiometry</subject><subject>Titration</subject><subject>TPP</subject><subject>Tripolyphosphate</subject><subject>Viability</subject><subject>Viscosity</subject><subject>Zeta potential</subject><issn>0928-4931</issn><issn>1873-0191</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc2KFDEUhYMoTs_oC7iQgBs31ZOf-kmJm6FxVBhwwHEdblI3dpqqpE2qhPE5fGBT9ujChavAzXfOvZxDyAvOtpzx9vKwnTLarWC8L4NeqeYR2XDVyapM-GOyYb1QVd1LfkbOcz4w1irZiafkTPKGMVarDfn5-T7Me8w-UwgDtXtIYGdM_gfMPgYaHb27vb20ez_HDIEGCPEIafZ2xPyG7uI4Rj_ASCcs2uDztEoSFpNVvnpCmL1bwtcCoXNoZ1o-dlsKo_EWQqbGR-fHibqYpt9bn5EnDsaMzx_eC_Ll-t3d7kN18-n9x93VTWXrjs2VUUzJxrHWgBCG9bUSaFqEpjFSysE1SgHUrlOdEEPfGttJxhpwwvUoed3KC_L65HtM8duCedaTzxbHEQLGJWshlGIlxL4v6Kt_0ENcUijXaSHXuGvJmkKJE2VTzDmh08fkJ0j3mjO9dqYPeu1Mr53pU2dF9PLBejETDn8lf0oqwNsTgCWL7x6TztZjsDj4VOLUQ_T_8_8FjKOpUg</recordid><startdate>20191101</startdate><enddate>20191101</enddate><creator>de Carvalho, Fabiola Galbiatti</creator><creator>Magalhães, Taís Chaves</creator><creator>Teixeira, Natália Moreira</creator><creator>Gondim, Brenna Louise Cavalcanti</creator><creator>Carlo, Hugo Lemes</creator><creator>dos Santos, Rogério Lacerda</creator><creator>de Oliveira, Alan Reis</creator><creator>Denadai, Ângelo Marcio Leite</creator><general>Elsevier B.V</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20191101</creationdate><title>Synthesis and characterization of TPP/chitosan nanoparticles: Colloidal mechanism of reaction and antifungal effect on C. albicans biofilm formation</title><author>de Carvalho, Fabiola Galbiatti ; Magalhães, Taís Chaves ; Teixeira, Natália Moreira ; Gondim, Brenna Louise Cavalcanti ; Carlo, Hugo Lemes ; dos Santos, Rogério Lacerda ; de Oliveira, Alan Reis ; Denadai, Ângelo Marcio Leite</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-b80835f06ba22b09482eb6ea55b333df588aa4f78722d96bc73005af2f9e31463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adhesion</topic><topic>Antifungal activity</topic><topic>Antifungal Agents - chemistry</topic><topic>Antifungal Agents - pharmacology</topic><topic>Antifungal effects</topic><topic>Biofilms</topic><topic>Biofilms - drug effects</topic><topic>Biological effects</topic><topic>Calorimetry</topic><topic>Candida albicans</topic><topic>Candida albicans - drug effects</topic><topic>Candidiasis</topic><topic>Chitosan</topic><topic>Chitosan - analogs & derivatives</topic><topic>Chitosan - chemistry</topic><topic>Colloids - chemistry</topic><topic>Crosslinking</topic><topic>Electrical conductivity</topic><topic>Electrical resistivity</topic><topic>Entropy</topic><topic>Fungicides</topic><topic>Gelation</topic><topic>Gels - chemistry</topic><topic>Materials science</topic><topic>Minimum inhibitory concentration</topic><topic>Mushrooms</topic><topic>Nanoparticles</topic><topic>Nanoparticles - chemistry</topic><topic>Neutralization</topic><topic>Nystatin</topic><topic>Nystatin - chemistry</topic><topic>Polyphosphates - chemistry</topic><topic>Reduction</topic><topic>Stoichiometry</topic><topic>Titration</topic><topic>TPP</topic><topic>Tripolyphosphate</topic><topic>Viability</topic><topic>Viscosity</topic><topic>Zeta potential</topic><toplevel>online_resources</toplevel><creatorcontrib>de Carvalho, Fabiola Galbiatti</creatorcontrib><creatorcontrib>Magalhães, Taís Chaves</creatorcontrib><creatorcontrib>Teixeira, Natália Moreira</creatorcontrib><creatorcontrib>Gondim, Brenna Louise Cavalcanti</creatorcontrib><creatorcontrib>Carlo, Hugo Lemes</creatorcontrib><creatorcontrib>dos Santos, Rogério Lacerda</creatorcontrib><creatorcontrib>de Oliveira, Alan Reis</creatorcontrib><creatorcontrib>Denadai, Ângelo Marcio Leite</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Materials Science & Engineering C</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Carvalho, Fabiola Galbiatti</au><au>Magalhães, Taís Chaves</au><au>Teixeira, Natália Moreira</au><au>Gondim, Brenna Louise Cavalcanti</au><au>Carlo, Hugo Lemes</au><au>dos Santos, Rogério Lacerda</au><au>de Oliveira, Alan Reis</au><au>Denadai, Ângelo Marcio Leite</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis and characterization of TPP/chitosan nanoparticles: Colloidal mechanism of reaction and antifungal effect on C. albicans biofilm formation</atitle><jtitle>Materials Science & Engineering C</jtitle><addtitle>Mater Sci Eng C Mater Biol Appl</addtitle><date>2019-11-01</date><risdate>2019</risdate><volume>104</volume><spage>109885</spage><epage>109885</epage><pages>109885-109885</pages><artnum>109885</artnum><issn>0928-4931</issn><eissn>1873-0191</eissn><abstract>In the present study chitosan (Chit) nanoparticles were synthetized by the ionic gelation process, using tripolyphosphate (TPP) as crosslinking agent. The TPP/Chit nanoparticle formation was evaluated by titrations, measuring electrical conductivity (k), zeta potential (ZP), hydrodynamic diameter (Dh), viscosity (η) and heat by isothermal calorimetry (ITC). The antifungal effects were evaluated by C. albicans time-kill assays, inhibition of C. albicans initial adhesion and biofilm formation in comparison with nystatin and chitosan. Conductometric titration exhibited a typical precipitation profile, with an inflection at molar ratio of [TPP]/[Chitmon] ≈ 0.3, suggesting a 1:3.3 stoichiometry. The highest Dh, ZP and η values were shown at the beginning of titrations, due to the intramolecular repulsion between Chit-Chit. With addition of TPP, the values showed gradual reduction, with an intermediary transition at [TPP]/[Chitmon] ≈ 0.16, which was attributed to the partial breakdown of interchain crosslinking and formation of discrete charged aggregates. After this point, reaction should occur by neutralization of these assemblies, causing new reduction in values of Dh, ZP and η until [TPP]/[Chitmon] ≈ 0.3, when they reached their lowest values. ITC experiment also showed the occurrence of two bindings (K1 = 3.6 × 103 and K2 = 7.7 × 104), which were entropy driven. Biological results showed lower C. albicans viability for TPP/Chit over 24 h compared with chitosan and nystatin at MIC and 2 MIC. Moreover, TPP/Chit showed 25–50% inhibition of C. albicans adhesion and biofilm formation. The results showed that TPP/Chit nanoparticles reduced the initial adhesion and biofilm formation of C. albicans and demonstrated potential for use in a formulation for the treatment of oral candidiasis.
[Display omitted]
•TPP reaction with Chit forms gradually smaller structures.•Ion gelation process in the TPP/Chit system gives rise to rigid and resistant particles.•TPP/Chit has very low particle-solvent and particle-particle interactions.•TPP/Chit showed lower C. albicans viability than nystatin at MIC and 2 MIC.•TPP/Chit inhibited C. albicans adhesion and biofilm formation.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>31500048</pmid><doi>10.1016/j.msec.2019.109885</doi><tpages>1</tpages></addata></record> |
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| subjects | Adhesion Antifungal activity Antifungal Agents - chemistry Antifungal Agents - pharmacology Antifungal effects Biofilms Biofilms - drug effects Biological effects Calorimetry Candida albicans Candida albicans - drug effects Candidiasis Chitosan Chitosan - analogs & derivatives Chitosan - chemistry Colloids - chemistry Crosslinking Electrical conductivity Electrical resistivity Entropy Fungicides Gelation Gels - chemistry Materials science Minimum inhibitory concentration Mushrooms Nanoparticles Nanoparticles - chemistry Neutralization Nystatin Nystatin - chemistry Polyphosphates - chemistry Reduction Stoichiometry Titration TPP Tripolyphosphate Viability Viscosity Zeta potential |
| title | Synthesis and characterization of TPP/chitosan nanoparticles: Colloidal mechanism of reaction and antifungal effect on C. albicans biofilm formation |
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